Hacettepe Journal of Biology and Chemistry最新文献_第3页

A practical and single-use ITO-PET based immunosensing platform for detection of Tumor Necrosis Factor-alpha biomarker 一个实用的、一次性的、基于ITO-PET的检测肿瘤坏死因子- α生物标志物的免疫传感平台

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-01-20 DOI: 10.15671/hjbc.1215813

Burcu Özcan

{"title":"A practical and single-use ITO-PET based immunosensing platform for detection of Tumor Necrosis Factor-alpha biomarker","authors":"Burcu Özcan","doi":"10.15671/hjbc.1215813","DOIUrl":"https://doi.org/10.15671/hjbc.1215813","url":null,"abstract":"This investigation displays a novel, practical indium tin oxide- polyethylene terephthalate (ITO-PET) based electrochemical biosensor for the Tumor Necrosis Factor-alpha (TNFα) biomarker determination. The ITO-PET electrode is a very advantageous preferred semiconductive electrode material. It has a lot of great features such as easy to prepare, cheapness, flexibility, stability. It also allows determining an analyte at very low concentrations and provides a great wide concentration range for analyte analysis. Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV) were used for the evaluation of biosensors, including the immobilization procedure, the investigation of the optimum conditions, and the characterization of biosensors. The immunosensor's electrode surface morphology during the immobilization process was observed using a Scanning Electron Microscope (SEM). In addition, Impedance measurement at a single frequency was used to characterize anti-TNFα and TNFα interactions (SFI). The clinical effectiveness of the developed biosensor was investigated by testing it with real human serum samples. Moreover, the fabricated immunosensor presents long shelf life, analysis of the antigen concentrations at picogram level (0.02 pg mL-1 -2.56 pg mL-1), reproducibility, reusability (11 times) and high sensitivity.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82606944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

INVESTIGATION OF ELECTROCHEMICAL PROPERTIES OF UA AT AL2O3NP/CPE AND DEVELOPMENT OF VOLTAMMETRIC METHOD FOR ITS DETERMINATION IN SERUM al2o3np / cpe中ua电化学性质的研究及血清中ua伏安法测定方法的建立

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-01-12 DOI: 10.15671/hjbc.1225829

Rüveyda Üver, F. Öztürk

引用次数: 0

Programmable living nanorobots 可编程的活纳米机器人

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-01-11 DOI: 10.15671/hjbc.1208034

Ammar Zi̇dan, Süleyman Aşır

引用次数: 1

CHITOSAN FILMS PREPARED WITH LOW NANOMETAL CONTENT FOR DEVELOPING PROTECTIVE DENIM FABRICS 低纳米金属含量壳聚糖薄膜用于牛仔防护织物的研制

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-01-02 DOI: 10.15671/hjbc.1127131

S. Erdoğan, Nilgün Becenen

引用次数: 0

Effects of Silica/Clay Nanoparticles on Microstructural and Mechanical Properties of Epoxy Based Adhesives 二氧化硅/粘土纳米颗粒对环氧基胶粘剂微观结构和力学性能的影响

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-01-01 DOI: 10.15671/hjbc.1192751

Şehram Di̇zeci̇, A. Kandemir

引用次数: 1

Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field 强效生物表面活性剂产生菌枯草芽孢杆菌CC9对石油的强化脱除作用

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-12-14 DOI: 10.15671/hjbc.1187546

S. Bilen Ozyurek, Kaan Soyuer

{"title":"Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field","authors":"S. Bilen Ozyurek, Kaan Soyuer","doi":"10.15671/hjbc.1187546","DOIUrl":"https://doi.org/10.15671/hjbc.1187546","url":null,"abstract":"This study aimed to enhance the removal of petroleum with potent biosurfactant producing bacterial strains (Bacillius subtilis CC9, Enterobacter asburiae WMB1, Klebsiella oxytoca Rizhao 536-1, and Acinetobacter pittii PgBE252) isolated from an oil field. The biosurfactant production was determined by different screening methods such as hemolytic activity, drop-collapse, oil spreading, BATH, emulsification index and surface tension. While the presence of biosurfactant was detected with individual bacterial strain, B. subtilis CC9 strain was found to be quite effective on petroleum degradation (92%) with a high yield of biosurfactant production (0.95 ±0.05 g L-1). The degradation rate constant and half-life period of B. subtilis CC9 strain were calculated as K = 2.533 day−1, t1/2 = 0.273. It has been clearly shown with GC/MS analysis that B. subtilis CC9 strain was effective on the removal of long chain n-alkanes (C31 - C35) as well as short and medium chains. The FT-IR analysis also approved the presence of aliphatic and peptide moieties of biosurfactant extracted from B. subtilis CC9 strain. It is remarkably pointed out with this study that high yield of biosurfactant producing B. subtilis CC9 strain can be used in advanced petroleum bioremediation studies as an alternative to chemical surfactants.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83763709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EVALUATION AND COMPARISON OF THE ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF SOME AMINO ACID METHYL ESTERS 几种氨基酸甲酯抗菌和细胞毒活性的评价与比较

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-11-01 DOI: 10.15671/hjbc.1183477

T. Karaca, Huseyin Balci, Arzu Aysan

引用次数: 1

Tuzaklamalı İyon Hareketliliği-Kütle Spektrometrisi Tekniği Kullanılarak İnsülinin Bakır ve Alkali Metal Komplekslerinin Konformasyonel Farklılıklarının Analizi

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-10-14 DOI: 10.15671/hjbc.1150033

