In vivo最新文献

{"title":"An Unexpected Relationship Between Human Papillomavirus and Head and Neck Squamous Cell Carcinoma: A Mendelian Randomization Study.","authors":"Yong Feng, Juan Zhai, Tobias Vollkommer, Martin Gosau, Ralf Smeets, Rico Rutkowski, Jieheng Wu, Liyuan Xiao, Reinhard E Friedrich","doi":"10.21873/invivo.13918","DOIUrl":"10.21873/invivo.13918","url":null,"abstract":"<p><strong>Background/aim: </strong>Head and neck cancer (HNC) is the sixth most common type of cancer worldwide. While it is widely accepted that human papillomavirus (HPV) infection is a crucial risk factor for the development of this type of cancer, there is still a lack of support from large-scale studies. This article addresses this gap by comprehensively analyzing the causal relationship between HPV infection and head and neck cancer using Mendelian randomization methods.</p><p><strong>Patients and methods: </strong>The HPV database was utilized to identify instrumental variables <i>via</i> the most enormous GWAS database search tool. The study examined five cancer groups of data of interest from the UK Biobank and three groups of cancer data from the GAME-ON network, all obtained from public databases. The causal relationship was estimated using various approaches, including IVW, weighted median, MR-Egger, simple mode, and weighted mode.</p><p><strong>Results: </strong>The results indicated no causal relationship between HPV16/18 E7 proteins and head and neck cancer, oral cancer, oropharyngeal cancer, and laryngeal cancer, including UKB database and GEME-ON network. Furthermore, there was no heterogeneity or horizontal pleiotropy in the data.</p><p><strong>Conclusion: </strong>The analysis does not support a causal relationship between HPV infection and HNC, as indicated by the lack of evidence linking HPV16/18 E7 proteins to the development of this cancer.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"1134-1147"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Patient-specific Quality Assurance in Carbon-ion Radiation Therapy: Recalculating Delivered Dose Distribution Using Log Data.","authors":"Yongdo Yun, Min Cheol Han, Changhwan Kim, Chae-Seon Hong, Dong Wook Kim, H O Lee, Hojin Kim, Soorim Han, Hikaru Souda, Sung Hyun Lee, Yuya Miyasaka, Takeo Iwai, Jin Sung Kim","doi":"10.21873/invivo.13913","DOIUrl":"10.21873/invivo.13913","url":null,"abstract":"<p><strong>Background/aim: </strong>Log data from radiation treatment machines can play a crucial role in quality assurance by enabling the recalculation of the delivered dose distribution and identification of deviations in treatment delivery. This article proposes a novel method for recalculating the delivered dose distribution in carbon-ion radiation therapy using log data.</p><p><strong>Materials and methods: </strong>The proposed approach leverages existing functionality in commercial treatment planning systems, thus eliminating the need for specialized in-house software for dose calculation and evaluation. The performed tests entail data generation from actual log files using the Digital Imaging and Communications in Medicine standard, biological dose calculations, and gamma evaluations.</p><p><strong>Results: </strong>The log-based approach demonstrated notable advantages, including improved time efficiency and the ability to calculate three-dimensional biological doses. By recalculating the delivered dose distributions, our method improves quality assurance accuracy in carbon-ion radiation therapy. It complements conventional measurement-based patient-specific quality assurance methods, serving as a valuable addition to the arsenal of tools available for treatment evaluation.</p><p><strong>Conclusion: </strong>We believe that our method has the potential to enhance the efficacy of patient-specific quality assurance and contribute significantly to the advancement of carbon-ion radiation therapy, thereby reinforcing the ongoing evolution of particle therapy.