In vivo最新文献

{"title":"Cervical Selective Nerve Root Block: Three-dimensional Puncture Planning With Dyna-CT Is Superior to Conventional CT-guidance in an <i>Ex Vivo</i> Model.","authors":"Sebastian Schneider, Hans Ulrich Kerl, Miriam Freundt, Katrin E Herrmann, Christoph Groden, Máté E Maros, Sherif A Mohamed","doi":"10.21873/invivo.13875","DOIUrl":"10.21873/invivo.13875","url":null,"abstract":"<p><strong>Background/aim: </strong>Cervical selective nerve root block (CSNRB) is a widely used percutaneous procedure to diagnose and treat cervical radicular pain. The feasibility of a three-dimensional puncture planning and two-dimensional laser-guidance system has previously been shown in an <i>ex vivo</i> model. The purpose of this study was to further compare this technique to the conventional computed tomography (CT-)-guided approach.</p><p><strong>Materials and methods: </strong>Thirty CSNRBs were performed, each with Dyna-CT and the Syngo iGuide^® laser-guidance system (Artis Zee^® Ceiling, Siemens Medical Solutions, Erlangen, Germany), and with conventional CT-guidance (Somatom Volume Zoom, Siemens Healthcare, Erlangen, Germany) in an <i>ex vivo</i> lamb model. The number of puncture attempts, procedural planning time, puncture time, and trajectory length were evaluated and compared.</p><p><strong>Results: </strong>All 60 punctures were rated as successful. Significantly less puncture attempts were needed with Dyna-CT compared to conventional CT-guidance (<i>p</i><0.0001). Procedural planning time and puncture time were significantly shorter with Dyna-CT (<i>p</i> _plan.t<0.0001 and <i>p</i> _punc.t=0.0004) (median 77 s and 56 s, respectively) than with conventional CT-guidance (median 109 s and 159.5 s, respectively), There were no significant differences in trajectory length (Dyna-CT median 3.18 cm; conventional CT median 3.33 cm, <i>p</i>=0.651).</p><p><strong>Conclusion: </strong>Dyna-CT with Syngo iGuide^® laser-guidance is superior to conventional CT-guidance for CSNRB in an <i>ex vivo</i> model. It significantly shortens the overall procedure time by reducing planning time, puncture time, and puncture attempts.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"713-723"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pancreatic Mixed Acinar-neuroendocrine Carcinoma in a Patient With a Germline <i>PTEN</i> Variant: A Case Report and Genomic Literature Review.","authors":"Yosuke Saito, Shuhei Suzuki, Tomomi Sanomachi, Kaho Kato, Hiroya Otake, Yuko Nishise, Yuta Yamada, Koki Saito, Koshi Takahashi, Ryosuke Kumanishi, Tadahisa Fukui, Takashi Yoshioka","doi":"10.21873/invivo.13921","DOIUrl":"10.21873/invivo.13921","url":null,"abstract":"<p><strong>Background/aim: </strong>Cowden syndrome (CS)/PTEN hamartoma tumor syndrome (PHTS) is a hereditary disorder caused by germline <i>PTEN</i> variants. While patients with CS/PHTS have increased risk of various cancers, pancreatic cancer is not typically associated with this syndrome. We report a rare case of pancreatic mixed acinar-neuroendocrine carcinoma in a patient with a germline <i>PTEN</i> variant, aiming to understand its molecular characteristics and clinical implications.</p><p><strong>Case report: </strong>A male in his late 40s presented with pancreatic cancer and hepatic metastases. His medical history included thyroid cancer and familial gastrointestinal malignancies. Liver biopsy revealed mixed acinar-endocrine carcinoma. Cancer genome profiling identified pathogenic variants in <i>GNAS</i> and <i>TP53</i>, along with a germline <i>PTEN</i> variant (V201fs*1), leading to a diagnosis of CS. Notably, <i>KRAS</i> mutations, commonly found in pancreatic cancer, were absent. The patient showed extreme resistance to multiple chemotherapy regimens, including FOLFIRINOX, gemcitabine plus nab-paclitaxel, and cisplatin plus etoposide, resulting in rapid clinical decline.</p><p><strong>Conclusion: </strong>This case highlights a rare presentation of pancreatic cancer in CS/PHTS with distinct molecular and histological features. The absence of <i>KRAS</i> mutation and presence of germline <i>PTEN</i> variant may have contributed to the aggressive clinical course and treatment resistance. These findings underscore the need for further research into the molecular mechanisms of PTEN-associated pancreatic cancers and the development of targeted therapeutic strategies.