In Vitro Cellular & Developmental Biology. Animal最新文献

miR-135a-5p exerts tumor-suppressive effects in 769P renal carcinoma cells through the TFAP2A/KRT8 signaling axis. miR-135a-5p通过TFAP2A/KRT8信号轴在769P肾癌细胞中发挥肿瘤抑制作用。

IF 1.7 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-05-08 DOI: 10.1007/s11626-026-01188-6

Delong Xie, Jizhe Zhou, Yueyue Guo, Guangyang Wan, Feiyan Lu, Yuying Guo, Sangui Yi, Zongling Liu

{"title":"miR-135a-5p exerts tumor-suppressive effects in 769P renal carcinoma cells through the TFAP2A/KRT8 signaling axis.","authors":"Delong Xie, Jizhe Zhou, Yueyue Guo, Guangyang Wan, Feiyan Lu, Yuying Guo, Sangui Yi, Zongling Liu","doi":"10.1007/s11626-026-01188-6","DOIUrl":"https://doi.org/10.1007/s11626-026-01188-6","url":null,"abstract":"The oncogenic roles of microRNAs have been extensively documented across various cancer types. In the present study, we aimed to investigate the functional impact of miR-135a-5p on the clear cell renal cell carcinoma (ccRCC) cell line 769P. Through weighted gene co-expression network analysis (WGCNA), we identified a significant association between miR-135a-5p and ccRCC progression. Furthermore, miR-135a-5p expression was downregulated in both ccRCC tissues and cell lines. Functional assays demonstrated that miR-135a-5p inhibits the progression of 769P cells. Mechanistically, miR-135a-5p directly binds to the 3' untranslated region (3'UTR) of TFAP2A, reducing its mRNA stability. In turn, TFAP2A binds to the promoter region of KRT8 and activates its transcription, thereby mediating the inhibitory effect of miR-135a-5p on 769P cell progression. In conclusion, our findings provide evidence that miR-135a-5p suppresses ccRCC progression in 769P cells through the TFAP2A/KRT8 axis, highlighting its potential as a therapeutic target for ccRCC.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isoliquiritin alleviates sepsis-induced intestinal barrier dysfunction in mice potentially by promoting autophagy. 异异黄酮可能通过促进自噬来减轻脓毒症诱导的小鼠肠道屏障功能障碍。

IF 1.7 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-05-08 DOI: 10.1007/s11626-026-01190-y

Qian Ye, Lingxuan Zhang, Xiuzhen Wang, Hong Sun, Hong Wang, Xin Wang, Junbo Zheng, Yeting Hou, Yun Wang, Yingrun Sun, Kai Liu

{"title":"Isoliquiritin alleviates sepsis-induced intestinal barrier dysfunction in mice potentially by promoting autophagy.","authors":"Qian Ye, Lingxuan Zhang, Xiuzhen Wang, Hong Sun, Hong Wang, Xin Wang, Junbo Zheng, Yeting Hou, Yun Wang, Yingrun Sun, Kai Liu","doi":"10.1007/s11626-026-01190-y","DOIUrl":"https://doi.org/10.1007/s11626-026-01190-y","url":null,"abstract":"Intestinal barrier function protection is essential to ameliorate sepsis. Isoliquiritin (ILQ) is a natural compound with broad biological activities. However, whether ILQ attenuates sepsis-induced intestinal damage remains unclear. An LPS-induced sepsis mouse model was established. ILQ inhibited body weight loss in sepsis mice. ILQ enhanced mouse intestinal function, as evidenced by decreased serum LDH levels and increased citrulline levels. Alcian blue staining showed that ILQ increased goblet cells in sepsis mouse ileum. ILQ increased the MUC2 RNA levels and decreased the claudin-2 protein expression in the sepsis mouse ileum. We subsequently examined the ILQ effects on intestinal autophagy activity. ILQ reduced the p62 protein expression and increased the conversion of LC3BI to LC3BII in sepsis mouse ileum. The LC3 protein expression was reduced in sepsis mouse ileum. An LPS-stimulated Caco-2 cell model was established. ILQ enhanced the viability of LPS-stimulated Caco-2 cells, reduced the LDH activity in cell supernatants, and increased the MUC2 RNA levels. ILQ enhanced the cellular barrier of LPS-stimulated Caco-2 cells, as indicated by the reduction in claudin-2 protein and the increase in TEER. ILQ also promoted autophagy activity in LPS-stimulated Caco-2 cells. Rescue experiments showed that an autophagy inhibitor disrupts the protective effect of ILQ on the Caco-2 cell barrier and inhibits ILQ-promoted autophagy activity. ILQ promoted the expression of LC3BII following the addition of chloroquine in LPS-stimulated Caco-2 cells. In conclusion, ILQ alleviated intestinal dysfunction and promoted autophagy activity in sepsis mice ileum, and might enhance the barrier function of LPS-stimulated Caco-2 cells via autophagy.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of Lates calcarifer primary myoblast proliferation and differentiation from direct cell isolation. 晚期钙化细胞原代成肌细胞增殖和直接细胞分离分化的特征。

