{"title":"Genetic diversity of Thailand reserved mulberry germplasm based on morphological characteristics and newly developed EST-SSR and SRAP markers","authors":"Nutdanai Putthisawong, Surapa Nutthapornnitchakul, Natnicha Thumthuan, Chatuporn Kuleung, Athipat Ngernmuen, Piyama Tasanasuwan, Chatchawan Jantasuriyarat","doi":"10.1007/s13580-024-00599-2","DOIUrl":"https://doi.org/10.1007/s13580-024-00599-2","url":null,"abstract":"<p>Mulberry (<i>Morus</i> L.) is an important crop for the sericulture industry, serving as the primary food source for the silkworm <i>Bombyx mori</i> L. Thailand has a long history of practicing sericulture and has imported and improved upon many indigenous cultivars to create new hybrid offspring. It is crucial to understand the genetic divergence of these accessions for their conservation and utilization in selection and breeding. In this study, 85 representative mulberry accessions in Thailand were observed morphology and analyzed for their genetic relationships using SRAP and EST-SSR markers. The findings indicate that the morphological traits of Thai mulberry are distinctive enough to differentiate between <i>M. macroura</i> Miq. and wild hybrid mulberry, and a group consisting of <i>M. alba</i> L. and <i>M. australis</i> Poir., and their hybrids. 12 SRAP primer combinations produced 193 polymorphic amplicons with an average of 17.0 bands per primer set, and the mean of PIC was 0.259. Eleven novel EST-SSR primers generated 35 amplicons with an average of 3.2 alleles per primer set, and the average PIC was 0.139. The dendrogram obtained using the UPGMA algorithm in R studio showed that the wild and wild hybrid mulberry were genetically distant from the domesticated species studied here. These findings have important implications for the characterization, improvement, molecular systematics, and conservation of Thai mulberry germplasm.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140055385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daniel Tran, Elena Najdenovska, Fabien Dutoit, Carrol Plummer, Nigel Wallbridge, Marco Mazza, Cédric Camps, Laura Elena Raileanu
{"title":"Advanced assessment of nutrient deficiencies in greenhouse with electrophysiological signals","authors":"Daniel Tran, Elena Najdenovska, Fabien Dutoit, Carrol Plummer, Nigel Wallbridge, Marco Mazza, Cédric Camps, Laura Elena Raileanu","doi":"10.1007/s13580-023-00589-w","DOIUrl":"https://doi.org/10.1007/s13580-023-00589-w","url":null,"abstract":"<p>Nutrient deficiencies are one of the main causes of significant reductions in commercial crop production by affecting associated growth factors. Proper plant nutrition is crucial for crop quality and yield therefore, early and objective detection of nutrient deficiency is required. Recent literature has explored the real-time monitoring of plant electrical signal, called electrophysiology, applied on tomato crop cultivated in greenhouse. This sensor allows to identify the stressed state of a plant in the presence of different biotic and abiotic stressors by employing machine learning techniques. The aim of this study was to evaluate the potential of electrophysiology signal recordings acquired from tomato plants growing in a production greenhouse environment, to detect the stress of a plant triggered by the deficiency of several main nutrients. Based on a previously proposed workflow consisting of continuous acquisition of electrical signal then application of machine learning techniques, the minimum signal features was evaluated. This study presents classification models that are able to distinguish the plant’s stressed state with good accuracy, namely 78.5% for manganese, 78.1% for iron, 89.6% for nitrogen, and 78.1% for calcium deficiency, and therefore suggests a novel path to detect nutrient deficiencies at an early stage. This could constitute a novel practical tool to help and assist farmers in nutrition management.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"2 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139762491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antioxidant and α-glucosidase inhibitory potential of the pollen of Hibiscus spp.","authors":"","doi":"10.1007/s13580-023-00578-z","DOIUrl":"https://doi.org/10.1007/s13580-023-00578-z","url":null,"abstract":"<h3>Abstract</h3> <p><em>Hibiscus</em> species are flowering plants of the Malvaceae family. <em>Hibiscus</em> are widely distributed in tropical and subtropical regions and play an important role not only in ornamental aspects but also traditional medicine. In this study, seven compounds, mukungarrole (<strong>1</strong>), a new compound and six known flavonoid derivatives (<strong>2</strong>–<strong>7</strong>) were isolated from <em>Hibiscus syriacus</em> pollen. Among the isolated compounds, kaempferol (<strong>2</strong>) and vincetoxicoside B (<strong>3</strong>) showed antioxidant activity. Additonally, the new compound, mukungarrole (<strong>1</strong>), along with kaempferol (<strong>2</strong>) and isorhamnetin-7-<em>O</em>-rhamnoside (<strong>5</strong>), showed α-glucosidase inhibitory activity and its interaction with α-glucosidase was confirmed using molecular docking analysis. Further comparison of five varieties of <em>Hibiscus</em> spp. showed that all five species contained vincetoxicoside B (<strong>3</strong>) and kaempferol-7-<em>O</em>-rhamnoside (<strong>4</strong>) as major components and exhibited antioxidant and α-glucosidase inhibitory activities. However, there was differences in the content of vincetoxicoside B (<strong>3</strong>) and kaempferol-7-<em>O</em>-rhamnoside (<strong>4</strong>) and minor components depending on of <em>Hibiscus</em> variety, which contributed to differences in efficacy. This study proves that <em>H. syriacus</em> pollen and its constituents have antioxidant and α-glucosidase inhibitory potentials and the content and efficacy vary depending on <em>Hibiscus</em> species.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"38 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139668340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hydrogen-based irrigation increases yield and improves quality of Chinese cabbage by enhancing nutrient composition and antioxidant capabilities","authors":"Ziyu Liu, Genmei Chen, Enxin Yang, Longna Li, Yan Zeng, Xu Cheng, Didier Pathier, Guoyi Xu, Wenbiao Shen","doi":"10.1007/s13580-023-00591-2","DOIUrl":"https://doi.org/10.1007/s13580-023-00591-2","url":null,"abstract":"<p>To meet the increasing demands for higher-value and nutrient-rich vegetables, this work aims to investigate whether molecular hydrogen (H<sub>2</sub>)-based irrigation, in the form of hydrogen nanobubble water (HNW), could improve yield and quality of Chinese cabbage (<i>Brassica chinensis</i> L.). Field trials showed that compared to surface water (SW) irrigation (control group), HNW irrigation significantly increased Chinese cabbage yield by 32.70 ± 12.15%, and the single plant weight, height, the maximum leaf length and width were also significantly increased. Higher nutritional value, including the increased contents of soluble sugar, soluble protein, mineral nutrients (P, Fe, Zn, and Cu), and secondary metabolites (total carotenoids, proanthocyanidins, phenols, flavonoids, and ascorbic acid), as well as decreased nitrate content and higher antioxidant capability (assessed by DPPH, ABTS and FRAP values) were also observed. Molecular evidence further revealed that the expressions of genes related to Fe, Zn, and P absorption as well as ascorbic acid biosynthesis were obviously up-regulated, consistent with changes in Fe, Zn, P, and ascorbic acid accumulation achieved after HNW irrigation. These results clearly suggested that HNW might have a practical value in improving yield and quality of horticultural crops.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"225 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139668158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yoonseo Lim, Myeong-Gyun Seo, Sehee Lee, Jeong-Tak An, Ho-Young Jeong, Yoonwoo Park, Chanhui Lee, Choon-Tak Kwon
{"title":"Comparative yield evaluation of mini-tomato cultivar in two hydroponic systems","authors":"Yoonseo Lim, Myeong-Gyun Seo, Sehee Lee, Jeong-Tak An, Ho-Young Jeong, Yoonwoo Park, Chanhui Lee, Choon-Tak Kwon","doi":"10.1007/s13580-023-00564-5","DOIUrl":"https://doi.org/10.1007/s13580-023-00564-5","url":null,"abstract":"<p>Recent agricultural technology advancements have introduced innovative techniques like vertical farming and plant factories, offering precision agriculture. However, leafy green vegetables are dominantly grown in vertical farms with high-tech hydroponics, and fruit crops with relatively large plant sizes are neglected in vertical farming. To broaden the range of crops suitable for vertical farming, it is crucial to not only explore and develop novel varieties but also establish tailored cultivation systems. We cultivated the diminutive \"Micro-Tom\" tomato variety using two different hydroponic systems and assessed its productivity. After conducting four cultivation cycles and three yield trials, we verified the viability of hydroponic cultivation for mini-tomato plants. Our results suggest that enclosed vertical farms, where the external environment is strictly controlled, are more suitable for cultivating small fruit crops. These findings offer promising insights for the future application of hydroponics and vertical farming to various crops.