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"1173-1181"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Clinicopathological Features on Gastric Cancer Stage According to TNM Classification.","authors":"Oliwia Majewska, Radosław Pach, Paweł Brzewski, Jan Kulig, Piotr Kulig","doi":"10.21873/invivo.13916","DOIUrl":"10.21873/invivo.13916","url":null,"abstract":"<p><strong>Background/aim: </strong>TNM stage is crucial for patients with gastric cancer because curative resection and treatment are only possible in early TNM stages. Therefore, our objective was to assess the association of clinicopathological features with TNM stage in such patients.</p><p><strong>Patients and methods: </strong>The association of age, sex, tumor location and Lauren type with TNM stage was analyzed in 910 patients with gastric cancer.</p><p><strong>Results: </strong>Age, sex, and tumor location did not have any association with TNM stage in univariate nor multivariate analyses (<i>p</i>>0.05). However, compared to the diffuse and mixed types, the intestinal type (as defined by the Lauren classification) presented lower T stage of gastric cancer in the chi-squared test (<i>p</i><0.001) and this association was confirmed in the multinominal log normal model (<i>p</i>=0.001).</p><p><strong>Conclusion: </strong>The histological Lauren type of gastric cancer is associated with lower TNM T stage.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"1112-1121"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the COVID-19 Pandemic on Ophthalmologists Residents Well-being and Training Programs: A Systematic Review.","authors":"Vasiliki Dimaki, Christos P Zampetidis, Efthimios Vasilopoulos, Dimitrios Papakonstantinou, Klio Chatzistefanou, Marilita M Moschos","doi":"10.21873/invivo.13867","DOIUrl":"10.21873/invivo.13867","url":null,"abstract":"<p><strong>Background/aim: </strong>The COVID-19 pandemic significantly impacted residents' well-being and training programs, with potentially severe consequences for specialties like ophthalmology, where residents were often reassigned to non-specialty-related units and tasks. This study aimed to systematically review the pandemic's effects on ophthalmology residents' well-being and training.</p><p><strong>Patients and methods: </strong>A systematic review of the literature was carried out using MEDLINE, Scopus, and EBSCO databases. After removing duplicates, 173 unique records were identified, and 30 were considered as potentially relevant. Of these, 13 studies met the inclusion and exclusion criteria and were further analyzed.</p><p><strong>Results: </strong>The reviewed studies revealed a notable decline in both theoretical and practical aspects of residency training during the pandemic. These impacts were supported by both objective data (<i>e.g.</i>, the number of surgeries carried out during the training), and residents' self-reported experiences. The pandemic also had a negative impact on residents' mental health, as evidenced by comparative analyses of data collected prior and during the pandemic. The studies highlighted a correlation between the severity of training disruptions and the negative effects on mental health.</p><p><strong>Conclusion: </strong>Education programs should be implemented to cover the knowledge gap of those who were residents during the pandemic. Additionally, targeted mental health support initiatives are recommended to mitigate the long-term impacts on residents' psychological well-being.