转录因子FOXP3在人外周血b细胞亚型（CD19+CD39+和CD19+CD39-）中的表达及其调控功能评价

IF 2.9 4区医学 Q3 IMMUNOLOGY

Immunological Investigations Pub Date : 2025-06-11 DOI:10.1080/08820139.2025.2515411

A Cardenas-Juarez, E E Uresti-Rivera, F Ochoa-González, F I Lira-Hernández, E E Lara-Ramírez, J M Vargas-Morales, B Rivas-Santiago, D P Portales-Peréz, M H García-Hernández

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引用次数: 0

摘要

本研究的目的是评估FOXP3在CD19+CD39+和CD19+CD39- B细胞中的表达，并探讨其潜在的调控作用。方法：取25例志愿者外周血B细胞。采用FACS和RT-qPCR分析CD19+CD39+和CD19+CD39- B细胞中FOXP3 mRNA和蛋白水平的表达。通过抗cd3 /CD28刺激的CD19+CD39+和CD19+CD39- B细胞与PBMC共培养来评估抑制活性，评估T细胞增殖和Th1细胞的百分比。结果：CD19+CD39+ FOXP3+ B细胞比例高于其他表型。CD19+ B细胞中FOXP3与CD39呈正相关。与CD19+CD39- B细胞相比，CD19+CD39+ B细胞中FOXP3 mRNA表达增加。与CD19+CD39+ B细胞相比，CD19+CD39- B细胞的增殖和Th1细胞的百分比降低，IL-10 mRNA的表达更高。B细胞表型与Th1细胞和CRP呈负相关。CD19+CD39-与HOMA-β相关。CD19+CD39+与HbA1c呈负相关。讨论：FOXP3在CD19+CD39-和CD19+CD39+ B淋巴细胞上均有表达。CD19+CD39-细胞IL-10表达水平高，FOXP3 mRNA表达水平低。CD19+CD39- B细胞减少Th1细胞，并与β细胞功能相关。

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Expression of the Transcription Factor FOXP3 in Human Peripheral Blood B-Cell Subtypes (CD19+CD39+ and CD19+CD39-) and Evaluation of Their Regulatory Function.

Introduction: The aim of this study was to evaluate FOXP3 expression in CD19⁺CD39⁺ and CD19⁺CD39^- B cells, and to investigate its potential regulatory role.

Methods: Peripheral B cells were obtained from 25 volunteers. FOXP3 expression at the mRNA and protein levels was analyzed in CD19⁺CD39⁺ and CD19⁺CD39^- B cells by FACS and RT-qPCR. Suppressive activity was assessed through co-cultures of PBMC with CD19⁺CD39⁺ and CD19⁺CD39^- B cells stimulated with anti-CD3/CD28, evaluating T cell proliferation and the percentage of Th1 cells.

Results: The percentage of CD19⁺CD39⁺ FOXP3⁺ B cells was higher compared to other phenotypes. There was a positive correlation between FOXP3 and CD39 in CD19⁺ B cells. FOXP3 mRNA was increased in CD19⁺CD39⁺ B cells compared to CD19⁺CD39^- B cells. CD19⁺CD39^- B cells reduced the proliferation, the percentage of Th1 cells, and expressed higher IL-10 mRNA compared to CD19⁺CD39⁺ B cells. B cell phenotypes were inversely associated with Th1 cells and CRP. CD19⁺CD39^- was associated with HOMA-β. CD19⁺CD39⁺ was inversely associated with HbA1c.

Discussion: FOXP3 is expressed on both CD19⁺CD39^- and CD19⁺CD39⁺ B lymphocytes. CD19⁺CD39^- cells showed high levels of IL-10 and low levels of FOXP3 mRNA. CD19⁺CD39^- B cells decreased the Th1 cells and were associated with β-cell function.

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来源期刊

Immunological Investigations 医学-免疫学

CiteScore

5.50

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.