Hacettepe Journal of Biology and Chemistry最新文献_第4页

Structural Health Monitoring by Using Fiber Optic Sensors in Composite Materials 基于复合材料光纤传感器的结构健康监测

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-07-27 DOI: 10.15671/hjbc.1066965

M. O. Kaman, Muhammed Ali Yilmaz, Gökhan Ayti̇mur, Enes Altin, Serkan Erdem

引用次数: 0

High Pressure Resistant Aramid Fiber Reinforced Polymer Matrix Composite Pipe Design 耐高压芳纶纤维增强聚合物基复合材料管道设计

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-07-27 DOI: 10.15671/hjbc.1024810

Ali Ari, A. Bayram

{"title":"High Pressure Resistant Aramid Fiber Reinforced Polymer Matrix Composite Pipe Design","authors":"Ali Ari, A. Bayram","doi":"10.15671/hjbc.1024810","DOIUrl":"https://doi.org/10.15671/hjbc.1024810","url":null,"abstract":"In this study, a total of 6 different composites were obtained by adding 5%, 10% and 15% chopped aramid fiber reinforcement in 6 mm dimensions to each of the polypropylene and polyethylene matrix elements under the same production method and same conditions. The reinforcement and matrix materials were mixed using the extrusion method and then formed into plates by the press molding technique. Tensile and charpy tests of these composites were performed and their mechanical properties were examined. S/N ratios were calculated for the mechanical properties of the composites and the effect of matrix, fiber and additive ratios on mechanical properties was determined using analysis of variance (ANOVA). According to the Signal/Noise (S/N) ratios and ANOVA results, it was observed that the composites had different effects on the mechanical properties. Pipes are designed considering the mechanical properties of composite materials with 15% aramid fiber added to each matrix element. The composite pipe design to be pressure tested was designed in Solidworks program with a length of 500 mm according to ISO 1167 standards. Pipe dimensions with an outer diameter of 125mm, which are used as a standard in natural gas infrastructure works, are taken as reference.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89358763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of Antimicrobial Activities of Various Herbal Oils Against Helicobacter pylori And Their Cytotoxic Effects On HUVEC Cell Line 不同草药精油对幽门螺杆菌的抑菌活性及其对HUVEC细胞的细胞毒作用

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-06-12 DOI: 10.15671/hjbc.1114101

T. Karaca, B. Aru, K. Çağlar

{"title":"Evaluation of Antimicrobial Activities of Various Herbal Oils Against Helicobacter pylori And Their Cytotoxic Effects On HUVEC Cell Line","authors":"T. Karaca, B. Aru, K. Çağlar","doi":"10.15671/hjbc.1114101","DOIUrl":"https://doi.org/10.15671/hjbc.1114101","url":null,"abstract":"Helicobacter pylori (H. pylori) enfeksiyonu en önemli kronik bakteriyel enfeksiyon olarak kabul edilmektedir. Son yıllarda tedavide kullanılan antibiyotiklere karşı bakterinin direnç geliştirdiği rapor edilmiştir. Tedavide başarı oranının yükselmesi ve rekürrensin azalması için, toksik olmayan biyouyumlu bitkisel kaynakların araştırılarak sistemik antibiyotik tedavisine ek olarak kullanılması ve eradikasyonun sağlanması son derece önemlidir. Bitkilerden elde edilen yağlar uzun yıllardan beri çeşitli amaçlara yönelik, özellikle ticari ve bilimsel alanlarda kullanılmaktadır. Bu nedenle çalışmamızda; genellikle mide ve gastrointestinal sistem rahatsızlıklarına iyi geldiği bilinen ve literatürde H.pyloriüzerinde yapılmış yeterli çalışması bulunmayan çeşitli bitkisel yağlar (Eucalyptus globulus, Juniperus communis, Rosmarinus officinalis, Thymus vulgaris) seçilmiş ve H. pylori'ye karşı antimikrobiyal etkinlikleri ile Human Umbilical Vein Endothelial Cells (HUVEC) hücre soyu üzerindeki sitotoksik etkilerinin araştırılması amaçlanmıştır. Bitkisel yağların antimikrobiyal etkinlikleri mikrodilüsyon yöntemi ile (MIC, MBC), sitotoksik etkinlikleri ise MTT ve LDH yöntemleri ile incelenmiştir. H.pylori’ye karşı antimikrobiyal etkisini araştırdığımız, Eucalyptus globulus (MIC: 2,81 %v/v, MBC: 5,62 %v/v), Juniperus communis (MIC: 0,35 %v/v, MBC: 0,70 %v/v), Rosmarinus officinalis (MIC: 2,81 %v/v, MBC: 5,62 %v/v) ve Thymus vulgaris (MIC: 0,70 %v/v, MBC: 1,40 %v/v) 'in etkili olduğu gözlemlenmiştir. Bunun yanında HUVEC hücre soyu üzerinde Thymus vulgaris bitkisel yağının en yüksek, Eucalyptus globulus bitkisel yağının ise en düşük sitotoksik etkiye sahip olduğu tespit edilmiştir. \u0000Anahtar Kelimeler: Helicobacter pylori, Bitkisel yağ, Antibakteriyel aktivite, Sitotoksisite","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76011987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiproliferative properties and evaluation of antioxidant of different cornelian cherry genotypes and analysis of phenolic and sugar compounds by HPLC 不同山茱萸基因型的抗增殖特性、抗氧化性评价及酚类和糖类化合物的HPLC分析