IF 1.7 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-05-08 DOI: 10.1007/s11626-026-01185-9

Angela Trace, Miriam Wankell, Craig McFarlane, Lionel Hebbard

引用次数: 0

Establishment of a novel immortalized cell line derived from porcine plasmacytoid dendritic cells. 猪浆细胞样树突状细胞永生化细胞系的建立。

IF 1.7 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-05-08 DOI: 10.1007/s11626-026-01186-8

Shunichi Suzuki, Takato Takenouchi, Hirohide Uenishi

引用次数: 0

The effects of omentin on estradiol and progesterone release in human granulosa cell culture. 大网膜对人颗粒细胞培养中雌二醇和黄体酮释放的影响。

IF 1.7 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-05-08 DOI: 10.1007/s11626-026-01187-7

Pınar Tarıkahya, Burcu Baba, Leyla Özer, Tolga Ecemiş, Çağla Zübeyde Köprü

{"title":"The effects of omentin on estradiol and progesterone release in human granulosa cell culture.","authors":"Pınar Tarıkahya, Burcu Baba, Leyla Özer, Tolga Ecemiş, Çağla Zübeyde Köprü","doi":"10.1007/s11626-026-01187-7","DOIUrl":"https://doi.org/10.1007/s11626-026-01187-7","url":null,"abstract":"Adipokines serve as a link between energy metabolism and reproduction, helping to explain infertility related to polycystic ovary syndrome (PCOS). Granulosa cells are known to produce adipokines such as omentin; however, research on the potential effects of omentin on hormone levels in PCOS patients is lacking. Thus, the aim of our study was to evaluate the effect of different concentrations of omentin, FSH, and FSH + omentin on estradiol and progesterone secretion from human granulosa cells of PCOS patients. Granulosa cells were obtained from follicular fluid of 12 women undergoing IVF/ICSI treatment. Then they were treated with different doses of omentin (1 and 10 ng/ml), FSH (10 and 100 ng/ml), and with combinations of omentin and FSH for 48 h. The levels of estradiol and progesterone secreted by granulosa cells into the culture supernatants were measured by ELISA. Progesterone levels were increased when 10 ng/ml omentin was administered together with high-dose FSH (100 ng/ml), and were also increased at 1 and 10 ng/ml omentin doses compared to the control. Estradiol levels increased with 1 and 10 ng/ml omentin, and this effect was enhanced when combined with high-dose FSH; however, the combination of 1 ng/ml omentin with high-dose FSH resulted in reduced estradiol secretion. These findings demonstrated that omentin treated with different doses changed the secretion of estradiol and progesterone in granulosa cells in a dose-dependent manner.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pro-myogenic and anti-atrophic effects of Padina arborescens extract through Akt/mTOR signaling in dexamethasone-induced C2C12 cells and zebrafish model. 通过Akt/mTOR信号通路对地塞米松诱导的C2C12细胞和斑马鱼模型的促肌和抗萎缩作用。

IF 1.7 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-05-05 DOI: 10.1007/s11626-026-01181-z

Kyeong Min Lee, Wook-Chul Kim, Yun-Su Lee, Ji-Won Park, Bohyun Yun, WonWoo Lee, Kyung-Min Choi, Seung Tae Im, Seung-Hong Lee

