In vivo最新文献

{"title":"Coenzyme Q10 Alleviates Silicosis Fibrosis <i>via</i> Inhibiting Ferroptosis in Mice.","authors":"Yue Sun, Mengxue Yu, Huning Zhang, Wenyue Zhang, Shengpeng Wen, Sirong Chang, Fei Yang, Guangjun Qi, Xin Ma, Zhihong Liu, Anning Yang, Yideng Jiang, Bin Liu","doi":"10.21873/invivo.13816","DOIUrl":"10.21873/invivo.13816","url":null,"abstract":"<p><strong>Background/aim: </strong>Silicosis, the most severe type of occupational pneumoconiosis, leads to diffuse pulmonary fibrosis without specific therapy. Ferroptosis is triggered by reactive oxygen species (ROS) and Fe²⁺ overload-induced lipid peroxidation, which is involved in the progression of pulmonary fibrosis. As an important coenzyme in the process of aerobic respiration, Coenzyme Q10 (CoQ10) can enhance mitochondrial function and energy supply and reduce malondialdehyde (MDA) to limit the risk of fibrosis. We aimed to clarify whether ferroptosis is involved in the process of coenzyme CoQ10-treated silicosis fibrosis.</p><p><strong>Materials and methods: </strong>C57BL/6J mice were divided in 3 groups (n=6 in each group). In the normal group, mice underwent sham operation; in the silicosis group, mice were tracheally instilled with SiO₂ suspension; in CoQ10 group, mice with silicosis were treated with CoQ10 solution. Histological analyses were performed to assess the lung injury level. Iron content was measured by colorimetry in lung tissue. The levels of MDA in lung tissue were characterized by immunofluorescence staining. The level of alpha smooth muscle actin (α-SMA), Collagen I, GPX4, p53 expression was analyzed by qRT-PCR and western blotting.</p><p><strong>Results: </strong>CoQ10 significantly reduced the mRNA and protein expression levels of α-SMA and collagen I in silicosis lung tissues. It is worth noting that CoQ10 significantly inhibited the accumulation of lipid peroxidation and Fe²⁺ level by increasing the expression of ferroptosis regulatory core enzyme GPX4 and reducing its upstream regulator p53 in silicosis lung tissues.</p><p><strong>Conclusion: </strong>CoQ10 alleviated silicosis fibrosis via inhibiting ferroptosis in mice. This finding is a new perspective for exploring the pathogenesis and treatment for silicosis.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 1","pages":"180-189"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppression of Bone Formation and Resorption by the Deletion of Complex Gangliosides.","authors":"Shota Ichikawa, Yoshitaka Mishima, Mayu Nagao, Gyosuke Sakashita, Koichi Furukawa, Takuma Sato, Ken Miyazawa, Kazunori Hamamura","doi":"10.21873/invivo.13824","DOIUrl":"10.21873/invivo.13824","url":null,"abstract":"<p><strong>Background/aim: </strong>Gangliosides regulate bone formation and resorption. Bone formation is reduced in mice lacking ganglioside GM2/GD2 synthase due to a decrease in osteoblasts. However, the effects of the loss of complex gangliosides by the deletion of both GM2/GD2 and GD3 synthases are unknown. Therefore, we investigated whether deletion of complex gangliosides in mice affected bone metabolism.</p><p><strong>Materials and methods: </strong>Twenty-six double-knockout mice lacking both GM2/GD2 and GD3 synthases (dKO) and 30 wild-type (WT) mice as controls were used. The mass of cancellous bone and bone strength in femurs were determined using three-dimensional micro-computed tomography and three-point bending test, respectively. Bone formation and resorption were assessed using histomorphometrical analysis with hematoxylin and eosin, and tartrate-resistant acid phosphatase (TRAP), respectively. Osteoblast proliferation was determined by bromodeoxyuridine assay and the differentiation into osteoclasts by TRAP staining; mRNA levels of osteoclast differentiation markers [nuclear factor of activated T-cells, cytoplasmic 1 (Nfatc1); Trap; and cathepsin K (Ctsk)] were also determined.</p><p><strong>Results: </strong>Bone mass increased in dKO mice, while bone formation and resorption decrease. In terms of bone strength, breaking displacement significantly increased in dKO mice. Furthermore, the proliferation of osteoblasts was suppressed, and the number of TRAP-positive multinucleated cells was reduced in dKO mice. Treatment with receptor activator of NF-[Formula: see text]B ligand significantly reduced Nfatc1, Trap and Ctsk mRNA levels in macrophages from dKO mice.</p><p><strong>Conclusion: </strong>Bone formation and resorption were reduced by the deletion of genes for complex gangliosides. The slight increase in bone strength in dKO mice may be due to the cancellous bone volume increase in these mice.