Indian Journal of Medical Microbiology最新文献

{"title":"\"The nexus of extensively drug-resistant gram-negative bacteria and intensive care: Elucidating propagation patterns to fortify infection control measures\"","authors":"Debasish Biswal, Sandeep Mangla, Sadia Khan, Aayush Chawla, Pankhuri Kumari, Anubhav Pandey, Sharmila Sengupta","doi":"10.1016/j.ijmmb.2024.100758","DOIUrl":"10.1016/j.ijmmb.2024.100758","url":null,"abstract":"","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"53 ","pages":"Article 100758"},"PeriodicalIF":1.4,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MALDI - TOF MS for the identification of obligate anaerobes and metronidazole susceptibility of anaerobic Gram negative bacilli.","authors":"Simson D, Dhanalakshmi Gounassegarane, Rakhi Biswas","doi":"10.1016/j.ijmmb.2024.100767","DOIUrl":"https://doi.org/10.1016/j.ijmmb.2024.100767","url":null,"abstract":"<p><p>MALDI-TOF MS, though has facilitated rapid and accurate anaerobe identification, the problem of rising metronidazole resistance amongst the members of Bacteroides fragilis group is cause for concern. In this one-year study period,152 anaerobic Gram negative bacilli and 60 Gram positive anaerobes were isolated from 167 samples obtained from clinically suspected anaerobic infections. Bacteroides fragilis accounted for 56% of the total anaerobic GNB and Peptoniphilus asacchrarolyticus was the most commonly isolated Gram positive cocci. All isolates were identified by the MALDI-TOF MS except one isolate each of Clostridium and Peptostreptococcus. E-test for members of Bacteroides fragilis group, demonstrated 26.8% metronidazole resistance.</p>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":" ","pages":"100767"},"PeriodicalIF":1.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of intra-assay and inter-assay reproducibility and positive detection times of two different (BacT/Alert 3D and Autobio BC) commercial blood culture systems","authors":"Nilgün Kansak ,&nbsp;Nilay Zeynep Kalender ,&nbsp;Neslihan Arıcı ,&nbsp;Rıza Adaleti ,&nbsp;Sebahat Aksaray ,&nbsp;Handan Ankaralı ,&nbsp;Nevriye Gönüllü","doi":"10.1016/j.ijmmb.2024.100754","DOIUrl":"10.1016/j.ijmmb.2024.100754","url":null,"abstract":"<div><h3>Purpose</h3><div>In our study, we aimed to compare the performance of the BacT/Alert 3D (bioMerieux, France) system, which is currently used in our laboratory, and the Autobio BC (Autobio, China) system, which was newly introduced in our country, using standard and clinical isolates.</div></div><div><h3>Methods</h3><div>Bacterial suspension was prepared by two technicians on the same day and three consecutive days from five different standard strains with 0.5 McFarland turbidity, then serial dilution to a final concentration was adjusted and was simultaneously inoculated in aerobic blood culture bottles. The bacterial concentration was measured by making a quantitative counting plate. The same procedure was also performed for 55 clinical isolates belonging to eleven species. After simulated bacteremia with standard and clinical isolates, the growth results were confirmed by inoculation from positive blood culture bottles onto solid medium and identification was made in the next day with MALDI-TOF MS (bioMérieux). In each study, sterile saline and blood was inoculated into the bottles as a negative control to check contamination. Intra-assay and inter-assay reproducibility of recovery rates and detection times of standard strains; recovery rates and detection times of clinical isolates were compared for both systems.</div></div><div><h3>Results</h3><div>Recovery rates were 100 % in both systems, and when positive detection times were compared, it was found that there was no difference between the two devices in clinical isolates (p:0.262) but that Autobio BC gave significantly (p &lt; 0.001) earlier results in standard strains.</div></div><div><h3>Conclusions</h3><div>In our simulated bloodstream infection study, Autobio BC was found to be comparable with BacT/Alert 3D, both recovery rates and growth detection time performance were found to be very good, and it can be used in routine microbiology laboratories.