Hacettepe Journal of Biology and Chemistry最新文献

COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS 生物聚合物包覆电纺丝支架的神经胶质保护作用比较

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-11-07 DOI: 10.15671/hjbc.1245678

Ece BATTALOĞLU, Zehra Gül MORÇİMEN, Aylin ŞENDEMİR

引用次数: 0

Assessment of Staphylococcal toxins acting as superantigens in different nasal specimens in the etiology of chronic rhinosinusitis 慢性鼻窦炎病因学中不同鼻标本中葡萄球菌毒素作为超级抗原的评估

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-11-07 DOI: 10.15671/hjbc.1302331

Gülden VURAL, Alper CEYLAN, M. Nedim SULTAN

{"title":"Assessment of Staphylococcal toxins acting as superantigens in different nasal specimens in the etiology of chronic rhinosinusitis","authors":"Gülden VURAL, Alper CEYLAN, M. Nedim SULTAN","doi":"10.15671/hjbc.1302331","DOIUrl":"https://doi.org/10.15671/hjbc.1302331","url":null,"abstract":"The mechanism of development of chronic rhinosinusitis(CRS) is not fully known. However, bacteria are thought to play an important role in this clinic. It has been suggested that toxins with superantigen(SAgs) properties produced by one of these bacteria, Staphylococcus aureus(S.aureus), play a role in the development of inflammation associated with sinusitis. In this study, S.aureus was isolated by taking endoscopic sinus biopsy samples and nasal swab samples from patients with CRS and the control group. It was aimed to examine the frequency of S.aureus presence in the samples taken, the presence of toxin genes showing superantigen quality in these isolated bacteria, and to evaluate the roles of these parameters in the development of CRS. More S.aureus was isolated in the samples taken from patients with CRS than in the control group. The isolated S.aureus samples were analysed by real-time PCR method. The presence of enterotoxin A, B, C and D genes in the S.aureus samples isolated from the patient group were found at the rates of 54%, 32%, 16% and 16%, respectively, while these rates were 46%, 24%, 14% and 14% in the control group. The Toxic Shock Syndrome Toxin-1(TSST-1) gene was detected in 20% of the samples isolated from the patient and 46% in the control group bacteria. The fact that S.aureus was isolated in 20% of the patients shows that this bacterium is not necessary for CRS. The frequency of superantigen toxin genes in S.aureus isolates shows that these toxins are not necessary for the development of the disease.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"314 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135474790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE SHORT-TERM EFFECTS OF WHEAT STRAW CELLULOSE ON SOIL CARBON MINERALIZATION 麦秸纤维素对土壤碳矿化的短期影响

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-11-07 DOI: 10.15671/hjbc.1270451

Bahar MERYEMOĞLU, Nacide KIZILDAĞ ÖZDAL

{"title":"THE SHORT-TERM EFFECTS OF WHEAT STRAW CELLULOSE ON SOIL CARBON MINERALIZATION","authors":"Bahar MERYEMOĞLU, Nacide KIZILDAĞ ÖZDAL","doi":"10.15671/hjbc.1270451","DOIUrl":"https://doi.org/10.15671/hjbc.1270451","url":null,"abstract":"Cellulosic wastes constitute the majority of agricultural fields. The purpose of this study was to utilize these cellulosic wastes such as wheat straw and wheat straw cellulose on soil carbon mineralization in a sandy loam soils. Two different doses (100 and 1000 mg) of wheat straw cellulose were used to determine the carbon mineralization using CO2 respiration method. The cumulative carbon mineralization was found to be highest at the minumum doses of wheat straw cellulose with nitrogen (W-CL-N, 19.65 mg) and the lowest at the maximum doses of wheat straw (W-Straw, 14.32 mg). The results showed that the application of wheat straw cellulose at minumum doses resulted in higher carbon mineralization rate. The maximum carbon mineralization rate was observed in soil with minimum wheat straw cellulose and nitrogen source were added (1.41%). Whereas, the minimum carbon mineralization rate was determined in the soil mixed maximum wheat straw (1.03 %). The soil mixed maximum wheat straw was determined the lowest carbon mineralization rate due to its complex structure. The use of nitrogen source and organic matter with cellulose have positive effect on soil carbon mineralization. It might be said that these results describe an effective way for disposal of organic wastes.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"313 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135474792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

