Icariin attenuates renal fibrosis in vivo and in vitro by inhibiting the Notch2/Hes-1 pathway

IF 1.8 4区生物学 Q4 CELL BIOLOGY

Growth factors Pub Date : 2022-04-15 DOI:10.1080/08977194.2022.2060094

Qiaoqin Zhang, Lei Xie, Linqing Jiang, Jiaqing Ni, Wenke Han, Xiu-hua Mi, Ping Wang

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引用次数: 2

Abstract

Abstract Chinese herbs were widely proposed as a novel approach for renal fibrosis. Icariin has been reported to be involved in a variety of diseases. Unilateral ureteral obstruction (UUO) is a popular experimental model of renal injury, which is often used in the study of renal fibrosis. A UUO mouse model was successfully constructed, and tubular injury and renal fibrosis were observed. Icariin treatment attenuated tubular injury and renal fibrosis in UUO mice. In addition, treatment with Icariin reduced the fibronectin, type I collagen and α-SMA levels in UUO mice. Furthermore, in a transforming growth factor (TGF)-β1-induced renal fibrosis cell model, icariin treatment also decreased fibronectin, type I collagen and α-SMA expression. Icariin treatment also reversed the enhanced migration of TGF-β1-induced HK-2 cells. These data indicated that icariin suppressed renal fibrosis in vivo and in vitro. Additionally, icariin treatment suppressed the Notch2/Hes-1 pathway in UUO mice and TGF-β1-treated HK-2 cells. In summary, this study found that icariin reduced renal fibrosis in vivo and in vitro by inhibiting the Notch2/Hes-1 pathway, which might help to improve therapies for renal fibrosis.

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Icariin通过抑制Notch2/Hes-1通路减轻体内外肾纤维化

摘要中草药被广泛认为是治疗肾纤维化的一种新方法。据报道，淫羊藿苷与多种疾病有关。单侧输尿管梗阻（UUO）是一种流行的肾损伤实验模型，常用于肾纤维化的研究。成功构建UUO小鼠模型，观察肾小管损伤和肾纤维化。Icariin治疗减轻UUO小鼠肾小管损伤和肾纤维化。此外，Icariin治疗降低了UUO小鼠的纤连蛋白、I型胶原和α-SMA水平。此外，在转化生长因子（TGF）-β1诱导的肾纤维化细胞模型中，icariin治疗还降低了纤连蛋白、I型胶原和α-SMA的表达。Icariin处理还逆转了TGF-β1诱导的HK-2细胞迁移增强。这些数据表明，icariin在体内和体外都能抑制肾纤维化。此外，icariin处理抑制了UUO小鼠和TGF-β1处理的HK-2细胞中的Notch2/Hes-1通路。总之，本研究发现，icariin通过抑制Notch2/Hes-1通路在体内和体外减少了肾纤维化，这可能有助于改善肾纤维化的治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Growth factors 生物-内分泌学与代谢

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications