脂肪来源的干细胞调节线粒体动力学,以减轻HFF-1细胞的衰老。

IF 1.5 4区 生物学 Q4 CELL BIOLOGY
Qi Luo, Ling Liu
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引用次数: 0

摘要

本研究的目的是探讨脂肪源性干细胞(ASCs)如何调节线粒体结构和功能,以及这种调节对减缓细胞衰老的影响。H2O2诱导HFF-1细胞建立细胞衰老模型,并添加ASCs或Mdivi-1(线粒体裂变抑制剂)。MTT检测细胞增殖;流式细胞术检测线粒体膜电位、细胞凋亡和细胞周期;试剂盒测定ATP产量;ELISA检测各组血清白细胞介素-6 (IL-6)、白细胞介素1β (IL-1β)、肿瘤坏死因子α样(TNF-α)、谷胱甘肽(GSH)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平;Western blotting和qRT-PCR检测蛋白和mRNA的表达水平;β-半乳糖苷酶染色观察细胞衰老程度。与正常HFF-1细胞相比,衰老的HFF-1细胞增殖能力较弱,凋亡明显,g1 - g1细胞周期阻滞。这些细胞还表现出较低的线粒体膜电位和ATP的产生,较高的炎症因子表达,氧化损伤和衰老水平的增加。Mdivi-1或ASCs治疗可增强HFF-1细胞的增殖,减少凋亡和细胞周期阻滞,增加线粒体膜电位和ATP的产生,降低炎症因子的表达,减轻氧化应激,从而降低细胞衰老程度。同时干预Mdivi-1和ASCs进一步减少细胞衰老的影响。综上所述,ASCs调节线粒体动力学(促进线粒体融合和抑制线粒体裂变),增加ATP的产生,上调线粒体膜电位,从而减轻HFF-1细胞衰老引起的细胞周期阻滞、细胞凋亡、炎症反应和氧化应激。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Adipose-derived stem cells regulate mitochondrial dynamics to alleviate the aging of HFF-1 cells.

The objective of this study is to explore how adipose-derived stem cells (ASCs) regulate mitochondrial structure and function and the impact of this regulation on slowing cellular senescence. HFF-1 cells were induced by H2O2 to establish a cellular senescence model, and ASCs or Mdivi-1 (mitochondrial fission inhibitor) was added. MTT examined the cell proliferation; flow cytometry detected mitochondrial membrane potential as well as apoptosis and cell cycle; kit measured ATP production; ELISA analyzed the levels of interleukin-6 (IL-6), interleukin 1 beta (IL-1β), tumor necrosis factor alpha-like (TNF-α), glutathione (GSH), malondialdehyde (MDA), and superoxide dismutase (SOD); Western blotting and qRT-PCR detected the expression of protein and mRNA levels; and β-galactosidase staining observed the degree of cellular senescence. Compared to normal HFF-1 cells, senescent HFF-1 cells exhibited weaker proliferative capacity, marked apoptosis, and G0-G1 cell cycle arrest. These cells also showed lower mitochondrial membrane potential and ATP production, higher expression of inflammatory factors, oxidative damage, and increased levels of senescence. Treatment with Mdivi-1 or ASCs enhanced HFF-1 cell proliferation, reduced apoptosis and cell cycle arrest, increased mitochondrial membrane potential and ATP production, decreased the expression of inflammatory factors, and mitigated oxidative stress, thereby reducing the degree of cellular senescence. Concurrent intervention with Mdivi-1 and ASCs further diminishes the impacts of cellular senescence. In conclusion, ASCs regulate mitochondrial dynamics (promoting mitochondrial fusion and inhibiting mitochondrial fission), enhance ATP production, and upregulate mitochondrial membrane potential, thereby alleviating cell cycle arrest, apoptosis, inflammatory responses, and oxidative stress induced by senescence in HFF-1 cells.

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来源期刊
CiteScore
3.70
自引率
4.80%
发文量
96
审稿时长
3 months
期刊介绍: In Vitro Cellular & Developmental Biology - Animal is a journal of the Society for In Vitro Biology (SIVB). Original manuscripts reporting results of research in cellular, molecular, and developmental biology that employ or are relevant to organs, tissue, tumors, and cells in vitro will be considered for publication. Topics covered include: Biotechnology; Cell and Tissue Models; Cell Growth/Differentiation/Apoptosis; Cellular Pathology/Virology; Cytokines/Growth Factors/Adhesion Factors; Establishment of Cell Lines; Signal Transduction; Stem Cells; Toxicology/Chemical Carcinogenesis; Product Applications.
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