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"1086-1093"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flow Cytometry for Assessing Human Sperm Acrosomal Reaction (AR) and Correlation of AR and Acrosomal Enzyme Activity With Semen Parameters.","authors":"Zubin He, Jinliang Duan, Xin Zhou, Qingdi Quentin Li, Zhuguang Liang, Honglin Chen, Jian Xie, Jiaqiang Luo, Yaobin Wang, Guangzhi Liu, Meifang Zeng","doi":"10.21873/invivo.13896","DOIUrl":"10.21873/invivo.13896","url":null,"abstract":"<p><strong>Background/aim: </strong>This study aimed to evaluate human sperm acrosomal reaction (AR) using flow cytometry and examine the correlation of AR and acrosomal enzyme activity (AEA) with seven semen parameters: total sperm count, concentration, vitality, motility, morphology, survival rate, and sperm DNA fragmentation index (DFI).</p><p><strong>Patients and methods: </strong>A retrospective analysis was conducted on semen data from 398 men in the AR group and 526 men in the AEA group. AR was assessed using flow cytometry, while AEA was measured with the solid-phase BAPNA method. Correlations between AR, AEA, and the seven semen parameters were analyzed. Subgroups based on standard reference values were also compared.</p><p><strong>Results: </strong>AEA demonstrated significant positive correlations with total sperm count, concentration, vitality, motility, morphology, and survival rate, and a negative correlation with DFI (<i>p</i><0.05). In contrast, AR correlated only with sperm concentration. Subgroup analyses revealed that in the AR group, the lower subgroup correlated with concentration and vitality, while the normal subgroup correlated only with concentration. In the AEA group, the normal subgroup showed significant correlations with all seven parameters, while none were found in the lower subgroup. The normal subgroup exhibited significantly higher total count, concentration, vitality, motility, morphology, and survival rate, and lower DFI (<i>p</i><0.05). No significant differences were observed between the normal and lower AR subgroups, except for survival rate (<i>p</i>>0.05).</p><p><strong>Conclusion: </strong>AEA shows stronger and more consistent correlations with semen parameters compared with AR, suggesting its potential as a reliable marker for evaluating male fertility. Further research is needed to clarify the predictive value of AR in male fertility assessment.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"917-926"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of a \"Stomach-preserving Strategy\" for Perforated Gastric Cancer in Patients With Distant Metastasis.","authors":"Masayoshi Terayama, Koshi Kumagai, Motonari Ri, Rie Makuuchi, Masaru Hayami, Satoshi Ida, Manabu Ohashi, Takeshi Sano, Souya Nunobe","doi":"10.21873/invivo.13902","DOIUrl":"10.21873/invivo.13902","url":null,"abstract":"<p><strong>Background/aim: </strong>Gastrectomy is often performed for perforated gastric cancer in patients receiving treatment with curative intent. However, gastrectomy is not a curative procedure, precludes oral intake, and may hinder palliative chemotherapy in patients with metastatic disease. The present study assessed the feasibility of a \"stomach-preserving strategy\" comprising peritoneal lavage and repair surgery for the management of gastric cancer perforation in patients with distant metastasis.</p><p><strong>Patients and methods: </strong>We retrospectively reviewed the medical records of patients with gastric cancer who underwent surgical treatment at our hospital from 2013 to 2021. The clinical courses of patients who had undergone peritoneal lavage and repair surgery for perforated gastric cancer with distant metastasis were reviewed to evaluate postoperative outcomes.</p><p><strong>Results: </strong>During the study period, 3,862 patients underwent radical gastrectomy. Additionally, nine patients with stage IV gastric cancer with distant metastasis prior to treatment underwent emergency surgery due to gastric perforation. Of the nine patients that underwent emergency surgery, seven patients underwent peritoneal lavage and repair surgery and two underwent peritoneal lavage only. No cases of secondary leakage were observed. Seven patients (78%) had a good postoperative course including the resumption of meals and continuation of chemotherapy. The remaining two died of sepsis. The median overall survival time was five months from surgery and 12 months from the initiation of palliative chemotherapy.