Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University最新文献_第6页

[Infective pathogens and drug resistance in burned patients]. [烧伤患者感染病原菌及耐药情况]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Xi Zhang, Bo-cheng Zhao

{"title":"[Infective pathogens and drug resistance in burned patients].","authors":"Xi Zhang, Bo-cheng Zhao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To find out the changes in the infective pathogens and their drug resistance in burned patients.Methods: The patients were divided into two groups. The first group was from July 1991 to June 1996, and the second group was from July 1996 to June 2001. The bacteria of burned body surface and blood were cultured, and the bacteria and their drug sensitivity were analyzed.Results: Gram-negative bacteria were the major bacteria in burn infection, among which Pseudomonas aeruginosa ranked the top. Staphylococcus aureous ranked the first among the Gram-positive bacteria, and the isolation rate of methicillin resistant Staphylococcus aureus increased; The isolation rate of Enterobacter cloacae (10.4%), Escherichia coli (8.3%), Klebsiella pneumoniae (7.3%), and fungus (4.2%) all rose. The antibiotic resistant strains of Pseudomonas aeruginosa and Staphylococcus aureous increased.Conclusion: The changes in pathogens of burn infection and bacterial drug resistance are related to the wide use of broad spectrum antibiotics such as cefazidime and imepenem, suggesting that dynamic observation of changes in pathogenic strains and sensitivity of bacteria to antibiotics are useful for clinical prevention and cure of burn infection.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"405-8"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24106458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Competitive RT-PCR assay for quantification of GPI-PLD gene expression]. [竞争性RT-PCR法定量GPI-PLD基因表达]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Xiao-jie Zhang, Chun-ping Lu, Jian-hua Tang

{"title":"[Competitive RT-PCR assay for quantification of GPI-PLD gene expression].","authors":"Xiao-jie Zhang, Chun-ping Lu, Jian-hua Tang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To establish a competitive reverse transcription-polymerase chain reaction (RT-PCR) assay to quantify the expression of glycosylphosphatidylinositol specific phospholipase D (GPI-PLD) gene.Methods: A competitive GPI-PLD cDNA fragment was constructed by site-directed mutagenesis by PCR method. The competitive cDNA fragment had the same primer binding sites as the target cDNA, but was 20 bp shorter in length. The competitive cDNA was transcripted into the competitive RNA in vitro as the internal standard. Then the competitive RNA was reversely transcripted and amplified together with total RNA extracted from K562 cells. The two amplified cDNA fragments could be distinguished by 10% polyacrylamide gel electrophoresis. The concentration of cellular GPI-PLD mRNA was derived from the ratio between the intensities of the bands corresponding to the amplified products.Results: A 198 bp GPI-PLD competitive RNA was constructed and prepared. The competitive RNA could compete well with the target RNA in the RT-PCR reaction. The expression of GPI-PLD gene in K562 cells was (440 +/- 20) copies/ng.Conclusion: A competitive RT-PCR assay for the quantification of GPI-PLD gene expression may be established successfully. This method is highly sensitive, specific, and accurate.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"322-6"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24105584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Expression of S100 in oral submucose fibrosis]. S100在口腔黏膜下纤维化中的表达。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Xiao-li Xie, Zhan-gui Tang, Zhi-gang Yao

引用次数: 0

[Surgical treatment of 92 cases of thoracic aortic aneurysms]. [92例胸主动脉瘤的外科治疗]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Guo-bao Song, Xin-min Zhou, Jian-guo Hu

{"title":"[Surgical treatment of 92 cases of thoracic aortic aneurysms].","authors":"Guo-bao Song, Xin-min Zhou, Jian-guo Hu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To explore the timing of surgical treatment of thoracic aortic aneurysms.Methods: The clinical data of 92 patients with thoracic aortic aneurysm who underwent surgical treatment in our department during the latest 10 years were retrospectively reviewed.Results: The overall operative mortality was 13.04% (12/92) 30 days after the operation. Risk factors for the early mortality were the preoperative aneurysm breach, hypertension, important organ dysfunction and circulatory arrest during the operation, as well as the serious postoperative complications, such as arrhythmia, dysfunction or failure and infection of important organ, and so on (P < 0.05-0.01).Conclusion: The surgical treatment is an ideal way of treatment of thoracic aortic aneurysm. To choose appropriate timing and methods for the operation, to improve skills of the operation, to shorten the time of the operation, and to protect function of important organs contribute to the increase of the successful rate of the surgical treatment of thoracic aortic aneurysm and the decrease of the incident of postoperative complications.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"391-4"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24106454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Clinical observation of recombinant growth hormone therapy for liver cirrhosis with hypoproteinemia]. 重组生长激素治疗肝硬化合并低蛋白血症的临床观察