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"12 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139668256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification and diversity analysis of interspecific citrus rootstock hybrids with combination of morphological traits and microsatellite markers","authors":"","doi":"10.1007/s13580-023-00588-x","DOIUrl":"https://doi.org/10.1007/s13580-023-00588-x","url":null,"abstract":"<h3>Abstract</h3> <p>Rough lemon is indigenous and has wide adaptability in Indian citriculture. Rough lemon and Volkamer lemon are comparable in horticultural performance and their resistance against biotic and abiotic stresses are almost similar but Volkamer lemon has better adaptability in different agroclimatic conditions. To improve Volkamer lemon hybridization has been attempted with Kinkoji (citrus greening tolerance), Swingle citrumelo (<em>Phytophthora</em> resistance), and Cleopatra (salinity tolerance). Polyembryony in Volkamer lemon and absence of the heritable morphological markers (Cleopatra) are limiting factors for hybrid identification and morphological characters are not being segregated in Kinkoji and Swingle citrumelo at early stage, then molecular markers are employed for detection of putative zygotic seedlings. We performed hybridization between Volkamer lemon (♀), Kinkoji (B cross) (58 F<sub>1</sub>) (♂), Swingle citrumelo (D cross) (71 F<sub>1</sub>) (♂), Cleopatra (N cross) (83 F<sub>1</sub>) (♂) and discriminate the hybrids by using 25 SSR markers. The fifteen markers were found efficient for hybrid identification (> 71% hybrids detected). CCSME23 (88.21%) marker has detected 187 hybrids in all crosses thereafter, 184 hybrids detected each by HvSSRCS-2, HvSSRCS-135, HvSSRCS-237, HvSSRCM-92 and HvSSRCM-466 with 86.79%. A total of 44 qualitative and 18 quantitative characters were analysed. Fifteen qualitative and six quantitative characters were found significant variability in leaf, spine, branch, and stem. Sufficient amount of genetic variability was detected through phylogenetic analysis and population structure analysis showed two subpopulations in B and N cross but in D cross five subpopulations were detected. After primary screening, nine F<sub>1</sub> hybrids for citrus greening tolerance and twelve F<sub>1</sub> hybrids for <em>Phytophthora</em> tolerance were identified. The newly developed F<sub>1</sub> hybrids exhibited phenotypic and genetic diversity along with disease tolerance. These potential hybrids may be recommended for commercial citriculture after thorough horticultural evaluation.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"5 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139668259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Composite edible coatings can extend shelf-life and maintain postharvest qualities of guava under natural storage","authors":"Karishma Kohli, Ankit Kumar, Omveer Singh, Prithwiraj Dey","doi":"10.1007/s13580-023-00576-1","DOIUrl":"https://doi.org/10.1007/s13580-023-00576-1","url":null,"abstract":"<p>The current study explores innovative strategies for extending the postharvest shelf life of guava under high humidity and temperature conditions prevalent in sub-tropical regions with composite edible coatings to guava fruits and analyzing their impact on key physiological parameters. Results reveal that the composite Olive oil with texture enhancer + Antioxidant (OTE + AO) treatment consistently maintained superior fruit firmness throughout storage, with a remarkable reduction in softening by 34.3% compared to uncoated controls. Composite edible coatings viz. Olive oil with texture enhancer (OTE), Sodium alginate with texture enhancer + Antioxidant (STE + AO), and Aloe vera with texture enhancer + Antioxidant (ATE + AO) exhibited elevated titratable acidity levels on the third day of storage, surpassing the control by 10–15%, indicating potential for extended shelf life. Moreover, coated fruits exhibited sustained total sugar levels, with OTE + AO treatment demonstrating a significant 8.5% increase compared to controls after fifteen days. Notably, the OTE + AO and OTE treatments showcased the least decline in total flavonoid content throughout storage, maintaining 12–15% higher levels than uncoated counterparts. These displayed substantial efficacy in mitigating polyphenol oxidase activity, reducing enzymatic browning by 32.7–34.3%. Preserving superoxide dismutase activity in coated treatments, resulting in 10–15% increased levels, indicates enhanced antioxidant defence systems. This study’s results have found the possibility of extending guava’s shelf life with optimum biochemical properties with composite coatings.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"237 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139649499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dongfang Shi, Boya Zhao, Pingping Zhang, Ping Li, Xinpei Wei, Kai Song