</p><p><strong>Conclusion: </strong>A \"stomach-preserving strategy\" for the management of perforated gastric cancer is safe in patients with stage IV gastric cancer with distant metastasis and allows continuation of oral intake and palliative chemotherapy.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"969-975"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secretome from HMGB1 Box A-over-expressing Adipose-derived Stem Cells Shows Potential for Skin Rejuvenation by Senescence Reversal in PM2.5-induced Senescence Cells <i>via</i> Stem Cell Induction.","authors":"Zin Zin Ei, Apiwat Mutirangura, Pithi Chanvorachote","doi":"10.21873/invivo.13881","DOIUrl":"10.21873/invivo.13881","url":null,"abstract":"<p><strong>Background/aim: </strong>Exposure to particulate matter 2.5 (PM2.5) can lead to cellular senescence by generating reactive oxygen species (ROS). Box A, a DNA-binding domain found in HMGB1, is known for its ability to counteract aging characteristics. This study explored whether BoxA-induced adipose-derived stem cells secretome (BoxA-SC) can reverse senescence in DP and HWPc cells.</p><p><strong>Materials and methods: </strong>The stemness characteristics and reversal of senescence by BoxA-SC in PM2.5-induced DP and HWPc cells were assessed at the mRNA level using RT-qPCR and at the protein level using immunofluorescence analysis.</p><p><strong>Results: </strong>BoxA-SC (1:20) treatment for 48 h induced stemness and reversed PM2.5-induced cell senescence in DP and HWPc cells. BoxA-SC significantly reduced senescence markers, including SA-β-gal staining, and decreased mRNA levels of senescence-associated secretory phenotype factors (IL1α, IL7, CXCL1) in PM2.5-induced senescent cells. DP and HWPc cells exposed to PM2.5 exhibited an increase in p21 and p16 mRNA and protein levels, which was reversed by BoxA-SC. BoxA-SC reduced p21 and p16 in DP senescent cells approximately 3- and 2-fold, respectively, compared to untreated senescent cells.</p><p><strong>Conclusion: </strong>BoxA-SC can potentially reverse cellular senescence, highlighting the therapeutic potential of stem cells in skin rejuvenation and anti-aging treatments.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"766-777"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of Coplanar VMAT for Brain Metastases Using Halcyon With Knowledge-based Planning from Non-coplanar Plan.","authors":"Yusuke Sakai, Kazuki Kubo, Kenji Matsumoto, Makoto Hosono, Hajime Monzen","doi":"10.21873/invivo.13893","DOIUrl":"10.21873/invivo.13893","url":null,"abstract":"<p><strong>Background/aim: </strong>This study aimed to clarify the feasibility of creating coplanar single-isocenter volumetric modulated arc therapy (SI-VMAT) with a Halcyon using a knowledge-based planning system based on non-coplanar plans for treating multiple brain metastases.</p><p><strong>Patients and methods: </strong>A knowledge-based RapidPlan (RP) planning model was built using 32 TrueBeam SI-VMAT plans including one full arc and three non-coplanar partial arcs. To confirm its applicability across different beam geometries using the Halcyon system, the model was validated on the basis of the root-mean-square error (RSME), deviation rate, and absolute differences between estimated and actual dose-volume histograms (DVHs). Dosimetric performance of TrueBeam and Halcyon plans was then compared for 10 patients.</p><p><strong>Results: </strong>The average RSME values for the Halcyon and TrueBeam plans were 0.75±0.40 Gy and 0.46±0.28 Gy, respectively (<i>p</i><0.05). Using the model for Halcyon plan creation, deviations from the estimated DVH boundaries were mainly observed in the low-dose region (<9 Gy), while the actual DVH closely aligned with the estimated DVH in the intermediate- and higher-dose regions. For comparison of dosimetric performance, statistically significant differences were found in the gradient index, conformity index, and normal brain volumes receiving ≥12 Gy, ≥18 Gy, and ≥27 Gy. However, these differences were deemed clinically negligible, with less than 1% disparity in the above doses to the normal brain.</p><p><strong>Conclusion: </strong>The RP model can effectively predict doses for Halcyon, regardless of different beam configurations. Coplanar SI-VMAT with Halcyon can feasibly be used in clinical applications, such as the treatment of multiple brain metastases.