In Vitro Cellular & Developmental Biology. Animal最新文献

{"title":"Establishment and characterization of novel spontaneously immortalized larval cell lines from sablefish Anoplopoma fimbria.","authors":"Eric R Friesen, Amy K Long, Kyle A Garver","doi":"10.1007/s11626-024-00975-3","DOIUrl":"10.1007/s11626-024-00975-3","url":null,"abstract":"<p><p>Sablefish Anoplopoma fimbria is a groundfish of the North Pacific Ocean typically found in sea floor habitat at depths to 2700 m. Prized as a food fish with exceptionally high market value, sablefish aquaculture has been sought to provide a sustainable source of this fish to meet market demands. While commercial culture has successfully produced market-sized fish in Pacific coastal environments, production has been hampered by disease and the overall lack of information on sablefish health and immunology. To begin to address these knowledge gaps, herein we describe the isolation and characterization of spontaneously immortalized sablefish larval cell lines (AFL). Six sublines were established from pools of early yolk-sac larvae, while attempts to develop tissue-specific-derived cell lines were unsuccessful. The six yolk-sac larval cell lines each display two morphologies in culture, an elongated fibroblast-like cell type, and a rounded squamous or epithelial-like cell type. Cytogenetic characterization suggests that both cell types are diploid (2n = 48) with 24 pairs of chromosomes, 23 pairs of autosomes, and 1 pair of sex chromosomes. A small proportion (11%) of AFL cells display tetraploidy. Incubation temperature and medium composition experiments revealed HEPES buffered L-15 media containing 10-20% FBS at temperatures between 15 and 18° C yielded optimal cell growth. These growth characteristics suggest that sablefish larval cells display a robustness for varying growth conditions. The establishment of AFL cell lines provides a foundational tool to study the physiology, health, immunology, and cell and molecular biology of sablefish.</p>","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"81-92"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11799106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginsenoside Re promotes proliferation of murine bone marrow mesenchymal stem cells in vitro through estrogen-like action: comment.","authors":"Hinpetch Daungsupawong, Viroj Wiwanitkit","doi":"10.1007/s11626-024-00982-4","DOIUrl":"10.1007/s11626-024-00982-4","url":null,"abstract":"","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"117-118"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of modified human hemangioma tissue cultures and human umbilical vein endothelial cell cultures to gain mechanistic insights into imiquimod treatment for infantile hemangioma.","authors":"Abby Meyer, Lindsey Mortensen, Kimberly A Miller, Wendy A Miller, Ryan F Fader, Beverly R Wuertz, Frank G Ondrey","doi":"10.1007/s11626-024-00978-0","DOIUrl":"10.1007/s11626-024-00978-0","url":null,"abstract":"<p><p>Infantile hemangiomas (IH) are a common entity encountered by dermatologists, otolaryngologists, and other surgeons. Oral propranolol is a mainstay of treatment for IH and is well-tolerated, though propranolol-refractory IH and other drug-related adverse events are documented and can limit its usage. There are few in vitro testing systems for putative treatment agents. To address this, we modified a tissue culture system for human hemangioma treatment testing to evaluate the treatment impact of the immune modifier, imiquimod. Human umbilical vein endothelial cells (HUVEC) and hemangioma cultures were treated with several concentrations of imiquimod followed by MTT assays, reporter gene assays, PCR, ELISA, and Western blotting for IL-8, VEGF, Cyclin D1, and IFNα and immunohistochemistry for Cyclin D1 and Ki-67. HUVEC showed acute decreases in IL-8, VEGF, and Cyclin D1 promoter activity and increases in IFNα mRNA after imiquimod treatment. Hemangioma samples showed no change in Ki-67 or Cyclin D1 staining after treatment with imiquimod after 27 d, with significantly increased IL-8 and VEGF. From this preliminary analysis, we discerned that hemangioma tissues can be grown in tissue culture and used for drug treatment studies. We also conclude acute and chronic modulation of cell cycle, angiogenesis factors, and immunostimulatory conditions may be associated with imiquimod mechanisms of action in hemangioma involution.</p>","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"36-45"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomal miR-590-3p derived from bone marrow mesenchymal stem cells promotes osteoblast differentiation and osteogenesis by targeting TGFBR1.","authors":"Dandan Luo, Wantao Xie, Xiaoli He, Xiangui Zhou, Peng Ye, Peng Wang","doi":"10.1007/s11626-024-00985-1","DOIUrl":"10.1007/s11626-024-00985-1","url":null,"abstract":"<p><p>Bone marrow mesenchymal stem cells (BMSCs) have been verified to be essential factors regulating osteogenic functions, which is mainly attributed to their secretion of extracellular vesicles. Exosomes derived from BMSCs (BMSCs-Exo) contribute to osteoblast functions that are critical for improving bone defect. Our current study aims to investigate the molecular mechanism dominated by BMSCs-Exo that affects osteoblast differentiation