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Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University最新文献

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[Mechanical ventilation of acute exacerbated chronic obstructive pulmonary disease with CO2 retention]. 急性加重慢性阻塞性肺疾病伴CO2潴留的机械通气
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01
Min-shi Yang, Yue Peng, Wei Xing
{"title":"[Mechanical ventilation of acute exacerbated chronic obstructive pulmonary disease with CO2 retention].","authors":"Min-shi Yang, Yue Peng, Wei Xing","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"429-30"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24105874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Protective effect of Polygonum multiflorum thunb on the cerebral cholinergic neurofibers in rats]. 何首乌对大鼠脑胆碱能神经纤维的保护作用。
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01
Min Li, Xiao-ping Du, Hui Ye
{"title":"[Protective effect of Polygonum multiflorum thunb on the cerebral cholinergic neurofibers in rats].","authors":"Min Li,&nbsp;Xiao-ping Du,&nbsp;Hui Ye","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To observe the number and morphological changes of cholinergic neurofibers in rats under the destruction of kainic acid, and to investigate the protective effect and the mechanism of polygonum multiflorum thunb (PMT) on acetyl-chorine esterase (AChE) neurofibers.</p><p><strong>Methods: </strong>Excitative neurotoxin kainic acid was injected into the basal forebrain, Meynert neucleus, medial septal neucleus and the Broca neucleus to establish the destruction model. Then the destructive experimental group was fed with PMT, and the histochemical method was used to display the changes of AChE fibers, the protection and activation of PMT.</p><p><strong>Results: </strong>The number of projecting AChE fibers to the cortex and the hippocampus in PMT group was larger than that in the control group, and no morphological destruction was seen in the experimental group (P < 0.01).</p><p><strong>Conclusion: </strong>PMT has a protective effect on AchE projecting fibers in rats.</p>","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"361-4"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24106029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A case of Kallmann syndrome]. [1例Kallmann综合征]。
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01
Wang-yuan Zou, Jing Wu, Qu-lian Guo
{"title":"[A case of Kallmann syndrome].","authors":"Wang-yuan Zou,&nbsp;Jing Wu,&nbsp;Qu-lian Guo","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"408, 414"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24106459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Dry chemistry method for measuring serum urea and creatinine]. 【干化学法测定血清尿素和肌酐】。
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01
Zheng-yong Zhong, Li-na Yang, Guo-feng Luo
{"title":"[Dry chemistry method for measuring serum urea and creatinine].","authors":"Zheng-yong Zhong,&nbsp;Li-na Yang,&nbsp;Guo-feng Luo","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the dry chemistry method for measuring serum urea and creatinine.</p><p><strong>Methods: </strong>Urea and Creatinine were measured by dry and wet chemistry methods respectively. The results and the relativity between the two methods were compared.</p><p><strong>Results: </strong>The correlative factors were 0.996 and 0.995, respectively, implying fairly good comparability. The precision of the dry chemistry method was good, with the coefficient variation (CV) below 5%. With favorable accuracy, the recovery rate were 106.7% and 107.2%, respectively.</p><p><strong>Conclusion: </strong>Dry chemistry method is rapid and accurate for emergent testing of serum urea and creatinine.</p>","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"418-20"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24105870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Influence of rosiglitazone on the airway inflammation in guinea pigs with asthma]. [罗格列酮对哮喘豚鼠气道炎症的影响]。
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01
Zhi-jian Bao, Xu-dong Xiang, Jun-wei Deng
{"title":"[Influence of rosiglitazone on the airway inflammation in guinea pigs with asthma].","authors":"Zhi-jian Bao,&nbsp;Xu-dong Xiang,&nbsp;Jun-wei Deng","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"424-6, 436"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24105872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Nuclear factor-kappa B activity at the early stage of brain ischemia and reperfusion in mice]. [小鼠脑缺血再灌注早期核因子κ B的活性]。
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01
Jin-xiu Li, Jun-wei Deng, Guang-sen Zhang
