In Vitro Cellular & Developmental Biology. Animal最新文献_第2页

Development and characterization of red seabream (Pagrus major) muscle satellite cell lines for cultivated seafood: highlighting serum reduction and microcarrier expansion. 养殖海产品用红鲷（Pagrus major）肌肉卫星细胞系的发育和特性：突出血清还原和微载体扩增。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2025-05-23 DOI: 10.1007/s11626-025-01045-y

Selvakumari Ulagesan, Sathish Krishnan, Taek-Jeong Nam, Youn-Hee Choi

{"title":"Development and characterization of red seabream (Pagrus major) muscle satellite cell lines for cultivated seafood: highlighting serum reduction and microcarrier expansion.","authors":"Selvakumari Ulagesan, Sathish Krishnan, Taek-Jeong Nam, Youn-Hee Choi","doi":"10.1007/s11626-025-01045-y","DOIUrl":"https://doi.org/10.1007/s11626-025-01045-y","url":null,"abstract":"Cellular aquaculture requires well-characterized marine cell lines for sustainable seafood production. This study developed muscle satellite cell lines from red seabream (Pagrus major) (PMMSC) to support cultured seafood production. Primary cells, isolated via collagenase digestion (2.61 × 105 cells/mL), were cultured in L- 15 medium under varying FBS levels (10-20%) and temperatures (20-28 °C). Optimal growth occurred at 24 °C with 10% FBS, forming dense monolayers by day 9. Differentiation into myotubes, induced with 2% horse serum, was confirmed by myogenic protein expression (Pax7, MYH, MyoD, MyoG, Desmin) over 12 d. PMMSC proliferation was evaluated under reduced-serum conditions supplemented with Spirulina platensis extract, an algae-derived alternative to fetal bovine serum. The algae extract improved cell proliferation, demonstrating its potential for sustainable seafood production. Additionally, 3D cell expansion using gelatin microcarriers in a bioreactor resulted in an 8.18-fold increase in cell count, showcasing its potential for scalable production. This research provides a valuable resource for the cellular aquaculture field by establishing a well-characterized red seabream muscle satellite cell line and demonstrating the potential of algae-based serum replacements.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An in vitro cellular model for measuring the impact of thermal stress on Florida reef sponges. 用于测量热应力对佛罗里达礁海绵影响的体外细胞模型。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2025-05-22 DOI: 10.1007/s11626-025-01034-1

Megan Conkling, Tobin Hindle, Zhixiao Xie, Weibo Liu, Timothy Moore, Shirley A Pomponi

{"title":"An in vitro cellular model for measuring the impact of thermal stress on Florida reef sponges.","authors":"Megan Conkling, Tobin Hindle, Zhixiao Xie, Weibo Liu, Timothy Moore, Shirley A Pomponi","doi":"10.1007/s11626-025-01034-1","DOIUrl":"https://doi.org/10.1007/s11626-025-01034-1","url":null,"abstract":"Coral reefs are threatened by recurrent mortality incidents in their native habitats brought on by natural and anthropogenic stressors. Elevated temperature has been indicated as a major causing factor. Although ongoing research is focused on corals, sponges are an important benthic organism on coral reefs and are often overlooked. An accurate and standardized method is needed to determine the environmental limits and thresholds of sponges commonly found on coral reefs. We established an in vitro sponge cell model and evaluated the effect of elevated temperatures on primary cell cultures of five common Florida reef sponges-Agelas clathrodes, Aplysina fulva, Cliona varians, Geodia neptuni, and Xestospongia muta. Analysis of the results revealed that the impact of increased temperatures had no significant effect at the cellular level, but there are changes at the molecular level. Shifts in the sponges' transcriptomic profiles induced by increased temperatures, trigger processes related to signal transduction, apoptosis, and cell repair pathways. Further elevation of temperature corresponding to local extremes activated the immune response and programmed cell death. The results of the present study are based on both cellular and molecular data obtained from the in vitro cell model which highlight the minimal response of all five species to thermal stress, providing an insight into the mechanisms involved in the adaptive process. Furthermore, they suggest a resilience of these sponges to the current thermal extremes, but a combination of factors could still lead to a loss of sponges on reefs. This study forms the basis for use of in vitro sponge cell models to evaluate other environmental parameters and stressors on additional sponge species.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

mTORC1 signalling and protein synthesis are elevated in response to amino acids in human myotubes obtained from young, old, and old trained men. mTORC1信号和蛋白质合成在年轻人、老年人和老年受过训练的人的肌管中对氨基酸的反应中升高。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2025-05-20 DOI: 10.1007/s11626-025-01041-2

Stephanie D Gagnon, Jiani Qian, Vladimir Belhac, Neil R W Martin

{"title":"mTORC1 signalling and protein synthesis are elevated in response to amino acids in human myotubes obtained from young, old, and old trained men.","authors":"Stephanie D Gagnon, Jiani Qian, Vladimir Belhac, Neil R W Martin","doi":"10.1007/s11626-025-01041-2","DOIUrl":"https://doi.org/10.1007/s11626-025-01041-2","url":null,"abstract":"Ageing and reduced levels of physical activity are associated with desensitisation of skeletal muscle to the anabolic effects of amino acids. In vitro studies have indicated that many properties of skeletal muscle tissue are retained in human myotubes, including metabolic alterations associated with exercise and disease. However, the interaction between ageing and physical activity on amino acid sensing and growth has not been explored in human myotubes in vitro. Muscle-derived cells were isolated from biopsies taken from eight young (Y: 23.4 ± 1.9 yr), six older (O: 72.5 ± 5.0 yr), and nine older exercise trained (OT: 71.0 ± 4.1 yr, n = 9) men, and myotube cultures were generated and investigated for growth parameters and amino acid induced changes in mTORC1 signalling and protein synthesis. Our results indicated that muscle cell fusion was similar between groups, but myotube diameter was lower in cultures derived from O individuals. Despite this, mTORC1 signalling, as indicated by immunoblots for phosphorylation of mTORSer2448, rpS6Ser235/236, and 4E-BP1Thr37/46 increased to a similar extent in response to amino acid availability in Y, O, and OT myotubes. Furthermore, measures of protein synthesis using the SUnSET assay were increased similarly between groups after the addition of amino acids. These data suggest that skeletal muscle desensitisation to amino acids with ageing is not observed in myotubes cultured in vitro, which could be reflective of the healthy individuals tested in our study or point towards the importance of the muscle niche in the impairments in muscle metabolism in ageing.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced myogenic differentiation capacity of satellite cell-derived myoblasts in male ICR mice compared with male C57BL/6 and BALB/c mice. 雄性ICR小鼠与雄性C57BL/6和BALB/c小鼠相比，卫星细胞来源的成肌细胞的成肌分化能力降低。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2025-05-19 DOI: 10.1007/s11626-025-01035-0

Takahiro Suzuki, Yuriko Nishi, Taku Koyama, Minori Nakada, Rio Arimatsu, Yusuke Komiya, Aoi Ogawa, Rika Osaki, Takahiro Maeno, Ai Saiga Egusa, Mako Nakamura, Ryuichi Tatsumi, Koichi Ojima, Takanori Nishimura

{"title":"Reduced myogenic differentiation capacity of satellite cell-derived myoblasts in male ICR mice compared with male C57BL/6 and BALB/c mice.","authors":"Takahiro Suzuki, Yuriko Nishi, Taku Koyama, Minori Nakada, Rio Arimatsu, Yusuke Komiya, Aoi Ogawa, Rika Osaki, Takahiro Maeno, Ai Saiga Egusa, Mako Nakamura, Ryuichi Tatsumi, Koichi Ojima, Takanori Nishimura","doi":"10.1007/s11626-025-01035-0","DOIUrl":"https://doi.org/10.1007/s11626-025-01035-0","url":null,"abstract":"Many strains of wild-type laboratory mice have been developed for studies in the life sciences, including skeletal muscle cell biology. Muscle regeneration capacity differs among wild-type mouse strains. However, few studies have focused on whether myogenic stem cells (satellite cells) are directly related to mouse strain-dependent myoregeneration gaps using in vitro culture models. In this study, we selected three major wild-type mouse strains, CD1 (outbred; Jcl:ICR [ICR]), C57BL/6NJcl (inbred; B6), and BALB/cAJcl (inbred; C), which are widely used in laboratory experiments. Initially, we compared myotube fusion capabilities using satellite cell-derived myoblasts. The results showed that cell cultures isolated from male ICR mice could not efficiently form myotubes owing to low expression levels of myogenic regulatory factors (e.g., MyoD, myogenin, myocyte enhancer factor [MEF] 2A, and MEF2C) compared with B6 and C mouse strains. Next, we compared the myofiber-type compositions of muscle tissues and cultured myotubes among male mice from each of the three strains. Although each muscle tissue used for satellite cell isolation similarly expressed fast-twitch myofiber markers in all mouse strains, male ICR-derived myoblasts formed abundant amounts of slow-type myotubes. By contrast, myotubes from male B6 and C mice expressed substantial levels of fast-twitch myofiber markers. We also performed a comparative experiment in female ICR, B6, and C mouse strains, similar to the male mouse experiments. The myogenic differentiation potencies of myoblasts and myofiber-type compositions of myotubes in female mouse strains were similar. Thus, male ICR-derived satellite cells (myoblasts) had low myogenic differentiation potential, which may be associated with the tendency slow-twitch myotube formation.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryopreservation of biological materials: applications and economic perspectives. 生物材料的低温保存：应用和经济前景。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2025-04-23 DOI: 10.1007/s11626-025-01027-0

Suja Aarattuthodi, David Kang, Sanjay Kumar Gupta, Paula Chen, Bethany Redel, Moureen Matuha, Haitham Mohammed, Amit Kumar Sinha

{"title":"Cryopreservation of biological materials: applications and economic perspectives.","authors":"Suja Aarattuthodi, David Kang, Sanjay Kumar Gupta, Paula Chen, Bethany Redel, Moureen Matuha, Haitham Mohammed, Amit Kumar Sinha","doi":"10.1007/s11626-025-01027-0","DOIUrl":"https://doi.org/10.1007/s11626-025-01027-0","url":null,"abstract":"Cryopreservation is a transformative technology that allows for the long-term storage of biological materials by cooling them to extremely low temperatures at which metabolic and biochemical processes are effectively slowed or halted. Cryopreservation utilizes various techniques to minimize ice crystal formation and cellular damage during freezing and thawing processes. This technology has broad applications in the fields of medicine, agriculture, and conservation, spanning across stem cell research, reproductive and regenerative medicine, organ transplantation, and cell-based therapies, each with significant economic implications. While current techniques and their associated costs present certain challenges, ongoing research advancements related to cryoprotectants, cooling methods, and automation promise to enhance efficiency and accessibility, potentially broadening the technology's impact across various sectors. This review focuses on the applications of cryopreservation, research advancements, and economic implications, emphasizing the importance of continued research to overcome the current limitations.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative genotoxicity study of agrochemicals: nuclear abnormalities, comet assay, and gene expression alterations. 农用化学品的比较遗传毒性研究：核异常、彗星试验和基因表达改变。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2025-04-22 DOI: 10.1007/s11626-025-01030-5

Ankita Salunke, Parth Pandya, Bhumi Thakkar, Pragna Parikh

{"title":"A comparative genotoxicity study of agrochemicals: nuclear abnormalities, comet assay, and gene expression alterations.","authors":"Ankita Salunke, Parth Pandya, Bhumi Thakkar, Pragna Parikh","doi":"10.1007/s11626-025-01030-5","DOIUrl":"https://doi.org/10.1007/s11626-025-01030-5","url":null,"abstract":"Agrochemicals (AGs) are known for their ability to have a negative impact on the health of non-target species, despite the fact that they are meant to protect agricultural plants from harmful pests. Catla catla (Hamilton, 1822) gill cells (ICG) were exposed to four AGs: insecticide (Imidacloprid (IMI)), fungicide (Curzate (CZ)), herbicide (pyrazosulfuron ethyl (PE)), and fertilizer micronutrients (MN) with sublethal concentrations 1/20th, 1/10th, and 1/5th of IC50, described here as low dose (LD), medium dose (MD), and high dose (HD), respectively. A significant dose-dependent increase in the nuclear abnormalities such as micronuclei formation, bi-nucleated, and lobbed nucleated cells was observed in ICG cells treated with AGs. Of all the AGs, maximum alterations were observed with the HD of IMI followed by CZ, PE, and MN. Concurrently, the genotoxicity was determined by performing comet assays with high dose of all AGs. The gene expression of dnmt and cyp p450 were also studied through q-PCR in ICG cells. The significant increase in expression as well as alteration in cyp p450 and dnmt sequence was reported in ICG cells exposed to HD of IMI. This suggests that IMI has a genotoxic effect and may lead to epigenetic alterations.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of an embryonic cell line of Grapholita molesta (Lepidoptera: Tortricidae) and in vitro replication of Cydia pomonella granulovirus in it. 小圆蛾（鳞翅目：圆蛾科）胚胎细胞系的建立及卵圆蛾颗粒病毒的体外复制。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2025-04-14 DOI: 10.1007/s11626-025-01036-z

Gui-Ling Zheng, Yu-Fan Yao, Xiao-Yu Zhang, Qian-Long Yu, Jie Li, Yi-Ping Li, Dong Chu, Chang-You Li

