Differences in Aqueous Humor Cytokine Concentrations According to the Presence of Posterior Subcapsular Cataract: A Prospective Case-Control Study.

IF 2.9 4区医学 Q3 IMMUNOLOGY

Immunological Investigations Pub Date : 2025-05-29 DOI:10.1080/08820139.2025.2512143

Kyu Seong Jo, Ji Eun Song, Yongwoo Lee

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引用次数: 0

Abstract

Background: Posterior subcapsular cataract (PSC) substantially impacts visual function, and prior studies have suggested that inflammatory cytokines may have a role in its development. We aimed to analyze inflammatory cytokine concentrations in the aqueous humor by cataract type and severity, specifically comparing them in relation to the presence of PSC, and to identify risk factors for PSC.

Methods: We prospectively recruited patients undergoing routine cataract surgery. PSC presence was documented via slit-lamp examination and anterior segment photography during cataract diagnosis. Aqueous humor samples were collected prior to surgery and analyzed using quantitative multiplexed antibody assays to measure the concentrations of 10 inflammatory cytokines (interferon-α [IFN-α], IFN-γ, interleukin [IL]-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, and tumor necrosis factor-α). Cytokine concentrations were compared according to PSC status.

Results: Overall,80 eyes of 80 patients were included, 45 with and 35 without PSC. Compared with those in the non-PSC group, patients with PSC showed significantly higher aqueous humor concentrations of IFN-γ (2.764 vs. 2.301 pg/mL, p = .013). No significant correlation was observed between PSC status and the aqueous humor concentrations of other examined inflammatory cytokines.

Conclusion: IFN-γ expression tended to be higher in eyes with PSC than in those without PSC, suggesting a possible role in PSC pathophysiology.

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后囊膜下白内障存在时房水细胞因子浓度的差异：一项前瞻性病例对照研究。

背景：后囊膜下白内障（PSC）严重影响视觉功能，先前的研究表明炎症细胞因子可能在其发展中起作用。我们的目的是分析房水中不同白内障类型和严重程度的炎症细胞因子浓度，特别是比较它们与PSC存在的关系，并确定PSC的危险因素。方法：前瞻性招募常规白内障手术患者。在白内障诊断时，通过裂隙灯检查和前段摄影证实PSC的存在。术前收集房水样品，采用定量多重抗体测定法测定10种炎症因子（干扰素-α [IFN-α]、IFN-γ、白细胞介素[IL]-1α、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-10和肿瘤坏死因子-α）的浓度。根据PSC状态比较细胞因子浓度。结果：共纳入80例患者80只眼，其中45只伴PSC， 35只不伴PSC。与非PSC组相比，PSC患者房水中IFN-γ浓度显著高于非PSC组（2.764 vs 2.301 pg/mL, p = 0.013）。未观察到PSC状态与房水中其他炎症细胞因子浓度之间的显著相关性。结论：IFN-γ在PSC患者的表达高于无PSC患者，提示IFN-γ可能与PSC的病理生理有关。

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来源期刊

Immunological Investigations 医学-免疫学

CiteScore

5.50

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.