Indian Journal of Medical Microbiology最新文献_第8页

Comparative performance evaluation of random access and real-time PCR techniques in the diagnosis of BK virus infections in transplant patients 随机存取和实时 PCR 技术在移植患者 BK 病毒感染诊断中的性能比较评估

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2024-07-31 DOI: 10.1016/j.ijmmb.2024.100687

Erdal Sanlidag , Ayse Arikan , Murat Sayan

{"title":"Comparative performance evaluation of random access and real-time PCR techniques in the diagnosis of BK virus infections in transplant patients","authors":"Erdal Sanlidag , Ayse Arikan , Murat Sayan","doi":"10.1016/j.ijmmb.2024.100687","DOIUrl":"10.1016/j.ijmmb.2024.100687","url":null,"abstract":"<div><h3>Purpose</h3><p>The study aims to compare random-access NeuMoDx values with artus qPCR values to validate the accuracy of NeuMoDx as an alternative to qPCR and provide an equation to convert copies/ml to IU/ml measurements.</p></div><div><h3>Methods</h3><p>A total of 95 samples, including 61 transplant patient samples (n = 23 urine, n = 38 plasma) as the study group, 28 BKPyV-free samples as the control group, and six quality control samples, were included. One-Way ANOVA, Pearson correlation, Bland-Altman, Passing-Bablok, Deming regression analyses were used for statistical evaluation.</p></div><div><h3>Results</h3><p>Of 95 samples, 46 (48 %) were positive with NeuMoDx, while 40 (42 %) were positive with artus qPCR. Both techniques were statistically similar (p > 0.05). Deming correlation analysis (r = 0.9590), Passing Bablok and Bland Altman analyses demonstrated a strong correlation between NeuMoDx and artus values. The equation that provides the conversion between NeuMoDx and artus qPCR values was NeuMoDx= (1.12965 x artus qPCR) – 0.55016. BKPyV infections remain a concern for transplant patients globally, and effective new diagnostic methods are required.</p></div><div><h3>Conclusions</h3><p>Consistency between the results of NeuMoDx and qPCR confirms that NeuMoDx may be a valuable alternative for detecting BKPyV to prevent viral propagation. Our findings may allow converting copy/ml results to IU/ml for diagnosing and monitoring BKPyV infections in transplant patients.</p></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"51 ","pages":"Article 100687"},"PeriodicalIF":1.4,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Letter to Editor 致编辑的信

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2024-07-31 DOI: 10.1016/j.ijmmb.2024.100667

Jayashree Jamatia, Bharati Dalal, Mahadevan Kumar, Deepa Devhare

引用次数: 0

Pulmonary cryptococcosis in chronic kidney disease 慢性肾脏病中的肺隐球菌病。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2024-07-29 DOI: 10.1016/j.ijmmb.2024.100688

Masoom Nathani , Vikas Manchanda , Akash Jorwal , Sonal Saxena , Suresh Kumar

引用次数: 0

Rare Culture positivity of Mycoplasma pneumoniae from a Community-acquired atypical pneumonia case 一例社区获得性非典型肺炎病例中罕见的肺炎支原体培养阳性。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2024-07-23 DOI: 10.1016/j.ijmmb.2024.100669

Sajitha Venkatesan, Padma Das

引用次数: 0

Implementation of surgical site infection care bundle in patients undergoing gastrointestinal surgeries 在接受胃肠道手术的患者中实施手术部位感染护理包。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2024-07-16 DOI: 10.1016/j.ijmmb.2024.100670

Sarumathi Dhandapani , Kalayarasan Raja , Ketan Priyadarshi , Apurba Sankar Sastry

{"title":"Implementation of surgical site infection care bundle in patients undergoing gastrointestinal surgeries","authors":"Sarumathi Dhandapani , Kalayarasan Raja , Ketan Priyadarshi , Apurba Sankar Sastry","doi":"10.1016/j.ijmmb.2024.100670","DOIUrl":"10.1016/j.ijmmb.2024.100670","url":null,"abstract":"<div><h3>Purpose</h3><p>Surgical site infection (SSI) is one of the frequent healthcare associated infections linked with significant morbidity, prolonged hospitalization, and death. SSI can be reduced by implementation of customized care bundle components as per standard guidelines. Hence this study was undertaken with the objective to implement care bundle in patients undergoing elective gastrointestinal surgeries and assess their impact on SSI rate.</p></div><div><h3>Methods</h3><p>The study was an interventional study conducted in the department of surgical gastroenterology for 8 months. Sample size was calculated to be 196 and only elective surgeries are included. CDC NHSN 2023 guidelines are used for surveillance of SSI and global guidelines for prevention of SSI was used for preparation of list of pre-operative, intraoperative and post-operative care bundle components and were implemented before the start of the study.</p></div><div><h3>Results</h3><p>Overall SSI rate and compliance to SSI care bundle in this study are 13.8% and 28.6%, respectively. When compared with the baseline SSI rate of 19.4%, there is reduction of 28.9% in SSI rate after the implementation of care bundle. <em>Escherichia coli</em> (54.2%) is the most commonly isolated organism. Care bundle non-compliant surgeries are associated with 2.3 times (relative risk-2.3) increased risk of SSI<strong>.</strong> There is fluctuating trend in compliance of care bundle and SSI rates across months.</p></div><div><h3>Conclusion</h3><p>This study shows the importance of implementation of set of care bundle for prevention of SSI which can be customized and adapted for reducing SSI.</p></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"51 ","pages":"Article 100670"},"PeriodicalIF":1.4,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and genomic characterization of a small colony variant of Klebsiella pneumoniae isolated from urine of a prostate cancer case 从一例前列腺癌患者尿液中分离出的肺炎克雷伯氏菌小菌落变异体的表型和基因组特征。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2024-07-16 DOI: 10.1016/j.ijmmb.2024.100664

