Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University最新文献

[Clinical analysis of cervical lymph node metastasis as primary manifestation of oral cancer]. 【口腔癌以颈淋巴结转移为主的临床分析】。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Can-hua Jiang, Xin-chun Jian, Hong-quan Cheng

引用次数: 0

[Serum soluble CD40L level and its application in coronary heart disease]. [血清可溶性CD40L水平及其在冠心病中的应用]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Jiang Li, Dao-quan Peng, Shui-ping Zhao

{"title":"[Serum soluble CD40L level and its application in coronary heart disease].","authors":"Jiang Li, Dao-quan Peng, Shui-ping Zhao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the serum level of soluble CD40 ligand (sCD40L) in coronary heart disease and its relationship with serum lipid levels.Methods: Enzyme-linked immunosorbent (EIA) was used to measure sCD40L in 129 patients with coronary heart disease (46 with acute myocardial infarction, 37 with unstable angina pectoris and the other 46 with stable angina pectoris) and 30 matched healthy controls.Results: sCD40L level was significantly higher in patients with acute coronary syndrome (ACS) [(2. 99 +/- 2.19), (2.23 +/- 0.67) ng/ml in acute myocardial infarction and unstable angina pectoris group, respectively] than that of those with stable coronary heart disease and that of controls [(1.12 +/- 0.42) and (0.89 +/- 0.55) ng/ml, respectively] (P < 0.05). Serum sCD40L level was significantly positively related to ApoB100 (r = 0.159, P = 0.046), and negatively related to HDL-C (r = -0.192, P = 0.015).Conclusion: The levels of sCD40L increase in acute coronary syndrome, which may be related to the pathogenesis of ACS, and can be used as a potential marker of plaque instability.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 6","pages":"611-4"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25036388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Indomethacin-induced HL-60 leukemic cell apoptosis and the activation of C-jun NH2- terminal kinase signal transduction pathway]. [吲哚美辛诱导HL-60白血病细胞凋亡及C-jun NH2末端激酶信号转导通路的激活]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Guang-sen Zhang, Ding-sheng Liu, Meng Xia

{"title":"[Indomethacin-induced HL-60 leukemic cell apoptosis and the activation of C-jun NH2- terminal kinase signal transduction pathway].","authors":"Guang-sen Zhang, Ding-sheng Liu, Meng Xia","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To observe the effect of indomethacin on inhibiting the proliferation of HL-60 cells and inducing HL-60 cells apoptosis and to explore the activation of C-Jun NH2-terminal kinase (JNK) signal transduction pathway in mediating indomethacin-induced HL-60 cell apoptosis.Methods: The cell viability was determined by Trypan blue staining and the state of cell proliferation was analyzed; DNA ladder pattern and AO/EB staining were applied to identify the cell apoptosis; the apoptotic signal proteins including caspase-9, caspase-3, and PARP and the proteins of JNK signal pathway such as MEK4, JNK, P-JNK, and P-C-Jun, were detected by Western blotting.Results: Indomethacin at 200 to approximately 400 micromol significantly inhibited the proliferation of HL-60 cells and induced the cell apoptosis in a time- or concentration-dependent manner; caspase-9, caspase-3, and PARP were cleaved and activated in undergoing apoptotic cells; and the expressions of MEK4, P-JNK, and P-C-Jun were upregulated with the increase of indomethacin concentration.Conclusion: Indomethacin can inhibit the proliferation of HL-60 cell and induce leukemic cell apoptosis. The activation of JNK signal transduction pathway mediates the event of indomethacin-induced HL-60 cell apoptosis. JNK signal pathway is located in the upstream of caspase signal pathway.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 6","pages":"557-62"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25206885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Technique for undecalcified bone tissue. Immunohistochemistry and in situ hybridization]. 未钙化骨组织的技术。免疫组织化学和原位杂交]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Jian Li, Er-yuan Liao, Qi-you Wei