{"title":"Pro-myogenic and anti-atrophic effects of Padina arborescens extract through Akt/mTOR signaling in dexamethasone-induced C2C12 cells and zebrafish model.","authors":"Kyeong Min Lee, Wook-Chul Kim, Yun-Su Lee, Ji-Won Park, Bohyun Yun, WonWoo Lee, Kyung-Min Choi, Seung Tae Im, Seung-Hong Lee","doi":"10.1007/s11626-026-01181-z","DOIUrl":"https://doi.org/10.1007/s11626-026-01181-z","url":null,"abstract":"Muscle atrophy, which is characterized by the loss and dysfunction of skeletal muscle proteins, is a major degenerative condition that is associated with aging and glucocorticoid therapy. Marine-derived compounds, particularly polyphenols, have recently potential in modulating muscle metabolism and regeneration. This study aimed to investigate the effects of the ethanolic extract from Padina arborescens (PAE) on myogenic differentiation and dexamethasone (DEXA)-induced muscle atrophy using C2C12 myotubes and a zebrafish model. PAE treatment significantly promoted myotube differentiation by modulating the Akt/mTOR signaling pathway and enhancing the expression level of myogenic regulatory factors (MyoD and myogenin). In DEXA-treated myotubes, PAE effectively suppressed the ubiquitin proteasome system, restored myosin heavy chain protein synthesis, and recovered myotube morphology. In vivo, PAE supplementation ameliorated the DEXA-induced locomotor dysfunction in zebrafish without causing developmental or neurotoxic abnormalities, as confirmed by the normal survival rate, body length, and heart rate. Collectively, these findings indicated that polyphenol-rich PAE exerts protective and anabolic effects by promoting myogenesis and preventing glucocorticoid-induced muscle degradation. Therefore, PAE may be used as a promising marine-derived therapeutic agent for maintaining skeletal muscle health and preventing muscle atrophy.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and characterization of air-liquid interface cultures of human small airway epithelial cells from two commercial sources. 两种商业来源的人小气道上皮细胞气液界面培养的建立和表征。

IF 1.7 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-04-28 DOI: 10.1007/s11626-026-01180-0

Kyoung Jin Nho

引用次数: 0

RYK silencing-modified bone marrow-derived mesenchymal stem cells suppress gastric cancer progression. RYK沉默修饰的骨髓间充质干细胞抑制胃癌进展。

IF 1.7 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-04-10 DOI: 10.1007/s11626-026-01175-x

Yongan Fu, Zongda Cai, Yangqiang Wang, Mingjin Huang, Jinghua Huang

{"title":"RYK silencing-modified bone marrow-derived mesenchymal stem cells suppress gastric cancer progression.","authors":"Yongan Fu, Zongda Cai, Yangqiang Wang, Mingjin Huang, Jinghua Huang","doi":"10.1007/s11626-026-01175-x","DOIUrl":"https://doi.org/10.1007/s11626-026-01175-x","url":null,"abstract":"Gastric cancer (GC) is a prevalent malignant tumor threatening human health. This study aimed to explore the potential mechanism of receptor-like tyrosine kinase (RYK) silencing-modified bone marrow-derived mesenchymal stem cells (BMSCs) in the progression of GC. BMSCs were transfected with the RYK siRNA and negative controls. Cell co-culture experiments were used to explore the interaction between different BMSCs and human gastric carcinoma cell line NCI-N87. Cancer cell proliferation, cell cycle, apoptosis, colony formative ability, and invasive ability were assessed. Western blot analysis was performed to determine the protein levels of cyclinA, Bcl-xL, Bcl-2, cleaved caspase 3, cleaved caspase 7, cleaved caspase 9, and cleaved PARP1 in NCI-N87 cells. Compared with NCI-N87 cells, co-culture of si-NC-modified BMSCs with NCI-N87 cells promoted the proliferation, colony formative ability, and invasion of NCI-N87 cells, inhibited the apoptosis of NCI-N87 cells, and reduced the proportion of NCI-N87 cells present in G2/M phase cells. In addition, compared with the si-NC-BMSCs + N87 group, the si-RYK-BMSCs + N87 group inhibited the proliferation, colony formative ability, and invasion of NCI-N87 cells, promoted the apoptosis of NCI-N87 cells, and increased the proportion of G2/M phase cells. Thus, RYK silencing-modified BMSCs can inhibit the proliferation, colony formative ability, and invasion of NCI-N87 cells, while inducing apoptosis and G2/M phase arrest.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147654039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated assessment of graphene oxide and graphitic carbon nitride nanoparticles: from synthesis to in vitro biological impact on zebrafish (Danio rerio) cell models. 氧化石墨烯和氮化碳纳米颗粒的综合评估：从合成到对斑马鱼细胞模型的体外生物学影响。

IF 1.7 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-04-08 DOI: 10.1007/s11626-026-01178-8

