Hacettepe Journal of Biology and Chemistry最新文献

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Polyurethanes: Design, synthesis and structure-property behavior of versatile materials 聚氨酯:多用途材料的设计、合成和结构性能行为
Hacettepe Journal of Biology and Chemistry Pub Date : 2020-10-14 DOI: 10.15671/hjbc.809902
I. Yilgor, E. Yilgor
{"title":"Polyurethanes: Design, synthesis and structure-property behavior of versatile materials","authors":"I. Yilgor, E. Yilgor","doi":"10.15671/hjbc.809902","DOIUrl":"https://doi.org/10.15671/hjbc.809902","url":null,"abstract":"Polyurethanes are one of the most important classes of polymeric materials. This is mainly due to the availability of a very large number of inherently different starting materials that allows the design and synthesis of polyurethane based materials with a wide range of properties for numerous applications. In this short review, important physical and chemical factors and parameters that have a significant effect on the properties of polyurethanes are discussed. Critical contribution of hydrogen bonding on the structure-morphology-property behavior of these materials was emphasized by both experimental data and molecular simulation studies. Influence of the chemical structures, solubility parameters and molecular weights of the soft and hard segments on morphology and properties were discussed. Important issues regarding the reaction chemistry, synthetic method used and thermal history on structure and performance of polyurethanes were explained. We hope this article, which is prepared to celebrate the 100th anniversary of Polymer Science, will be useful to those who are newcomers to the field, but also to the experienced researchers to better understand the structure-property behavior of polyurethanes and tailor-design novel structures for various applications.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80194016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Electrospun Nanofibers for Wound Dressing and Tissue Engineering Applications 静电纺纳米纤维在伤口敷料和组织工程中的应用
Hacettepe Journal of Biology and Chemistry Pub Date : 2020-10-14 DOI: 10.15671/hjbc.789186
B. Balusamy, A. Senthamizhan, T. Uyar
{"title":"Electrospun Nanofibers for Wound Dressing and Tissue Engineering Applications","authors":"B. Balusamy, A. Senthamizhan, T. Uyar","doi":"10.15671/hjbc.789186","DOIUrl":"https://doi.org/10.15671/hjbc.789186","url":null,"abstract":"Electrospinning has received tremendous attention in the fabrication of nanofibrous scaffolds over recent years and employed in different biomedical applications because of their biomimetic nature. Especially, the electrospun nanofibers exhibit several beneficial features including natural extracellular matrix (ECM), interconnected pores, large surface area, ease of functionalization and mechanical performance that holds huge importance in influencing the cell adhesion, differentiation and proliferation behaviour. To date, acknowledging the wide range of beneficial features, the electrospun nanofibers have been used in wound dressing and tissue engineering applications. This review summarizes various efforts have been made in these areas with several representative examples indicating use of various materials and approaches. Further the concerns for future direction regard to clinical phase transfer has been discussed.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76061981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
in vitro EVALUATION OF NAPROXEN METABOLITE, 6-O-DESMETHYLNAPROXEN ON A MOUSE CONNECTIVE TISSUE FIBROBLAST CELLS 萘普生代谢物6- o -去甲基萘普生对小鼠结缔组织成纤维细胞的体外评价
Hacettepe Journal of Biology and Chemistry Pub Date : 2020-10-08 DOI: 10.15671/hjbc.680564
Esin Akbay, Y. D. Aracagök, Ali Hakan Göker, M. A. Onur, N. Cihangir
{"title":"in vitro EVALUATION OF NAPROXEN METABOLITE, 6-O-DESMETHYLNAPROXEN ON A MOUSE CONNECTIVE TISSUE FIBROBLAST CELLS","authors":"Esin Akbay, Y. D. Aracagök, Ali Hakan Göker, M. A. Onur, N. Cihangir","doi":"10.15671/hjbc.680564","DOIUrl":"https://doi.org/10.15671/hjbc.680564","url":null,"abstract":"The aim of this in vitro study was assessment of the cytotoxic level of O-desmethylnaproxen. A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and O-desmethylnaproxen in different concentrations for 24and 48hours. Cell viability was tested by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphnyltetrazolium bromide (MTT), while apoptosis was determined by acridine orange/propidium iodide (AO/PI) double staining method. The control group was free from any agent (Dulbecco’s Modified Eagle’s Medium (DMEM) only) and accepted as 100% cell viability. The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of O-desmethylnaproxen (0.7 μg/mL) while none of naproxen concentration was caused 50% cell death. Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87326131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial Activity of Hemolymph and Venom Obtained from Some Scorpion Species 几种蝎子血淋巴和毒液的抗菌活性研究
Hacettepe Journal of Biology and Chemistry Pub Date : 2020-10-06 DOI: 10.15671/hjbc.646190
H. Koç, C. Avşar, Yusuf Bayrakcı
{"title":"Antimicrobial Activity of Hemolymph and Venom Obtained from Some Scorpion Species","authors":"H. Koç, C. Avşar, Yusuf Bayrakcı","doi":"10.15671/hjbc.646190","DOIUrl":"https://doi.org/10.15671/hjbc.646190","url":null,"abstract":"The aim of this study was to determine the antibacterial and antifungal activities of hemolymph and venom obtained from some scorpion species. Venom samples were obtained by electrical stimulation method from five different scorpion species. Again, hemolymph fluids were obtained from five different scorpion species. Antimicrobial activities of hemolymph and venom diluted 1:1 with sterile saline were tested against a total of 10 microorganisms including seven bacteria and three yeasts using disc diffusion method. Venom samples of Protoiurus kraepelini showed inhibition zones of 9-20 mm on all microorganisms tested. Venom from this species has the highest antibacterial activity against Bacillus subtilis. The venom samples of Aegaeobuthus gibbosus were effective (7-12 mm) on all microorganisms except Enterococcus faecalis and Micrococcus luteus. In addition, it was found that venoms obtained from Androctonus crassicauda, Hottentotta saulcyi and Leiurus abdullahbayrami have different levels of antibacterial effect but no antifungal effect against Candida species. Scorpion hemolymphs have been shown to have similar effects against Gram-positive and Gram-negative bacteria. Besides, the obtained hemolymphs have been found to have low effect (7-9 mm) on microorganisms.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81582102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of bacterial PHBV-conduit used for nerve regeneration on oxidative stress parameters in rats 细菌phbv -导管用于神经再生对大鼠氧化应激参数的影响
Hacettepe Journal of Biology and Chemistry Pub Date : 2020-09-28 DOI: 10.15671/HJBC.617783
M. Demirbilek, M. Demirbilek, E. Erdal, M. Sakar, G. Bozkurt
{"title":"Effects of bacterial PHBV-conduit used for nerve regeneration on oxidative stress parameters in rats","authors":"M. Demirbilek, M. Demirbilek, E. Erdal, M. Sakar, G. Bozkurt","doi":"10.15671/HJBC.617783","DOIUrl":"https://doi.org/10.15671/HJBC.617783","url":null,"abstract":"in or its on antioxidant by electrospinning and conduits formed by using the nanofiber the of a 1 cm gap in the peritoneal PHBV conduits sacri 17th week after the operations. (MDA), oxidation protein products (AOPP), glutathione and superoxide dismutase of as as surrounding of conduits and serums were measured. Compared to control groups, MDA, AOPP and GSH levels and SOD activites in all graft group serums showed a significant increase, while only MDA and AOPP levels in tissues were statistically higher. Therefore, these findings suggest that PHBV nerve graft used for sciatic nerve defects may lead to oxidative stress in rats.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75197350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits 石竹叶和茎DNA:四种商品化提取试剂盒DNA条形码分析的比较研究
Hacettepe Journal of Biology and Chemistry Pub Date : 2020-09-28 DOI: 10.15671/HJBC.663505
Z. U. Aydın, M. Şenova, M. Koch, A. Dönmez
{"title":"DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits","authors":"Z. U. Aydın, M. Şenova, M. Koch, A. Dönmez","doi":"10.15671/HJBC.663505","DOIUrl":"https://doi.org/10.15671/HJBC.663505","url":null,"abstract":"F our widely used DNA extraction kits, GeneAll GenEx TM Plant kit, GeneMATRIX Plant & Fungi DNA Purification kit, DNeasy Plant kit and E.Z.N.A. Plant DNA kit, were compared using leaf and stem tissues of Dianthus specimens to isolate high yield and quality genomic DNA for DNA barcoding. Traditionally, leaves are used as a main source of genomic DNA but extracting DNA from Dianthus leaves might be more difficult because of sclerenchymatic tissues. Genomic DNA was extracted based on manufacturers’ protocols and amplified for seven barcode regions by PCR. DNA concentrations were measured using fluorometer and the quality of DNA and PCR products were showed on agarose gel. Amplification results were evalu-ated in terms of Polymerase Chain Reaction (PCR) success for DNA barcoding research. Additionally, comparisons between the efficiency of commercial kits were discussed in terms of cost, labor and time-consuming. Results presented here, DNA extraction carried out from both stem and leaves of Dianthus specimens successfully. Although, all kits performed well for PCR amplification, GeneMATRIX was found the best efficient kit for isolating high quality DNA of Dianthus specimens used in the study for leaf and stem tissues.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86630427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Effect of Slope on the Analysis of Forest Fire Risk 坡度对森林火险分析的影响
Hacettepe Journal of Biology and Chemistry Pub Date : 2020-09-03 DOI: 10.15671/hjbc.753080
Uğur Baltaci, Feriha Yıldırım
{"title":"Effect of Slope on the Analysis of Forest Fire Risk","authors":"Uğur Baltaci, Feriha Yıldırım","doi":"10.15671/hjbc.753080","DOIUrl":"https://doi.org/10.15671/hjbc.753080","url":null,"abstract":"Forest fires are mainly caused by human activities. Therefore, the forest fire risk in an area is under the influence of human activities that may cause forest fires if the vegetation and topographic features are also suitable. While evaluating the effect of slope on forest fire risk in our study, It has been analyzed with geographical information systems (GIS) the starting points of forest fires have occurred in Turkey in the last 10 years. Thus, an objective assessment try to has been made about the effect of slope on forest fire risk. According to the analysis results, 40.53% of forest fires occur in Turkey the range of 0-10% slope. In addition, 87.16% of these fires occur at 0-30% slope. As a result, contrary to the general belief, it has been determined that as the slope increases, the risk of forest fire decreases. The slope affects the fire inversely proportional, On the other hand, it affects the forest fire danger in direct proportion.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90381234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Meroplankton Composition of a low productive Lagoon System (Köyceğiz-Dalyan): temporally but not spatially variations 低生产力泻湖系统的浮游生物组成(Köyceğiz-Dalyan):时间而非空间变化
Hacettepe Journal of Biology and Chemistry Pub Date : 2020-08-13 DOI: 10.15671/hjbc.753642
N. Kİllİ
{"title":"Meroplankton Composition of a low productive Lagoon System (Köyceğiz-Dalyan): temporally but not spatially variations","authors":"N. Kİllİ","doi":"10.15671/hjbc.753642","DOIUrl":"https://doi.org/10.15671/hjbc.753642","url":null,"abstract":"S easonal meroplankton abundances of Köyceğiz-Dalyan Lagoon System were examined in this study. Also, changes in the diversity and abundance of meroplanktonic larvae because of the different two salinity layers of the system were investigated. Meroplankton samples were taken by Unesco standart plankton net with 200 micrometer mesh size horizon-tally from three stations. Temporal and spatial differences in the abundance of meroplankton groups were examined by permutational multivariate analysis of variance (PERMANOVA). Environmental parameters which are temperature, salinity and dissolved oxygen were measured from the surface. Meroplankton groups of the study area were nauplius, zoea, cyprid larva, stomatopod larva, hydrozoan larva, ephyra, polychaeta larva, fish egg, fish larva and gastropod larva. Meroplankton and holoplankton abundances showed two peaks in April and October. There were statistically significant differences in the abundance of meroplankton amongst seasons especially between summer-winter and winter-spring. In October and November Mediterranean water enters the Köyceğiz-Dalyan Lagoon System through the bottom of the system, whereas in winter and spring, the lagoon and coastal zone were under the influence of Lake Köyceğiz.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89942493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