E. coli O157:H7 Detection Using Surface Plasmon Resonance Based Biosensor 基于表面等离子体共振的生物传感器检测大肠杆菌O157:H7

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-11-07 DOI: 10.15671/hjbc.1271685

Esma ESER, Okan Öner EKİZ, H. İbrahim EKİZ

{"title":"E. coli O157:H7 Detection Using Surface Plasmon Resonance Based Biosensor","authors":"Esma ESER, Okan Öner EKİZ, H. İbrahim EKİZ","doi":"10.15671/hjbc.1271685","DOIUrl":"https://doi.org/10.15671/hjbc.1271685","url":null,"abstract":"The detection of foodborne pathogenic bacteria remains a significant challenge, and the need for fast and sensitive detection methods is becoming increasingly important. Escherichia coli is a prevalent bacteria associated with foodborne illness, and this study aimed to evaluate the ability of a surface plasmon resonance (SPR) based biosensor to detect E. coli O157:H7 at low levels in pure culture and artificially contaminated bay leaves (Laurus nobilis) using different injection methods. To develop a biological sensing surface, the sensor surface was functionalized with 3-aminopropyltriethoxysilane (APTES), and polyclonal antibodies were immobilized on the surface for bacteria detection. Bacterial attachment to the antibodies resulted in a change in resonance angle. The biosensor was able to discriminate between cellular concentrations of 103 to 107 CFU/mL and showed potential in detecting different pathogens in various food samples. Before the SPR detection, the sample preparation step was optimized to ensure complex food matrices were suitable for SPR analysis. The results suggest that the SPR based biosensor is a promising tool for the rapid detection of foodborne pathogens in complex food matrices.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"313 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135474791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Content Analysis of Locally Marketed Energy Drinks: Turkish Market 土耳其市场本地销售的能量饮料含量分析

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-11-07 DOI: 10.15671/hjbc.1279376

Zafer PEKER, Dursun Ali KÖSE

{"title":"Content Analysis of Locally Marketed Energy Drinks: Turkish Market","authors":"Zafer PEKER, Dursun Ali KÖSE","doi":"10.15671/hjbc.1279376","DOIUrl":"https://doi.org/10.15671/hjbc.1279376","url":null,"abstract":"In the Turkish Food Codex Communique on Energy Drinks, energy drinks are defined as \"flavored non-alcoholic beverage containing caffeine, containing taurine, glucuronolactone, inositol, carbohydrates, amino acids, vitamins, minerals, and other food and components\". The amount of caffeine in the composition of the energy drink cannot be more than 150 mg/L, inositol 100 mg/L, glucuronolactone 20 mg/L, and taurine 800 mg/L. It has been reported that the consumption of beverages containing caffeine, taurine, and glucuronolactone in healthy young adults increases arterial blood pressure and platelet aggregation and decreases endothelial function. For healthy adults, consuming more than 400 mg of caffeine daily and consuming more than 500 ml of energy drinks in energy drinks, as well as consuming energy drinks with or together with alcohol, is risky and not recommended. Energy drinks are risky for children, people under the age of 18, the elderly, diabetics, those with high blood pressure, pregnant and lactating women, those with metabolic diseases, kidney failure, and people who are sensitive to caffeine. The use of energy drinks has been increasing rapidly in the world and in our country in recent years. Usually, energy drinks are used by young people, athletes, and people who live actively. Energy drinks are also preferred as a pleasure or to increase mental, physical, and cognitive performance. The aim of our study is to analyze the components of the content and the effectiveness of the components by making a qualitative analysis with the GC-MS (Gas Chromatography-Mass Analysis) device of many energy drinks used in the market. In addition, it is the evaluation of the energy drinks available in the market by examining the effects of the determined substances and their amounts on human health.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135431843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capsaicin Shows Species and Strain-specific Activity: Investigation of the Antibacterial Effects on the Oral Pathogen Streptococcus mutans and the Oral Probiotics Streptococcus salivarius M18 and K12 辣椒素具有物种和菌株特异性活性：研究辣椒素对口腔病原体变异链球菌和口腔益生菌唾液链球菌 M18 和 K12 的抗菌作用

