Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University最新文献_第8页

[Effects of isoket on hemodynamics during extubation and anesthesia emergency]. [isoket对拔管及麻醉急诊血流动力学的影响]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-08-01

Yun-jiao Wang, Zhi-gang Cheng, Qu-lian Guo

引用次数: 0

[Microsurgical treatment of trigeminel neurinomas with middle fossa extradural approach]. [中窝硬膜外入路显微外科治疗三叉神经鞘瘤]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-06-01

Zhi-xiong Liu, Xian-rui Yuan, Jing-ping Liu

引用次数: 0

[Gene expression profile changes in human multiple myeloma]. [人类多发性骨髓瘤基因表达谱的变化]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-06-01

Yi-wu Shi, Wei-xin Hu, Li-jun Tang

引用次数: 0

[Clinical value comparison between chemiluminescent immunoassay and immunoradiometric assay in detecting TSH in serum]. 化学发光免疫法与免疫放射法检测血清TSH的临床价值比较。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-06-01

Jian-ying Yu, Ying Lin

引用次数: 0

[Adsorbent effect of activated carbon on small molecular uremic toxin and its influence factors]. [活性炭对小分子尿毒症毒素的吸附作用及其影响因素]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-06-01

Bo Yang, Yun-sheng Jiang, Jun Li

{"title":"[Adsorbent effect of activated carbon on small molecular uremic toxin and its influence factors].","authors":"Bo Yang, Yun-sheng Jiang, Jun Li","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To analyze the adsorbent effect of activated carbon on uremic toxin and its influence factors.Methods: Uremic toxins (urea, creatinine and uric acid) were dissolved in the distilled water to obtain uremic toxic solution. Activated carbon was added to the solution, and the concentrations of uremic toxins were measured at different time spots. To determine the influence factors, some possible related materials, such as bile, amino acid, Ringer's, solution of glucose, HCl or NaOH respectively were added simultaneously.Results: The concentrations of toxins in uremic toxic solution decreased 5 min after adding the activated carbon. The concentration of urea was the lowest at 30 min, but it increased after 50 min; while the concentrations of creatinine and uric acid reached the lowest level from 10 to 30 min after adding the activated carbon, and maintained at the same level after that. The bile, amino acid, electrolyte, glucose and pH value did not influence the adsorption of uric acid significantly, but they influenced the adsorption of urea and creatinine. Bile and amino acid influenced the concentration of urea remarkably, following glucose, NaOH and HCl. The effect of pH 2.0 solution on the creatinine concentration was the most significant, following glucose.Conclusion: Activated carbon has adsorptive effect on uremic toxins, but its adsorptive effect decreases as time goes on. Bile, glucose, amino acid, NaOH and HCl can affect the adsorptive effect of activated carbon on uremic toxins to some extent.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 3","pages":"285-7"},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24106250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Purification of human MMP-1 fusion protein and clinical detection of MMP-1 by its polyclonal antibody]. [人MMP-1融合蛋白的纯化及其多克隆抗体的临床检测]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-06-01

Jue Hou, Guo-ling Hu, Shuang-hu Liu

{"title":"[Purification of human MMP-1 fusion protein and clinical detection of MMP-1 by its polyclonal antibody].","authors":"Jue Hou, Guo-ling Hu, Shuang-hu Liu","doi":"","DOIUrl":"","url":null,"abstract":"Objectives: To obtain the fusion protein of MMP-1 and the rabbit-anti-human matrix metalloproteinase-1(MMP-1) polyclonal antibody, and to detect the serum MMP-1 in patients by this polyclonal antibody.Methods: The fusion protein was purified by affinity chromatography. Two rabbits were immunized with the purified fusion protein, and the immune sera of rabbits were collected. Antibodies (IgG) obtained from the immune sera were purified by ammonium sulfate fractionation, and the indirect sandwich enzyme immunoassay was used to detect the serum MMP-1 in 24 samples of patients with chronic liver disease or cirrhosis and in 12 normal sera as controls.Results: The purified MMP-1 fusion protein was successfully obtained; the purified specific polyclonal antibodies of rabbit-anti-human MMP-1 were also obtained from the immune sera of the rabbits, and could respond to human MMP-1. Compared with the normal controls, the OD value of serum MMP-1 significantly increased in patients with chronic hepatitis or liver cirrhosis (P < 0.01).Conclusion: The rabbit-anti-human MMP-1 polyclonal antibody may be used in the diagnosis of initial stage of hepatic fibrosis.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 3","pages":"240-2"},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24106323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Intravenous metastasis of primary hepatocarcinoma]. 原发性肝癌的静脉转移。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-06-01

