Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University最新文献

[Clinical analysis of cervical lymph node metastasis as primary manifestation of oral cancer]. 【口腔癌以颈淋巴结转移为主的临床分析】。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Can-hua Jiang, Xin-chun Jian, Hong-quan Cheng

引用次数: 0

[Serum soluble CD40L level and its application in coronary heart disease]. [血清可溶性CD40L水平及其在冠心病中的应用]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Jiang Li, Dao-quan Peng, Shui-ping Zhao

{"title":"[Serum soluble CD40L level and its application in coronary heart disease].","authors":"Jiang Li, Dao-quan Peng, Shui-ping Zhao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the serum level of soluble CD40 ligand (sCD40L) in coronary heart disease and its relationship with serum lipid levels.Methods: Enzyme-linked immunosorbent (EIA) was used to measure sCD40L in 129 patients with coronary heart disease (46 with acute myocardial infarction, 37 with unstable angina pectoris and the other 46 with stable angina pectoris) and 30 matched healthy controls.Results: sCD40L level was significantly higher in patients with acute coronary syndrome (ACS) [(2. 99 +/- 2.19), (2.23 +/- 0.67) ng/ml in acute myocardial infarction and unstable angina pectoris group, respectively] than that of those with stable coronary heart disease and that of controls [(1.12 +/- 0.42) and (0.89 +/- 0.55) ng/ml, respectively] (P < 0.05). Serum sCD40L level was significantly positively related to ApoB100 (r = 0.159, P = 0.046), and negatively related to HDL-C (r = -0.192, P = 0.015).Conclusion: The levels of sCD40L increase in acute coronary syndrome, which may be related to the pathogenesis of ACS, and can be used as a potential marker of plaque instability.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 6","pages":"611-4"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25036388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Indomethacin-induced HL-60 leukemic cell apoptosis and the activation of C-jun NH2- terminal kinase signal transduction pathway]. [吲哚美辛诱导HL-60白血病细胞凋亡及C-jun NH2末端激酶信号转导通路的激活]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Guang-sen Zhang, Ding-sheng Liu, Meng Xia

{"title":"[Indomethacin-induced HL-60 leukemic cell apoptosis and the activation of C-jun NH2- terminal kinase signal transduction pathway].","authors":"Guang-sen Zhang, Ding-sheng Liu, Meng Xia","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To observe the effect of indomethacin on inhibiting the proliferation of HL-60 cells and inducing HL-60 cells apoptosis and to explore the activation of C-Jun NH2-terminal kinase (JNK) signal transduction pathway in mediating indomethacin-induced HL-60 cell apoptosis.Methods: The cell viability was determined by Trypan blue staining and the state of cell proliferation was analyzed; DNA ladder pattern and AO/EB staining were applied to identify the cell apoptosis; the apoptotic signal proteins including caspase-9, caspase-3, and PARP and the proteins of JNK signal pathway such as MEK4, JNK, P-JNK, and P-C-Jun, were detected by Western blotting.Results: Indomethacin at 200 to approximately 400 micromol significantly inhibited the proliferation of HL-60 cells and induced the cell apoptosis in a time- or concentration-dependent manner; caspase-9, caspase-3, and PARP were cleaved and activated in undergoing apoptotic cells; and the expressions of MEK4, P-JNK, and P-C-Jun were upregulated with the increase of indomethacin concentration.Conclusion: Indomethacin can inhibit the proliferation of HL-60 cell and induce leukemic cell apoptosis. The activation of JNK signal transduction pathway mediates the event of indomethacin-induced HL-60 cell apoptosis. JNK signal pathway is located in the upstream of caspase signal pathway.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 6","pages":"557-62"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25206885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Technique for undecalcified bone tissue. Immunohistochemistry and in situ hybridization]. 未钙化骨组织的技术。免疫组织化学和原位杂交]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Jian Li, Er-yuan Liao, Qi-you Wei

{"title":"[Technique for undecalcified bone tissue. Immunohistochemistry and in situ hybridization].","authors":"Jian Li, Er-yuan Liao, Qi-you Wei","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the feasibility of applying immunohistochemistry (IH) and in situ hybridization (ISH) technique to the undecalcified bone sections.Methods: The proximal tibiae of SD rats were embedded with the modified methylmethacrylate low-temperature embedding method and then made into undecalcified bone sections. The sections were stained with Goldner's Masson Trichrome, and the expression of IGF-1 mRNA and protein in the bone tissue cells were detected by IH and ISH.Results: Goldner's staining demonstrated the structure of trabeculae bone was intact and osteoid at the edge of trabeculae stained red. Osteoblasts and osteoclasts in bone sections could be seen clearly. The positive signals of IGF-1 mRNA and protein were found in the cytoplasm of osteoblasts, chondrocytes in epiphyseal plate and some mononuclear cells in the bone marrow. The matrix of trabeculae also showed positive expression of IGF-1 protein.Conclusion: The undecalcified bone sections prepared by the method can meet the needs of IH and ISH. The establishment of this method will provide technological platform for the study of molecular pathology on metabolic bone diseases such as osteoporosis.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 6","pages":"601-4"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25036385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Preservation of the entire mitral subvalvular apparatus during mitral valve replacement in patients with mitral stenosis]. [二尖瓣狭窄患者在二尖瓣置换术中保存整个二尖瓣下器官]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Sheng-xi Chen, Er-ping Xi, Wei-xing Zhang

