Esin Akbay, Y. D. Aracagök, Ali Hakan Göker, M. A. Onur, N. Cihangir
{"title":"萘普生代谢物6- o -去甲基萘普生对小鼠结缔组织成纤维细胞的体外评价","authors":"Esin Akbay, Y. D. Aracagök, Ali Hakan Göker, M. A. Onur, N. Cihangir","doi":"10.15671/hjbc.680564","DOIUrl":null,"url":null,"abstract":"The aim of this in vitro study was assessment of the cytotoxic level of O-desmethylnaproxen. A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and O-desmethylnaproxen in different concentrations for 24and 48hours. Cell viability was tested by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphnyltetrazolium bromide (MTT), while apoptosis was determined by acridine orange/propidium iodide (AO/PI) double staining method. The control group was free from any agent (Dulbecco’s Modified Eagle’s Medium (DMEM) only) and accepted as 100% cell viability. The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of O-desmethylnaproxen (0.7 μg/mL) while none of naproxen concentration was caused 50% cell death. Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"24 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"in vitro EVALUATION OF NAPROXEN METABOLITE, 6-O-DESMETHYLNAPROXEN ON A MOUSE CONNECTIVE TISSUE FIBROBLAST CELLS\",\"authors\":\"Esin Akbay, Y. D. Aracagök, Ali Hakan Göker, M. A. Onur, N. Cihangir\",\"doi\":\"10.15671/hjbc.680564\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The aim of this in vitro study was assessment of the cytotoxic level of O-desmethylnaproxen. A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and O-desmethylnaproxen in different concentrations for 24and 48hours. Cell viability was tested by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphnyltetrazolium bromide (MTT), while apoptosis was determined by acridine orange/propidium iodide (AO/PI) double staining method. The control group was free from any agent (Dulbecco’s Modified Eagle’s Medium (DMEM) only) and accepted as 100% cell viability. The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of O-desmethylnaproxen (0.7 μg/mL) while none of naproxen concentration was caused 50% cell death. Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.\",\"PeriodicalId\":12939,\"journal\":{\"name\":\"Hacettepe Journal of Biology and Chemistry\",\"volume\":\"24 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-10-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hacettepe Journal of Biology and Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15671/hjbc.680564\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hacettepe Journal of Biology and Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15671/hjbc.680564","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
in vitro EVALUATION OF NAPROXEN METABOLITE, 6-O-DESMETHYLNAPROXEN ON A MOUSE CONNECTIVE TISSUE FIBROBLAST CELLS
The aim of this in vitro study was assessment of the cytotoxic level of O-desmethylnaproxen. A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and O-desmethylnaproxen in different concentrations for 24and 48hours. Cell viability was tested by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphnyltetrazolium bromide (MTT), while apoptosis was determined by acridine orange/propidium iodide (AO/PI) double staining method. The control group was free from any agent (Dulbecco’s Modified Eagle’s Medium (DMEM) only) and accepted as 100% cell viability. The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of O-desmethylnaproxen (0.7 μg/mL) while none of naproxen concentration was caused 50% cell death. Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.