引用次数: 0

Corrigendum to "Current trends in antimicrobial resistance of ESKAPEEc pathogens from bloodstream infections - Experience of a tertiary care centre in North India" [Indian J. Med. Microbiol. 50 (July-August 2024), 100647]. 血流感染 ESKAPEEc 病原体抗菌药耐药性的当前趋势--北印度一家三级护理中心的经验"[《印度医学微生物学杂志》（Indian J. Med. Microbiol. 50 (July-August 2024)，100647）]更正。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2024-07-15 DOI: 10.1016/j.ijmmb.2024.100662

Veenu Gupta, Menal Gupta, Rama Gupta, Jyoti Chaudhary

引用次数: 0

Comparative performance of biofire pneumonia panel and standard culture-based methods for diagnosing pneumonia in critically ill patients: Impact on antibiotic stewardship 用于诊断重症患者肺炎的 Biofire Pneumonia Panel 与标准培养方法的性能比较：对诊断和抗生素管理的影响。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2024-07-15 DOI: 10.1016/j.ijmmb.2024.100668

引用次数: 0

Targeted Next Generation Sequencing (tNGS) for detection of drug-resistant tuberculous meningitis: Is this sequencing technology ready for prime time? 用于检测耐药结核性脑膜炎的靶向新一代测序技术（tNGS）：这项测序技术是否已准备就绪？

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2024-07-11 DOI: 10.1016/j.ijmmb.2024.100665

Priti Kambli , Kanchan Ajbani , Amala A. Andrews , Shaoli Basu , Anjali Shetty , Tanvi Patil , Ishita Mehta , Harpreet Singh , Camilla Rodrigues

{"title":"Targeted Next Generation Sequencing (tNGS) for detection of drug-resistant tuberculous meningitis: Is this sequencing technology ready for prime time?","authors":"Priti Kambli , Kanchan Ajbani , Amala A. Andrews , Shaoli Basu , Anjali Shetty , Tanvi Patil , Ishita Mehta , Harpreet Singh , Camilla Rodrigues","doi":"10.1016/j.ijmmb.2024.100665","DOIUrl":"10.1016/j.ijmmb.2024.100665","url":null,"abstract":"<div><h3>Purpose</h3><p>Tuberculous meningitis (TBM) is the most severe form of tuberculosis (TB). Difficulty in diagnosing the condition along with other factors, increases its potential for high morbidity and mortality. Targeted Next Generation Sequencing (tNGS) generates high quality sequence read depths, enabling the identification of low-frequency alleles linked to Drug resistance (DR). The paucibacillary nature of tuberculous meningitis is a challenge for making a definitive diagnosis.</p></div><div><h3>Methods</h3><p>tNGS was performed on 20 cerebrospinal fluid (CSF) samples where, MGIT has shown Positive MTB Cultures. We simultaneously performed pyrosequencing (PSQ) and phenotypic Drug susceptibility testing (pDST) for these 20 samples.</p></div><div><h3>Results</h3><p>Sequencing results (from tNGS and PSQ) were compared with reference standards i.e. pDST. tNGS detected MTB in 7/20 (35%) CSF samples whereas, PSQ detected MTB in 17/20 (85%).</p></div><div><h3>Conclusion</h3><p>Although tNGS has ability to detect minority variants along with detection of additional targets than PSQ, PSQ remains the diagnostic choice in our tertiary lab.</p></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"51 ","pages":"Article 100665"},"PeriodicalIF":1.4,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Bacillus cereus containing nheA, hblC and cytk enterotoxin genes is associated with acute childhood gastroenteritis in Nigeria 含有 nheA、hblC 和 cytk 肠毒素基因的蜡样芽孢杆菌与尼日利亚儿童急性肠胃炎有关。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2024-07-10 DOI: 10.1016/j.ijmmb.2024.100666

Ebuka E. David , Ikechuku O. Igwenyi , Ifeanyichukwu R. Iroha , Layla F. Martins , Guillermo Uceda-Campos , Aline M. da Silva

引用次数: 0