{"title":"[Preservation of the entire mitral subvalvular apparatus during mitral valve replacement in patients with mitral stenosis].","authors":"Sheng-xi Chen, Er-ping Xi, Wei-xing Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To evaluate the surgical technique and the effect of preservation of the entire mitral subvalvular apparatus during mitral valve replacement (MVR) in patients with mitral stenosis.Methods: MVR was performed on 56 patients with mitral stenosis. Of them, 11 had complete preservation of the entire mitral subvalvular apparatus (group A) with the technique of preservation of 'button-shaped' transplantation, 25 had the preservation of the posterior leaflet only (group B), and the other 20 underwent the conventional MVR (group C). Pre- and postoperative hemodynamic parameters were measured to determine the left ventricular performance. Echocardiography was performed preoperatively, at the time of discharge, and 3 to approximately 6 months postoperatively to determine the dimensions and ejection function.Results: Cardiac index, stroke volume index and left ventricular stroke work index postoperatively in group A were better than those in group B and C (P < 0.05). Echocardiographic measurements postoperatively showed the increase of LVESD, LVEDD in group B and C, but a better LVL and LVFS in group A (P < 0.05), and LVEF was more pronounced in group A (P < 0.05). There appeared a long cross-clamp time in group A, but the total CPB time was not significant among the three groups.Conclusion: The surgical skills of preservation with 'button-shaped' transplantation may be a practical choice for patients with mitral stenosis. The preservation of entire mitral subvalvular apparatus improves the left ventricular functions after MVR in patients with mitral valve stenosis.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 6","pages":"615-8"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25036389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effect of rocuronium on hemodynamic in patients undergoing cardiac valve replacement]. 罗库溴铵对心脏瓣膜置换术患者血流动力学的影响。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

You-qing Li, Fei Ren, Hong-wei Yang

引用次数: 0

[Establishment and characteristics of the human diploid cell strain HLF-02]. [人二倍体细胞株HLF-02的建立及特性研究]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Bai-ying Huang, Ming Li, Shang-Hhi Wu

{"title":"[Establishment and characteristics of the human diploid cell strain HLF-02].","authors":"Bai-ying Huang, Ming Li, Shang-Hhi Wu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To establish the HLF-02 and provide a new material to the study of geronics, oncology, virology, pharmacology, and biogenetics.Methods: The lung tissue was digested with 0.15% trypsin. More than 90% of the dissociated cell exhibited initial viability (trypan blue dye exclusion and the ability of adhesion). The surviving cells were analyzed by morphology, growth curves, karyotype analysis, electron microscopic observation and heterotransplantation.Results: A newly established cell strain, which was designated HLF-02 had been maintained in continuous culture for over 50 generations for 10 months. Morphological observation, electron microscopic observation, karyotype analysis and heterotransplantation showed that it was corresponding to diploid cells. The doubling time of the cells was 75.5 hours. Chromosome analyses showed the human cell strain to be typical diploid appearance, and the tumorigenecity assays of nude mice were negative.Conclusion: The HLF-02 is a human dipolid cell strain and can serve the further studies of cell gerontics, oncology, virology, pharmacology, and biogenetics.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 6","pages":"587-90"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25206226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Expression and purification of human metallothionein-1E gene in Escherichia coli]. 人金属硫蛋白1e基因在大肠杆菌中的表达与纯化

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Fang Yang, Zhi-min He, Ying Sun

引用次数: 0

[Effects of nitric oxide on form-deprivation myopia in chicks]. 一氧化氮对雏鸡形态剥夺性近视的影响。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Yu-guang Zhu, Shuang-zhen Liu, Xiao-ying Wu

{"title":"[Effects of nitric oxide on form-deprivation myopia in chicks].","authors":"Yu-guang Zhu, Shuang-zhen Liu, Xiao-ying Wu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To construct a chick model of form-deprivation myopia (FDM) to investigate the effect of nitric oxide (NO) on FDM.Methods: FDM was induced in 60 one-day white leghorn chicks. They were divided into group A and B. One eye was covered randomly, and the other served as control. In each week of the experimental period, the retinoscopic refraction was performed, and the axial length was measured with A-mode ultrasound. The NO concentration of retina and choroid from the control and the experimental eyes was measured using NO detection kit. Change in the expression of iNOS mRNA was measured by RT-PCR. The activity of iNOS was analyzed with immunohistochemistry.Results: Form-deprivation eyes were significantly more myopic than the controls, and the axial length of occluded eyes was significantly longer than that of the controls. Unocclusion attenuated the development of myopia and inhibited the axial elongation. Form-deprivation reduced the NO concentration ,the immunoreactivity of iNOS, and the expression of iNOS mRNA. The gradual restoration of normal vision was found after uncovering the eyes. But the expression of iNOS mRNA was significantly higher than that of the controls at the first week after the unocclusion.Conclusion: Form-deprivation can construct an animal model of myopia. FDM is reversible before the chicks were mature. NO may be involved in the form of FDM.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 6","pages":"631-4"},"PeriodicalIF":0.0,"publicationDate":"2003-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25035118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Significance and plasma level of PAI-1 on the angiographic severity of coronary lesions and coronary event]. [血浆PAI-1水平对冠脉造影病变严重程度及冠脉事件的意义]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-12-01

Hai-tao Yang, Shu-shan Qi, Xiang-qian Shen

引用次数: 0