In Vitro Cellular & Developmental Biology. Animal最新文献_第4页

Comparison of periapical cyst- and dental pulp-derived mesenchymal stem cells: an in vitro study on proliferation, differentiation, migration, and odontogenic potential. 根尖周囊肿和牙髓源性间充质干细胞的比较：增殖、分化、迁移和成牙潜能的体外研究。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-08-06 DOI: 10.1007/s11626-026-01234-3

Burcu Pirimoğlu, Cangül Keskin, Melek Yüce, Metehan Keskin, Zeyno Nuhoğlu, Esra Albayrak, Nilüfer Özkan, Abdurrahman Aksoy

{"title":"Comparison of periapical cyst- and dental pulp-derived mesenchymal stem cells: an in vitro study on proliferation, differentiation, migration, and odontogenic potential.","authors":"Burcu Pirimoğlu, Cangül Keskin, Melek Yüce, Metehan Keskin, Zeyno Nuhoğlu, Esra Albayrak, Nilüfer Özkan, Abdurrahman Aksoy","doi":"10.1007/s11626-026-01234-3","DOIUrl":"https://doi.org/10.1007/s11626-026-01234-3","url":null,"abstract":"The aim of this study was to isolate human periapical cyst-derived mesenchymal stem cells (PC-MSCs) and compare their immunophenotypic profiles, clonogenic capacity, metabolic activity, migration capacity, and differentiation characteristics with those of dental pulp mesenchymal stem cells (DP-MSCs). PC-MSCs and DP-MSCs were isolated from six periapical cysts and six impacted third molars, respectively. The cells were characterized by flow cytometry and trilineage differentiation assays (osteogenic, adipogenic, chondrogenic). Proliferation was assessed using the MTT assay and colony-forming unit-fibroblast (CFU-F) efficiency. Migration potential was evaluated using an in vitro scratch assay. The secretion of odontogenic markers, dentin sialophosphoprotein (DSPP) and dentin matrix acidic phosphoprotein (DMP1), was quantified using enzyme-linked immunosorbent assay (ELISA). Both cell types expressed characteristic mesenchymal stem cell markers. PC-MSCs exhibited significantly higher colony-forming efficiency (CFU-F, p < 0.05) and superior migration capacity compared to DP-MSCs (p < 0.05). Both MSC populations demonstrated differentiation capacity. DP-MSCs showed more extensive Alizarin Red S staining, while PC-MSCs showed relatively stronger Oil Red O staining. ELISA analysis revealed no statistically significant differences in DSPP and DMP1 secretion levels between the two groups (p > 0.05). Although DP-MSCs exhibited superior mineralizing ability, PC-MSCs displayed higher clonogenic and migratory potential, while no statistically significant differences were observed in DSPP and DMP1 secretion levels between the groups. These findings suggest that periapical cysts, often regarded as pathological waste, may represent a promising and readily accessible source of MSC-like cells with potential relevance for regenerative endodontic research.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148684524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bletilla striata polysaccharide inhibits osteoclast differentiation by suppressing macrophage glycolysis and MMP9 expression. 白芨多糖通过抑制巨噬细胞糖酵解和MMP9表达抑制破骨细胞分化。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-08-06 DOI: 10.1007/s11626-026-01227-2

Zhiya Wang, Yanjie Li, Shu Zhang, Jibo Bao, Peng Ling, Ye Huang, Shuyu Zhu, Zhigang Xie