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"894-901"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Diode Laser Irradiation on Bone Formation in the Tibiae of Estrogen-deficient Rats.","authors":"Toshiko Furukawa, Munemitsu Kawano, Satoshi Yokose, Rina Iwamoto, Nobuyuki Udagawa","doi":"10.21873/invivo.13868","DOIUrl":"10.21873/invivo.13868","url":null,"abstract":"<p><strong>Background/aim: </strong>The aim of this study was to examine the effects of diode laser irradiation (910 nm) on bone formation in tibiae with bone defects in estrogen-deficient rats. Micro-computed tomography (micro-CT) was performed for 3-dimensional (3D) morphological evaluation of newly formed bone tissues.</p><p><strong>Materials and methods: </strong>Rats underwent sham operation (Sham) or ovariectomy (OVX), and bone defects were created in the tibiae, which were then subjected to diode laser irradiation for seven days in the laser groups (Sham-laser or OVX-laser groups). Tibiae with bone defects from Sham or OVX groups were irradiated with a guide light instead of a laser, serving as control groups (Sham-Cont or OVX-Cont groups). The tibiae were exposed to laser irradiation every day over a period of seven days. After irradiation, the tibiae underwent micro-CT, and then 3D reconstruction was performed for analysis of new bone formation.</p><p><strong>Results: </strong>Estrogen deficiency induced osteoporosis in the tibiae of OVX rats. Laser irradiation induced greater new bone formation in the region of bone defects in both Sham- and OVX-laser groups compared to the Sham- and OVX-Cont groups, respectively. No significant difference in the volume of new bone formation was seen between Sham- and OVX-laser groups.</p><p><strong>Conclusion: </strong>Laser irradiation could induce new bone formation in the region of bone defects in both Sham and OVX rats. This suggests that laser irradiation has potential for bone regeneration therapy in cases of postmenopausal osteoporosis.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"634-639"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HT1080 Fibrosarcoma With Acquired Trabectedin Resistance: Increased Malignancy But Sustained Sensitivity to Methionine Restriction.","authors":"Sei Morinaga, Qinghong Han, Kohei Mizuta, Byung Mo Kang, Michael Bouvet, Norio Yamamoto, Katsuhiro Hayashi, Hiroaki Kimura, Shinji Miwa, Kentaro Igarashi, Takashi Higuchi, Hiroyuki Tsuchiya, Satoru Demura, Robert M Hoffman","doi":"10.21873/invivo.13872","DOIUrl":"10.21873/invivo.13872","url":null,"abstract":"<p><strong>Background/aim: </strong>Trabectedin is a DNA-binding agent that has shown moderate efficacy for soft-tissue sarcomas. We have previously shown that methionine restriction enhances trabectedin efficacy on both parental and trabectedin-resistant HT1080 (TR-HT1080) cells <i>in vitro</i> The aim of the present study was to determine whether fibrosarcoma cells that acquire trabectedin resistance become more malignant but maintain sensitivity to methionine restriction <i>in vivo</i>Materials and Methods: TR-HT1080 was established by culturing HT1080 cells in stepwise increasing concentrations of trabectedin. An <i>in vitro</i> wound-healing invasion assay was used to compare malignancy of HT1080 and TR-HT1080. <i>In vivo</i>, six groups were established: G1-G4 (TR-HT1080): G1, untreated control; G2, trabectedin treatment; G3, methionine-restricted diet; G4, methionine-restricted diet combined with trabectedin; G5, untreated control of parental HT1080; and G6, trabectedin treatment of parental HT1080.</p><p><strong>Results: </strong>The IC₅₀ of trabectedin was previously determined to be 3.3 nM for the parental HT1080 cells and 42.9 nM for trabectedin-resistant HT1080 cells, representing a 13-fold increase. Wound-healing invasion assays <i>in vitro</i> showed a more rapid wound-closure ratio in TR-HT1080 cells than in parental cells, suggesting increased malignancy compared to the parental cells. The volume of untreated TR-HT1080 tumors grew more rapidly than that of HT1080 tumors, indicating a higher malignancy of TR-HT1080 tumors. The IC₅₀ of recombinant methioninase was previously determined as 0.75 U/ml for HT1080 and 0.93 U/ml for TR-HT1080 cells. Methionine restriction was highly effective on TR-HT1080 tumors, decreasing tumor growth by 4-fold.