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-10-25 DOI: 10.15671/hjbc.1337284

Kader Doğan, Sinem Tunçer

{"title":"Capsaicin Shows Species and Strain-specific Activity: Investigation of the Antibacterial Effects on the Oral Pathogen Streptococcus mutans and the Oral Probiotics Streptococcus salivarius M18 and K12","authors":"Kader Doğan, Sinem Tunçer","doi":"10.15671/hjbc.1337284","DOIUrl":"https://doi.org/10.15671/hjbc.1337284","url":null,"abstract":"Extensive research has investigated capsaicin (CAP), the primary bioactive compound in chili peppers, to explore its diverse pharmacological and physiological properties. Recently, the focus has shifted to understanding the potential effects of CAP on gut microbiota due to the strong link between gut bacterial profile and diet. However, there has been no research on the effects of CAP on oral microbiota. Therefore, our study aimed to explore the antibacterial effects of CAP on two oral probiotics, Streptococcus salivarius M18, and S. salivarius K12, along with the oral pathogen S. mutans. Previously, the anti-cancer activity of CAP had been demonstrated, and in accordance with these findings, here, we show its growth inhibitory activity on colorectal cancer cell lines. However, this study is the first to examine the impact of CAP on specific oral microorganisms while considering the oral consumption of CAP and the interconnectedness of the oral and gut microbiomes. The findings revealed that CAP exhibited antibacterial properties against the M18 strain at concentrations exceeding 100 µg/mL. Surprisingly, it did not show any growth-inhibitory effects on S. salivarius K12, even at a concentration of 2 mg/mL. Similarly, CAP did not inhibit the growth of S. mutans, a significant factor in dental caries. These results suggest that CAP's effects are species and strain-specific, indicating potential changes in the oral microbiota upon CAP consumption.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139314327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parental Care and Nest Site Properties in the European Roller, Coracias Garrulus in a Novel Breeding Site 一个新繁殖地的欧洲卷耳鹬的亲代抚育和巢地特性

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-10-25 DOI: 10.15671/hjbc.1342237

Necmiye ŞAHİN ARSLAN

{"title":"Parental Care and Nest Site Properties in the European Roller, Coracias Garrulus in a Novel Breeding Site","authors":"Necmiye ŞAHİN ARSLAN","doi":"10.15671/hjbc.1342237","DOIUrl":"https://doi.org/10.15671/hjbc.1342237","url":null,"abstract":"I investigated parental behavior and nest site occupancy of the European Roller, Coracias garrulus in a novel breeding site, retaining walls with numerous embedded 10 cm diameter pipes in 2020–2022 in northern Turkey, Corum. I also searched the literature to review parental behavior and length of parental care in egg and nestling stages in other members of the order Coraciiformes. European Rollers occupied 6 holes for 12 nesting attempts across three years. Nest height from the ground was 4.7 ± 0.3 m (n = 12). I video-recorded the nest holes in the early morning during the incubation and nestling periods for 22.9 and 25 hours in total, respectively. Males and females shared incubation and nestling provisioning tasks. Nest attentiveness was 97.9% ± 0.79. Average feeding rate of nestlings was 10.86 ± 2.15 trips h-1. Feeding rates increased from early (5.94 ± 1.09 h-1) to late (15.8 ± 2.04 h-1) nestling ages, significantly. Ultimately, the comparisons among species in the order showed that the vast majority of species were relatively similar in development times but that little data is available on parental behavior. The latter emphasizes the great need for more study of parental behavior in this order.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135218073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glaucium Cappadocicum: Screening on Antioxidant, Antimicrobial, Anticholinesterase Inhibition in vitro 体外抗氧化、抗菌、抗胆碱酯酶抑制作用的筛选