and osteogenesis. The first step this study validated that BMSCs co-culture enhanced the differentiation ability of osteoblast and promoted bone mineralization, while these tendencies were abolished after GW4869 treatment. Next, the BMSCs-Exo was isolated and identified by TEM observation, insight detection, and western blot analysis. Furthermore, BMSCs-Exo treatment could efficiently promote the differentiation ability and the bone mineralization of osteoblasts, decrease the mRNA levels of Collagen I and Collagen III, and increase the levels of osteogenic proteins, including alkaline phosphatase (ALP), Turning Bone Morphogenetic Protein 2 (BMP2), Bone sialoprofein (BSP), osteocalcin (OCN), and osterix (OSX). However, the abovementioned effects of BMSCs-Exo could be abolished by miR-590-3p silencing. Mechanistic analysis unmasked the negative regulation of miR-590-3p on its downstream target TGFBR1. Finally, the effects of miR-590-3p/TGFBR1 axis on the differentiation and osteogenesis of osteoblasts were validated by rescue assays. In conclusion, the present study demonstrates that exosomal miR-590-3p secreted by BMSCs can induce osteoblast differentiation and osteogenesis.</p>","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"46-58"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-vascularized porous gelatin-coated β-tricalcium phosphate scaffolds for bone regeneration: an in vivo and in vitro investigation.","authors":"Hanieh Nokhbatolfoghahaei, Sahar Baniameri, Reza Tabrizi, Amir-Ali Yousefi-Koma, Mohammad Mehdi Dehghan, Samira Derakhshan, Niusha Gharehdaghi, Saeed Farzad-Mohajeri, Marjan Behroozibakhsh, Arash Khojasteh","doi":"10.1007/s11626-024-00973-5","DOIUrl":"10.1007/s11626-024-00973-5","url":null,"abstract":"<p><p>Vascularization is vital in bone tissue engineering, supporting development, remodeling, and regeneration. Lack of vascularity leads to cell death, necessitating vascularization strategies. Angiogenesis, forming new blood vessels, provides crucial nutrients and oxygen. Pre-vascularized gelatin-coated β-tricalcium phosphate (G/β-TCP) scaffolds show promise in bone regeneration and vascularization. Our study evaluates G/β-TCP scaffolds' osteogenic and angiogenic potential in vitro and a canine model with vascular anastomosis. Channel-shaped G/β-TCP scaffolds were fabricated using foam casting and sintering of a calcium phosphate/silica slurry-coated polyurethane foam, then coated with cross-linked gelatin. Buccal fat pad-derived stem cells (BFPdSCs) were seeded onto scaffolds and assessed over time for adhesion, proliferation, and osteogenic capacity using scanning electron microscopy (SEM), 4,6-diamidino-2-phenylindole (DAPI) staining, Alamar blue, and alkaline phosphatase (ALP) assays. Scaffolds were implanted in a canine model to evaluate osteogenesis and angiogenesis by histology and CT scans at 12 wk. Our studies showed preliminary results for G/β-TCP scaffolds supporting angiogenesis and bone regeneration. In vitro analyses demonstrated excellent proliferation/viability, with BFPdSCs adhering and increasing on the scaffolds. ALP activity and protein levels increased, indicating osteogenic differentiation. Examination of tissue samples revealed granulation tissue with a well-developed vascular network, indicating successful angiogenesis and osteogenesis was further confirmed by a CT scan. In vivo, histology revealed scaffold resorption. However, scaffold placement beneath muscle tissue-restricted bone regeneration. Further optimization is needed for bone regeneration applications.</p>","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"67-80"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose-derived cells surpass muscle-derived cells in primary cell isolation efficacy.","authors":"Lanlan Bai, Ken Sawai, Tetsuta Tani, Mayuko Nakai, Himari Matsusaka, Keiko Ito, Hiroshi Tomita, Eriko Sugano, Taku Ozaki, Tomokazu Fukuda","doi":"10.1007/s11626-024-00979-z","DOIUrl":"10.1007/s11626-024-00979-z","url":null,"abstract":"","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"1-7"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RO4929097 inhibits NICD3 to alleviate pulmonary hypertension via blocking Notch3/HIF-2α/FoxM1 signaling pathway.","authors":"Hao Zhu, Cheng Li, Fang Hu, Lifu Wu, Ling Wu, Meihua Zhou, Wei Liu, Aiguo Dai","doi":"10.1007/s11626-024-00976-2","DOIUrl":"10.1007/s11626-024-00976-2","url":null,"abstract":"<p><p>Pulmonary hypertension (PH) is a condition in which the smooth muscle cells (SMCs) in the pulmonary arteries multiply excessively, causing the arteries to narrow. This can ultimately result in right heart failure and premature death. Notch3 is an important factor involved in pulmonary vascular remodeling in PH. RO4929097, as a γ-secretase inhibitor that inhibits Notch3 signaling pathway, may be a potential drug for the treatment of PH, but its feasibility and related mechanism of action need to be further investigated. In vitro modeling by hypoxic incubation of human pulmonary artery SMCs (HPASMCs). RO4929097 and plasmids including overexpression-NICD3 (oe-NICD3) and NICD3 small interfering RNA (siRNA) were used to alter the expression of NICD3, and HIF-2α inhibitor PT-2385 was used to alter the expression of HIF-2α. Western blot, EdU incorporation