{"title":"[Technique for undecalcified bone tissue. Immunohistochemistry and in situ hybridization].","authors":"Jian Li, Er-yuan Liao, Qi-you Wei","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the feasibility of applying immunohistochemistry (IH) and in situ hybridization (ISH) technique to the undecalcified bone sections.Methods: The proximal tibiae of SD rats were embedded with the modified methylmethacrylate low-temperature embedding method and then made into undecalcified bone sections. The sections were stained with Goldner's Masson Trichrome, and the expression of IGF-1 mRNA and protein in the bone tissue cells were detected by IH and ISH.Results: Goldner's staining demonstrated the structure of trabeculae bone was intact and osteoid at the edge of trabeculae stained red. Osteoblasts and osteoclasts in bone sections could be seen clearly. The positive signals of IGF-1 mRNA and protein were found in the cytoplasm of osteoblasts, chondrocytes in epiphyseal plate and some mononuclear cells in the bone marrow. The matrix of trabeculae also showed positive expression of IGF-1 protein.Conclusion: The undecalcified bone sections prepared by the method can meet the needs of IH and ISH. The establishment of this method will provide technological platform for the study of molecular pathology on metabolic bone diseases such as osteoporosis.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 6","pages":"601-4"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25036385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Preservation of the entire mitral subvalvular apparatus during mitral valve replacement in patients with mitral stenosis]. [二尖瓣狭窄患者在二尖瓣置换术中保存整个二尖瓣下器官]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Sheng-xi Chen, Er-ping Xi, Wei-xing Zhang

{"title":"[Preservation of the entire mitral subvalvular apparatus during mitral valve replacement in patients with mitral stenosis].","authors":"Sheng-xi Chen, Er-ping Xi, Wei-xing Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To evaluate the surgical technique and the effect of preservation of the entire mitral subvalvular apparatus during mitral valve replacement (MVR) in patients with mitral stenosis.Methods: MVR was performed on 56 patients with mitral stenosis. Of them, 11 had complete preservation of the entire mitral subvalvular apparatus (group A) with the technique of preservation of 'button-shaped' transplantation, 25 had the preservation of the posterior leaflet only (group B), and the other 20 underwent the conventional MVR (group C). Pre- and postoperative hemodynamic parameters were measured to determine the left ventricular performance. Echocardiography was performed preoperatively, at the time of discharge, and 3 to approximately 6 months postoperatively to determine the dimensions and ejection function.Results: Cardiac index, stroke volume index and left ventricular stroke work index postoperatively in group A were better than those in group B and C (P < 0.05). Echocardiographic measurements postoperatively showed the increase of LVESD, LVEDD in group B and C, but a better LVL and LVFS in group A (P < 0.05), and LVEF was more pronounced in group A (P < 0.05). There appeared a long cross-clamp time in group A, but the total CPB time was not significant among the three groups.Conclusion: The surgical skills of preservation with 'button-shaped' transplantation may be a practical choice for patients with mitral stenosis. The preservation of entire mitral subvalvular apparatus improves the left ventricular functions after MVR in patients with mitral valve stenosis.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 6","pages":"615-8"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25036389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effect of rocuronium on hemodynamic in patients undergoing cardiac valve replacement]. 罗库溴铵对心脏瓣膜置换术患者血流动力学的影响。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

You-qing Li, Fei Ren, Hong-wei Yang

引用次数: 0

[Serum levels of antibodies for IgG, IgA, and IgM against the fungi antigen in psoriasis vulgaris]. [寻常型银屑病血清抗真菌抗原IgG、IgA和IgM抗体水平]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Yun-sheng Liang, Hai-quan Wen, Rong Xiao

引用次数: 0

[Etiologic analysis of 1137 inpatients of chronic renal failure]. [1137例住院慢性肾功能衰竭的病因分析]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Xiao-xia Du, Hong Liu, You-Ming Peng

引用次数: 0

[Significance and plasma level of PAI-1 on the angiographic severity of coronary lesions and coronary event]. [血浆PAI-1水平对冠脉造影病变严重程度及冠脉事件的意义]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Hai-tao Yang, Shu-shan Qi, Xiang-qian Shen

引用次数: 0

[Expression and purification of human metallothionein-1E gene in Escherichia coli]. 人金属硫蛋白1e基因在大肠杆菌中的表达与纯化

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Fang Yang, Zhi-min He, Ying Sun

引用次数: 0