{"title":"[Nuclear factor-kappa B activity at the early stage of brain ischemia and reperfusion in mice].","authors":"Jin-xiu Li,&nbsp;Jun-wei Deng,&nbsp;Guang-sen Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To observe the activity of nuclear factor-kappa B(NF-kappa B) during reperfusion after temporary brain ischemia and to evaluate its effect.</p><p><strong>Methods: </strong>The brain ischemia-reperfusion(I/R) model of mice was established with ligating bilateral common carotid arteries and bleeding for 0.3 ml in the tail. Abnormal nervous symptoms (ANS) were recorded. Immunohistochemical technique was used to detect the activity of NF-kappa B subunit P65 and the inhibitory factor-kappa B alpha(I-kappa B alpha) in cerebral cortex during different periods of ischemia and reperfusion. Their mRNA expressions were also measured by RT-PCR.</p><p><strong>Results: </strong>NF-kappa B P65 activity significantly increased 30 minutes after the reperfusion, peaking at the 2nd hour, and remaining high within 24 hours (all P < 0.05). But the mRNA expression didn't change much; I-kappa B alpha and its mRNA expression began to decrease at 30 minutes after the reperfusion, peaking at the 2nd hour after the reperfusion (P < 0.05) and then gradually restored. Positive correlation was found between NF-kappa B P65 activity and ANS (P < 0.05), while negative correlation was shown among I-kappa B alpha, its mRNA, and ANS (P < 0.05, respectively).</p><p><strong>Conclusion: </strong>Both activated NF-kappa B and decreased I-kappa B alpha exist in the neural tissues of mice at the early stage of brain ischemia-reperfusion. The activated NF-kappa B may be involved in the ischemia-reperfusion injury.</p>","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"365-70"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24106030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Amplification of beta-catenin gene in the paraffin-embedded epidermal tissue]. [石蜡包埋表皮组织β -连环蛋白基因扩增]。
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01
Xiang Chen, Hong-fu Xie, Fu-wen Chen
{"title":"[Amplification of beta-catenin gene in the paraffin-embedded epidermal tissue].","authors":"Xiang Chen,&nbsp;Hong-fu Xie,&nbsp;Fu-wen Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To establish a method of extracting DNA from the paraffin-embedded epidermal tissue and examine the amplification of beta-catenin gene by polymerase chain reaction (PCR) analysis.</p><p><strong>Methods: </strong>Exon 3 of the beta-catenin gene was amplified by PCR in 14 paraffin-embedded Bowen's disease (BD) of various weight and years.</p><p><strong>Results: </strong>The amplification of exon 3 of beta-catenin gene was successful in all the 10 mg and 20 mg samples, but not in the 5 mg samples. The amplification of exon 3 of beta-catenin gene was successful in 9 samples embedded within 10 years, and in only 2 of the 5 samples embedded more than 10 years.</p><p><strong>Conclusion: </strong>Epidermal tissue whose weight is 10 mg or more within 10 years of embedding can be used in the amplification of purpose gene by PCR.</p>","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"327-9"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24104920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Detection of single-nucleotide polymorphisms in insulin receptor substrate-2 gene 3'-untranslated region by denaturing high-performance liquid chromatography]. 【变性高效液相色谱法检测胰岛素受体底物2基因3′-非翻译区单核苷酸多态性】。
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01
Wei-min Zeng, Shu-hua Chen, Ping Xie
{"title":"[Detection of single-nucleotide polymorphisms in insulin receptor substrate-2 gene 3'-untranslated region by denaturing high-performance liquid chromatography].","authors":"Wei-min Zeng,&nbsp;Shu-hua Chen,&nbsp;Ping Xie","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To explore the use of denaturing high-performance liquid chromatography (DHPLC) in detecting single-nucleotide polymorphisms(SNPs) of insulin receptor substrate-2(IRS-2) gene 3'-untranslated region (3'-UTR).</p><p><strong>Methods: </strong>We detected the SNPs and mutation of IRS-2 gene 3'-UTR sequence, with polymerase chain reaction (PCR), DHPLC, single-strand conformation polymorphism (SSCP), and DNA sequence analysis respectively in 30 Type 2 diabetic subjects and 30 healthy controls.</p><p><strong>Results: </strong>The SNPs of IRS-2 gene 3'-UTR in 4 patients with Type 2 diabetes mellitus were detected by PCR-DHPLC and were identified by DNA sequencing.</p><p><strong>Conclusion: </strong>DHPLC is a rapid and automated technology, which is simpler and more accurate for detecting longer fragment of DNA than SSCP. The present method lays a foundation for further studying the relationship between the mutation of IRS-2 gene 3'-UTR and Type 2 diabetes mellitus.</p>","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"317-21"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24105583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Expression of MMP-9 and CD34 in papillary thyroid carcinomas]. [MMP-9和CD34在甲状腺乳头状癌中的表达]。
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01
Hong-bo Zhang, Qi-you Wei
{"title":"[Expression of MMP-9 and CD34 in papillary thyroid carcinomas].","authors":"Hong-bo Zhang,&nbsp;Qi-you Wei","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"427-8"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24105873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Dynamic observation of the arteriovenous fistula and the blood flow in patients undergoing hemodialysis]. 血液透析患者动静脉瘘与血流的动态观察。
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01
Jun Li, Hong Liu, Xiao-ping Zhu
{"title":"[Dynamic observation of the arteriovenous fistula and the blood flow in patients undergoing hemodialysis].","authors":"Jun Li,&nbsp;Hong Liu,&nbsp;Xiao-ping Zhu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"433-4"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24105876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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