Validation of the Chick Chorioallantoic Membrane (CAM) Model for Biocompatibility Analysis of Biomaterials in the Context of the 3R-cascade: A Pilot Study.

IF 1.8 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

In vivo Pub Date : 2025-05-01 DOI:10.21873/invivo.13977

Mike Barbeck, Kerstin Huebner, Kim Burkhardt, Tonya Andreeva, Rumen Krastev, Reiner Schnettler, Sanja Stojanovic, Stevo Najman, Ole Jung, Regine Schneider-Stock

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引用次数: 0

Abstract

Background/aim: The present study aimed to compare the tissue responses to biomaterials in the chick chorioallantoic membrane (CAM) model with those from the subcutaneous implantation model in rats at an early time point. It was especially investigated whether histopathological scoring according to DIN EN ISO 10993-6 is also possible after biomaterial implantation using the CAM model and to what extent the values differ from the data obtained from small animal experiments.

Materials and methods: Implantation of a xenogeneic bone substitute using the CAM model for 24 h and subcutaneous implantation model in rats up to 10 days post implantation were conducted. Standardized histological and histopathological methods were used to apply for histopathological scoring according to DIN EN ISO 10993-6.

Results: The histological analysis as well as the histo-pathological scoring revealed that the tissue responses to the xenogeneic bone substitute were completely comparable in both organisms with no visible or statistical differences.

Conclusion: We suggest that bioincompatible biomaterials can already be sorted out in the context of THE preclinical in vivo test phase. Such pre-testing before the required small animal tests might clearly contribute to the 3R-concept to reduce the number of animals (REDUCE).

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小鸡绒毛尿囊膜（CAM）模型在3r级联环境下生物材料生物相容性分析的验证：一项初步研究。

背景/目的：本研究旨在比较雏鸡绒毛尿囊膜（CAM）模型和大鼠皮下植入模型在早期时间点对生物材料的组织反应。特别研究了生物材料植入CAM模型后，是否也可以按照DIN EN ISO 10993-6进行组织病理学评分，以及这些值与小动物实验数据的差异有多大。材料与方法：采用异种骨替代物CAM模型植入24 h，大鼠皮下植入10 d。根据DIN EN ISO 10993-6，采用标准化的组织学和组织病理学方法进行组织病理学评分。结果：组织学分析和组织病理学评分显示，两种生物对异种骨替代物的组织反应完全可比，无明显差异或统计学差异。结论：我们认为生物不相容的生物材料已经可以在临床前体内试验阶段进行整理。在进行所需的小动物试验之前进行这种预试验，可能明显有助于减少动物数量的3r概念（reduce）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

In vivo 医学-医学：研究与实验

CiteScore

4.20

自引率

4.30%

发文量

330

审稿时长

3-8 weeks

期刊介绍： IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management. The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.