结合形态特征和微卫星标记对柑橘砧木杂交种进行鉴定和多样性分析

IF 2.4 3区 农林科学 Q1 Agricultural and Biological Sciences
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引用次数: 0

摘要

摘要 粗柠檬是印度本土品种,在印度柠檬栽培中具有广泛的适应性。粗柠檬和沃尔卡默柠檬的园艺表现相当,它们对生物和非生物胁迫的抗性几乎相似,但沃尔卡默柠檬对不同农业气候条件的适应性更强。为了改良沃尔卡默柠檬,人们尝试与 Kinkoji(耐柑橘绿化)、Swingle citrumelo(抗病虫害)和 Cleopatra(耐盐碱)杂交。沃尔卡默柠檬的多胚性和缺乏可遗传的形态标记(Cleopatra)是杂交鉴定的限制因素,而 Kinkoji 和 Swingle citrumelo 的形态特征在早期阶段并没有分离,因此采用分子标记来检测假定的合子苗。我们在 Volkamer 柠檬(♀)、Kinkoji(B 杂交)(58 F1)(♂)、Swingle citrumelo(D 杂交)(71 F1)(♂)、Cleopatra(N 杂交)(83 F1)(♂)之间进行了杂交,并利用 25 个 SSR 标记对杂交种进行了鉴别。结果发现,15 个标记能有效鉴定杂交种(71% 的杂交种被检出)。CCSME23(88.21%)标记在所有杂交中检测出 187 个杂交种,HvSSRCS-2、HvSSRCS-135、HvSSRCS-237、HvSSRCM-92 和 HvSSRCM-466 各检测出 184 个杂交种,检出率为 86.79%。共分析了 44 个定性特征和 18 个定量特征。在叶、刺、枝和茎中发现 15 个定性特征和 6 个定量特征存在显著变异。通过系统发育分析发现了足够的遗传变异,种群结构分析表明在 B 和 N 杂交中发现了两个亚群,但在 D 杂交中发现了五个亚群。经过初筛,确定了 9 个耐柑橘绿化病的 F1 代杂交种和 12 个耐疫霉病的 F1 代杂交种。新培育的 F1 代杂交种表现出表型和遗传多样性以及抗病性。经过全面的园艺评估后,这些潜在的杂交种可推荐用于商业柑橘栽培。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification and diversity analysis of interspecific citrus rootstock hybrids with combination of morphological traits and microsatellite markers

Abstract

Rough lemon is indigenous and has wide adaptability in Indian citriculture. Rough lemon and Volkamer lemon are comparable in horticultural performance and their resistance against biotic and abiotic stresses are almost similar but Volkamer lemon has better adaptability in different agroclimatic conditions. To improve Volkamer lemon hybridization has been attempted with Kinkoji (citrus greening tolerance), Swingle citrumelo (Phytophthora resistance), and Cleopatra (salinity tolerance). Polyembryony in Volkamer lemon and absence of the heritable morphological markers (Cleopatra) are limiting factors for hybrid identification and morphological characters are not being segregated in Kinkoji and Swingle citrumelo at early stage, then molecular markers are employed for detection of putative zygotic seedlings. We performed hybridization between Volkamer lemon (♀), Kinkoji (B cross) (58 F1) (♂), Swingle citrumelo (D cross) (71 F1) (♂), Cleopatra (N cross) (83 F1) (♂) and discriminate the hybrids by using 25 SSR markers. The fifteen markers were found efficient for hybrid identification (> 71% hybrids detected). CCSME23 (88.21%) marker has detected 187 hybrids in all crosses thereafter, 184 hybrids detected each by HvSSRCS-2, HvSSRCS-135, HvSSRCS-237, HvSSRCM-92 and HvSSRCM-466 with 86.79%. A total of 44 qualitative and 18 quantitative characters were analysed. Fifteen qualitative and six quantitative characters were found significant variability in leaf, spine, branch, and stem. Sufficient amount of genetic variability was detected through phylogenetic analysis and population structure analysis showed two subpopulations in B and N cross but in D cross five subpopulations were detected. After primary screening, nine F1 hybrids for citrus greening tolerance and twelve F1 hybrids for Phytophthora tolerance were identified. The newly developed F1 hybrids exhibited phenotypic and genetic diversity along with disease tolerance. These potential hybrids may be recommended for commercial citriculture after thorough horticultural evaluation.

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来源期刊
Horticulture Environment and Biotechnology
Horticulture Environment and Biotechnology Agricultural and Biological Sciences-Horticulture
CiteScore
4.30
自引率
4.20%
发文量
0
审稿时长
6 months
期刊介绍: Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science". HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI. The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.
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