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-06-07 DOI: 10.15671/hjbc.1065317

Y. Uğur, Emine Şalva, F. Karaat, S. Erdoğan

{"title":"Antiproliferative properties and evaluation of antioxidant of different cornelian cherry genotypes and analysis of phenolic and sugar compounds by HPLC","authors":"Y. Uğur, Emine Şalva, F. Karaat, S. Erdoğan","doi":"10.15671/hjbc.1065317","DOIUrl":"https://doi.org/10.15671/hjbc.1065317","url":null,"abstract":"Bu çalışmanın amacı, kızılcık meyvelerinin kimyasal bileşimi (C vitamini, bireysel fenolik ve şeker bileşikleri) ve antioksidan kapasitesinin yanında, meyve ekstrelerinin sağlıklı (L-929) ve akciğer kanseri (A-549) hücreleri üzerindeki sitotoksik etkisini araştırmaktır. Türkiye'de aynı koşullarda yetiştirilen on üç kızılcık (Cornus mas L.) genotipi meyve örnekleri ASE tekniği ile optimum koşullarda ekstrakte edildikten sonra kimyasal bileşim, HPLC-DAD-RID ile analiz edildi. Antioksidan kapasitesi, toplam fenolik ve toplam antosiyanin içeriği spektrofotometrik yöntemler kullanılarak belirlendi. Sitotoksik etkiler, 48 saat boyunca L-929 ve A-549 hücre hatlarında MTT testi ile değerlendirildi. Meyve ekstraktlarının L-929 sağlıklı fare fibroblast hücreleri üzerinde toksik etkisi gözlenmezken, A-549 akciğer kanseri hücrelerinde hücre proliferasyonunu (yaklaşık %50) azalttığı belirlendi. A-549 hücreleri üzerindeki antioksidan kapasite ve sitotoksik etkiler için öne çıkan genotipler sırasıyla 44-03, 44-20, 44-21, 77-09 ve 44-21, 44-16, 77-05 olduğu belirlendi. Elde edilen sonuçlar, genotipler (p ≤ 0.05) arasında önemli farklılıklar olduğunu ve kızılcık meyvelerinin önemli bir antioksidan kapasiteye ve antiproliferatif etki potansiyeline sahip olduğunu ortaya koymuştur.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82014750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic Studies on the Male Genital Organs of some Blister Beetles (Coleoptera: Meloidae) of Ankara Province (Turkey) 土耳其安卡拉省部分水疱甲虫(鞘翅目:花虱科)雄性生殖器官的系统研究

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-06-07 DOI: 10.15671/hjbc.870339