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"621-633"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Claudin 18.2 Is Not a Promising Biomarker for Targeted Immunotherapy in Prostatic Cancers.","authors":"Jina Baek, Young Ran Shim, Mi-Jin Gu","doi":"10.21873/invivo.13884","DOIUrl":"10.21873/invivo.13884","url":null,"abstract":"<p><strong>Background/aim: </strong>Claudin 18.2 (CLDN18.2) has emerged as a highly selective biomarker and its abnormal expression has been reported in various primary malignant tumors. Recently, CLDN18.2 has gained attention as both a prognostic biomarker and a potential therapeutic target in gastric and gastroesophageal junction cancer. The purpose of this study was to investigate CLDN18.2 expression in a large cohort of 168 prostate cancer (PC) tissues, assess its correlation with clinicopathological factors, and evaluate its potential as a therapeutic target for anti-CLDN18.2 therapy.</p><p><strong>Materials and methods: </strong>Immunohistochemical staining was conducted using an approved diagnostic CLDN18.2 immunohistochemistry protocol (Ventana, 43-14A clone). Interpretation was carried out following the general rules for staining evaluation.</p><p><strong>Results: </strong>Non-neoplastic gastric mucosa, used as a positive control for CLDN18.2 immunostaining, exhibited strong membranous staining. In contrast, no CLDN18.2 expression was detected in normal ductal and acinar cells of prostate tissue, nor in any of the 168 variable PC tissues.</p><p><strong>Conclusion: </strong>CLDN18.2 is not expressed in normal prostate tissue or PC, suggesting that it is unlikely to serve as a prognostic marker or a potential target for immunotherapy in PC.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"819-823"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Common Molecular Mechanisms Between Periodontitis and Prostate Cancer: A Network Analysis of Differentially Expressed miRNAs.","authors":"Aylin Kanli, Deniz Sunnetci-Akkoyunlu, Nurhan Kulcu-Sarikaya, Cansu Ugurtaş, Gurler Akpinar, Murat Kasap","doi":"10.21873/invivo.13863","DOIUrl":"10.21873/invivo.13863","url":null,"abstract":"<p><strong>Background/aim: </strong>Prostate cancer is the second leading cause of cancer-related deaths in men. Periodontitis is considered a high-risk factor for prostate cancer, but the genetic mechanism is unclear. This study aims to identify dysregulated miRNAs, their associated genes, signaling pathways, and compounds linking periodontitis to prostate cancer.</p><p><strong>Materials and methods: </strong>The miRNA expression datasets of prostate cancer and periodontitis were obtained from the GEO database. Differentially expressed miRNAs (DEmiRNAs) were identified, and common DEmiRNAs (Co-DEmiRNAs) between both datasets were determined. The Co-DEmiRNA-target network structure and functional analyses, including miRNet 2.0, were performed, encompassing Co-DEmiRNA-gene, Co-DEmiRNA-transcription factor (TF), and Co-DEmiRNA-compound networks. Functional enrichment analysis for Co-DEmiRNA genes and Co-DEmiRNA-TF networks was conducted using KEGG, Reactome pathways, and Gene Ontology (GO). Co-up and co-down DEmiRNAs were validated with TCGA miRNA-seq data.</p><p><strong>Results: </strong>hsa-mir-148a-3p, hsa-mir-148b-5p, and hsa-mir-623 are the top miRNA nodes in Co-DEmiRNA-Target networks. The most significant candidate miRNA dysregulation genes are POU2F1, TMOD3, SCD, PRRC2C, and MAT2A, while the most important dysregulation TF includes TP53, CREB1, DNMT1, E2F1, and EGR1. Arsenic trioxide, gemcitabine, and 1,2,6-tri-O-galloyl-beta-D-glucopyranose are the most correlated compounds. Functional analyses revealed multiple cell signaling pathways, such as NOTCH and CREB phosphorylation, and regulation of processes, such as RNA metabolism and transcription.</p><p><strong>Conclusion: </strong>Our study suggests candidate molecular mechanisms linking periodontitis to prostate cancer, highlighting potential compounds targeting both diseases. These findings provide a foundation for guiding future basic and clinical research.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"795-809"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Delayed Cortisol Peak Effect After Severe Burn Injury: A Prospective Observational Study.","authors":"Maximilian Bamberg, Máté Elod Maros, Maximilian Michael Menger, Johannes Tobias Thiel, Claudius Illg, Tim Viergutz, Armin Kalenka, Johann Fontana","doi":"10.21873/invivo.13891","DOIUrl":"10.21873/invivo.13891","url":null,"abstract":"<p><strong>Background/aim: </strong>In contrast to patients with major trauma and septic shock, little is known about the incidence and relevance of acute adrenal insufficiency among patients with burn injuries. Addressing this data gap, the current study was designed to analyze the spontaneous cortisol stress response after severe burn injury.