assay was used to investigate the alteration of NICD3, HIF-2α, FoxM1 protein expression, and cell proliferation. The severity of PH in rats was assessed by measuring the weight ratio of right ventricle (RV) to left ventricle (LV) and septum (S) (RV/[LV + S]) and hematoxylin-eosin (H&E) staining of lung tissues in a hypoxia-induced PH rat model. We first determined that hypoxia induction for 48 h had the strongest induction of NICD3 and Notch3 in HPASMCs, and the strongest inhibition by 10 μM RO4929097. Treatment of HPASMCs under hypoxic conditions with RO4929097 inhibited hypoxia-induced expression of NICD3, HIF-2α, FoxM1, and proliferation of HPASMCs. The inhibitory effect of RO4929097 was reversed after overexpression of NICD3 in HPASMCs. Further, we found that PT-2385 reversed the promotional effect of overexpression of NICD3 on the proliferation of HPASMCs. In vivo experiments, hypoxia-induced PH rats treated with RO4929097 showed a reduction in right ventricular hypertrophy index (RVHI) and a return to normal pulmonary artery morphology, indicating a reduction in the severity of PH. Our data suggest that RO4929097 regulates the Notch3/HIF-2α/FoxM1 signaling pathway by inhibiting the expression of NICD3, thereby inhibiting hypoxia-induced proliferation of HPASMCs. In vivo experiments also confirmed that RO4929097 could alleviate PH as a potential therapeutic strategy.</p>","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":"107-116"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy determination of a disinfectant against channel catfish virus by in vitro and in vivo methods.","authors":"Suja Aarattuthodi, Brian Bosworth, Ganesh Kumar, Anita Nalamalapu","doi":"10.1007/s11626-024-01003-0","DOIUrl":"https://doi.org/10.1007/s11626-024-01003-0","url":null,"abstract":"<p><p>Channel catfish virus (CCV) poses a significant threat to catfish culture. Lack of effective vaccines and antiviral treatments necessitates effective disinfection strategies to mitigate its spread. In vitro trials indicated the virus to be inactivated at high temperatures, but was infectious at 40°C. This study evaluated the efficacy of a commercial disinfectant against CCV using both in vitro and in vivo approaches. In vitro experiments assessed the virucidal activity of the disinfectant against CCV in channel catfish ovary (CCO) cells, while in vivo trials evaluated its effectiveness in reducing viral transmission and mortality among channel and hybrid catfish fingerlings. Results indicated that the disinfectant was effective in inactivating the virus at the tested concentrations and improved the survival of fish exposed to the virus. This study provides critical insights into selecting appropriate disinfection protocols to enhance biosecurity in catfish hatchery settings and to mitigate CCV transmission.</p>","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SIVB's 2024 In Vitro Biology Meeting: Program, Schedule, Awards, and Acknowledgements.","authors":"","doi":"10.1007/s11626-024-01007-w","DOIUrl":"https://doi.org/10.1007/s11626-024-01007-w","url":null,"abstract":"","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of polyvinyl alcohol/gelatin/chitosan hydrogel for tissue engineering and wound healing applications using a fish cell line model.","authors":"Sivaraj Mithra, Ali Asna Jabeen, Vinay Kumar, Seepoo Abdul Majeed, Manickam Balu Balaji, Sugumar Vimal, Dawood Mubeen Sultana, Sakvai Mohammed Safiullah, Gani Taju, Azeez Sait Sahul Hameed","doi":"10.1007/s11626-024-00996-y","DOIUrl":"https://doi.org/10.1007/s11626-024-00996-y","url":null,"abstract":"<p><p>Chitosan-based hydrogels have gained considerable attention in biomedical research due to their inherent biocompatibility, biodegradability, and non-toxicity. When combined with polyvinyl alcohol (PVA), the resulting hydrogels exhibit superior mechanical strength, elasticity, and swelling capacity, making them highly suitable for a range of applications, including wound healing, tissue engineering, and controlled drug delivery. In this study, we synthesized and characterized a novel PVA/gelatin/chitosan (PVA/G/C) hydrogel composite using a series of analytical techniques such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray analysis (EDAX). The morphological, structural, and compositional analyses confirmed the successful formation of a homogenous, porous network conducive to cell proliferation and nutrient diffusion. In this study, polyvinyl alcohol/gelatin/chitosan-based hydrogels were prepared to study the potential for micro-tissue formation and wound healing application using Danio rerio gill (DrG) and Danio rerio fin (DrF) cell lines, respectively. Overall, the findings indicated the potential use of PVA/G/C hydrogel films as wound dressings. The idea of creating physically cross-linked hydrogels of PVA and chitosan without using any harmful organic chemicals or solvents is the novelty of this work. This study highlights the versatility and potential of PVA/G/C hydrogels, not only as a promising material for wound healing and drug delivery but also as an effective scaffold for tissue engineering applications.</p>","PeriodicalId":13340,"journal":{"name":"In Vitro Cellular & Developmental Biology. Animal","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}