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 1","pages":"257-266"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Times-to-onset and Outcomes of Lung Adverse Events Associated With Sorafenib Using JADER.","authors":"Yuko Kanbayashi, Rio Tomii, Naru Yamamoto, Haruka Wakabayashi, Miku Anzai, Tadashi Shimizu, Mayako Uchida","doi":"10.21873/invivo.13836","DOIUrl":"10.21873/invivo.13836","url":null,"abstract":"<p><strong>Background/aim: </strong>Despite the seriousness of lung adverse events (AEs) associated with sorafenib, comprehensive data are limited. This study was conducted to examine the disproportionality, times to onset, incidence rates, and outcomes of sorafenib-associated lung AEs, using the Japanese Adverse Drug Event Report database.</p><p><strong>Patients and methods: </strong>Data for the period between April 2004 and May 2023 were analyzed. Data on lung AEs were extracted, and the relative disproportionality of AEs was estimated using reporting odds ratios (RORs).</p><p><strong>Results: </strong>A total of 2,230,863 reports were analyzed, and 8,374 reports of AEs associated with sorafenib, including 381 lung AEs, were identified. Signals were detected for two lung AEs: metastases to the lung and tracheal hemorrhage. Fatal outcomes were observed for both AEs. Histograms of the median times to onset of the two detected lung AE signals showed that AEs occurred from 49 to 275 days after sorafenib administration. Weibull distributions showed that the incidences of these AEs occurred constantly throughout the exposure period (random failure type).</p><p><strong>Conclusion: </strong>This study focused on lung AEs associated with sorafenib, highlighting serious outcomes such as lung metastases and tracheal hemorrhage. Continuous monitoring for these AEs is crucial from treatment initiation through the entire therapy course.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 1","pages":"360-366"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canine Stem Cell-derived Exosomes for Lung Inflammation: Efficacy of Intratracheal <i>Versus</i> Intravenous Administration in an Acute Lung Injury Mouse Model.","authors":"Ha-Na Oh, You-Seok Kim, Ga-Hyun Lim, Jae-Bong Moon, Tae-Hong Yoon, Sung-Youl Kim, Ju-Hyun An","doi":"10.21873/invivo.13821","DOIUrl":"10.21873/invivo.13821","url":null,"abstract":"<p><strong>Background/aim: </strong>Acute lung injury (ALI) is an important pathological process in acute respiratory distress syndrome; however, feasible and effective treatment strategies for ALI are limited. Recent studies have suggested that stem cell-derived exosomes can ameliorate ALI; however, there remains no consensus on the protocols used, including the route of administration. This study aimed to identify the appropriate route of administration of canine stem cell-derived exosomes (cSC-Exos) in ALI. Lipopolysaccharides were used to induce ALI.</p><p><strong>Materials and methods: </strong>Mice with ALI were treated with cSC-Exos by intratracheal instillation or intravenous injection. The efficacy of the route of administration was confirmed by determining the total cell count in the bronchoalveolar lavage fluid and histopathological changes. The treatment mechanism was confirmed by measuring cytokine levels and immune cell changes in M2 macrophages (CD206+ cells) and regulatory T cells (FOXP+ cells).</p><p><strong>Results: </strong>When cSC-Exos were injected, inflammation was alleviated, pro-inflammatory cytokine levels were reduced, and FOXP3+ and CD206+ cells were activated. Following intratracheal instillation, an enhanced inflammation-relieving response was observed.</p><p><strong>Conclusion: </strong>This study compared the effects of stem cell-derived exosomes on alleviating lung inflammation according to injection routes in an ALI mouse model. It was confirmed that direct injection of exosomes into the airway had a greater ability to alleviate lung inflammation than intravenous injection by polarizing M2 macrophages and increasing regulatory T cells.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 1","pages":"228-235"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Hydrogen as a Potential Adjunctive Therapy to Improve Renal Function and Reduce Fatigue in an Elderly Patient With Chronic Comorbidities: A Case Report.","authors":"Yun-Ting Lin, Jeng-Wei Lu, Yi-Jung Ho, Shan-Wen Lui, Ting-Yu Hsieh, Kuang-Yih Wang, Feng-Cheng Liu","doi":"10.21873/invivo.13862","DOIUrl":"10.21873/invivo.13862","url":null,"abstract":"<p><strong>Background/aim: </strong>Hydrogen therapy has demonstrated potential as an antioxidant and anti-inflammatory intervention, particularly in the management of chronic diseases such as chronic kidney disease (CKD) and autoimmune conditions. This case report presents the possible therapeutic benefits of molecular hydrogen capsule treatment in enhancing renal function and alleviating chronic fatigue in an elderly female with coronary artery disease (CAD), type 2 diabetes mellitus (DM) complicated by nephropathy, and systemic lupus erythematosus (SLE). The aim of this study was to investigate the efficacy of adjunctive hydrogen therapy in an elderly patient with multiple chronic comorbidities.</p><p><strong>Case report: </strong>An 89-year-old female with a history of CAD s/p who had undergone coronary artery bypass grafting (CABG) over 40 years ago, type 2 DM complicated by nephropathy, and SLE was admitted with recurrent cellulitis at the saphenous vein donor site from her previous CABG. Despite antibiotic treatment, wound healing remained limited. In January 2023, the patient initiated adjuvant treatment with molecular hydrogen capsules. Following the introduction of hydrogen therapy, her renal function improved, evidenced by a reduction in serum creatinine levels. Chronic fatigue, assessed using the Taiwan Brief Fatigue Inventory (BFI-T), showed significant improvement. Immunological evaluation revealed a series of changes, suggesting that immune modulation may be the mechanism underlying the observed clinical benefits.</p><p><strong>Conclusion: </strong>Hydrogen therapy was associated with improved renal function and a reduced chronic fatigue in this elderly patient with multiple comorbidities, including CAD, DM, and SLE. The case underscores the potential therapeutic role of hydrogen therapy in immune modulation and the management of chronic conditions, suggesting the need for further investigation in clinical settings.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 1","pages":"572-576"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Accuracy and Reliability of CT-based Node-RADS for Esophageal Cancer.","authors":"Jakob Leonhardi, Benedikt Schnarkowski, Matthias Mehdorn, Anne-Kathrin Höhn, Stefan Niebisch, Patrick Plum, Daniel Seehofer, Solveig Tiepolt, Timm Denecke, Hans-Jonas Meyer","doi":"10.21873/invivo.13835","DOIUrl":"10.21873/invivo.13835","url":null,"abstract":"<p><strong>Background/aim: </strong>The recently published Node-Reporting and Data System (Node-RADS) can aid the characterization of lymph nodes in cross-sectional imaging. This study investigated the Node-RADS system in computed tomography (CT) to characterize lymph nodes in esophageal cancer.</p><p><strong>Patients and methods: </strong>Overall, 126 patients (15 female, 11.9%) with a mean age of 62.1±10.4 years comprised the patient sample. All patients underwent resection with curative intent and the lymph nodes were histopathologically analyzed during clinical routine. For every patient, the locoregional lymph nodes were scored in accordance with the Node-RADS classification. For statistical analysis, receiver-operating characteristics (ROC) with area under the curve (AUC) were used to test for diagnostic accuracy; inter-reader variability was assessed with Cohen's kappa.</p><p><strong>Results: </strong>Overall, 54 patients were nodal positive (42.9%), 72 patients were nodal negative (57.1%). Inter-reader agreement was substantial for the overall Node-RADS scoring ([Formula: see text]=0.65, p<0.001). ROC curve analysis for lymph node discrimination (N0 versus N1-3) showed an AUC of 0.69 (95% confidence interval=0.59-0.79). A threshold score of more than 2 resulted in a sensitivity of 0.77 and a specificity of 0.55 for correctly predicting nodal positivity. Node-RADS 1 category had a malignancy rate of 30%, Node-RADS 2 of 14%, Node-RADS 3 of 81%, Node-RADS 4 of 90.1% and Node-RADS 5 of 86.5%.</p><p><strong>Conclusion: </strong>The Node-RADS score on staging CT is associated with the malignancy rate of lymph nodes in patients with EC with only moderate diagnostic accuracy. The inter-reader variability is moderate, which could pose difficulties for translation into clinical routine.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 1","pages":"353-359"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Eradication of Fibrosarcoma With Acquired Ifosfamide Resistance Using Methionine Restriction Combined With Ifosfamide in Nude-mouse Models.","authors":"Sei Morinaga, Qinghong Han, Kohei Mizuta, Byung Mo Kang, Michael Bouvet, Norio Yamamoto, Katsuhiro Hayashi, Hiroaki Kimura, Shinji Miwa, Kentaro Igarashi, Takashi Higuchi, Hiroyuki Tsuchiya, Satoru Demura, Robert M Hoffman","doi":"10.21873/invivo.13809","DOIUrl":"10.21873/invivo.13809","url":null,"abstract":"<p><strong>Background/aim: </strong>Ifosfamide is used clinically with doxorubicin as first-line chemotherapy for soft-tissue sarcoma. However, ifosfamide efficacy for soft-tissue sarcoma is limited due to frequent occurence of ifosfamide resistance and thus more effective therapy is needed. The present study aimed to determine the synergy of recombinant methioninase (rMETase) plus ifosfamide against HT1080 human fibrosarcoma cells in vitro. Additionally, the present study also investigated the efficacy of a methionine-restricted diet combined with ifosfamide in nude-mouse models of ifosfamide-resistant HT1080 (IR-HT1080).</p><p><strong>Materials and methods: </strong>Cell viability for HT1080 human fibrosarcoma cells was determined in four groups in vitro: No treatment control; ifosfamide alone; rMETase alone; and a combination of ifosfamide plus rMETase. HT1080 tumors were established in nude mice subcutaneously. The HT1080 tumor models were treated by administering ifosfamide by intraperitoneal injection twice a week, for a total of 11 doses. Surviving tumors were considered ifosfamide resistant (IR-HT1080). Four groups of IR-HT1080 nude-mouse models were subsequently established: Group 1 was a no-treatment control, Group 2 received ifosfamide, Group 3 was given a methionine-restricted diet (MR), and Group 4 received ifosfamide plus MR. Additionally, two groups of nude mice with parental HT1080 subcutaneous tumors were included: Group 5 was a no-treatment control, and Group 6 received ifosfamide for comparison.</p><p><strong>Results: </strong>The 50% inhibitory concentration (IC₅₀) for ifosfamide against HT1080 cells was 0.38 mM. The IC₅₀ for rMETase was 0.75 U/ml for HT1080 cells (data from [4]). The combination of rMETase (0.75 U/ml) plus ifosfamide (0.38 mM) was synergistic against HT1080 fibrosarcoma cells in vitro. The combination of ifosfamide plus MR eradicated the IR-HT1080 tumors in nude-mouse models, while each treatment alone achieved limited tumor inhibition.</p><p><strong>Conclusion: </strong>The present results suggest the combination of MR and ifosfamide has promising potential for overcoming ifosfamide resistance in future clinical applications.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 1","pages":"120-126"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Institutional Experience on Plan Quality in Stereotactic Radiotherapy Using HyperArc for Brain Metastases.","authors":"Sayaka Kihara, Shingo Ohira, Naoyuki Kanayama, Toshiki Ikawa, Shoki Inui, Masaru Isono, Yuya Nitta, Yoshihiro Ueda, Teiji Nishio, Koji Konishi","doi":"10.21873/invivo.13819","DOIUrl":"10.21873/invivo.13819","url":null,"abstract":"<p><strong>Background/aim: </strong>HyperArc (HA) is an automated planning technique enabling single-isocenter brain stereotactic radiotherapy (SRT); however, dosimetric outcomes may be influenced by the planner's expertise. This study aimed to assess the impact of institutional experience on the plan quality of HA-SRT for both single and multiple brain metastases.</p><p><strong>Materials and methods: </strong>Twenty patients who underwent HA-SRT for single metastasis between 2020 and 2021 comprised the earlier group, while those treated between 2022 and 2024 constituted the later group. For multiple metastases, 40 patients who received HA-SRT from 2020-2024 were divided into earlier and later treatment groups. Dosimetric parameters including gross tumor volume (GTV) doses (D_98% and Dmean), volumes of the normal brain (Brain-GTV V_25Gy and V_30Gy), homogeneity index (HI), gradient index (GI), and total monitor unit (MU) were compared. A linear regression model was used to evaluate the effects of planning target volume (PTV) on volumes of normal brain via interaction between PTV volume and treatment era group (earlier vs. later).</p><p><strong>Results: </strong>The later group exhibited significantly higher D_98% and D_mean values for both single and multiple metastases, while V_25Gy and V_30Gy and GI mean values were comparable. Consequently, mean HI and total MU values increased significantly. Both single and multiple metastases showed significant interaction between PTV volume and treatment era group.</p><p><strong>Conclusion: </strong>Enhanced dosimetric outcomes in the later group suggested that accumulated experience contributed to improve GTV and brain dose in HA SRT. Institutional experience is important to improve the plan quality for SRT even with automatic planning such as HA.