α-Amylase Inhibition Properties of Bee Pollen and Bee Bread (Perga) 蜂花粉和蜂面包对α-淀粉酶的抑制作用
Hacettepe Journal of Biology and Chemistry Pub Date : 2020-08-12 DOI: 10.15671/hjbc.774901
M. Keskin, Aslı Özkök
{"title":"α-Amylase Inhibition Properties of Bee Pollen and Bee Bread (Perga)","authors":"M. Keskin, Aslı Özkök","doi":"10.15671/hjbc.774901","DOIUrl":"https://doi.org/10.15671/hjbc.774901","url":null,"abstract":"Bee pollen is a valuable bee product with its nutritional and bioactive values according to its protein, lipid, amino acid, vitamin and phenolic content. When bee pollen is converted into bee bread, it becomes more easily digestible in the human digestive system, because of the partial breakdown of the pollen wall, the exine layer, with the effect of fermentation. Therefore, bee bread has a richer nutrient content available than bee pollen. Diabetes mellitus is a metabolic and degenerative disease with long-term effects and inhibition of α-amylase is important for the treatment of this disease. In this study, methanol extract of pollen and perga samples was prepared. Total phenolic content, antioxidant capacity and α-amylase inhibition activity of the extract was determined. Total phenolic content and antioxidant capacity of pollen and perga extract was determined as 5.57-6.93 mg GAE/g and 74.56-97.66 μmol FeSO4.7H2O/g sample, respectively. IC50 value of bee pollen and perga for α-amylase inhibition was found to be 4.21 and 3.57 mg/mL, respectively. It could be concluded that perga may be more effective on Diabetes mellitus.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80860278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Designing of alternative polymeric nano-chelator for treatment in acute iron poisoning by molecular imprinting approach 分子印迹法治疗急性铁中毒的聚合物纳米螯合剂设计
Hacettepe Journal of Biology and Chemistry Pub Date : 2020-05-20 DOI: 10.15671/hjbc.723455
Veyis Karakoc
{"title":"Designing of alternative polymeric nano-chelator for treatment in acute iron poisoning by molecular imprinting approach","authors":"Veyis Karakoc","doi":"10.15671/hjbc.723455","DOIUrl":"https://doi.org/10.15671/hjbc.723455","url":null,"abstract":"T he aim of this study is to develop an alternative polymeric chelating agent for rapid and selectively removal with high capacity of iron (Fe 3 ) + ions from the gastrointestinal (GI) tract for the oral treatment of acute iron poisoning. For this purpose, Fe 3+ imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(l)- glutamic acid) (HEMA-MAGA) nanoparticles were synthesized by surfactant free emülsiyon polymerization. Molecular imprinting (MIP) technique was used to enhance the selectivity of nanoparticles. Due to being carboxyl and amide groups on the MAGA monomer, it was chosen as a chelating agent for Fe 3+ ions. Before the synthesizing of Fe 3+ imprinted polymer, Fe 3+ ions were complexed with (N-methacryloyl-(l)-glutamic acid) MAGA and then Fe 3+ imprinted nanoparticles were synthesized in the presence of this Fe 3+ -MAGA complexes. Poly(HEMA-MAGA) nanoparticles were characterized by infrared spectroscopy (FTIR), atomic force microscopy (AFM). Average particle size and size distribution also determined by zeta sizer. The specific surface area and mead diameter of the Fe 3+ imprinted poly(HEMA-MAGA) nanoparticles was 895 m 2 .g -1 and 95.3 nm, respectively. The maximum Fe 3+ ions binding capacity of the poly(HEMA-MAGA) nanoparticles at pH:4.0 were 206.4 mg.g -1 nanoparticles in intestinal mimicking solution(IMS). Fe 3+ removal performance of the Fe 3+ imprinted poly(HEMA-MAGA) nanoparticles with presence of other ions, optimum medium pH, temperature and equilibrium binding time were also investigated. Fe 3+ removal studies were performed in both aqueous solution and intestinal mimicking solution. The results indicate that Fe 3+ imprinted poly(HEMA-MAGA) nanoparticles is an alternative chelating agent for the selective Fe 3+ ions removal in a short time and with very high capacity.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"540 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78883816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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