</p><p><strong>Patients and methods: </strong>The study was designed as a prospective observational analysis. From 09/2023 until 06/2024, all patients admitted to the Intensive Care Unit of the BG Tübingen Burn Center were included. The daily cortisol levels were determined in combination with the daily routine laboratory values between 5 and 6 a.m. from day 1 until 7. The data acquisition included the following parameters: Age, sex, total body surface area (TBSA) burned, Abbreviated Burn Severity Index (ABSI), tracheotomy, inhalation trauma, serum cortisol, interleukin-6 (IL-6), leukocyte count and C-reactive protein (CRP).</p><p><strong>Results: </strong>A total of 19 patients with a mean TBSA burned of 33.58±22.68% were included. The mean cortisol values from days 1 to 4 stayed within the physiological range with a delayed significant increase from day 5 until 7 (day 1: 503.3±279.5 <i>vs.</i> day 7: 812.5±330.6 nmol/l; physiological range=130-630 nmol/l). TBSA burned, ABSI, and third-degree injury failed to demonstrate predictive potential, while the IL-6 and leukocyte courses demonstrated significant correlation with the cortisol course.</p><p><strong>Conclusion: </strong>The findings of the current study align with previous studies suggesting a relative cortisol deficit during the acute stages of severe burn injury, supporting the treatment approach of early low-dose hydrocortisone supplementation in patients with severe burn injury.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"877-884"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Preoperative Immune Prognostic Index for Predicting Outcomes in Patients Undergoing Radical Nephroureterectomy for Upper Tract Urothelial Carcinoma.","authors":"Ken Shibata, Kosuke Iwatani, Y U Imai, Kentaro Yoshihara, Keiichiro Miyajima, Wataru Fukuokaya, Kagenori Ito, Taro Igarashi, Takafumi Yanagisawa, Kojiro Tashiro, Shusuke Tsuzuki, Steffi Kar Kei Yuen, Jeremy Yuen-Chun Teoh, Brendan A Yanada, Yusuke Koike, Tatsuya Shimomura, Hiroki Yamada, Akira Furuta, Jun Miki, Takahiro Kimura, Fumihiko Urabe","doi":"10.21873/invivo.13885","DOIUrl":"10.21873/invivo.13885","url":null,"abstract":"<p><strong>Background/aim: </strong>The lung immune prognostic index (LIPI), which is determined by assessing the derived neutrophil-to-lymphocyte ratio in conjunction with the level of lactate dehydrogenase, predicts outcomes in various cancers. Its utility as a preoperative biomarker in upper tract urothelial carcinoma (UTUC) patients remains unexplored.</p><p><strong>Patients and methods: </strong>This is a retrospective study of UTUC patients who underwent radical nephroureterectomy. Patients were stratified into favorable, intermediate, and poor LIPI groups. Non-urothelial tract recurrence-free survival (NUTRFS) was evaluated using Kaplan-Meier analysis, and Cox regression analyses were performed to identify risk factors for NUTRFS.</p><p><strong>Results: </strong>A total of 567 UTUC patients were included, with 46.4% in the favorable, 44.6% in the intermediate, and 9.0% in the poor (9.0%) LIPI groups. The median participant age was 74 years, with a median follow-up of 26 months. Kaplan-Meier curves demonstrated that NUTRFS was significantly worse in patients with poor and intermediate LIPI scores than in those with favorable LIPI scores. Furthermore, combining LIPI status with pathological stages (pT3-4/ypT2-4) and lymph node metastasis status improved postoperative non-urothelial tract recurrence prognosis assessment.</p><p><strong>Conclusion: </strong>The preoperative LIPI is a prognostic indicator for patients with UTUC undergoing nephroureterectomy. Its predictive accuracy improves when combined with advanced pathological stages and lymph node metastasis status.