Zong-yong Cheng, Xiao-wei Liu, Fang-gen Lu

引用次数: 0

[Construction and sequencing of recombinant plasmid pcDNA3/GRA1 from Toxoplasma gondii]. [刚地弓形虫重组质粒pcDNA3/GRA1的构建与测序]。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-06-01

Li-ting Cai, Heng-ping Shu, Li-ping Jiang

引用次数: 0

[Expression of vascular endothelial growth factor in the peritoneal fluid and eutopic endometrium of patients with endometriosis]. [血管内皮生长因子在子宫内膜异位症患者腹膜液和异位子宫内膜中的表达]

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-06-01

Xiao-ling Fang, Xiao-meng Xia, Qiu-hua Lin

{"title":"[Expression of vascular endothelial growth factor in the peritoneal fluid and eutopic endometrium of patients with endometriosis].","authors":"Xiao-ling Fang, Xiao-meng Xia, Qiu-hua Lin","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the significance of vascular endothelial growth factor (VEGF) on the etiology of endometriosis.Methods: The level of VEGF in the peritoneal fluid from women with endometriosis (n = 19) and non-endometriosis (n = 21) was studied by ELISA. The expression of VEGF of eutopic endometrium collected from women with endometriosis (n = 15) and non-endometriosis (n = 12) was determined by relative quantity RT-PCR.Results: The level of VEGF in the peritoneal fluid in the endometriosis was higher than that of the controls (P < 0.05). Cyclic variation in VEGF concentration was seen in the peritoneal fluid from patients with endometriosis, and the VEGF concentration in the proliferative phase was significantly higher than that in the secretory phase (P < 0.05). No cyclic variation in VEGF was seen in the control group (P > 0.05). The expression of VEGF in the eutopic endometrium with endometriosis was significantly higher than that of the controls (P < 0.001). There was no significant difference between the proliferative and secretory phase in endometriosis (P > 0.05).Conclusion: The increase of VEGF in the peritoneal fluid and eutopic endometrium with endometriosis may be important to the occurrence and development of endometriosis.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 3","pages":"288-90"},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24106251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Morphologic and hydrodynamic characteristics of bovine jugular venous conduit with valves]. 牛颈静脉带瓣膜导管的形态学和流体动力学特征。

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-06-01

Zhong-shi Wu, Jing-chao Zhang, Duan Cheng

{"title":"[Morphologic and hydrodynamic characteristics of bovine jugular venous conduit with valves].","authors":"Zhong-shi Wu, Jing-chao Zhang, Duan Cheng","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To determine the morphologic and hydrodynamic characteristics of bovine jugular venous conduit with valves.Methods: Fresh bovine jugular veins with a retained native valve procured from a slaughterhouse were used as raw material to fabricate the valved conduits. The hydrodynamic performance of fresh and glutaraldehyde fixed bovine jugular vein conduits with valves was investigated in an experimental flow equipment that provided a constant pressure. The perfusion flow quantity through the conduits was measured under the antegrade (from the distal end to the proximal end) and retograde (from the proximal to the distal end) perfusion. The parameters of the valves in these bovine jugular venous conduits were also measured.Results: There were 2-3 group valves in the bovine jugular venous conduit, with 3 or 2 valves in each group. The degrees of the leaflet development in different valves were different in the same conduit. The location of well-developed valves was interspersed. The anti-reflow ability of valves was positively correlated to the ratio between the leaflet height and valve sinus height (r = 0.709, P < 0.001). The percentage of retrograde and antegrade perfusion flow quantity after the glutaraldehyde treatment significantly increased than that before the glutaraldehyde treatment (P < 0.01).Conclusion: Part of the bovine jugular venous conduit has well-developed valves with strong ability against reflowing. Routine glutaraldehyde treatment obviously decreases the anti-reflow function of the conduits.","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 3","pages":"298-300"},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24105574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0