miR-146a抑制糖尿病视网膜病变血管内皮生长因子的表达及炎症反应

IF 1.8 4区生物学 Q4 CELL BIOLOGY

Growth factors Pub Date : 2022-05-23 DOI:10.1080/08977194.2022.2077732

Shichao Sun, Fu-Jun Wang, Yao Sun, L. Bai

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引用次数: 1

摘要

本研究旨在探讨miR-146a在糖尿病视网膜病变(DR)中的作用。健康对照(HC) 30例，2型糖尿病患者50例，DR患者48例。采集血液，检测miR-146a、血管内皮生长因子(VEGF)和三种炎症因子(NF-κB、IL-1β、TNF-α)的表达水平。此外，在高糖存在的情况下，用miR-146a模拟物或抑制剂处理ARPE-19细胞，以评估其体外效果。DR患者miR-146a水平最低，VEGF水平最高，炎症反应最严重。此外，在DR患者中，miR-146a水平分别与VEGF和三种炎症细胞因子的表达呈负相关。此外，在DR患者中，VEGF的表达与这三种炎症因子呈正相关。综上所述，miR-146a可以抑制DR中VEGF的表达和炎症。

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miR-146a suppresses the expression of vascular endothelial growth factor and inflammatory responses in diabetic retinopathy

Abstract This study was designed to explore the role of miR-146a in diabetic retinopathy (DR). 30 healthy control (HC), 50 patients with type 2 diabetes mellitus, and 48 DR patients were enrolled. Blood was collected and levels of miR-146a expression, vascular endothelial growth factor (VEGF), and three inflammatory cytokines (NF-κB, IL-1β, and TNF-α) were detected. Moreover, ARPE-19 cells were treated with miR-146a mimic or inhibitor in the presence of high glucose to evaluate its effect in vitro. DR patients had the lowest level of miR-146a and the highest level of VEGF as well as the most severe inflammation among the three groups. In addition, the miR-146a level was negatively correlated with the expression of VEGF and three inflammatory cytokines, respectively in DR patients. Moreover, VEGF expression was positively correlated with these three inflammatory cytokines in DR patients. In summary, miR-146a could inhibit VEGF expression and inflammation in DR.

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来源期刊

Growth factors 生物-内分泌学与代谢

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications