In Vitro Cellular & Developmental Biology. Animal最新文献_第9页

MiR-483-3p promotes dental pulp stem cells osteogenic differentiation via the MAPK signaling pathway by targeting ARRB2. MiR-483-3p 通过靶向 ARRB2，通过 MAPK 信号通路促进牙髓干细胞成骨分化。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2024-09-01 Epub Date: 2024-06-04 DOI: 10.1007/s11626-024-00929-9

Xin Yu, Juan Ge, Huimin Xie, Jialu Qian, Wenqian Xia, Qinghua Wang, Xiaorong Zhou, Yan Zhou

{"title":"MiR-483-3p promotes dental pulp stem cells osteogenic differentiation via the MAPK signaling pathway by targeting ARRB2.","authors":"Xin Yu, Juan Ge, Huimin Xie, Jialu Qian, Wenqian Xia, Qinghua Wang, Xiaorong Zhou, Yan Zhou","doi":"10.1007/s11626-024-00929-9","DOIUrl":"10.1007/s11626-024-00929-9","url":null,"abstract":"Human dental pulp stem cells (DPSCs) have become an important component for bone tissue engineering and regenerative medicine due to their ability to differentiate into osteoblast precursors. Two miRNA chip datasets (GSE138180 and E-MTAB-3077) of DPSCs osteogenic differentiation were analyzed respectively to find the expression of miR-483-3p significantly increased in the differentiated groups. We further confirmed that miR-483-3p continued to overexpress during osteogenic differentiation of DPSCs, especially reaching its peak on the 7th day. Moreover, miR-483-3p could significantly promote the expression of osteogenic markers including RUNX2 and OSX, and activate MAPK signaling pathway by inducing phosphorylation of ERK, p38, and JNK. In addition, as a significant gene within the MAPK signaling pathway, ARRB2 was identified as the target gene of miR-483-3p by bioinformatic prediction and experimental verification. In conclusion, we identified miR-483-3p could promote osteogenic differentiation of DPSCs via the MAPK signaling pathway by targeting ARRB2.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"879-887"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141236927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Education Posters. 教育海报。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2024-09-01 DOI: 10.1007/s11626-024-00965-5

引用次数: 0

Development and characterization of primary cell culture from the spinal cord of Asian seabass, Lates calcarifer. 亚洲鲈鱼脊髓原代细胞培养的发展和特征。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2024-09-01 Epub Date: 2024-08-05 DOI: 10.1007/s11626-024-00938-8

Sivaraj Mithra, Seepoo Abdul Majeed, Gani Taju, Sugumar Vimal, Azeez Sait Sahul Hameed

{"title":"Development and characterization of primary cell culture from the spinal cord of Asian seabass, Lates calcarifer.","authors":"Sivaraj Mithra, Seepoo Abdul Majeed, Gani Taju, Sugumar Vimal, Azeez Sait Sahul Hameed","doi":"10.1007/s11626-024-00938-8","DOIUrl":"10.1007/s11626-024-00938-8","url":null,"abstract":"Asian seabass, Lates calcarifer, is one of the most important fish species in aquaculture. An attempt was made to develop a primary cell culture from the spinal cord of Lates calcarifer by the enzymatic and mechanical dissociation method. The primary cell culture was sub-cultured for 20 times in Leibovitz's L-15 medium with 20% fetal bovine serum (FBS) and 0.5 nM of human neurotrophin-3 at 28°C. The primary cell culture was cryopreserved at different passage levels and recovery of cells after long-term storage was estimated about 75-85%. The authenticity of origin of primary cell culture from L. calcarifer was confirmed by polymerase chain reaction assay using species-specific mitochondrial 12S rRNA primer. The primary cell culture was designated as seabass spinal cord cells (SBSC). The cells morphologically resembled the neurons due to their neural-like prolongations and star-like structure. Immunophenotypic analysis of the SBSC revealed that they are of neuronal origin. The SBSC were found to be highly susceptible to striped jack nervous necrosis virus (SJNNV) and infection in the cells was confirmed by RT-PCR. In conclusion, this is the first innovative euryhaline fish neuronal primary cell culture of L. calcarifer now available for neurophysiological and neurotoxicological studies.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"825-831"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of 3D gingival in vitro models using primary gingival cells. 利用原代牙龈细胞开发三维牙龈体外模型。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2024-09-01 Epub Date: 2024-06-18 DOI: 10.1007/s11626-024-00923-1

Christelle Plaza, Christophe Capallere, Celine Meyrignac, Marianne Arcioni, Isabelle Imbert

引用次数: 0

Exploring the osteogenic potential of semisynthetic triterpenes from Combretum leprosum: An in vitro and in silico study. 探索麻风树半合成三萜类化合物的成骨潜力：体外和硅学研究。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2024-09-01 Epub Date: 2024-07-11 DOI: 10.1007/s11626-024-00928-w

Valdo Nogueira-Júnior, Fátima Regina N Sousa, Conceição da S M Rebouças, Helyson L B Braz, Maria Luana G Dos S Morais, Paula Goes, Gerly Anne de C Brito, Roberta Jeane B Jorge, Francisco Geraldo Barbosa, Jair Mafezoli, Carlos José A Silva-Filho, André Luiz de O Capistrano, Mirna M Bezerra, Renata F de C Leitão

{"title":"Exploring the osteogenic potential of semisynthetic triterpenes from Combretum leprosum: An in vitro and in silico study.","authors":"Valdo Nogueira-Júnior, Fátima Regina N Sousa, Conceição da S M Rebouças, Helyson L B Braz, Maria Luana G Dos S Morais, Paula Goes, Gerly Anne de C Brito, Roberta Jeane B Jorge, Francisco Geraldo Barbosa, Jair Mafezoli, Carlos José A Silva-Filho, André Luiz de O Capistrano, Mirna M Bezerra, Renata F de C Leitão","doi":"10.1007/s11626-024-00928-w","DOIUrl":"10.1007/s11626-024-00928-w","url":null,"abstract":"Combretum leprosum Mart. is a plant of the Combretaceae family, widely distributed in the Northeast region of Brazil, popularly used as an anti-inflammatory agent, and rich in triterpenes. This study evaluated in vitro and in silico potential osteogenic of two semisynthetic triterpenes (CL-P2 and CL-P2A) obtained from the pentacyclic triterpene 3β,6β,16β-trihydroxylup-20(29)-ene (CL-1) isolated from C. leprosum. Assays were carried out in cultured murine osteoblasts (OFCOL II), first investigating the possible toxicity of the compounds on these cells through viability assays (MTT). Cell proliferation and activation were investigated by immunohistochemical evaluation of Ki-67, bone alkaline phosphatase (ALP) activity, and mineralization test by Von Kossa. Molecular docking analysis was performed to predict the binding affinity of CL-P2 and CL-P2A to target proteins involved in the regulation of osteogenesis, including: bone morphogenetic protein 2 (BMP-2), proteins related to Wingless-related integration (WNT) pathway (Low-density lipoprotein receptor-related protein 6-LRP6 and sclerostin-SOST), and receptor activator of nuclear factor (NF)-kB-ligand (RANK-L). Next, Western Blot and immunofluorescence investigated BMP-2, WNT, RANK-L, and OPG protein expressions in cultured murine osteoblasts (OFCOL II). None of the CL-P2 and CL-P2A concentrations were toxic to osteoblasts. Increased cell proliferation, ALP activity, and bone mineralization were observed. Molecular docking assays demonstrated interactions with BMP-2, LRP6, SOST, and RANK-L/OPG. There was observed increased expression of BMP-2, WNT, and RANK-L/OPG proteins. These results suggest, for the first time, the osteogenic potential of CL-P2 and CL-P2A.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"853-867"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

16th International Conference on Invertebrate and Fish Cell Culture. 第16届无脊椎动物和鱼类细胞培养国际会议。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2024-09-01 DOI: 10.1007/s11626-024-00951-x

引用次数: 0

Atomic force microscopy characterization of white and beige adipocyte differentiation. 原子力显微镜表征白色和米色脂肪细胞的分化。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2024-09-01 Epub Date: 2024-06-04 DOI: 10.1007/s11626-024-00925-z

Alia Mallah, Katerina Stojkova, Ronald N Cohen, Nehal Abu-Lail, Eric M Brey, Maria A Gonzalez Porras

{"title":"Atomic force microscopy characterization of white and beige adipocyte differentiation.","authors":"Alia Mallah, Katerina Stojkova, Ronald N Cohen, Nehal Abu-Lail, Eric M Brey, Maria A Gonzalez Porras","doi":"10.1007/s11626-024-00925-z","DOIUrl":"10.1007/s11626-024-00925-z","url":null,"abstract":"Adipose tissue plays an essential role in systemic metabolism with white adipose tissue (WAT) making up most of the tissue and being involved in the regulation of energy homeostasis, and brown and beige adipose tissue (BAT) exhibiting thermogenic activity. There is promise in the conversion of white adipocytes into beige ones as a therapeutic potential to control and enhance systemic metabolism, but it is difficult to maintain this transformation in vivo because we do not fully understand the mechanism of conversion. In this study, we applied atomic force microscopy (AFM) to characterize beige or white adipocytes during the process of differentiation for morphology, roughness, adhesion, and elasticity at different time points. As cells differentiated to white and beige adipocytes, they exhibited morphological changes as they lipid loaded, transitioning from flattened elongated cells to a rounded shape indicating adipogenesis. While there was an initial decrease in elasticity for both beige and white adipocytes, white adipocytes exhibited a higher elasticity than beige adipocytes at all time points. Beige and white adipogenesis exhibited a decrease in adhesion energy compared to preadipocytes, yet at day 12, white adipocytes had a significant increase in adhesion energy compared to beige adipocytes. This work shows significant differences in the mechanical properties of white vs. beige adipocytes during differentiation. Results from this study contribute to a better understanding of the differentiation of adipocytes which are vital to the therapeutic induction, engineered models, and maintenance of beige adipocytes as a potential approach for enhancing systemic metabolism.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"842-852"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141236773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated bioinformatics and validation reveal PTGS2 and its related molecules to alleviate TNF-α-induced endothelial senescence. 综合生物信息学和验证揭示了 PTGS2 及其相关分子可缓解 TNF-α 诱导的内皮衰老。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2024-09-01 Epub Date: 2024-06-10 DOI: 10.1007/s11626-024-00931-1

Hongjie Xu, He Wang, Xiaoping Ning, Zhiyun Xu, Guanxin Zhang

{"title":"Integrated bioinformatics and validation reveal PTGS2 and its related molecules to alleviate TNF-α-induced endothelial senescence.","authors":"Hongjie Xu, He Wang, Xiaoping Ning, Zhiyun Xu, Guanxin Zhang","doi":"10.1007/s11626-024-00931-1","DOIUrl":"10.1007/s11626-024-00931-1","url":null,"abstract":"Accumulative evidences have indicated the interaction between cellular senescence and ferroptosis. This study intends to investigate the ferroptosis-related molecular markers in TNF-α-induced endothelial senescence. The microarray expression dataset (GSE195517) was used to identify the differently expressed ferroptosis-related genes (DEFRGs) through weighted gene co-expressed network analysis (WGCNA). GO and KEGG were performed to explore the biological function. Furthermore, hub genes were identified after protein-protein interaction (PPI) analysis and validated through real-time qPCR (RT-qPCR). Then, a drug-gene network was established to predict potential drugs for the hub genes. Seven DEFRGs were recognized in the TNF-α-induced HUVEC senescence. Moreover, four hub genes (PTGS2, TNFAIP3, CXCL2, and IL6 are upregulated) were identified by PPI analysis and validated by RT-qPCR. Further analysis exhibited that PTGS2 was subcellularly located in the plasma membrane. Furthermore, after aminosalicylic acid (ASA) was identified as ferroptosis inhibitor for targeting PTGS2 in senescent HUVECs, 5-ASA and 4-ASA were verified to alleviate TNF-α-induced HUVEC senescence through ferroptosis. PTGS2 might play a role in TNF-α-induced HUVEC senescence and ASA may be the potential drug for alleviating TNF-α-induced HUVEC senescence through ferroptosis.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"888-902"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141300548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Education Workshop. 教育研讨会。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2024-09-01 DOI: 10.1007/s11626-024-00953-9

引用次数: 0

Cedrol alleviates postmenopausal osteoporosis in rats through inhibiting the activation of the NF-κB signaling pathway. 西地孕酮通过抑制 NF-κB 信号通路的激活，缓解大鼠绝经后骨质疏松症。

IF 1.5 4区生物学

In Vitro Cellular & Developmental Biology. Animal Pub Date : 2024-09-01 Epub Date: 2024-05-30 DOI: 10.1007/s11626-024-00921-3

Zhen Zheng, Ying Fan, Jingyun Zhang, Jian Wang, Zhenyu Li

{"title":"Cedrol alleviates postmenopausal osteoporosis in rats through inhibiting the activation of the NF-κB signaling pathway.","authors":"Zhen Zheng, Ying Fan, Jingyun Zhang, Jian Wang, Zhenyu Li","doi":"10.1007/s11626-024-00921-3","DOIUrl":"10.1007/s11626-024-00921-3","url":null,"abstract":"Pharmacological studies have shown that Cedrol (CE) exhibits extensive biological activities, including anti-inflammatory and analgesic. Moreover, it can inhibit the NF-κB pathway and the expression of various associated proteins. This study aimed to investigate the role of CE in postmenopausal osteoporosis. The results showed that intragastric administration of CE (10 and 20 mg/kg) significantly improved the bone microstructure damage and increased bone mineral density, trabecular bone volume, and bone trabecular thickness in ovariectomized (OVX) rats (p < 0.05). CE treatment additionally made a well-organized arrangement of bone trabeculae and improved its thickness and density. Compared with the OVX group, the levels of tartrate-resistant acid phosphatase from 5b and C-terminal telopeptide of type I collagen were significantly reduced by 42.75% and 49.27% in the OVX + CE rats (p < 0.05). TRAP staining visually showed that the number of osteoclasts in the femur tissue of CE-treated rats was less than that of the OVX group. The expressions of nuclear factor of activated T-cells, cytoplasmic 1, acid phosphatase 5, and cathepsin K in OVX + CE rats were significantly decreased by 51.61%, 46.07%, and 50.34% compared to the OVX group (p < 0.01). In addition, CE intervention effectively reduced the phosphorylation levels of P65 and IκBα and inhibited the NF-κB signaling pathway. Meanwhile, CE diminished the number of multinucleated osteoclasts induced by receptor activator for nuclear factor-κB ligand and hindered cell fusion as well as nuclear translocation of osteoclast precursor cells P65. In conclusion, CE inhibits osteoclastogenesis by suppressing the activation of the NF-κB signaling pathway, thereby alleviating postmenopausal osteoporosis.","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"903-915"},"PeriodicalIF":1.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0