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"824-833"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Waikato Environment for Knowledge Analysis (WEKA) as a Data Analysis Method Identifying Potential Hematological Parameters for Early Diagnosis of Cervical Cancer.","authors":"Hung-Ming Chiu, Shih-En Lin, Yen-Wei Chu, Chih-Jung Chen","doi":"10.21873/invivo.13909","DOIUrl":"10.21873/invivo.13909","url":null,"abstract":"<p><strong>Background/aim: </strong>The present study explored the use of Waikato Environment for Knowledge Analysis (WEKA) to analyze hematological parameters for distinguishing potential development and progression of cervical cancer. Specifically, we aimed to identify significant biomarkers capable of differentiating atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesions (LSIL) from cervical cancer-negative and advanced conditions such as cervical adenocarcinoma.</p><p><strong>Materials and methods: </strong>Hematological and biochemical data were collected from patients and analyzed using data-mining algorithms available in WEKA. The random forest algorithm was employed to identify patterns among key hematological and biochemical biomarkers, alongside one-way analysis of variance to determine significant alterations in these parameters across cancer-negative, ASC-US, LSIL and adenocarcinoma groups.</p><p><strong>Results: </strong>Random forest was the classifier model that demonstrated superior performance metrics with high recall (1.000) and accuracy (0.843), Matthews correlation coefficient (0.510) and area under the curve (0.708), effectively identifying significant patterns within the datasets. One-way analysis of variance indicated significant alterations in red and white blood cell counts, platelet count, hemoglobin, hematocrit and other white blood cell parameters between cancer-negative, ASC-US, LSIL and adenocarcinoma, emphasizing the role of hematological parameters in identifying progression risk.</p><p><strong>Conclusion: </strong>The consistency in conclusions drawn from data mining and statistical analyses highlight the utility of hematological parameters as potential non-invasive biomarkers for cervical cancer screening and progression monitoring. These findings suggest that integrating machine-learning algorithms, particularly random forest, with hematological analysis might enhance early diagnosis and improve clinical outcomes for patients with cervical abnormalities.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"1042-1053"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imipramine-induced Apoptosis and Metastasis Inhibition in Human Bladder Cancer T24 Cells Through EGFR/ERK/NF-κB Pathway Suppression.","authors":"Wei-Shu Wang, Yu-Chang Liu, Tsai-Lin Lo, Fei-Ting Hsu, Chih-Hung Chiang","doi":"10.21873/invivo.13871","DOIUrl":"10.21873/invivo.13871","url":null,"abstract":"<p><strong>Background/aim: </strong>Bladder cancer is a prevalent malignancy, ranging from superficial forms to more aggressive types that invade the muscle and require extensive treatment. Imipramine, traditionally used as an antidepressant, has shown potential as an anti-cancer agent.</p><p><strong>Materials and methods: </strong>In this study, human bladder cancer T24 cells were treated with varying concentrations of imipramine to evaluate its cytotoxic and apoptotic effects.</p><p><strong>Results: </strong>Imipramine induced cytotoxicity in a dose-dependent manner, significantly increasing apoptosis as shown by Annexin-V/PI staining and TUNEL assay. The drug also up-regulated cleaved caspase-3 and down-regulated the anti-apoptotic factor XIAP. Moreover, imipramine activated both extrinsic/intrinsic apoptotic pathways, evidenced by the increased expression of Fas, FasL, cleaved caspase-8, and cleaved caspase-9, along with mitochondrial dysfunction and ROS production. Imipramine inhibited the migration and invasion of bladder cancer cells, likely through the down-regulation of metastasis-related proteins and suppression of the EGFR/ERK/NF-[Formula: see text]B signaling pathway.</p><p><strong>Conclusion: </strong>Imipramine could be a promising therapeutic agent for bladder cancer.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"669-682"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}