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Shao-jun Liu, Wu-liang Tang, Shou-rong Shen

引用次数: 0

[Effect of Coptis Chinensis compound on the gene expression in transplanted tumor tissue in nasopharyngeal carcinoma cell line of CNE1 by cDNA microarray]. [利用cDNA芯片研究黄连复方对鼻咽癌CNE1细胞系移植瘤组织中基因表达的影响]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Guang-ping Wang, Fa-qing Tang, Jin-ping Zhou

{"title":"[Effect of Coptis Chinensis compound on the gene expression in transplanted tumor tissue in nasopharyngeal carcinoma cell line of CNE1 by cDNA microarray].","authors":"Guang-ping Wang, Fa-qing Tang, Jin-ping Zhou","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the effect of Coptis Chinensis compound on the gene expression of transplanted tumor in nasopharyngeal carcinoma cell line of CNE1.Methods: The cells were injected into Balb/C nude mice, and after the transplanted tumor was established, the nude mice with tumors were treated with Coptis chinensis compound. The nude mice were then killed, tumor tissues were isolated and the total RNA was extracted. Hybridization with a cDNA microarray was conducted using a probe of fluorescent-labeled cDNA mixture that was reversely transcribed from the RNA. The gene expression level was analyzed after scanning the fluorescent intensity by a laser scanner, and further confirmed by RT-PCR.Results: After using Coptis Chinensis compound for 30 days, the size of transplanted tumors were remarkably reduced, and the inhibition rate of tumor growth was 29.5%. In the meantime, the expression of 147 genes in the tissue of implanted tumor treated with the compound altered: the expression of 102 genes was down-regulated, and that of 45 genes was up-regulated. Furthermore, the differential expression of 3 genes was verified by RT-PCR, MAD3 and H731 genes were up-regulated, and that of CHK1 was down-regulated.Conclusion: Coptis Chinensis compound can affect the gene expression in the tissue of implanted tumor, which implies that the inhibition of Coptis Chinensis compound on the growth of the implanted tumor with CNE1 cells in nude mice might be associated with the control of gene expression.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"347-52"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24104925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Clinical evaluation of hypotonic and hypopressive intravenous pyelography]. 静脉肾盂造影低渗和低压的临床评价。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Zuo-hua Li, Xi-long Mei, En-hua Xiao

引用次数: 0

[Expression of ET-3 in hippocampal CA1 of ovariectomied mice after cerebral ischemia/reperfusion]. [去卵巢小鼠脑缺血/再灌注后海马CA1中ET-3的表达]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Zhi-jun Zhang, Zheng-qing Liu, Zhi-jian Ma

{"title":"[Expression of ET-3 in hippocampal CA1 of ovariectomied mice after cerebral ischemia/reperfusion].","authors":"Zhi-jun Zhang, Zheng-qing Liu, Zhi-jian Ma","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To detect the expression of endothelins-3 (ET-3) immunoactivity in hippocampal CA1 of ovariectomied (OVX) mice after ischemia/reperfusion (IR), and to further explore the relationship between estrogen and ET-3.Methods: Fifty-four adult female mice were randomly divided into 3 groups. 1. IR group: the bilateral common carotid occlusion (BCCO) was carried out for 7 min on the 1st, 3rd, 5th and 14th day of reperfusion. 2. OVX/IR group: firstly the ovariectomy was performed, and after a week, BCCO was carried out for 7 min on the 1st, 3rd, 5th and 14th day of reperfusion (each time spot, n = 6). 3. sham-operated group (n = 6).Results: Few ET-3 positive cells were diffusely distributed in the hippocampal CA1 region in the control group, whereas the expression of ET-3 increased in the experimental groups. The number of ET-3 positive cells significantly increased in the OVX/IR group compared with that of the IR group 1 d after the reperfusion (P < 0.01). It reached the maximum in both the OVX/IR and IR group on the 5th day of the reperfusion.Conclusion: Estrogen is involved in the regulation of ET-3 in the early stage of the reperfusion, and probably plays a protective role in the survival of neurons.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"371-4"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24106032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Clinical characteristics and genetic analysis of rare chromosome aberration in peripheral blood]. 外周血罕见染色体畸变的临床特点及遗传学分析

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Sheng-xiang Chen, Li Liu, Han-wen Wu

引用次数: 0

[Ambulatory pulse pressure and left ventricular hypertrophy in patients with essential hypertension]. [原发性高血压患者动态脉压与左心室肥厚]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Yang Cao, Shui-ping Zhao, Lin Luo

引用次数: 0