</p><p><strong>Conclusion: </strong>TR-HT1080 cells acquired high malignancy by <i>in vitro</i> selection for trabectedin resistance. However, methionine restriction overcame trabectedin resistance <i>in vivo</i>, strongly inhibiting tumor growth, which should be further investigated in the clinic.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"683-690"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Expression of NRF2 in the Cortex and Hippocampus Following Bilateral Common Carotid Artery Occlusion.","authors":"Min Seon Choi, Hyo-In Hwang, Yoon Young Chung, Hye-Kyoung Shin, Dong-Joon Kim, Yong Hyun Jun","doi":"10.21873/invivo.13878","DOIUrl":"10.21873/invivo.13878","url":null,"abstract":"<p><strong>Background/aim: </strong>Vascular dementia is the second most common cognitive disorder after Alzheimer's disease. Bilateral common carotid artery occlusion (BCCAO) is a widely used model of vascular dementia associated with chronic cerebral hypoperfusion. Previous studies have reported a beneficial role of nuclear factor erythroid 2-related factor 2 (NRF2) in BCCAO. This study aimed to investigate NRF2 expression in the cortex and hippocampus at 3 and 14 days after BCCAO.</p><p><strong>Materials and methods: </strong>Unoperated male Sprague-Dawley rats were assigned to the control group, while rats that underwent surgery were assigned to the BCCAO group. The right and left common carotid arteries were exposed beneath the esophagus, separated from the vagus nerve and occluded using 4-0 silk sutures. The cerebral cortex and hippocampus were isolated under anesthesia, 3 and 14 days post-surgery. The expression of NRF2 protein was evaluated using western blot analysis.</p><p><strong>Results: </strong>NRF2 expression in the cerebral cortex increased 3 and 14 days after BCCAO, compared to control group. In the hippocampus, NRF2 expression of BCCAO group mice was increased at 3 days, but no difference was observed at day 14 compared to the control group.</p><p><strong>Conclusion: </strong>Chronic hypoperfusion induced by BCCAO altered the protein expression levels of NRF2 in the cortex and hippocampus, suggesting that NRF2 may have a role in cognitive impairment.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"742-748"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Hypoxia on Irisin Secretion by Human Cardiomyocytes.","authors":"Maciej Grzeszczuk, Urszula Ciesielska, Agnieszka Rusak, Michał Jerzy Kulus, Katarzyna Haczkiewicz-Leśniak, Karolina Jabłońska, Alicja Kmiecik, Marzenna Podhorska-Okołów, Piotr Dzięgiel, Katarzyna Nowińska","doi":"10.21873/invivo.13864","DOIUrl":"10.21873/invivo.13864","url":null,"abstract":"<p><strong>Background/aim: </strong>Cardiovascular disease (CVD) is the leading cause of death worldwide, accounting for 31% of all deaths. Biomarkers such as troponins and natriuretic peptides are crucial in diagnosing CVD. Recently, irisin (Ir), a myokine derived from the cleavage of fibronectin type III domain-containing protein 5 (FNDC5), has been identified as a potential new biomarker for CVD. Ir is involved in regulating energy metabolism. This study aimed to determine the expression levels of the <i>FNDC5</i> gene and the level of Ir in cardiomyocytes of the AC16 line subjected to hypoxia.</p><p><strong>Materials and methods: </strong>AC16 cardiomyocytes were cultured under hypoxic conditions for two, four, and six hours. Molecular studies were conducted using western blot, immunofluorescence, RT-PCR, immunoenzymatic test (ELISA), and electron microscopy methods.</p><p><strong>Results: </strong><i>FNDC5</i> gene expression was significantly elevated in cells subjected to hypoxia. Additionally, Ir levels increased in the first hours of hypoxia.</p><p><strong>Conclusion: </strong>Ir could be a potentially useful indicator for assessing CVD risk. Further research is needed to confirm whether elevated Ir levels under hypoxic conditions in AC16 cells represent a promising direction for the development of biomarkers for CVD.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"656-668"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}