Growth factors最新文献

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Activation of the non-canonical Wnt5a signaling pathway following optic nerve injury induces time-dependent changes in pro- and anti-inflammatory gene expression. 视神经损伤后非规范Wnt5a信号通路的激活可诱导促炎和抗炎基因表达的时间依赖性变化。
IF 1.7 4区 生物学
Growth factors Pub Date : 2025-07-31 DOI: 10.1080/08977194.2025.2539128
Alexander W Venanzi, Gabrielle A Albano, Paola E Parrales, Abigail S Hackam
{"title":"Activation of the non-canonical Wnt5a signaling pathway following optic nerve injury induces time-dependent changes in pro- and anti-inflammatory gene expression.","authors":"Alexander W Venanzi, Gabrielle A Albano, Paola E Parrales, Abigail S Hackam","doi":"10.1080/08977194.2025.2539128","DOIUrl":"https://doi.org/10.1080/08977194.2025.2539128","url":null,"abstract":"<p><p>Optic nerve (ON) injury leads to retinal ganglion cell (RGC) degeneration and axonal atrophy. Wnt ligands are embryonic growth factors that regulate cellular differentiation and survival. We recently demonstrated that canonical and non-canonical Wnt signaling induces RGC survival and axonal regrowth after optic nerve crush (ONC) injury in mouse. Here, we investigated whether the non-canonical Wnt5a ligand induces pro-regenerative inflammation after ONC. Mice were intravitreally injected with Wnt5a or saline during ONC and retina tissue was collected for QPCR and immunofluorescence. We demonstrated that expression of arginase 1, a marker of anti-inflammatory microglia, was upregulated by Wnt5a in injured retinas, whereas iNOS, a marker of neurotoxic microglia, was suppressed. Wnt5a also induced time-dependent changes in pro-inflammatory genes Gal3, TNFα, P2RY12 and IL-6 and the anti-inflammatory gene IL-27. These results indicate that Wnt5a is an immunomodulatory ligand in the retina after ONC injury.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"1-12"},"PeriodicalIF":1.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
VEGF ameliorates acute kidney injury by suppressing ferroptosis through activation of the ERK1/2-NRF2 pathway. VEGF通过激活ERK1/2-NRF2通路抑制铁下垂,改善急性肾损伤。
IF 1.8 4区 生物学
Growth factors Pub Date : 2025-07-22 DOI: 10.1080/08977194.2025.2533773
Xiangtian Liu, Yuqi Song, Weikun Tian, Liping Ye, Dongxiao Li, Meifeng Li, Xinghan Tian, Xiaoli Li
{"title":"VEGF ameliorates acute kidney injury by suppressing ferroptosis through activation of the ERK1/2-NRF2 pathway.","authors":"Xiangtian Liu, Yuqi Song, Weikun Tian, Liping Ye, Dongxiao Li, Meifeng Li, Xinghan Tian, Xiaoli Li","doi":"10.1080/08977194.2025.2533773","DOIUrl":"https://doi.org/10.1080/08977194.2025.2533773","url":null,"abstract":"<p><p>Vascular endothelial growth factor (VEGF) plays a crucial role in maintaining renal homeostasis. However, the precise impact of VEGF on ferroptosis in acute kidney injury (AKI) remains incompletely understood. This study aims to investigate the effects of VEGF on ferroptosis in the model of AKI and to elucidate the underlying mechanisms. We used C57BL mice and HK-2 cells to construct sepsis-associated AKI models. We assessed renal function, cell viability, and tissue levels of iron, malondialdehyde (MDA), and glutathione (GSH) in mice. Intracellular reactive oxygen species (ROS), mitochondrial membrane potential, and electron microscopy-detected cellular changes were also measured. Western blotting analyzed key ferroptosis-related proteins (SLC7A11, GPX4) and components of the ERK1/2-NRF2-GPX4 pathway. VEGF treatment significantly reduced oxidative stress by lowering ROS and MDA levels while increasing GSH. Additionally, VEGF165 activated the ERK1/2-NRF2 pathway, mitigating ferroptosis.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"1-11"},"PeriodicalIF":1.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Insulin-like growth factor 1 and insulin-like growth factor-binding protein 3 in central precocious puberty: a systematic review and meta-analysis. 胰岛素样生长因子1和胰岛素样生长因子结合蛋白3在中枢性性早熟中的作用:一项系统综述和荟萃分析
IF 1.8 4区 生物学
Growth factors Pub Date : 2025-05-01 Epub Date: 2025-07-02 DOI: 10.1080/08977194.2025.2521071
Yunyun Wu, Weili Zhao
{"title":"Insulin-like growth factor 1 and insulin-like growth factor-binding protein 3 in central precocious puberty: a systematic review and meta-analysis.","authors":"Yunyun Wu, Weili Zhao","doi":"10.1080/08977194.2025.2521071","DOIUrl":"10.1080/08977194.2025.2521071","url":null,"abstract":"<p><p>To identify distinct patterns of insulin-like growth factor-1 (IGF-1) and insulin-like growth factor-binding protein 3 (IGFBP-3) in girls with central precocious puberty (CPP), and to compare IGF-1 and IGFBP-3 levels in patients with CPP and precocious thelarche (PT).</p><p><p>A literature search of PubMed, Embase, and Scopus databases was done to identify observational studies. Newcastle-Ottawa Scale (NOS) was used to assess the quality of the studies. Pooled weighted mean difference (WMD) and 95% confidence intervals (CI) were reported.</p><p><p>Fourteen studies were included. As opposed to age-matched or similar-aged pre-pubertal girls, patients with CPP had significantly higher levels of IGF-1 and IGFBP-3. CPP was associated with higher levels of IGF-1 approaching statistical significance but similar levels of IGFBP-3 compared to PT.</p><p><p>Distinct hormonal patterns, such as elevated IGF-1 and IGFBP-3, were identified in patients with CPP, suggesting a potential diagnostic value of IGF-1 and IGFBP-3 levels.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"97-105"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mesencephalic astrocyte-derived neurotrophic factor: a potential diagnostic marker in human sepsis-associated lung injury. 中脑星形细胞源性神经营养因子:人类败血症相关肺损伤的潜在诊断标志物。
IF 1.8 4区 生物学
Growth factors Pub Date : 2025-05-01 Epub Date: 2025-06-19 DOI: 10.1080/08977194.2025.2522803
Yan Zhou, Ni Yao, Jing-Wen Zheng, Zi-Yao Wang, Li-Hong Wan, Yan Kang
{"title":"Mesencephalic astrocyte-derived neurotrophic factor: a potential diagnostic marker in human sepsis-associated lung injury.","authors":"Yan Zhou, Ni Yao, Jing-Wen Zheng, Zi-Yao Wang, Li-Hong Wan, Yan Kang","doi":"10.1080/08977194.2025.2522803","DOIUrl":"10.1080/08977194.2025.2522803","url":null,"abstract":"<p><p>Systemic inflammatory response syndrome (SIRS) commonly considered as the first step in the sepsis cascade. To evaluate the diagnostic value and potential mechanism of serum MANF in sepsis-associated lung injury (SALI), 15 adult SALI patients and 15 age- and sex-matched SIRS patients were enrolled to measure serum MANF levels by sandwich enzyme-linked immune-sorbent assay and the laboratory indexes including C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6) were collected. MANF and IL-6 levels showed significant differences between groups (P < 0.05), and the area under the curve (AUC) values of MANF and IL-6 were 0.822 and 0.815. The combination of MANF with IL-6 exhibited improved predictive accuracy for SALI. Furthermore, we found that there is a protein interaction network between MANF and all of these overlap targets between ferroptosis-related genes (FRGs) and SALI by bioinformatics analysis. MANF could be a promising biomarker for the differential diagnosis of SALI and SIRS.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"119-126"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An endothelial cell competition assay for determinants of the response to targeted anti-angiogenics. 内皮细胞竞争测定对靶向抗血管生成反应的决定因素。
IF 1.8 4区 生物学
Growth factors Pub Date : 2025-05-01 Epub Date: 2025-06-17 DOI: 10.1080/08977194.2025.2516463
Michael M Halford, Michael Y He, Nancy Amin, Sophie Paquet-Fifield, Marc G Achen, Elizabeth Vincan, Steven A Stacker
{"title":"An endothelial cell competition assay for determinants of the response to targeted anti-angiogenics.","authors":"Michael M Halford, Michael Y He, Nancy Amin, Sophie Paquet-Fifield, Marc G Achen, Elizabeth Vincan, Steven A Stacker","doi":"10.1080/08977194.2025.2516463","DOIUrl":"10.1080/08977194.2025.2516463","url":null,"abstract":"<p><p>ABSTRACT/SUMMARYAnti-angiogenics, inhibitors of pathological blood vessel growth, are an important class of targeted agent for the treatment of common cancers and ocular conditions. However, efficacy is compromised by the absence of biomarkers to guide patient selection or inform the management of resistance. We describe an assay for modified endothelial cell (EC) responses to the VEGF-A-neutralizing monoclonal antibody bevacizumab as part of a biomarker discovery program. ECs are transduced by lentivector expressing an experimental or non-silencing shRNA, each co-expressed with a different fluorescent protein. A 1:1 mixed cell population is then cultured with bevacizumab or control antibody under VEGF-A-dependent conditions. A normalized ratio of surviving cells, obtained by flow cytometry analysis, reflects EC resistance or sensitization to bevacizumab mediated by the experimental shRNA. With reagents prepared, the protocol takes 10 days and rigorously quantifies the impact of gene perturbation on the EC response to bevacizumab or other targeted anti-angiogenics.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"127-153"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oxaloacetate stimulates phosphorylation of epidermal growth factor receptor in epithelial cells in vitro. 草酰乙酸刺激体外上皮细胞中表皮生长因子受体的磷酸化。
IF 1.8 4区 生物学
Growth factors Pub Date : 2025-05-01 Epub Date: 2025-05-02 DOI: 10.1080/08977194.2025.2499634
Ye Kuang, Yuxiang Zhao, Zeyu Miao, Yang Xu, Qing Yang