Mehmet Atakay

{"title":"Tuzaklamalı İyon Hareketliliği-Kütle Spektrometrisi Tekniği Kullanılarak İnsülinin Bakır ve Alkali Metal Komplekslerinin Konformasyonel Farklılıklarının Analizi","authors":"Mehmet Atakay","doi":"10.15671/hjbc.1150033","DOIUrl":"https://doi.org/10.15671/hjbc.1150033","url":null,"abstract":"Moleküler tanıma, protein katlanması ve supramoleküler yapıların oluşumu gibi moleküler düzeyde meydana gelen biyolojik süreçler kovalent olmayan etkileşimlere dayanır. Metal atomları ve proteinler arasındaki etkileşimler de birçok hücresel süreçte yer alan mekanizmaların temelini oluşturan kovalent olmayan etkileşimlere dayanmaktadır. Enzimlerin aktiviteleri, bu tür protein gruplarının kofaktörler, substratlar, metal iyonları ve diğer proteinlerle olan etkileşimlerine büyük ölçüde bağlıdır. Protein-metal komplekslerinin bileşimleri ve bağlanma stokiyometrileri, yüksek doğrulukta kütle spektrometrik (MS) analiz ile belirlenebilir. Protein-metal komplekslerinin konformasyonel özellikleri ise iyon hareketliliği spektrometrisi (IMS) özelliğine sahip bir kütle spektrometresi kullanılarak ek olarak incelenebilmektedir. Bu çalışmada tuzaklamalı iyon hareketliliği spektrometrisi - uçuş zamanlı (TIMS-TOF) kütle spektrometresi kullanılarak bakır ve alkali metallerle komplekslerinin oluşumu sırasında insülin proteininin konformasyonel değişimlerindeki farklılıkların izlenmesine odaklanılmaktadır. İnsülin-bakır komplekslerinin oluşmasıyla insülin yapısının gaz fazında daha kompakt hale geldiği TIMS-TOF-MS analizleri ile belirlenmiştir. Ancak aynı analiz koşullarında H, Na ve K atomlarının eklenmesiyle insülin yapısında herhangi bir değişiklik gözlenmemiştir.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82209730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benchmarking polymeric cryogels for immobilized metal affinity chromatography 固定化金属亲和层析用聚合物低温对标

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-10-11 DOI: 10.15671/hjbc.1166975

Yeşeren Saylan

{"title":"Benchmarking polymeric cryogels for immobilized metal affinity chromatography","authors":"Yeşeren Saylan","doi":"10.15671/hjbc.1166975","DOIUrl":"https://doi.org/10.15671/hjbc.1166975","url":null,"abstract":"Cryogels are polymers prepared in frozen environment and seen as new separation matrices with their applications in many bioseparation methods. They have significant benefits including supermacroporosity, short diffusion path, low pressure, and low resistance to both adsorption and elution. Macro and connected pores give cryogels a unique spongy structure. Immobilized metal affinity chromatography (IMAC) is a generally employed analytical separation method for the purification of biomolecules. Several transition ions create stable complexes with electron-rich compounds. IMAC sorbent is obtained by complexing first-order transition metal ions over chelating agents. Lysozyme is an enzyme found in various vertebrate cells and secretions. Common applications include its use as a cell disrupting agent, as an anti-bacterial agent, as a food additive, and as a medicine for the treatment of infections and ulcers. In this study, cryogel-based polymeric material prepared by free-radical polymerization method with hydroxyethyl methacrylate/glycidyl methacrylate monomer pair were covalently interacted with iminodiacetic acid metal chelating agent, and regions showing affinity for lysozyme enzyme were formed by binding with Ni(II) ions. The cryogel was first characterized by Fourier transform infrared spectrophotometer, scanning electron microscopy, thermal gravimetric analysis, X-ray photoelectron spectroscopy and swelling test. Then, the effects of pH, lysozyme concentration, temperature, ionic strength and flow rate on lysozyme adsorption capacity were explored and optimum conditions were found.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"233 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76109250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Docking Mediated Virtual Drug Screening for GABAA Receptor: Promising Digoxin Derivatives 分子对接介导的GABAA受体虚拟药物筛选:有前途的地高辛衍生物

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-10-08 DOI: 10.15671/hjbc.1139995

H. S. Portakal

{"title":"Molecular Docking Mediated Virtual Drug Screening for GABAA Receptor: Promising Digoxin Derivatives","authors":"H. S. Portakal","doi":"10.15671/hjbc.1139995","DOIUrl":"https://doi.org/10.15671/hjbc.1139995","url":null,"abstract":"In the central nervous system (CNS) of mammalian species, ℽ-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter due to it regulates neuronal development through leading neural differentiation, proliferation, migration, etc. GABAA receptor is the major GABA receptor since it has the highest expression level among the other GABA receptors within CNS. Many pieces of evidence prove that the defects in the GABAergic pathway might give rise to serious diseases such as schizophrenia, epilepsy, anxiety, depression, insomnia, etc. In this study drug library with a totally of 8170 ligands consists of three distinct datasets which are FDA-approved Drugs, Drugs Approved by World but not FDA, and Non-human Metabolites have been screened for the allosteric site of the GABAA receptor with PyRx Virtual Screening Tool and ligand-receptor interactions have been analyzed with Biovia Discovery Studio software. Results reveal that Digoxin and its two distinct derivatives (DD1 and DD2), as well as Conivaptan, are promising in the treatment of GABAergic pathway-based disorders. The findings of this report should be verified with further molecular dynamics (MD) simulations and the ligands should be tested by both in vitro and in vivo studies.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88496062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0