Utility of multiplex PCR for rapid diagnosis of acute bacterial meningitis: A North Indian tertiary care experience

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology Pub Date : 2025-07-10 DOI:10.1016/j.ijmmb.2025.100915

Debasish Biswal , Hitender Gautam , Varun Kumar , Sonu Tyagi , Yogesh Kumar , Sarita Mohapatra , Seema Sood , Benu Dhawan , Bimal Kumar Das

引用次数: 0

Abstract

Rapid diagnosis of acute bacterial meningitis (ABM) remains challenging. This study evaluated multiplex PCR versus conventional methods. A total of 1248 cerebrospinal fluid samples from suspected ABM patients were tested using Gram stain, culture, and multiplex PCR targeting Streptococcus pneumoniae, Haemophilus influenzae type b, Neisseria meningitidis, Streptococcus agalactiae, and 16S rRNA gene. Multiplex PCR detected pathogens in 9.3 % of samples, compared to 2.3 % by culture and 1.5 % by Gram stain. PCR identified H. influenzae (13), S. pneumoniae (7), N. meningitidis (1), S. agalactiae (1). With 90 % sensitivity and 92.6 % specificity, multiplex PCR is a valuable complementary diagnostic tool for ABM.

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多重聚合酶链反应快速诊断急性细菌性脑膜炎的效用：北印度三级保健经验

快速诊断急性细菌性脑膜炎（ABM）仍然具有挑战性。本研究对多重PCR与传统方法进行了比较。采用革兰氏染色法、培养法和针对肺炎链球菌、b型流感嗜血杆菌、脑膜炎奈瑟菌、无乳链球菌和16S rRNA基因的多重PCR对疑似ABM患者的1248份脑脊液样本进行检测。多重PCR在9.3%的样品中检测到病原体，而培养法为2.3%，革兰氏染色法为1.5%。PCR鉴定出流感嗜血杆菌（13株）、肺炎链球菌（7株）、脑膜炎奈瑟菌（1株）、无乳链球菌（1株）。多重PCR具有90%的敏感性和92.6%的特异性，是ABM有价值的补充诊断工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Medical Microbiology IMMUNOLOGY-

CiteScore

2.20

自引率

0.00%

发文量

154

审稿时长

73 days

期刊介绍： Manuscripts of high standard in the form of original research, multicentric studies, meta analysis, are accepted. Current reports can be submitted as brief communications. Case reports must include review of current literature, clinical details, outcome and follow up. Letters to the editor must be a comment on or pertain to a manuscript already published in the IJMM or in relation to preliminary communication of a larger study. Review articles, Special Articles or Guest Editorials are accepted on invitation.