Baskaran Valarmathi, Sivaraj Mithra, Seepoo Abdul Majeed, Kannan Lathika Dharshini, Valiyathur Mohammed Furqaan, Katiyan Mohammed Rehan, Gani Taju, Sakvai Mohammed Safiullah, Kottur Anwar Basha, Sathar Zaheer Ahmed, Amtuz Zehra, Azeez Sait Sahul Hameed

{"title":"Integrated assessment of graphene oxide and graphitic carbon nitride nanoparticles: from synthesis to in vitro biological impact on zebrafish (Danio rerio) cell models.","authors":"Baskaran Valarmathi, Sivaraj Mithra, Seepoo Abdul Majeed, Kannan Lathika Dharshini, Valiyathur Mohammed Furqaan, Katiyan Mohammed Rehan, Gani Taju, Sakvai Mohammed Safiullah, Kottur Anwar Basha, Sathar Zaheer Ahmed, Amtuz Zehra, Azeez Sait Sahul Hameed","doi":"10.1007/s11626-026-01178-8","DOIUrl":"https://doi.org/10.1007/s11626-026-01178-8","url":null,"abstract":"Graphene and its derivatives have been increasingly explored for various biomedical applications. Despite their promising potential, the accelerated development and integration of these materials in medical technologies have raised important questions regarding their biocompatibility, toxicity, and overall safety within physiological systems. Graphene oxide (GO) and graphitic carbon nitride (g-C3N4) nanoparticles are widely studied for their strong adsorption and photocatalytic properties, making them effective materials for removing emerging contaminants from water and wastewater. In this study, we performed a detailed evaluation of the acute (24 h) cytotoxic effects of GO and g-C3N4 nanoparticles on DrG and DrF cells by assessing cell morphology, viability, mortality, and membrane integrity. GO exhibited minimal cytotoxicity under the tested conditions, with only a slight reduction in cell viability observed at 100 µg/mL concentrations due to dose-dependent oxidative stress. In contrast, g-C3N4 nanoparticles demonstrated significant cytotoxicity in both cell types, even at 40 µg/mL concentrations, inducing oxidative stress, elevated ROS production, mitochondrial dysfunction, and a marked decrease in cell viability. These findings underscore the importance of dose consideration in the design of GO-based biomedical applications and highlight the potential limitations of g-C3N4 in similar contexts. Overall, GO showed good biocompatibility, further supported by the favorable growth of cells on GO-coated substrates (50 µg/mL).","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147638728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cabozantinib activates TFEB-mediated autophagy to exert anti-tumor effects in hepatocellular carcinoma. 卡博赞替尼激活tfeb介导的自噬，在肝细胞癌中发挥抗肿瘤作用。

IF 1.7 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2026-04-08 DOI: 10.1007/s11626-026-01161-3

Cheng Chen, Yongjie Hu, Chenggang Zhang, Xiaocui Zhou

{"title":"Cabozantinib activates TFEB-mediated autophagy to exert anti-tumor effects in hepatocellular carcinoma.","authors":"Cheng Chen, Yongjie Hu, Chenggang Zhang, Xiaocui Zhou","doi":"10.1007/s11626-026-01161-3","DOIUrl":"https://doi.org/10.1007/s11626-026-01161-3","url":null,"abstract":"Autophagy signaling plays a crucial yet complex role in hepatocellular carcinoma (HCC), influencing tumor progression and treatment response. Cabozantinib is an orally administered multi-kinase inhibitor used in the treatment of various advanced cancers, including renal cell carcinoma and HCC. However, its precise mechanisms of action in HCC require further elucidation. Our study first revealed elevated levels of phosphorylated TFEB (p-TFEB, Ser142) in human HCC tissues and cell lines, indicating impaired TFEB-mediated autophagic flux in hepatocellular carcinoma. Treatment with Cabozantinib induced dose-dependent cytotoxicity, oxidative stress (increased ROS and decreased GSH), and mitochondrial dysfunction (reduced Complex IV activity and ATP production). Furthermore, Cabozantinib promoted autophagy activation, as evidenced by increased autophagosome formation, elevated LC3-II/I conversion and Beclin1 expression, and decreased p62 levels. Mechanistically, Cabozantinib inhibited TFEB phosphorylation at Ser142 and enhanced its nuclear translocation. Critically, TFEB knockdown abolished Cabozantinib-induced autophagy, confirming that the pro-autophagic effects are TFEB-dependent. These findings demonstrate that Cabozantinib exerts its anti-tumor activity in HCC through activating TFEB-mediated autophagy pathway by inducing oxidative stress and mitochondrial damage.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147638769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0