M. Demir, M. Kabalak

引用次数: 0

Automated Cell Viability Analysis in Tissue Scaffolds 组织支架中的自动细胞活力分析

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-06-07 DOI: 10.15671/hjbc.868396

Tansel Uyar, A. Erdamar, M. Gümüşderelioğlu, M. Aksahin, Gülseren Irmak, Osman Erogul

{"title":"Automated Cell Viability Analysis in Tissue Scaffolds","authors":"Tansel Uyar, A. Erdamar, M. Gümüşderelioğlu, M. Aksahin, Gülseren Irmak, Osman Erogul","doi":"10.15671/hjbc.868396","DOIUrl":"https://doi.org/10.15671/hjbc.868396","url":null,"abstract":"Image processing techniques are frequently used for extracting quantitative information (cell area, cell size, cell counting, etc.) from different types of microscopic images. Image analysis of cell biology and tissue engineering is time consuming and requires personal expertise. In addition, evaluation of the results may be subjective. Therefore, computer-based learning applications have been rapidly developed in recent years. In this study, Confocal Laser Scanning Microscope (CLSM) images of the viable pre-osteoblastic mouse MC3T3-E1 cells in 3D bioprinted tissue scaffolds, captured from a bone tissue regeneration study, were analyzed by using image processing techniques. The goal of this study is to develop a reliable and fast algorithm for semi-automatic analysis of CLSM images. Percentages of live and dead cell areas in the scaffolds were determined with image correlation, and then, total cell viabilities were calculated. The other goal of this study is to determine the depth profile of viable cells in 3D tissue scaffold. Manual measurements of four different analysts were obtained. The measurement variations of analysts, also known as the coefficient of variation, were determined from 13.18% to 98.34% for live cell images and from 9.75% to 126.02% for dead cell images. To overcome this subjectivity, a semi-automatic algorithm was developed. Consequently, cross-sectional image sets of three different types of tissue scaffolds were analyzed. As a result, maximum cell viabilities were obtained at intervals of 63 µm and 90 µm from the scaffold surface.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74300724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRELIMINARY PHYTOCHEMICAL SCREENING, GC-MS, FTIR ANALYSIS OF ETHANOLIC EXTRACTS OF ROSMARINUS OFFICINALIS, CORIANDRUM SATIVUM L AND MENTHA SPICATA 迷迭香、芫荽和薄荷乙醇提取物的初步植物化学筛选、气相色谱-质谱分析

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-06-03 DOI: 10.15671/hjbc.1073300

J. Mohammed, Oluwasuyi Ayobami Oba, Nur Pasaoglulari Aydinlik

{"title":"PRELIMINARY PHYTOCHEMICAL SCREENING, GC-MS, FTIR ANALYSIS OF ETHANOLIC EXTRACTS OF ROSMARINUS OFFICINALIS, CORIANDRUM SATIVUM L AND MENTHA SPICATA","authors":"J. Mohammed, Oluwasuyi Ayobami Oba, Nur Pasaoglulari Aydinlik","doi":"10.15671/hjbc.1073300","DOIUrl":"https://doi.org/10.15671/hjbc.1073300","url":null,"abstract":"Phytochemical and some proximate composition analysis was carried out on three (3) selected edible medicinal plants leaves which were believed to be of medicinal value and have physiological effects as anti-inflammatory, antibacterial, anti-pyretic, antioxidant, laxative etc. The significant aim of the research is to identify the phyto-components as well as compounds presents in the ethanolic extract of Mentha spicata L (mints), Rosemarinus officinalis (Rosemary), and Coriandrum sativum (coriander) using two different analytical methods GC-MS along with their functional groups using FTIR. The GC-MS analysis reveals more than thirty (30) compounds identified as major constituents and mostly all the compounds identified was found to possess medicinal properties. The data analysis from FTIR spectrometry representing most of the strong absorptions bands which further indicates major functional groups such as aliphatic amines, alkanes, aromatic (primary and secondary) and carboxylic acids. While the preliminary phytochemical screening conducted indicates the presence of Flavonoids, tannins, triterpenoids, and saponins from the ethanolic extracts of the plant extracts. Therefore the findings indicate that all the selected plant samples are potential sources of medicinal activities and can be applied in the field of phyto-medicine considering their diverse ethno-pharmacological importance.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"SE-10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84636403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect Of Ball Milling Time on Microstructural Properties of Mg/MgO 球磨时间对Mg/MgO显微组织性能的影响