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"53 ","pages":"Article 100754"},"PeriodicalIF":1.4,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pattern of Rapidly growing Mycobacteria (RGM) species isolated from clinical samples: A 10-year retrospective study in a tertiary care hospital of Bangladesh.","authors":"Lovely Barai, Mili Rani Saha, Tanjila Rahman, Marium Sukanya, Jannatul Ferdous, Adeeba Khanduker, Rokibul Hasan, TasfiaTasnim Nova","doi":"10.1016/j.ijmmb.2024.100756","DOIUrl":"https://doi.org/10.1016/j.ijmmb.2024.100756","url":null,"abstract":"<p><strong>Purpose: </strong>Infections caused by rapidly growing mycobacteria (RGM) are increasing worldwide. The study was conducted in a microbiological laboratory of Bangladesh to determine the pattern of detection of RGM from clinical samples.</p><p><strong>Methods: </strong>All laboratory culture records of RGM from 2012 to 2022 were collected retrospectively and analyzed.</p><p><strong>Results: </strong>A total 62 RGM infected patients with surgical site infection (74.1%), injection site and skin abscess (9.7%), septicemia (4.8%) and UTI (1.6%) were identified. The annual isolation frequency of RGM increased 4.8% to 29.1% in between year 2012 and 2022. RGM infected patients (14.5%) were mistakenly treated with first line anti tubercular drug before correct microbiological diagnosis (median, IQR; 3, 2-5 months). Out of 23 RGM isolates, 86.9% were M. abscessus and rest 13.1% were M. fortuitum. Most of them (≥95%) were sensitive to amikacin, linezolid, clarithromycin where as 27.1% to imipenem and ciprofloxacin, 40% to cefoxitin, 35.3% and 1.7% to doxycycline and co-trimoxazole respectively.</p><p><strong>Conclusion: </strong>Misdiagnosis or delay in diagnosis and erroneous treatment with first line anti tubercular drug may cause prolong morbidity and therapeutic failure to patients with RGM infection. So, early and appropriate diagnosis is crucial for successful outcome.</p>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":" ","pages":"100756"},"PeriodicalIF":1.4,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testing new waters in management of Stenotrophomonas maltophilia blood stream infection in a post cardiac surgery patient","authors":"Ekadashi Rajni ,&nbsp;Ashish Sharma ,&nbsp;Kriti Goyal ,&nbsp;Divyansh Gupta","doi":"10.1016/j.ijmmb.2024.100746","DOIUrl":"10.1016/j.ijmmb.2024.100746","url":null,"abstract":"<div><div>Hospital acquired infections are the most common non-cardiac complications after cardiac surgery. Organisms commonly found in this cohort of patients include <em>Klebsiella</em> spp<em>, Escherichia coli, Pseudomonas</em> spp<em>, Staphylococcus aureus, Coagulase negative Staphylococcus</em> and <em>Enterococcus</em> spp etc. We hereby present a case of post cardiac surgery patient presenting with <em>Stenotrophomonas maltophilia</em> bloodstream infection. Combination of ceftazidime-avibactam (CZA) and aztreonam(AT) was used as inactivity was exhibited to primary panel of drugs. Broth disc elution testing was done to exhibit synergy between CZA and AT. This case highlights the growing need for active interaction between the treating physician and the microbiologist.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"52 ","pages":"Article 100746"},"PeriodicalIF":1.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A vector borne, airborne and food borne infection with secondary hemophagocytic lymphohistocytosis: Case of triple infections in an immuno-competent patient","authors":"Abdul Nafey Kazi ,&nbsp;Muzeer Ahmed ,&nbsp;Muhammad Arsalan Wasim ,&nbsp;Lubna Iqbal Abbasi ,&nbsp;Fivzia Farooq Herekar ,&nbsp;Muhammad Junaid Patel","doi":"10.1016/j.ijmmb.2024.100752","DOIUrl":"10.1016/j.ijmmb.2024.100752","url":null,"abstract":"<div><div>Pakistan is endemic to several bacterial, parasitic and viral infections including tuberculosis, malaria and dengue that account for a high rate of mortality each year. These different types of infections can occur through various routes of transmission and concurrently in individuals, especially in immunocompromised but rarely among immunocompetent. We report a case of a <strong>25 year old</strong> immunocompetent female who was admitted with an initial diagnosis of dengue fever and diagnosed as having co-infection of COVID-19 and Brucella leading to secondary hemophagocytic lymphohistiocytosis (HLH). She was managed for all three infections and complications simultaneously.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"52 ","pages":"Article 100752"},"PeriodicalIF":1.