{"title":"Bletilla striata polysaccharide inhibits osteoclast differentiation by suppressing macrophage glycolysis and MMP9 expression.","authors":"Zhiya Wang, Yanjie Li, Shu Zhang, Jibo Bao, Peng Ling, Ye Huang, Shuyu Zhu, Zhigang Xie","doi":"10.1007/s11626-026-01227-2","DOIUrl":"https://doi.org/10.1007/s11626-026-01227-2","url":null,"abstract":"Utilizing single-cell RNA sequencing and network pharmacology, this study explored the mechanism by which Bletilla striata polysaccharide (BSP) mitigates peri-implantitis-associated osteolysis. Integrated analyses revealed a significant expansion of pro-inflammatory macrophages and osteoclasts in diseased tissues, with differentially expressed genes enriched in glycolytic pathways. Mechanistic exploration indicated that BSP suppresses osteoclastogenesis primarily by targeting matrix metalloproteinase-9 (MMP9), thereby inhibiting proteolytic activity and cytoskeletal remodeling. Preliminary rescue experiments supported this notion, showing that while BSP effectively downregulated LPS-induced MMP9 upregulation and suppressed macrophage glycolytic metabolism, forced overexpression of MMP9 partially restored MMP9 levels and reversed the inhibitory effect of BSP on osteoclast formation. These findings suggest a potential \"metabolism-protease\" interplay, where BSP concurrently modulates immune metabolism and protease activity to alleviate inflammatory bone loss, providing a foundational basis for developing plant-derived polysaccharide therapeutics.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148684392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermal stimulation promotes myogenic differentiation through enhanced glucose uptake and transient lipid droplet accumulation in C2C12 cells. 热刺激通过增强C2C12细胞的葡萄糖摄取和瞬时脂滴积累促进成肌分化。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-08-05 DOI: 10.1007/s11626-026-01232-5

Rumana Mahzabin, Satoko Hayashi, Md Rezwanul Habib, Yukako Tokutake, Shinichi Yonekura

{"title":"Thermal stimulation promotes myogenic differentiation through enhanced glucose uptake and transient lipid droplet accumulation in C2C12 cells.","authors":"Rumana Mahzabin, Satoko Hayashi, Md Rezwanul Habib, Yukako Tokutake, Shinichi Yonekura","doi":"10.1007/s11626-026-01232-5","DOIUrl":"https://doi.org/10.1007/s11626-026-01232-5","url":null,"abstract":"Mild thermal stimulation enhances skeletal muscle differentiation; however, its underlying metabolic basis remains unclear. Here, we demonstrate that thermal stimulation promotes myogenic differentiation through enhanced glucose uptake and transient lipid droplet (LD) accumulation in C2C12 myoblasts. Thermal stimulation at 39 °C induced a transient increase in LD formation during early differentiation. Early transient LD accumulation, particularly on days 1 and 2, was positively correlated with the day 5 fusion index, suggesting that early LD formation is associated with subsequent myogenic differentiation. Suppression of LD formation by Plin2 knockdown impaired myotube formation, indicating that LD formation is functionally required for myogenic differentiation. Thermal stimulation enhanced glucose uptake, accompanied by increased Slc2a4 and Ppargc1a expression and increased GLUT4 fluorescence intensity. Moreover, higher glucose availability further supported LD formation and myogenic differentiation even under serum-free conditions. Notably, mitochondrial mass, ATP content, and oxidative capacity remained largely unchanged, suggesting that enhanced glucose uptake is associated with LD accumulation without a corresponding increase in mitochondrial oxidative capacity. These findings identify transient LD formation as a key metabolic event driving myogenic differentiation under thermal stimulation.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148678460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro replication efficiency of tilapia parvovirus (TiPV) using different fish cell lines. 罗非鱼细小病毒（TiPV）在不同鱼细胞系上的体外复制效率。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-08-01 Epub Date: 2025-11-11 DOI: 10.1007/s11626-025-01127-x

Gani Taju, Seepoo Abdul Majeed, Mohamed Jaffer Abdul Wazith, Sivaraj Mithra, Azeez Sait Sahul Hameed

引用次数: 0

Exploring the potential of RNA interference (RNAi) in mosquito control: from mechanisms to molecular insights. 探索RNA干扰（RNAi）在蚊虫控制中的潜力：从机制到分子见解。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-08-01 Epub Date: 2026-01-26 DOI: 10.1007/s11626-025-01150-y

Sundararajan Balasubramani, Surjeet Kumar Arya, Gayathiri Ekambaram, Dhandapani Gurusamy, Saravanamoorthy Mutharasanallur Duraisamy

