{"title":"al2o3np / cpe中ua电化学性质的研究及血清中ua伏安法测定方法的建立","authors":"Rüveyda Üver, F. Öztürk","doi":"10.15671/hjbc.1225829","DOIUrl":null,"url":null,"abstract":"The electrochemical properties of uric acid (UA) were determined by cyclic voltammetry (CV) and square wave voltammetry (SWV) at a carbon paste electrode modified with aluminum oxide nanoparticles (Al2O3NP/CPE) in 0.05 M pH 5.0 phosphate buffer. The adsorption properties of the molecule on Al2O3NP/CPE were investigated and the number of electrons transferred in the electrode reaction was calculated. A new voltammetric method for the determination of UA in human serum was also proposed. The linear operating range and limit of detection (LOD) of the method were found to be 0.1µM-230 µM and 0.1µM, respectively. The developed method for UA in serum showed high reliability, reproducibility, accuracy and precision.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"288 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"INVESTIGATION OF ELECTROCHEMICAL PROPERTIES OF UA AT AL2O3NP/CPE AND DEVELOPMENT OF VOLTAMMETRIC METHOD FOR ITS DETERMINATION IN SERUM\",\"authors\":\"Rüveyda Üver, F. Öztürk\",\"doi\":\"10.15671/hjbc.1225829\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The electrochemical properties of uric acid (UA) were determined by cyclic voltammetry (CV) and square wave voltammetry (SWV) at a carbon paste electrode modified with aluminum oxide nanoparticles (Al2O3NP/CPE) in 0.05 M pH 5.0 phosphate buffer. The adsorption properties of the molecule on Al2O3NP/CPE were investigated and the number of electrons transferred in the electrode reaction was calculated. A new voltammetric method for the determination of UA in human serum was also proposed. The linear operating range and limit of detection (LOD) of the method were found to be 0.1µM-230 µM and 0.1µM, respectively. The developed method for UA in serum showed high reliability, reproducibility, accuracy and precision.\",\"PeriodicalId\":12939,\"journal\":{\"name\":\"Hacettepe Journal of Biology and Chemistry\",\"volume\":\"288 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hacettepe Journal of Biology and Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15671/hjbc.1225829\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hacettepe Journal of Biology and Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15671/hjbc.1225829","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
采用循环伏安法(CV)和方波伏安法(SWV)测定了尿酸(UA)在0.05 M pH 5.0磷酸盐缓冲液中以氧化铝纳米粒子(Al2O3NP/CPE)修饰的碳膏电极上的电化学性质。研究了该分子在Al2O3NP/CPE上的吸附性能，并计算了电极反应中转移的电子数。提出了一种新的测定人血清中UA的伏安法。该方法的线性工作范围为0.1µM ~ 230µM，检出限为0.1µM。该方法具有较高的可靠性、重复性、准确度和精密度。
INVESTIGATION OF ELECTROCHEMICAL PROPERTIES OF UA AT AL2O3NP/CPE AND DEVELOPMENT OF VOLTAMMETRIC METHOD FOR ITS DETERMINATION IN SERUM
The electrochemical properties of uric acid (UA) were determined by cyclic voltammetry (CV) and square wave voltammetry (SWV) at a carbon paste electrode modified with aluminum oxide nanoparticles (Al2O3NP/CPE) in 0.05 M pH 5.0 phosphate buffer. The adsorption properties of the molecule on Al2O3NP/CPE were investigated and the number of electrons transferred in the electrode reaction was calculated. A new voltammetric method for the determination of UA in human serum was also proposed. The linear operating range and limit of detection (LOD) of the method were found to be 0.1µM-230 µM and 0.1µM, respectively. The developed method for UA in serum showed high reliability, reproducibility, accuracy and precision.
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