Growth factors最新文献_第7页

Successful production of human epidermal growth factor in tobacco chloroplasts in a biologically active conformation. 在烟草叶绿体中成功生产出具有生物活性构象的人类表皮生长因子。

IF 1.8 4区生物学

Growth factors Pub Date : 2023-02-01 Epub Date: 2022-12-01 DOI: 10.1080/08977194.2022.2150187

Yunpeng Wang, Jieying Fan, Niaz Ahmad, Wen Xin, Zhengyi Wei, Shaochen Xing

{"title":"Successful production of human epidermal growth factor in tobacco chloroplasts in a biologically active conformation.","authors":"Yunpeng Wang, Jieying Fan, Niaz Ahmad, Wen Xin, Zhengyi Wei, Shaochen Xing","doi":"10.1080/08977194.2022.2150187","DOIUrl":"10.1080/08977194.2022.2150187","url":null,"abstract":"Human epidermal growth factor (hEGF) is an important therapeutic compound with multiple applications particularly in pharmaceutical industry. Human EGF has already been expressed in different expression systems, however, the production of hEGF with bioactivity in chloroplasts has not been successful so far. In this study, we expressed a 6 × His-tagged hEGF in tobacco chloroplasts in its native conformation for the potential of large-scale production of hEGF for industrial applications. Several transplastomic plant lines were obtained, which were screened by PCR (polymerase chain reaction) using primers specific to selectable gene aadA, hEGF- and GFP-coding sequences that were included in the chloroplast expression vector. The selected lines were confirmed to be homoplasmic by PCR verification and Southern blot analysis. Immunoblotting assays of homoplasmic lines using antibodies raised against hEGF confirmed the accumulation of hEGF in transplastomic plants and the ELISA results demonstrated the expression levels of hEGF were between 0.124% and 0.165% of the total soluble proteins (TSP), namely, 23.16-25.77 ng/g of the fresh weight. In terms of activity, the data from cell proliferation and elongation assays showed that the tobacco-derived recombinant hEGF was as bioactive as its commercial counterpart. To our knowledge, this is the first report of recombinant production of hEGF with native bioactivity form in the chloroplast stroma. Overall, our results demonstrate the potential of higher plant chloroplasts for the production of a human therapeutic, hEGF, in an active conformation.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"41 1","pages":"20-31"},"PeriodicalIF":1.8,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9127734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effects of propofol on IGF-1 activity and cell behaviour in the GES 1 mucosal cell model. 异丙酚对GES -1粘膜细胞模型中IGF-1活性和细胞行为的影响

IF 1.8 4区生物学

Growth factors Pub Date : 2023-02-01 DOI: 10.1080/08977194.2022.2150189

Chai Hua, Peng Wenyong, Zhu Zhongquan, Xiong Chang, Jin Xiayun

引用次数: 0

HIVEP3 as a potential prognostic factor promotes the development of acute myeloid leukemia. HIVEP3作为一个潜在的预后因子促进急性髓系白血病的发展。

IF 1.8 4区生物学

Growth factors Pub Date : 2023-02-01 DOI: 10.1080/08977194.2022.2158329

Yanfei Tang, Guangtao Xu, Bo Hu, Yuzhang Zhu

{"title":"HIVEP3 as a potential prognostic factor promotes the development of acute myeloid leukemia.","authors":"Yanfei Tang, Guangtao Xu, Bo Hu, Yuzhang Zhu","doi":"10.1080/08977194.2022.2158329","DOIUrl":"https://doi.org/10.1080/08977194.2022.2158329","url":null,"abstract":"Acute myeloid leukemia (AML) is a common malignancy worldwide. Human immune deficiency virus type 1 enhancer-binding protein 3 (HIVEP3) was verified to play a vital role in types of cancers. However, the functional role of HIVEP3 in AML was rarely reported. In this study, CCK-8, colony formation assay, flow cytometry, and Trans-well chamber experiments were applied for detecting cell proliferation, apoptosis, and invasion in AML cells. The expression of proteins related to TGF-β/Smad signaling pathway was determined by western blot. Our data showed that the expression level of HIVEP3 was closely related to the risk classification and prognosis of AML patients. Moreover, HIVEP3 was highly expressed in AML patients and cells. Knockdown of HIVEP3 significantly repressed cell proliferation invasion, and enhanced cell apoptosis in HL-60 and THP-1 cells. In addition, HIVEP3 donwreglation could inhibit the TGF-β/Smad signaling pathway. TGF-β overexpression could reverse the inhibition effects of HIVEP3 knockdown on AML development and the TGF-β/Smad signaling pathway. These findings indicated that HIVEP3 contributed to the progression of AML via regulating the TGF-β/Smad signaling pathway and had a prognostic value for AML.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"41 1","pages":"43-56"},"PeriodicalIF":1.8,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9130373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Platelet lysate and tendon healing: comparative analysis of autologous frozen-thawed PRP and ketorolac tromethamine in the treatment of patients with rotator cuff tendinopathy. 血小板裂解液与肌腱愈合:自体冻融PRP与丙酮酸三聚氰胺治疗肩袖肌腱病变的比较分析。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2093198

Raha Markazi, Mohammad Sadegh Soltani-Zangbar, Majid Zamani, Shadi Eghbal-Fard, Roza Motavalli, Amin Kamrani, Sanam Dolati, Majid Ahmadi, Leili Aghebati-Maleki, Amir Mehdizadeh, Fariba Eslamian, Alireza Pishgahi, Mehdi Yousefi

{"title":"Platelet lysate and tendon healing: comparative analysis of autologous frozen-thawed PRP and ketorolac tromethamine in the treatment of patients with rotator cuff tendinopathy.","authors":"Raha Markazi, Mohammad Sadegh Soltani-Zangbar, Majid Zamani, Shadi Eghbal-Fard, Roza Motavalli, Amin Kamrani, Sanam Dolati, Majid Ahmadi, Leili Aghebati-Maleki, Amir Mehdizadeh, Fariba Eslamian, Alireza Pishgahi, Mehdi Yousefi","doi":"10.1080/08977194.2022.2093198","DOIUrl":"https://doi.org/10.1080/08977194.2022.2093198","url":null,"abstract":"Platelet-rich blood derivatives are being nowadays increasingly used in the treatment of tendon-related pathologies as a rich source of growth factors. We sought to ascertain if local application of platelet lysate (PL) to augment rotator cuff repair ameliorates patient outcomes compared to ketorolac tromethamine treated group. A total of forty patients, with clinical diagnosis of Rotator Cuff Tendinopathy were randomized to receive sub acromial injections of PL every week for a total of 3 injections and two injection of ketorolac tromethamine once every two weeks. Subjective assessments included VAS, SPADI and shoulder range of motion were assessed at baseline and at 1 and 6 months after injection. Taking both control and PL groups, it was vividly seen that the outcomes were identical at the initial state, as well as the short-term one; whereas, when considering the 6-month period, there is a seemingly remarkable superiority in PL group in all parameters.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"40 5-6","pages":"163-174"},"PeriodicalIF":1.8,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10793284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

MiR-200c regulates invasion, proliferation and EMT of anaplastic thyroid cancer cells by targeting parathyroid hormone like hormone. MiR-200c通过靶向甲状旁腺激素样激素调控间变性甲状腺癌细胞的侵袭、增殖和EMT。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2108809

Yan Zhang, Yuanyuan Duan, Chenguang Wu, Wen Peng, Wenyu Chen, Li Wang, Zhaoqun Deng

{"title":"MiR-200c regulates invasion, proliferation and EMT of anaplastic thyroid cancer cells by targeting parathyroid hormone like hormone.","authors":"Yan Zhang, Yuanyuan Duan, Chenguang Wu, Wen Peng, Wenyu Chen, Li Wang, Zhaoqun Deng","doi":"10.1080/08977194.2022.2108809","DOIUrl":"https://doi.org/10.1080/08977194.2022.2108809","url":null,"abstract":"This study aimed to explore the specific effect of miR-200c in anaplastic thyroid cancer (ATC). Hth74 and ARO cell lines were used. Proliferation, invasion, and colony formation activities of Hth74 and ARO cell lines affected by miR-200c were studied. Expression of epithelial-to-mesenchymal transition (EMT) markers (E-cadherin, N-cadherin, Slug, and Snail) in the Hth74 and ARO cell lines were validated by western blot and qRT-PCR. In addition, the regulation of the parathyroid hormone-like hormone (PTHLH) by miR-200c was assessed. Overexpression of miR-200c inhibited the invasion, proliferation, and colony formation of the ATC cell lines, whereas its downregulation achieved the opposite results. PTHLH was found to be regulated negatively by miR-200c through a miR-200c binding site within the 3'-UTR of PTHLH. miR-200c repressed the proliferation, invasion, and EMT process of cells in ATC cell lines by targeting PTHLH post-transcriptionally, which indicates that miR-200c may be a potential target for the treatment of ATC.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"40 5-6","pages":"175-185"},"PeriodicalIF":1.8,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10803226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased circulating TGF-β1 is associated with impairment in NK cell effector functions in metastatic melanoma patients. 在转移性黑色素瘤患者中，循环TGF-β1升高与NK细胞效应物功能受损相关。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2124915

Katarina Mirjačić Martinović, Ana Vuletić, Emina Mališić, Tatjana Srdić-Rajić, Nevena Tišma Miletić, Nada Babović, Vladimir Jurišić

{"title":"Increased circulating TGF-β1 is associated with impairment in NK cell effector functions in metastatic melanoma patients.","authors":"Katarina Mirjačić Martinović, Ana Vuletić, Emina Mališić, Tatjana Srdić-Rajić, Nevena Tišma Miletić, Nada Babović, Vladimir Jurišić","doi":"10.1080/08977194.2022.2124915","DOIUrl":"https://doi.org/10.1080/08977194.2022.2124915","url":null,"abstract":"Transforming growth factor beta (TGF-β) plays a complex role in carcinogenesis. In 30 melanoma patients and 20 healthy controls (HC) we analysed functional and phenotypic characteristics of NK cells by Flow cytometry, gene expression of TGF-β1 in peripheral blood mononuclear cells by qPCR and serum and supernatant level of free TGF-β1 by ELISA. Melanoma patients had significantly higher serum level of circulatingTGF-β1 compared to HC, especially those with metastasis into the central nervous system (subclass M1d) and high LDH serum values. Melanoma patients compared to HC had significantly higher level of TGF-β1 gene in PBMC. TGF-β1 serum values negatively correlate with NK cell activity analysed by CD107a (degranulation marker), IFN-γ, NKG2D, and NKp46 in patients. Study shows the association of high level of TGF-β1 with NK cell inhibition in patients represents the main mechanism of tumour immune evasion. Targeting TGF-β may become an important cancer treatment for improving antitumor immunity.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"40 5-6","pages":"231-239"},"PeriodicalIF":1.8,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9344848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effects of penehyclidine hydrochloride on myocardial ischaemia-reperfusion injury in rats by inhibiting TLR4/MyD88/NF-κB pathway via miR-199a-3p. 盐酸戊乙奎醚通过miR-199a-3p抑制TLR4/MyD88/NF-κB通路对大鼠心肌缺血再灌注损伤的影响

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2109469

Ling Qiang Bai, Bin Zhe Wang, Qi Wei Liu, Wen Qiang Li, Hang Zhou, Xiao Yan Yang

{"title":"Effects of penehyclidine hydrochloride on myocardial ischaemia-reperfusion injury in rats by inhibiting TLR4/MyD88/NF-κB pathway via miR-199a-3p.","authors":"Ling Qiang Bai, Bin Zhe Wang, Qi Wei Liu, Wen Qiang Li, Hang Zhou, Xiao Yan Yang","doi":"10.1080/08977194.2022.2109469","DOIUrl":"https://doi.org/10.1080/08977194.2022.2109469","url":null,"abstract":"This study was to probe the role of penehyclidine hydrochloride (PHC) mediating the impact of toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-kappa B (NF-κB) signalling pathway on myocardial ischaemia-reperfusion injury (MI/RI) in rats through miR-199a-3p. The rat MI/RI model was established through ligating left anterior descending (LAD) coronary artery. PHC was injected preoperatively into the model rats, and injected with miR-199a-3p lentiviral vector or TLR4 antagonist (TAK-242). Next, cardiac function of rats was examined by echocardiography, and rat serum indicators, oxidative stress levels and inflammatory factors were detected. HE staining was applied to detect pathological tissue structure, TUNEL staining to detect apoptosis rate, qRCR and western blot to detect miR-199a-3p and TLR4/MyD88/NF-κB expressions in rat myocardial tissues. Dual luciferase reporter experiment was conducted to confirm the relationship between miR-199a-3p and TLR4. In conclusion, PHC suppresses TLR4/MyD88/NF-κB signalling pathway through miR-199a-3p, thereby improving MI/RI in rats.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"40 5-6","pages":"186-199"},"PeriodicalIF":1.8,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10797623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

High mobility group box 1 and Dickkopf-related protein 1 as biomarkers of glucose toxicity, atherogenicity, and lower β cell function in patients with type 2 diabetes mellitus. 高迁移率组盒1和dickkopf相关蛋白1作为2型糖尿病患者葡萄糖毒性、动脉粥样硬化性和低β细胞功能的生物标志物

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2126317

Hussein Kadhem Al-Hakeim, Qasim Jasim Al-Kaabi, Michael Maes

引用次数: 3

Matrine induces hepatocellular carcinoma apoptosis and represses EMT and stemness through microRNA-299-3p/PGAM1 axis. 苦参碱通过microRNA-299-3p/PGAM1轴诱导肝癌细胞凋亡，抑制EMT和干细胞。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2113073

BaoLin Wang, HuiHai Wang, Qin Zhao, Fei Lu, ZhenZhuang Yan, Fang Zhou, QingLun Su

{"title":"Matrine induces hepatocellular carcinoma apoptosis and represses EMT and stemness through microRNA-299-3p/PGAM1 axis.","authors":"BaoLin Wang, HuiHai Wang, Qin Zhao, Fei Lu, ZhenZhuang Yan, Fang Zhou, QingLun Su","doi":"10.1080/08977194.2022.2113073","DOIUrl":"https://doi.org/10.1080/08977194.2022.2113073","url":null,"abstract":"This study explored the impacts of matrine on hepatocellular carcinoma (HCC) cell growth, metastasis, epithelial-mesenchymal transition (EMT), and stemness through regulating the microRNA (miR)-299-3p/phosphoglycerate mutase 1 (PGAM1) axis. The association between miR-299-3p expression with the prognosis of HCC patients was studied. miR-299-3p and PGAM1 sequences were transfected into matrine-treated HCC cells, and cell proliferation, invasion, apoptosis, and stemness were detected, as well as protein expression of EMT- and stemness-related makers. The targeting relationship between miR-299-3p and PGAM1 was identified. Matrine elevated miR-299-3p expression, repressed proliferation, invasion, and anti-apoptosis of HCC cells, and constrained EMT and stemness in vitro. PGAM1 was a target of miR-299-3p. Repression of PGAM1 rescued the effects of miR-299-3p downregulation on HCC cells. Matrine stimulates HCC cell apoptosis and represses the process of EMT and stemness through the miR-299-3p/PGAM1 axis.","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":"40 5-6","pages":"200-211"},"PeriodicalIF":1.8,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10793795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Embryonic growth retardation and altered expression of IGF-II is reciprocal induced by phytocompounds during early gestation in mice. 胚胎生长迟缓和IGF-II的表达改变是由植物化合物在小鼠妊娠早期相互诱导的。

IF 1.8 4区生物学

Growth factors Pub Date : 2022-11-01 DOI: 10.1080/08977194.2022.2129018

Khamhee Wangsa, Krishnakshi Misra, Upasa Gowala, Indira Sarma, Purba Jyoti Saikia, Hirendra Nath Sarma

引用次数: 1