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fasciolopsis buski isolated from human host, India has distinct clade based on nuclear ribosomal DNA sequences","authors":"Sandhya Chaurasiya ,&nbsp;Aradhana Singh ,&nbsp;Anurag Tiwari ,&nbsp;Tuhina Banerjee","doi":"10.1016/j.ijmmb.2024.100757","DOIUrl":"10.1016/j.ijmmb.2024.100757","url":null,"abstract":"<div><div>Fasciolopsiasis caused by <em>Fasciolopsis buski</em>, is a significant cause of morbidity. In the present study <em>F. buski</em> detected during endoscopy of a 50-year-old male patient form Uttar Pradesh, India was used for phylogenetic analysis based on 28S rDNA and ITS2 regions. The parasite was genetically similar to an isolate of pig from Meghalaya. The intra-species genetic variation for 28S rDNA was 1–3% (India), 5% (Vietnam) and for ITS2, 1–2% (India) while 17 % from Vietnam. Thus, <em>F. buski</em> from humans or pigs from India were closely related than those from Vietnam and China which were grouped into separate clades.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"52 ","pages":"Article 100757"},"PeriodicalIF":1.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Musculoskeletal melioidosis-a retrospective review of 22 cases from a tertiary care centre in South Tamilnadu","authors":"G. Vithiya ,&nbsp;G. Rajalakshmi Preethi ,&nbsp;P. Shunmuga Sundaram ,&nbsp;T. Rajendran","doi":"10.1016/j.ijmmb.2024.100750","DOIUrl":"10.1016/j.ijmmb.2024.100750","url":null,"abstract":"<div><div>A retrospective review of culture confirmed musculoskeletal melioidosis reported between December 2014 and February 2024 was conducted. 22 of 100 patients with culture proven melioidosis infection had musculoskeletal involvement. Median age was 47 years with 95.4 % being males. Osteomyelitis, septic arthritis and septic arthritis along with adjacent osteomyelitis were observed in 12, 9 and 4 cases respectively. All isolates were susceptible to meropenem and susceptible increased exposure to ceftazidime, doxycycline and cotrimoxazole. Twelve patients underwent surgical procedures. Four patients who died due to septicemia had multisystem involvement with positive blood cultures.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"52 ","pages":"Article 100750"},"PeriodicalIF":1.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuromelioidosis – A retrospective review of thirteen cases from a tertiary care centre from South India","authors":"G. Vithiya ,&nbsp;Preethi G. Rajalakshmi ,&nbsp;P Shunmuga Sundaram ,&nbsp;T. Rajendran","doi":"10.1016/j.ijmmb.2024.100751","DOIUrl":"10.1016/j.ijmmb.2024.100751","url":null,"abstract":"<div><div>Neurologic melioidosis warrants attention in view of its rarity and lethal consequences. We performed a retrospective review of culture confirmed neurologic melioidosis reported between 2017 and 2023. Thirteen patients were identified with median age 43 years and all but three were males. Brain parenchyma was involved in 57 % cases (7/13) as cerebritis, encephalitis and macro abscess or micro abscess. Three patients had myelitis with spinal cord micro abscess and cerebral venous thrombosis in three other patients. Five patients died during hospitalization. Two were discharged against medical advice due to financial constraints. Six survivors were followed between one and eight months.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"52 ","pages":"Article 100751"},"PeriodicalIF":1.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic approach of ertapenem and flucloxacillin or cefazolin for addressing persistent methicillin susceptible Staphylococcus aureus bacteremia","authors":"Yamuna Devi Bakthavatchalam ,&nbsp;Rajiv Karthik ,&nbsp;Anand Ashok ,&nbsp;Kamini Walia (Scientist G) ,&nbsp;Harathi Ragothaman ,&nbsp;Soniya Krishnamoorthy ,&nbsp;Yuvasri Manokaran ,&nbsp;Balaji Veeraraghavan","doi":"10.1016/j.ijmmb.2024.100755","DOIUrl":"10.1016/j.ijmmb.2024.100755","url":null,"abstract":"<div><div>In the present study, we determine the synergistic activity of ertapenem with cefazolin and flucloxacillin against type A beta-lactamase producing Methicillin susceptible <em>Staphylococcus aureus</em> isolate. In the time kill assay, at standard inoculum, ertapenem with cefazolin showed &gt;1 log kill and ertapenem with flucloxacillin demonstrated &gt;2 log kill. When tested at high inoculum, both combinations achieved &gt;1 log kill at 1x MIC.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"52 ","pages":"Article 100755"},"PeriodicalIF":1.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}