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 1","pages":"210-217"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship of Metabolic Activity and αvβ3 Receptor Expression in Aggressive Breast Cancer Subtypes Tumors: A Preliminary Report.","authors":"Toàn Minh Ngô, Tamás Nagy, Zoltán Szoboszlai, Csaba Csikos, Noémi Dénes, Andrea Furka, György Trencsényi, Ildikó Garai","doi":"10.21873/invivo.13814","DOIUrl":"10.21873/invivo.13814","url":null,"abstract":"<p><strong>Background/aim: </strong>Angiogenesis imaging has been a valuable complement to metabolic imaging with 2-deoxy-2-[¹⁸F]fluoroglucose (FDG). In our longitudinal study, we investigated the tumour heterogeneity and the relationship between FDG and [⁶⁸Ga]Ga-NODAGA-c(RGDfK)₂ (RGD) accumulation in breast cancer xenografts.</p><p><strong>Materials and methods: </strong>Two groups of cell lines, a fast-growing (4T1) and a slow-growing cell line (MDA-MB-HER2+), were inoculated into SCID mice. RGD and FDG scans were performed in all mice on separate days at four time points. Assessment of tumour uptake based on positron emission tomography/magnetic resonance imaging images was performed using tumour/muscle ratios with the Muscle-Spacing Correction Method to minimize the partial volume effect of the urinary bladder.</p><p><strong>Results: </strong>In the 4T1 group, both radiopharmaceuticals visualized the highly heterogeneous structure of the tumours and showed correlations with tumour growth. Relative linear correlations between FDG and RGD tumour/muscle ratios were observed in all tumours, evident in both high and low-activity areas of 4T1 tumours. When comparing the two groups of different cell lines, SUV ratios in the 4T1 group were higher, especially with [¹⁸F]F-FDG. Our findings highlight the correlations between FDG and RGD, particularly in aggressive breast cancer.</p><p><strong>Conclusion: </strong>This preliminary study supports the combined use of FDG and RGD PET imaging to better characterize tumor heterogeneity and aggressiveness in breast cancer. The observed correlation between FDG and RGD uptake offers insights into the metabolic and vascular behavior of different cancer subtypes, highlighting distinct patterns in 4T1 and MDA-MB-HER2+ lines. This dual-tracer approach shows promise for tailoring therapies based on tumor subtype, though further studies with larger samples are needed to validate these initial findings.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 1","pages":"160-171"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Panoramic Radiographic Analysis of Age- and Sex-related Variations in Upper Mandibular Morphology: Focus on the Condyle, Sigmoid Notch, and Coronoid Process.","authors":"Florian Dudde, Manfred Giese, Oliver Schuck, Sven Duda, Christina Krüger","doi":"10.21873/invivo.13829","DOIUrl":"10.21873/invivo.13829","url":null,"abstract":"<p><strong>Background/aim: </strong>This study aimed to investigate age- and sex-related morphological variations of the mandibular condyle, sigmoid notch, and coronoid process in a mid-sized German population using panoramic radiographs.</p><p><strong>Patients and methods: </strong>A total of 150 participants (89 males, 61 females) aged between 18 and 80 years were included. Participants were divided by age and sex. Panoramic radiographs were obtained, and the right and left condylar processes, sigmoid notches, and coronoid processes were evaluated for shape variations.</p><p><strong>Results: </strong>Age-related differences were found in the mandibular condyles, with younger individuals exhibiting round-shaped condyles more frequently, while older participants showed a higher prevalence of flat-shaped condyles (p<0.001). Sex-related differences included females displaying more diamond-shaped condyles (p=0.033) and triangular-shaped coronoid processes (p=0.004), whereas males exhibited more round condylar processes.</p><p><strong>Conclusion: </strong>This study highlights significant age- and sex-related morphological variations in the mandibular condyles, sigmoid notches, and coronoid processes. These findings have important clinical implications for the diagnosis and treatment of temporomandibular joint disorders and maxillofacial surgery. Understanding these variations can improve diagnostic accuracy and surgical outcomes in patient-specific care.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 1","pages":"311-317"},"PeriodicalIF":1.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}