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-04-07 DOI: 10.15671/hjbc.1032620

Y. Yahsi, R. Ipek

引用次数: 0

Effects of Molecular Weight of Dextran on Dynamic Mechanical Properties in Functional Polymer Blend Systems 右旋糖酐分子量对功能聚合物共混体系动态力学性能的影响

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-04-07 DOI: 10.15671/hjbc.1076097

S. Kavlak

{"title":"Effects of Molecular Weight of Dextran on Dynamic Mechanical Properties in Functional Polymer Blend Systems","authors":"S. Kavlak","doi":"10.15671/hjbc.1076097","DOIUrl":"https://doi.org/10.15671/hjbc.1076097","url":null,"abstract":"Blending of natural/synthetic polymers is one of the most practical way to obtain a new material with desired properties such as thermal, mechanical and dynamic mechanical properties. Dynamic mechanical analysis (DMA) is a strong method to investigate the mechanical/viscoelastic properties, thermal transitions and compatibility in these polymer blend systems. In this study, biocompatible T10 and T40 dextran (DEX) and polymethacrylamide (PMAM) blend systems were prepared by solvent casting method. Variations of dynamic mechanical properties including storage modulus (SM), loss modulus (LM) and tan δ of the DEX/PMAM blends were investigated for all samples at a specific fixed frequency of dynamic mechanical loading in a certain temperature range. Thermal transitions and -relaxations were observed from results of DMA measurements. In general, a single glass transition temperature was observed in binary blend systems. It was found that temperature dependence of dynamic mechanical properties and curves exhibit typical behaviors and strongly depended on the molecular weight, intra- and intermolecular interactions due to the hydrogen bonding in these blend systems.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79035283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Green synthesis of silver nanoparticles using Salvia fruticosa Mill. extract and the effect of synthesis parameters on their formation, antioxidant, and electro-catalytic activity 利用鼠尾草磨绿色合成纳米银。萃取物及其合成参数对其形成、抗氧化及电催化活性的影响

Hacettepe Journal of Biology and Chemistry Pub Date : 2022-04-07 DOI: 10.15671/hjbc.1040656

Damla Erkakan, N. Diker, M. Önal, İffet İrem Çankaya

{"title":"Green synthesis of silver nanoparticles using Salvia fruticosa Mill. extract and the effect of synthesis parameters on their formation, antioxidant, and electro-catalytic activity","authors":"Damla Erkakan, N. Diker, M. Önal, İffet İrem Çankaya","doi":"10.15671/hjbc.1040656","DOIUrl":"https://doi.org/10.15671/hjbc.1040656","url":null,"abstract":"Among green synthesis methods, which are an eco-friendly, non-toxic, simple, and safe approach for the synthesis of silver nanoparticles (AgNPs), using plant extract is the most efficient method. Salvia fruticosa Mill. which was not used formerly was selected for this research. By changing the synthesis parameters (the amount of extract, extract concentration, and silver ion concentration in precursors), their effects on the formation and structure of nanoparticles were investigated by UV-visible spectroscopy, transmission electron microscopy and Fourier transform infrared spectroscopy techniques. The antioxidant activity of extracts and AgNPs was evaluated by performing DPPH assay. It is observed that the phytosynthesized nanoparticles also possess antioxidant potentials. Finally, AgNPs were used as modifiers for carbon paste electrode (CPE) and their effect on charge transfer resistance and the ascorbic acid signal was investigated by using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and square wave voltammetry (SWV). E1-1/CPE showed good electro-catalytic oxidation of ascorbic acid and can be utilized for the development of the new sensors. According to results, in the process of green synthesis of AgNPs, synthesis parameters are vital as they change not only the size and size distribution of the AgNPs but also their antioxidant activity and electrochemical properties.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87073422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0