Hypoxic ADSC Exosomes Alleviate Primary Sjögren's Syndrome-Induced Skin Injury via GLRX2 Delivery and Ferroptosis Suppression.

IF 2.9 4区医学 Q3 IMMUNOLOGY

Immunological Investigations Pub Date : 2025-06-10 DOI:10.1080/08820139.2025.2516671

Xin Wang, Fengjiao Wu, Xinmeng Yang, Huimin Yang, Guixia Xu, Ruilian Chen, Qinqin Xiang, Mingming Jin, Chao Sun

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引用次数: 0

Abstract

Background: The aim of the present study was to assess the therapeutic effects of ADSC-Exos for SS-induced skin injury.

Methods: A mouse model of SS was constructed and Exos from ADSCs (Exos) and hypoxia-pretreated ADSCs (HExos) were isolated. The therapeutic effects of Exos were identified using an enzyme-linked immunosorbent assay, immunohistochemistry, and immunofluorescence. High-throughput sequencing (HTS) was employed to identify differentially expressed genes between ADSC-Exos and ADSC-HExos. The results showed that treatment with ADSC-Exos, especially ADSC-HExos, inhibited SS-induced expression of inflammatory factors, ferroptosis, and deposition of reactive oxygen species.

Results: The results of HTS and polymerase chain reaction analysis revealed that GLRX2 plays an important role in protected effects of ADSC-HExo against SS-induced skin injury. In vitro analysis usingHaCaT cells confirmed that GLRX2 inhibited lipopolysaccharide-induced skin injury by inhibiting ferroptosis, as confirmed with the ferroptosis inhibitorFerrostatin-1. GLRX2 upregulation increased the therapeutic effects of ADSC-Exos against skin injury of SS mice.

Conclusion: Moreover,ADSC-HExos effectively promoted collagen I expression, suggesting that ADSC-HExos attenuated primary SS-induced skin injury via GLRX2 delivery and ferroptosis suppression.

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缺氧ADSC外泌体通过GLRX2传递和抑制铁下沉减轻原发性Sjögren综合征诱导的皮肤损伤。

背景：本研究的目的是评估ADSC-Exos对ss诱导的皮肤损伤的治疗效果。方法：建立小鼠SS模型，分别从ADSCs （Exos）和缺氧预处理的ADSCs （HExos）中分离Exos。使用酶联免疫吸附试验、免疫组织化学和免疫荧光鉴定Exos的治疗效果。采用高通量测序技术（High-throughput sequencing， HTS）鉴定ADSC-Exos和ADSC-HExos之间的差异表达基因。结果表明，用ADSC-Exos，特别是ADSC-HExos处理，可以抑制ss诱导的炎症因子表达、铁下垂和活性氧沉积。结果：HTS和聚合酶链反应分析结果显示，GLRX2在ADSC-HExo对ss致皮肤损伤的保护作用中起重要作用。hacat细胞的体外分析证实，GLRX2通过抑制铁下垂来抑制脂多糖诱导的皮肤损伤，与铁下垂抑制剂铁抑素-1证实了这一点。GLRX2上调可增强ADSC-Exos对SS小鼠皮肤损伤的治疗作用。结论：此外，ADSC-HExos可有效促进胶原I的表达，表明ADSC-HExos可通过GLRX2传递和抑制铁下垂来减轻ss诱导的原发性皮肤损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunological Investigations 医学-免疫学

CiteScore

5.50

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.