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-10-19 DOI: 10.15671/hjbc.1332418

Sevgi ALTIN, Ekrem KÖKSAL

引用次数: 0

Evaluation of Antifungal and Antiproliferative Effects of Two Different Organic Compounds with Cyclobutane Ring 两种含环丁烷的有机化合物的抗真菌和抗增殖作用的评价

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-07-05 DOI: 10.15671/hjbc.1311376

Mustafa Çiçek, T. Karaca, Ibrahim Yilmaz

{"title":"Evaluation of Antifungal and Antiproliferative Effects of Two Different Organic Compounds with Cyclobutane Ring","authors":"Mustafa Çiçek, T. Karaca, Ibrahim Yilmaz","doi":"10.15671/hjbc.1311376","DOIUrl":"https://doi.org/10.15671/hjbc.1311376","url":null,"abstract":"In the modern world, where there is a growing demand for new substances with anticancer and antifungal activities, Schiff bases and phthalimide derivatives, which exhibit a wide diversity of biological activity, have become the focus of new therapeutic research studies. Accordingly, this study examined the anti-proliferative effects of two distinct compounds synthesized by cyclobutane substitution on breast cancer and liver cancer cell lines, which are two major cancer types, were investigated with MTT method and their antifugal activities on C. albicans were evaluated with disk diffusion method. Minimum inhibitory concentrations of the compounds against C. albicans were also determined in the scope of the study. The results revealed that the synthesized Schiff base was more effective on the breast cancer cell line MCF7, whereas the phthalimide-derivative was more effective against the liver cancer cell line Mahlavu. Besides, according to the data related to the antifungal properties of the compounds, it can be inferred that both compounds are suitable for further investigation as potential building blocks for the creation of novel and efficient antifungal medications.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88542337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the Siderophore Production and Associated Heavy Metal Accumulation Potential of Brevibacillus laterosporus 301/İK3-2 侧芽孢短杆菌301/İK3-2铁载体产量及相关重金属积累潜力的研究

Hacettepe Journal of Biology and Chemistry Pub Date : 2023-06-08 DOI: 10.15671/hjbc.1256836

F. I. Özdemir, Bahadır Aydin, A. Tülek

{"title":"Investigation of the Siderophore Production and Associated Heavy Metal Accumulation Potential of Brevibacillus laterosporus 301/İK3-2","authors":"F. I. Özdemir, Bahadır Aydin, A. Tülek","doi":"10.15671/hjbc.1256836","DOIUrl":"https://doi.org/10.15671/hjbc.1256836","url":null,"abstract":"Siderophores are secondary metabolites released into the environment by various microorganisms, fungi, and plants to chelate iron from the surrounding environment. It is known that siderophores bind to other metals besides iron. Today, heavy metals, which are released as an undesirable result of industrial development, accumulate at high rates and pose a significant threat to biological living things. In this sense, remediation of heavy metal-contaminated sites is an urgent requirement. Siderophores are promising agents for the removal of heavy metals from natural habitats with the role of bioremediation. \u0000In this study, the effect of heavy metals on the growth and siderophore production of Brevibacillus laterosporus was investigated. Maximum siderophore production was determined as 50 % at 48 h in the metal-free growth media. In addition, maximum siderophore production was determined for various heavy metals including 5 μM Hg2+, 0.5 mM Ni2+, 0.1 mM Co2+, and 2.5 μM Fe2+. Intracellular uptake of the mercury was also measured using optical emission spectroscopy and compared with siderophore production values of the B. laterosporus. The maximum biosorption of mercury was measured to be 40% in 5 μM Hg2+-containing media at 48 h of incubation. The results show that siderophore production is affected by uptake of various metals, and are usable for removing of heavy metals from environmental habitats.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78825017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0