{"title":"Establishment of an embryonic cell line of Grapholita molesta (Lepidoptera: Tortricidae) and in vitro replication of Cydia pomonella granulovirus in it.","authors":"Gui-Ling Zheng, Yu-Fan Yao, Xiao-Yu Zhang, Qian-Long Yu, Jie Li, Yi-Ping Li, Dong Chu, Chang-You Li","doi":"10.1007/s11626-025-01036-z","DOIUrl":"https://doi.org/10.1007/s11626-025-01036-z","url":null,"abstract":"The oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae), is a major pest of fruit trees worldwide. In this study, an embryonic cell line QAU-Gm-E-L of the oriental fruit moth was successfully established. The cells grew adherently, round cells and spindle cells accounted for 43.0% and 42.2% of the total population, respectively, and rod-shaped cells accounted for 14.8%. The amplified mitochondrial cytochrome oxidase I subunit (CoI) gene fragment was 651 bp in length, and its similarity with the CoI gene of the oriental fruit moth was 100%. The chromosomes of QAU-Gm-E-L cells were granular or short rod-shaped. Its number varied from 66 to 444, indicating that aneuploidy occurred. The observations were consistent with the chromosome characteristics of lepidopteran insect cell lines. The population doubling time of QAU-Gm-E-L cells was 27.64 h. Real-time fluorescence quantitative polymerase chain reaction (qPCR) confirmed that the number of copies of Cydia pomonella granulovirus (CpGV) gradually increased in QAU-Gm-E-L cells with inoculation time. The electron microscopy observations results showed that occlusion bodies (OBs) of CpGV could be formed in the cells at 4 d post-infection; a large number of OBs were seen in the cells at 8 d post-infection. Hence, the QAU-Gm-E-L cells can support the in vitro replication and proliferation of CpGV, and it will provide an ideal material for the molecular biology research of oriental fruit moth and CpGV.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improve your success with fish cell lines-small things that matter. 提高鱼细胞系的成功率——小事情很重要。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2025-04-09 DOI: 10.1007/s11626-025-01042-1

Anita Solhaug, Georgina C Dowd, Vivian R Dayeh, Hilde Sindre, Lucy E J Lee, Niels C Bols

引用次数: 0

Production of small-scale laboratory-grown cell-based fish meat from Asian seabass muscle and fin cell lines. 以亚洲海鱼肌肉和鱼鳍细胞系为原料生产小规模实验室培养的细胞鱼肉。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2025-04-08 DOI: 10.1007/s11626-025-01040-3

Sivaraj Mithra, Seepoo Abdul Majeed, Shaik Abdullah Eisa Abdullah, Ganesan Ajay Pathra, Gani Taju, Isaac Sarojini Bright Singh, Perumal Santhanam, Azeez Sait Sahul Hameed

{"title":"Production of small-scale laboratory-grown cell-based fish meat from Asian seabass muscle and fin cell lines.","authors":"Sivaraj Mithra, Seepoo Abdul Majeed, Shaik Abdullah Eisa Abdullah, Ganesan Ajay Pathra, Gani Taju, Isaac Sarojini Bright Singh, Perumal Santhanam, Azeez Sait Sahul Hameed","doi":"10.1007/s11626-025-01040-3","DOIUrl":"https://doi.org/10.1007/s11626-025-01040-3","url":null,"abstract":"Aquaculture is essential to satisfying the world's increasing demand for seafood. Likewise, overfishing is becoming more common across the world, inflicting tremendous damage to the marine environment. There is a critical need for protecting sustainable fishing resources to fulfil the increasing demand for seafood. The current work focuses on the cells derived from Asian seabass muscle (SBM) and Asian seabass fin (SBF) for producing cell-based fish meat. SBM and SBF cells were seeded separately in the TubeSpin bioreactor and placed on a 3D orbital rocker. Cell sheets formed on the TubeSpin were detached and formed spheroid-like structures. These structures aggregated and formed visible tissue-like structures on 45 d of culture. Immunotyping results revealed that the presence of myosin in the cells of muscle and fin tissue, and indicating that these cells might have originated from myoblasts. The origin of cultured tissue from SBM and SBF cell lines was confirmed by amplification and sequencing of the L. calcarifer specific mitochondrial larger subunit rRNA gene. Additionally, these cells could be cultivated in multilayered forms that were appropriate for large-scale production. This approach provides a new method for the production of cell-based, laboratory-grown meat from the Asian seabass muscle and fin cell lines.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of 27 cell lines derived from various insects. 建立27个来自不同昆虫的细胞系。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2025-04-08 DOI: 10.1007/s11626-025-01031-4

Kazuyo Watanabe, Shigeo Imanishi, Takumi Kayukawa, Ken Tateishi

引用次数: 0