In Vitro Cellular & Developmental Biology. Animal最新文献_第10页

Network pharmacology analysis and experimental validation of modified Taohong Siwu decoction against atherosclerosis. 桃红四物汤加味抗动脉粥样硬化网络药理学分析及实验验证。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-04-01 Epub Date: 2026-03-14 DOI: 10.1007/s11626-026-01157-z

Changle Shao, Wanting Meng, Ke Ning, Mengying Huang, Han Li, Hao Cai, Yanwu Xu, Tao Zuo, Hai-Dong Guo

{"title":"Network pharmacology analysis and experimental validation of modified Taohong Siwu decoction against atherosclerosis.","authors":"Changle Shao, Wanting Meng, Ke Ning, Mengying Huang, Han Li, Hao Cai, Yanwu Xu, Tao Zuo, Hai-Dong Guo","doi":"10.1007/s11626-026-01157-z","DOIUrl":"10.1007/s11626-026-01157-z","url":null,"abstract":"The modified Taohong Siwu decoction (MTHSWD), a traditional prescription, demonstrates notable efficacy in treating cardiovascular diseases. However, its potential mechanisms in addressing atherosclerosis (AS) require further exploration. Investigating the underlying mechanisms of MTHSWD in treating AS is imperative. AS mouse models received MTHSWD treatment, with vascular pathology and fibrosis assessed through histopathological staining. Network pharmacology identified bioactive components and potential targets, followed by molecular docking verification of key compound-target interactions. Biochemical validation included Western blot analysis. MTHSWD significantly ameliorated vascular structural abnormalities and fibrosis in AS models. Network pharmacology analysis revealed 133 active components targeting 295 proteins, intersecting with 3309 AS-related genes to form 191 overlapping targets. Twenty-two components (e.g., quercetin) demonstrated multi-target activity (≥ 15 targets). PPI network topology identified 32 hub targets including AKT1, MAPK1/8/14. Functional analysis showed that apoptosis, MAPK signaling, and lipid metabolism pathways were significantly enriched. The pathway-target network highlighted AKT1 and MAPK family members as central regulators. Molecular docking confirmed strong interactions (binding energy < -9.0 kcal/mol) between AKT1-stigmasterol/anhydroicaritin, MAPK1-emodin, and MAPK8/14-β-carotene. Experimental validation demonstrated MTHSWD's regulation of AKT1, MAPK1/14, cleaved-caspase-3, and Bcl-2 expression, enhancing endothelial viability while suppressing apoptosis. This integrated approach reveals that MTHSWD exerts anti-AS effects through multi-target modulation of PI3K/AKT and MAPK signaling pathways, promoting endothelial survival and attenuating apoptotic processes, ultimately alleviating AS-related vascular pathology.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"511-524"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147456898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C-Jun NH2-terminal kinase inhibitor bentamapimod prevents RANKL-mediated osteoclastogenesis and ovariectomy-induced osteoporosis. C-Jun nh2末端激酶抑制剂bentamapimod可预防rankl介导的破骨细胞发生和卵巢切除术引起的骨质疏松症。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-04-01 Epub Date: 2026-02-17 DOI: 10.1007/s11626-025-01147-7

Junming Huang, Mingchao Lin, Kui Deng, Song Zhou

{"title":"C-Jun NH2-terminal kinase inhibitor bentamapimod prevents RANKL-mediated osteoclastogenesis and ovariectomy-induced osteoporosis.","authors":"Junming Huang, Mingchao Lin, Kui Deng, Song Zhou","doi":"10.1007/s11626-025-01147-7","DOIUrl":"10.1007/s11626-025-01147-7","url":null,"abstract":"Over the past five decades, numerous treatment approaches have been developed to target either the inhibition of bone resorption or the promotion of bone formation. However, therapeutic strategies for osteoporosis still face limitations and safety concerns. Bentamapimod, initially designed for neurological disorders, is a novel JNK inhibitor that has demonstrated anti-tumor and immunomodulatory properties. In this study, we aim to investigate the protective role of bentamapimod against bone loss in mice suffering from estrogen deficiency. In vivo, we investigated that bentamapimod alleviated bone loss in mice suffering from estrogen deficiency. According to the micro-CT and histomorphometry assays, bentamapimod inhibits bone resorption as well as bone formation, but the effect of bentamapimod on bone resorption is better than that of bone formation, which ultimately results in ameliorating bone loss caused by ovariectomy. Moreover, we confirmed that bentamapimod can attenuate receptor activator of nuclear factor-kB ligand (RANKL)-induced osteoclast differentiation via inactivating the JNK, while anisomycin (a JNK agonist) partially confronts this effect. Our study demonstrated that bentamapimod would be able to be used as a promising new drug strategy for osteoporosis via inhibiting osteoclast differentiation both in vitro and in vivo.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"476-488"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wound healing properties of Biginelli scaffolds in Tilapia gill cell line: an in vitro analysis and computational approaches. 罗非鱼鳃细胞系Biginelli支架的伤口愈合性能：体外分析和计算方法。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-03-01 Epub Date: 2025-06-19 DOI: 10.1007/s11626-025-01062-x

Noorudeen Paringamalai, Syed Tajudeen Syed Ameen, Abdul Matheen Ibrahim, Gani Taju, Seepoo Abdul Majeed, Azeez Sait Sahul Hameed, Sivaraj Mithra, Predhanekar Mohamed Imran, Attar Kubaib

{"title":"Wound healing properties of Biginelli scaffolds in Tilapia gill cell line: an in vitro analysis and computational approaches.","authors":"Noorudeen Paringamalai, Syed Tajudeen Syed Ameen, Abdul Matheen Ibrahim, Gani Taju, Seepoo Abdul Majeed, Azeez Sait Sahul Hameed, Sivaraj Mithra, Predhanekar Mohamed Imran, Attar Kubaib","doi":"10.1007/s11626-025-01062-x","DOIUrl":"10.1007/s11626-025-01062-x","url":null,"abstract":"The skin is a vital organ that regulates the temperature, nutrient absorption, and perception of sensations. Wound healing is a complex biological process in multicellular systems that consists of four key phases: hemostasis, inflammation, proliferation, and remodeling. This study develops a new approach for synthesizing dihydropyrimidinones (DHPM) named Biginelli scaffolds via a simple, rapid, eco-friendly, and cost-effective solvent-free Biginelli reaction for wound healing activities. The synthesis involved a one-pot three-component coupling reaction of β-ketoester derivatives, anisaldehyde, and simple urea in a domestic microwave oven. The synthesized (B1-B4) scaffolds were characterized using melting point, UV-Vis, FT-IR, HRMS, 2D-NMR (NOESY), and proton/carbon NMR spectroscopies. The molecular docking results showed that the synthetic scaffolds (B1-B4) had strong binding abilities, with B3 and B4 having the best interactions in the group, similar to the control compound (curcumin). It exhibited less cytotoxic effects up to 80 µg/mL in Tilapia gill (TG) cells in the MTT assay. The synthesized scaffolds (60 µg/mL) enhanced TG cell growth and had potential applications in wound healing. Biginelli (B1-B4) scaffolds showed good antioxidant properties in the DPPH assay. RT-qPCR analysis indicated that TG cells exposed to different (B1-B4) scaffold concentrations had significantly increased VEGF gene expression. The scaffolds showed no toxic effects on adsorption, distribution, metabolism, excretion, and toxicity (ADMET) analysis, and the structure was optimized using the DFT-B3LYP-6311G-(d,p) hybrid basis set. This method has wide applications in future research and provides insights into tissue engineering and biomedical applications.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"389-411"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improve your success with fish cell lines-small things that matter. 提高鱼细胞系的成功率——小事情很重要。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-03-01 Epub Date: 2025-04-09 DOI: 10.1007/s11626-025-01042-1

Anita Solhaug, Georgina C Dowd, Vivian R Dayeh, Hilde Sindre, Lucy E J Lee, Niels C Bols

引用次数: 0

Development and characterization of a cell line from the caudal fin of Schizothorax niger (Heckel, 1838) for in vitro toxicity testing. 黑裂胸鱼尾鳍细胞系的发育和特性（Heckel, 1838）用于体外毒性测试。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-03-01 Epub Date: 2025-02-05 DOI: 10.1007/s11626-025-01018-1

Ashaq Sultan Dar, Fayaz Ahmad, Feroz Ahmad Shah, Syed Shariq Nazir Qadiri, Keezia Khurshid

{"title":"Development and characterization of a cell line from the caudal fin of Schizothorax niger (Heckel, 1838) for in vitro toxicity testing.","authors":"Ashaq Sultan Dar, Fayaz Ahmad, Feroz Ahmad Shah, Syed Shariq Nazir Qadiri, Keezia Khurshid","doi":"10.1007/s11626-025-01018-1","DOIUrl":"10.1007/s11626-025-01018-1","url":null,"abstract":"Here, we successfully grew the SNCF (Schizothorax niger caudal fin) cell line from the caudal fin explants of S. niger, an important cold-water fish of the Himalayas. The cells were successfully grown up to 22 passages by planting explant tissues in DMEM medium supplemented with FBS. We observed optimal cell growth at a concentration of 18% FBS. We observed the steady generation of cells from explants from days 2 to 5 of seeding, and obtained a complete monolayer at days 7-10. We tested various temperatures, including 10 °C, 13 °C, 16 °C, 19 °C, 22 °C, 25 °C, and 28 °C, and found that 22 °C was the optimal temperature for cell growth. We examined the response to various doses of epidermal growth factor (EGF) and fibroblast growth factor (FGF) (0, 2, 4, 6, 8, and 10 ng/mL) on cell colony growth at an optimal temperature of 22 °C. We characterized the cell line using karyotyping at the 14th and 20th passages. The cell line showed epithelial cell-like growth by morphology, which was confirmed by immunotyping. We further used the cell line to study the impact of three pesticides (chlorpyrifos, dimethoate, and endosulfan), and a fungicide (mancozeb) and bacterial extracellular product (ECP). The DAPI stain assay and MTT assay confirmed the pesticides toxic effects on the cells, revealing disintegration of the cell nuclei by the formation of micronuclei and LC50 concentrations. ECP treatment showed disruption of the monolayer within 0-36 hrs.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"255-267"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143187760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An in vitro cellular model for measuring the impact of thermal stress on Florida reef sponges. 用于测量热应力对佛罗里达礁海绵影响的体外细胞模型。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-03-01 Epub Date: 2025-05-22 DOI: 10.1007/s11626-025-01034-1

Megan Conkling, Tobin Hindle, Zhixiao Xie, Weibo Liu, Timothy Moore, Shirley A Pomponi

{"title":"An in vitro cellular model for measuring the impact of thermal stress on Florida reef sponges.","authors":"Megan Conkling, Tobin Hindle, Zhixiao Xie, Weibo Liu, Timothy Moore, Shirley A Pomponi","doi":"10.1007/s11626-025-01034-1","DOIUrl":"10.1007/s11626-025-01034-1","url":null,"abstract":"Coral reefs are threatened by recurrent mortality incidents in their native habitats brought on by natural and anthropogenic stressors. Elevated temperature has been indicated as a major causing factor. Although ongoing research is focused on corals, sponges are an important benthic organism on coral reefs and are often overlooked. An accurate and standardized method is needed to determine the environmental limits and thresholds of sponges commonly found on coral reefs. We established an in vitro sponge cell model and evaluated the effect of elevated temperatures on primary cell cultures of five common Florida reef sponges-Agelas clathrodes, Aplysina fulva, Cliona varians, Geodia neptuni, and Xestospongia muta. Analysis of the results revealed that the impact of increased temperatures had no significant effect at the cellular level, but there are changes at the molecular level. Shifts in the sponges' transcriptomic profiles induced by increased temperatures, trigger processes related to signal transduction, apoptosis, and cell repair pathways. Further elevation of temperature corresponding to local extremes activated the immune response and programmed cell death. The results of the present study are based on both cellular and molecular data obtained from the in vitro cell model which highlight the minimal response of all five species to thermal stress, providing an insight into the mechanisms involved in the adaptive process. Furthermore, they suggest a resilience of these sponges to the current thermal extremes, but a combination of factors could still lead to a loss of sponges on reefs. This study forms the basis for use of in vitro sponge cell models to evaluate other environmental parameters and stressors on additional sponge species.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"313-328"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of an embryonic cell line of Grapholita molesta (Lepidoptera: Tortricidae) and in vitro replication of Cydia pomonella granulovirus in it. 小圆蛾（鳞翅目：圆蛾科）胚胎细胞系的建立及卵圆蛾颗粒病毒的体外复制。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-03-01 Epub Date: 2025-04-14 DOI: 10.1007/s11626-025-01036-z

Gui-Ling Zheng, Yu-Fan Yao, Xiao-Yu Zhang, Qian-Long Yu, Jie Li, Yi-Ping Li, Dong Chu, Chang-You Li

{"title":"Establishment of an embryonic cell line of Grapholita molesta (Lepidoptera: Tortricidae) and in vitro replication of Cydia pomonella granulovirus in it.","authors":"Gui-Ling Zheng, Yu-Fan Yao, Xiao-Yu Zhang, Qian-Long Yu, Jie Li, Yi-Ping Li, Dong Chu, Chang-You Li","doi":"10.1007/s11626-025-01036-z","DOIUrl":"10.1007/s11626-025-01036-z","url":null,"abstract":"The oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae), is a major pest of fruit trees worldwide. In this study, an embryonic cell line QAU-Gm-E-L of the oriental fruit moth was successfully established. The cells grew adherently, round cells and spindle cells accounted for 43.0% and 42.2% of the total population, respectively, and rod-shaped cells accounted for 14.8%. The amplified mitochondrial cytochrome oxidase I subunit (CoI) gene fragment was 651 bp in length, and its similarity with the CoI gene of the oriental fruit moth was 100%. The chromosomes of QAU-Gm-E-L cells were granular or short rod-shaped. Its number varied from 66 to 444, indicating that aneuploidy occurred. The observations were consistent with the chromosome characteristics of lepidopteran insect cell lines. The population doubling time of QAU-Gm-E-L cells was 27.64 h. Real-time fluorescence quantitative polymerase chain reaction (qPCR) confirmed that the number of copies of Cydia pomonella granulovirus (CpGV) gradually increased in QAU-Gm-E-L cells with inoculation time. The electron microscopy observations results showed that occlusion bodies (OBs) of CpGV could be formed in the cells at 4 d post-infection; a large number of OBs were seen in the cells at 8 d post-infection. Hence, the QAU-Gm-E-L cells can support the in vitro replication and proliferation of CpGV, and it will provide an ideal material for the molecular biology research of oriental fruit moth and CpGV.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"329-336"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative genotoxicity study of agrochemicals: nuclear abnormalities, comet assay, and gene expression alterations. 农用化学品的比较遗传毒性研究：核异常、彗星试验和基因表达改变。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-03-01 Epub Date: 2025-04-22 DOI: 10.1007/s11626-025-01030-5

Ankita Salunke, Parth Pandya, Bhumi Thakkar, Pragna Parikh

{"title":"A comparative genotoxicity study of agrochemicals: nuclear abnormalities, comet assay, and gene expression alterations.","authors":"Ankita Salunke, Parth Pandya, Bhumi Thakkar, Pragna Parikh","doi":"10.1007/s11626-025-01030-5","DOIUrl":"10.1007/s11626-025-01030-5","url":null,"abstract":"Agrochemicals (AGs) are known for their ability to have a negative impact on the health of non-target species, despite the fact that they are meant to protect agricultural plants from harmful pests. Catla catla (Hamilton, 1822) gill cells (ICG) were exposed to four AGs: insecticide (Imidacloprid (IMI)), fungicide (Curzate (CZ)), herbicide (pyrazosulfuron ethyl (PE)), and fertilizer micronutrients (MN) with sublethal concentrations 1/20th, 1/10th, and 1/5th of IC50, described here as low dose (LD), medium dose (MD), and high dose (HD), respectively. A significant dose-dependent increase in the nuclear abnormalities such as micronuclei formation, bi-nucleated, and lobbed nucleated cells was observed in ICG cells treated with AGs. Of all the AGs, maximum alterations were observed with the HD of IMI followed by CZ, PE, and MN. Concurrently, the genotoxicity was determined by performing comet assays with high dose of all AGs. The gene expression of dnmt and cyp p450 were also studied through q-PCR in ICG cells. The significant increase in expression as well as alteration in cyp p450 and dnmt sequence was reported in ICG cells exposed to HD of IMI. This suggests that IMI has a genotoxic effect and may lead to epigenetic alterations.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"292-302"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of epithelial cell lines from the rainbow trout gut and gill to ammonia. 虹鳟鱼肠道和鳃上皮细胞系对氨的反应。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-03-01 Epub Date: 2025-02-05 DOI: 10.1007/s11626-024-01010-1