{"title":"Edible composite films: enhancing the postharvest preservation of blueberry","authors":"Dongfang Shi, Boya Zhao, Pingping Zhang, Ping Li, Xinpei Wei, Kai Song","doi":"10.1007/s13580-023-00581-4","DOIUrl":"https://doi.org/10.1007/s13580-023-00581-4","url":null,"abstract":"<p>Blueberry is popular among consumers for their unique flavor and rich nutritional content. However, fresh blueberry often has a short shelf life due to microbial contamination and water loss. Existing blueberry preservation methods, such as irradiation, air conditioning, and high-voltage electrostatic fields, have shown certain effectiveness. Nonetheless, their high cost and energy consumption limit their widespread use. Edible composite films, created by incorporating functional substances into an edible film matrix, represent an eco-friendly preservation technology. These films alter the surface microenvironment of fruits by establishing a selective barrier on the fruit's surface, which significantly enhances fruit storage quality. This article provides a comprehensive review of the application of edible composite films in blueberry preservation. By summarizing the spoilage process of blueberry during harvesting and storage, in conjunction with fruit preservation techniques, it explores the feasibility and potential applications of edible composite films. Lastly, it delves into the possibilities and challenges of integrating nanomaterials with edible composite films.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139668258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jeong So, Hak-Dong Lee, Ji Hyun Kim, Sanghyun Lee, Jin Hee Lim
{"title":"Antioxidant, antimicrobial, and skin-whitening effects and quantitative analysis of phenolic compounds in Korean wild Chrysanthemum flowers via HPLC/UV","authors":"Jeong So, Hak-Dong Lee, Ji Hyun Kim, Sanghyun Lee, Jin Hee Lim","doi":"10.1007/s13580-023-00570-7","DOIUrl":"https://doi.org/10.1007/s13580-023-00570-7","url":null,"abstract":"<p>To assess the potential of Korean wild <i>Chrysanthemum</i> flowers as cosmetic ingredients, this study evaluated the antioxidant, antimicrobial, and skin-whitening activities of extracts from <i>Chrysanthemum zawadskii</i> var. <i>latilobum</i>, <i>C. zawadskii</i> var. <i>lucidum</i>, <i>C. zawadskii</i> var. <i>yezoense</i>, and <i>C. makinoi</i>. Subsequently, HPLC analyses were conducted to characterize the phenolic compound contents of these extracts. The Korean wild <i>Chrysanthemum</i> flowers exhibited strong antioxidant properties, as evidenced by in vitro radical scavenging activity assays. They also exhibited skin-whitening activity by inhibiting melanin contents in α-melanocyte-stimulating hormone-induced B16F10 cells. Notably, <i>C. zawadskii</i> var. <i>lucidum</i> displayed higher antioxidant activity due to its elevated flavonoid content and potent radical scavenging activity compared to other wild <i>Chrysanthemum</i> flowers. Furthermore, <i>C. makinoi</i> exhibited strong in vitro tyrosinase inhibitory activity and reduced melanin contents in comparison to the other wild <i>Chrysanthemum</i> flowers. Our findings also confirmed the antimicrobial activity of the wild <i>Chrysanthemum</i> flower extracts against <i>Staphylococcus aureus, Pseudomonas aeruginosa,</i> and <i>Candida albicans</i>. Additionally, our HPLC analyses revealed that C. <i>zawadskii</i> var. <i>lucidum</i> had significantly higher contents of chlorogenic acid, linarin, 3,5-di-<i>O</i>-caffeoylquinic acid, and 4,5-di-<i>O</i>-caffeoylquinic acid. Therefore, Korean wild <i>Chrysanthemum</i> flowers hold promise as functional cosmetic ingredients due to their antioxidant, antimicrobial, and skin-whitening effects, as well as their elevated levels of phenolic compounds.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"299 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139649254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pharmacological effects of pentacyclic triterpenoids isolated from Centella asiatica","authors":"","doi":"10.1007/s13580-023-00561-8","DOIUrl":"https://doi.org/10.1007/s13580-023-00561-8","url":null,"abstract":"<h3>Abstract</h3> <p><em>Centella asiatica</em> (CA) is one of the most popular traditional herbal medicines worldwide. It has been used for centuries in many countries, especially for curing skin damage, and is now applied to treat various human diseases. There are various types of triterpenoids from <em>Centella asiatica</em>, with four pentacyclic triterpenoids with the main properties being shown by four pentacyclic triterpenoids: asiaticoside, madecassoside, asiatic acid, and madecassic acid. These terpenoids have similar structures, however each has a slightly different properties. Asiaticoside, madecassoside, asiatic acid, and madecassic acid are synthesized through the isoprenoid pathway known as mevalonate pathway to produce hydrophobic triterpenoid structures (aglycone) which contain hydrophilic sugar chains (glycone). Furthermore, asiaticoside and madecassoside are distinguished by a glycone, and asiatic acid and madecassic acid are distinguished by a aglycone. These pentacyclic triterpenoids have a wide spectrum of beneficial effects and have been used as anti-inflammatories, skin wound treatments, scar treatments, and cosmetics agents. This review aimed to provide a description of the four compounds, of their structure, pharmacological properties, applications in the treatment of various diseases, known mechanisms of action, and commentary on industrial applications.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":"299 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139649496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}