{"title":"Exploring the potential of RNA interference (RNAi) in mosquito control: from mechanisms to molecular insights.","authors":"Sundararajan Balasubramani, Surjeet Kumar Arya, Gayathiri Ekambaram, Dhandapani Gurusamy, Saravanamoorthy Mutharasanallur Duraisamy","doi":"10.1007/s11626-025-01150-y","DOIUrl":"10.1007/s11626-025-01150-y","url":null,"abstract":"Mosquito-borne diseases represent a growing global health crisis, exacerbated by climate change and insecticide resistance. RNA interference (RNAi), a natural mechanism of gene silencing, offers a promising, target-specific alternative for mosquito control. This review explores the potential of RNAi to disrupt critical physiological processes, such as reproduction and disease transmission, thereby reducing vector populations and competence. We examine the mechanisms of RNAi, its application in combatting insecticide resistance, and recent advancements in delivery systems, including nanobody- and chitosan-based nanoparticles, which enhance the stability and uptake of double-stranded RNA (dsRNA) molecules. However, significant challenges remain, such as optimizing field-effective delivery methods and assessing potential off-target effects on non-target organisms. Continued innovation in RNAi technology is pivotal for developing sustainable and environmentally sound vector control strategies. This review synthesizes current research, highlighting the molecular insights, practical applications, and future directions for integrating RNAi into modern public health initiatives.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"909-922"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146051878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of cyprinid herpesvirus-2 in goldfish aquarium shops in Tamil Nadu, India: a case report. 印度泰米尔纳德邦金鱼水族商店中鲤疱疹病毒-2的流行：一例报告。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-08-01 Epub Date: 2025-12-15 DOI: 10.1007/s11626-025-01135-x

Kumarasamy Kanimozhi, Allahbagash Badhusha, Gani Taju, Sivaraj Mithra, Seepoo Abdul Majeed, Mohamed Jaffer Abdul Wazith, Venkatesan Rajkumar, Azeez Sait Sahul Hameed

{"title":"Prevalence of cyprinid herpesvirus-2 in goldfish aquarium shops in Tamil Nadu, India: a case report.","authors":"Kumarasamy Kanimozhi, Allahbagash Badhusha, Gani Taju, Sivaraj Mithra, Seepoo Abdul Majeed, Mohamed Jaffer Abdul Wazith, Venkatesan Rajkumar, Azeez Sait Sahul Hameed","doi":"10.1007/s11626-025-01135-x","DOIUrl":"10.1007/s11626-025-01135-x","url":null,"abstract":"This study reports cyprinid herpesvirus-2 infection in farm-reared goldfish in Tamil Nadu during surveillance between 2022 and 2024. CyHV-2 is a temperature-dependent viral pathogen that causes mortality during temperature fluctuations of 18-24°C (post monsoon). Live goldfish showing hemorrhage, skin ulcers, pale gill color, and high mortality rates were collected from aquarium shops in Kolathur, Chennai District, Tamil Nadu, India. To examine pathogenesis, PCR and RT-PCR assays were performed on disease-suspected samples using primer sets for viral infections, such as CyHV-2, CyHV-3, CEV, VHSV, and SVCV. These results confirmed that the CyHV-2 infection caused mortality. CyHV-2 was confirmed by sequence analysis of the ORF92 of CyHV-2 with the reported CyHV-2 strains worldwide. Sequence analysis results showed 97.1 to 100% similarity with the CyHV-2 sequence reported in GenBank. The snakehead kidney cells (CSK) were susceptible to CyHV-2 and replication was confirmed by virus-specific cytopathic effects, PCR, and bioassays. The CyHV-2 was injected in healthy fish through the IM route using viral inoculum derived from infected fish, and the virus was cultivated in susceptible cell lines. Prominent PCR bands indicated that CyHV-2 demonstrated tissue tropism in all the essential organs. The relative expression level of immune-related genes TLR22, HSP70, IL-1β-1, IL-1β, IFNγ-1, TGF-β, and TNF1 was examined in the kidney and spleen of CyHV-2-infected fish using RT-qPCR. During the early stage of infection (48-72 h post-infection), the expression level of TLR22, HSP70, IL-1β, IL-1β-1, IFNγ-1, and TNF1 was significantly upregulated, whereas they were downregulated at 96 h post-infection onwards. In contrast, the TGF-β gene was consistently downregulated throughout the experimental period.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"881-897"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145756306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated assessment of graphene oxide and graphitic carbon nitride nanoparticles: from synthesis to in vitro biological impact on zebrafish (Danio rerio) cell models. 氧化石墨烯和氮化碳纳米颗粒的综合评估：从合成到对斑马鱼细胞模型的体外生物学影响。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-08-01 Epub Date: 2026-04-08 DOI: 10.1007/s11626-026-01178-8

Baskaran Valarmathi, Sivaraj Mithra, Seepoo Abdul Majeed, Kannan Lathika Dharshini, Valiyathur Mohammed Furqaan, Katiyan Mohammed Rehan, Gani Taju, Sakvai Mohammed Safiullah, Kottur Anwar Basha, Sathar Zaheer Ahmed, Amtuz Zehra, Azeez Sait Sahul Hameed

{"title":"Integrated assessment of graphene oxide and graphitic carbon nitride nanoparticles: from synthesis to in vitro biological impact on zebrafish (Danio rerio) cell models.","authors":"Baskaran Valarmathi, Sivaraj Mithra, Seepoo Abdul Majeed, Kannan Lathika Dharshini, Valiyathur Mohammed Furqaan, Katiyan Mohammed Rehan, Gani Taju, Sakvai Mohammed Safiullah, Kottur Anwar Basha, Sathar Zaheer Ahmed, Amtuz Zehra, Azeez Sait Sahul Hameed","doi":"10.1007/s11626-026-01178-8","DOIUrl":"10.1007/s11626-026-01178-8","url":null,"abstract":"Graphene and its derivatives have been increasingly explored for various biomedical applications. Despite their promising potential, the accelerated development and integration of these materials in medical technologies have raised important questions regarding their biocompatibility, toxicity, and overall safety within physiological systems. Graphene oxide (GO) and graphitic carbon nitride (g-C3N4) nanoparticles are widely studied for their strong adsorption and photocatalytic properties, making them effective materials for removing emerging contaminants from water and wastewater. In this study, we performed a detailed evaluation of the acute (24 h) cytotoxic effects of GO and g-C3N4 nanoparticles on DrG and DrF cells by assessing cell morphology, viability, mortality, and membrane integrity. GO exhibited minimal cytotoxicity under the tested conditions, with only a slight reduction in cell viability observed at 100 µg/mL concentrations due to dose-dependent oxidative stress. In contrast, g-C3N4 nanoparticles demonstrated significant cytotoxicity in both cell types, even at 40 µg/mL concentrations, inducing oxidative stress, elevated ROS production, mitochondrial dysfunction, and a marked decrease in cell viability. These findings underscore the importance of dose consideration in the design of GO-based biomedical applications and highlight the potential limitations of g-C3N4 in similar contexts. Overall, GO showed good biocompatibility, further supported by the favorable growth of cells on GO-coated substrates (50 µg/mL).","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"960-975"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147638728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pesticides-induced cellular toxicity in the spinal cord cell line of Lates calcarifer. 农药诱导的calcarifer脊髓细胞系的细胞毒性。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-08-01 Epub Date: 2026-06-04 DOI: 10.1007/s11626-026-01195-7

Baskaran Valarmathi, Sivaraj Mithra, Seepoo Abdul Majeed, Suresh Kalyani Shreya, Siva Divyalakshmi, Gani Taju, Sayeeda Kaneez Fathima, Azeez Sait Sahul Hameed

{"title":"Pesticides-induced cellular toxicity in the spinal cord cell line of Lates calcarifer.","authors":"Baskaran Valarmathi, Sivaraj Mithra, Seepoo Abdul Majeed, Suresh Kalyani Shreya, Siva Divyalakshmi, Gani Taju, Sayeeda Kaneez Fathima, Azeez Sait Sahul Hameed","doi":"10.1007/s11626-026-01195-7","DOIUrl":"10.1007/s11626-026-01195-7","url":null,"abstract":"The extensive use of commercial pesticide formulations, often containing multiple active ingredients, has raised growing concerns about their potential environmental and human health impacts. Among the most widely applied pesticides are imidacloprid (IMI), λ-cyhalothrin (LCT), deltamethrin (DMN), and thiamethoxam (TMX). This study investigated the cytotoxic effects of acute exposure to commercial formulations of these compounds on a seabass spinal cord (SBSC) cell line. Cytotoxicity was evaluated using MTT and Neutral Red (NR) uptake assays, in conjunction with assessments of reactive oxygen species (ROS) generation, plasma membrane staining, lysosomal acidity, fluorescence-based cellular labelling, and effects on neurite-like outgrowth. Effective concentrations (EC10 and EC50) were determined for each pesticide, demonstrating that even low-dose exposure (EC10) significantly disrupted neurite-like outgrowth in SBSC cells. These findings suggest that low-level pesticide exposure may pose a substantial risk to neurodevelopment.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"987-1001"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148155703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of Lates calcarifer primary myoblast proliferation and differentiation from direct cell isolation. 晚期钙化细胞原代成肌细胞增殖和直接细胞分离分化的特征。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-08-01 Epub Date: 2026-05-08 DOI: 10.1007/s11626-026-01185-9

Angela Trace, Miriam Wankell, Craig McFarlane, Lionel Hebbard

引用次数: 0

Derivation and characterization of an embryonic‑derived muscle progenitor cell line from Atlantic salmon (Salmo salar). 大西洋鲑鱼（Salmo salar）胚胎来源的肌肉祖细胞系的衍生和表征。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-08-01 Epub Date: 2026-09-01 DOI: 10.1007/s11626-026-01221-8

Kyle Naylor, Sophia Webb, Deepika Rajesh, Patrick J Mee

{"title":"Derivation and characterization of an embryonic‑derived muscle progenitor cell line from Atlantic salmon (Salmo salar).","authors":"Kyle Naylor, Sophia Webb, Deepika Rajesh, Patrick J Mee","doi":"10.1007/s11626-026-01221-8","DOIUrl":"10.1007/s11626-026-01221-8","url":null,"abstract":"Unlike mammals, teleost fish exhibit lifelong skeletal muscle growth, characterized by continued fiber hypertrophy and the formation of new muscle fibers maintained by a persistent progenitor cell population. However, the limited availability of stable muscle progenitor cell lines from commercially important species such as Atlantic salmon (Salmo salar) constrains mechanistic studies and emerging applications in cellular aquaculture. Here, we report the establishment and characterization of a novel embryonic-derived salmon muscle progenitor cell line, termed SsEC. These cells were derived from late embryonic stages and exhibited a spindle-shaped morphology, robust proliferative capacity, and sustained expansion beyond 30 passages under defined culture conditions. SsECs demonstrated a distinct extracellular matrix preference, with vitronectin supporting long-term maintenance and expansion. Molecular characterization confirmed stable expression of canonical myogenic markers, including myf5 and myod1, while transcriptomic profiling revealed enrichment of genes associated with muscle development and sarcomere organization relative to a non-myogenic salmon cell line. Directed differentiation to muscle, using a two-step protocol, induced efficient formation of multinucleated myotubes expressing myosin heavy chain and sarcomeric α-actinin, with upregulation of key differentiation markers such as myog and tnnt3a. Together, these findings establish SsECs as a robust in vitro model cell line for studying salmon muscle development and provide a novel platform for applications in aquaculture research and cellular seafood production.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"1026-1040"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13582070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148874219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0