Daylan T Pritchard, Caio J Nicholson de Figueiroa, Niels C Bols, Lucy E J Lee

{"title":"Response of epithelial cell lines from the rainbow trout gut and gill to ammonia.","authors":"Daylan T Pritchard, Caio J Nicholson de Figueiroa, Niels C Bols, Lucy E J Lee","doi":"10.1007/s11626-024-01010-1","DOIUrl":"10.1007/s11626-024-01010-1","url":null,"abstract":"Rainbow trout epithelial cell lines from the gill, RTgill-W1, and gut, RTgutGC, were exposed to NH4Cl at 18-21 °C in L15 (basal medium) with fetal bovine serum and were found to undergo cytoplasmic vacuolization and cell death, depending on NH4Cl concentration and exposure time. Vacuolization arose within 24 h of cultures being exposed to 10-100 mM NH4Cl, and vacuoles disappeared over 24 h after NH4Cl-exposed cultures were returned to just L15/FBS. RTgill-W1 appeared more sensitive to vacuolization, with one indicator being the maximal proportion of vacuolated cells in a culture, which approached 100% in 50 mM NH4Cl for 72 h. RTgill-W1 also were more sensitive to NH4Cl-induced cell killing. For 7-d exposures, the inhibitory concentrations (IC50s) for the 50% loss of cell viability as evaluated with Alamar Blue were 30 mM NH4Cl for RTgill-W1 and 80 mM for RTgutGC. In a wound-healing assay, RTgutGC cells in 0.1 and 1 mM NH4Cl were able to migrate and cover a 500-μm gap in 5 d, like the control, but in 50 mM NH4Cl healing was blocked. In 10 mM NH4Cl, repair was slowed but by 14 d the gap was covered with cells and many of these were vacuolated. Overall, the results provide a foundation for using these two cell lines to study the physiology and toxicology of ammonia in fish.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"241-254"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of small-scale laboratory-grown cell-based fish meat from Asian seabass muscle and fin cell lines. 以亚洲海鱼肌肉和鱼鳍细胞系为原料生产小规模实验室培养的细胞鱼肉。

IF 2 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-03-01 Epub Date: 2025-04-08 DOI: 10.1007/s11626-025-01040-3

Sivaraj Mithra, Seepoo Abdul Majeed, Shaik Abdullah Eisa Abdullah, Ganesan Ajay Pathra, Gani Taju, Isaac Sarojini Bright Singh, Perumal Santhanam, Azeez Sait Sahul Hameed

{"title":"Production of small-scale laboratory-grown cell-based fish meat from Asian seabass muscle and fin cell lines.","authors":"Sivaraj Mithra, Seepoo Abdul Majeed, Shaik Abdullah Eisa Abdullah, Ganesan Ajay Pathra, Gani Taju, Isaac Sarojini Bright Singh, Perumal Santhanam, Azeez Sait Sahul Hameed","doi":"10.1007/s11626-025-01040-3","DOIUrl":"10.1007/s11626-025-01040-3","url":null,"abstract":"Aquaculture is essential to satisfying the world's increasing demand for seafood. Likewise, overfishing is becoming more common across the world, inflicting tremendous damage to the marine environment. There is a critical need for protecting sustainable fishing resources to fulfil the increasing demand for seafood. The current work focuses on the cells derived from Asian seabass muscle (SBM) and Asian seabass fin (SBF) for producing cell-based fish meat. SBM and SBF cells were seeded separately in the TubeSpin bioreactor and placed on a 3D orbital rocker. Cell sheets formed on the TubeSpin were detached and formed spheroid-like structures. These structures aggregated and formed visible tissue-like structures on 45 d of culture. Immunotyping results revealed that the presence of myosin in the cells of muscle and fin tissue, and indicating that these cells might have originated from myoblasts. The origin of cultured tissue from SBM and SBF cell lines was confirmed by amplification and sequencing of the L. calcarifer specific mitochondrial larger subunit rRNA gene. Additionally, these cells could be cultivated in multilayered forms that were appropriate for large-scale production. This approach provides a new method for the production of cell-based, laboratory-grown meat from the Asian seabass muscle and fin cell lines.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"337-344"},"PeriodicalIF":2.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0