{"title":"Oxaloacetate stimulates phosphorylation of epidermal growth factor receptor in epithelial cells in vitro.","authors":"Ye Kuang, Yuxiang Zhao, Zeyu Miao, Yang Xu, Qing Yang","doi":"10.1080/08977194.2025.2499634","DOIUrl":"10.1080/08977194.2025.2499634","url":null,"abstract":"<p><p>Oxaloacetate (OA) is a pivotal endogenous metabolite. Within our investigation, we ascertained that OA functions as an agonist for the epidermal growth factor receptor (EGFR), a key protagonist in the genesis of diverse tumours. We substantiated that escalating concentrations of OA initially enhanced the cellular viability of several cancer cells, followed by subsequent attenuation, which is similar to the effect of EGF. Furthermore, the protein phosphorylation profile in HepG2 cells exposed to OA closely paralleled that induced by epidermal growth factor (EGF). Additional findings underscored the capability of OA to induce the generation of EGFR dimers. Finally, our observations revealed that OA governs the activation of AKT and Erk, the typical downstream signalling proteins of EGFR. We postulate that the endogenous metabolite OA can function as either an agonist or inhibitor of EGFR at specific concentrations to modulate tumour proliferation, and provide new insights into the regulation of EGFR activation.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"106-118"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Various strategies to induce beta cell neogenesis: a comprehensive review for unravelling the potential future therapy for curing diabetes. 诱导β细胞新生的各种策略:全面回顾未来潜在的糖尿病治疗方法。
IF 1.8 4区 生物学
Growth factors Pub Date : 2025-05-01 Epub Date: 2025-05-21 DOI: 10.1080/08977194.2025.2508723
Anjali Patel, B Rajgopal, Manisha Jaiswal
{"title":"Various strategies to induce beta cell neogenesis: a comprehensive review for unravelling the potential future therapy for curing diabetes.","authors":"Anjali Patel, B Rajgopal, Manisha Jaiswal","doi":"10.1080/08977194.2025.2508723","DOIUrl":"10.1080/08977194.2025.2508723","url":null,"abstract":"<p><p>Pancreatic endocrine cells are categorized in to 5 types (alpha, beta, delta, pancreatic polypeptide cells and epsilon), which expresses glucagon, insulin, somatostatin, pancreatic polypeptide, and ghrelin, respectively. Several studies including lineage tracing in Ins2<sup>Akita</sup> diabetic mice have been done to investigate the identities of pancreatic endocrine cells which concludes, alpha cells have enormous plasticity, which enables them to be reprogrammed by specific transcription factors into insulin secreting beta like cells. Gene therapy has provided the beneficial outcome. Pdx1, MaFA and PAX4 (the transcription factors) in alpha cells can be over expressed which results in reprogramming the targeted alpha cells into beta cells. This trans-differentiation may be induced by infusing an adeno-associated virus (AAV) loaded with distinct transcription factors in the duct of pancreas. Several researches have demonstrated the successful restoration of enhanced insulin secretion in diabetes induced mice. Additionally ductal neurogenin3 (Ngn3), Sglt2 inhibitors, Igfbp1, GLP1 and several clinical and non-clinical agents has been postulated as a basis of beta cell neogenesis. Alpha cell owing to its high plasticity, on prolonged exposure to GABA reprogrammed into beta-like cell due to downregulation of Arx expression by GABA. The various approaches for beta cell neogenesis open a new window towards the establishment of novel gene therapy accession to treat diabetes. However, broad studies are still needed to improve and optimize this treatment methodology. The potentiality of endogenous pancreatic alpha cell to beta cell conversion methods and its outcomes are invigorating. This accomplishment is presently being under trial in non-human primates.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"69-96"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the mechanisms of Shugan-Jieyu-Jianpi formula against irritable bowel syndrome combined with non-alcoholic fatty liver disease by network pharmacology and experimental validation. 通过网络药理学和实验验证探讨疏肝解郁健脾方治疗肠易激综合征合并非酒精性脂肪肝的作用机制。
IF 1.8 4区 生物学
Growth factors Pub Date : 2025-02-01 Epub Date: 2025-02-22 DOI: 10.1080/08977194.2025.2467135
Xiaowen Yu, Xuan Chen, Jun Ouyang, Biao Xi, Defeng Wu, Ling Wei, Dongyu Xie, Yaxiang Shi
{"title":"Exploring the mechanisms of Shugan-Jieyu-Jianpi formula against irritable bowel syndrome combined with non-alcoholic fatty liver disease by network pharmacology and experimental validation.","authors":"Xiaowen Yu, Xuan Chen, Jun Ouyang, Biao Xi, Defeng Wu, Ling Wei, Dongyu Xie, Yaxiang Shi","doi":"10.1080/08977194.2025.2467135","DOIUrl":"10.1080/08977194.2025.2467135","url":null,"abstract":"<p><p>The study was aimed to investigate the clinical effect and mechanism of Shugan-Jieyu-Jianpi (SGJYJP) formula for the treatment of irritable bowel syndrome (IBS) combined with non-alcoholic fatty liver disease (NAFLD). The clinical efficacy of SGJYJP was evaluated in 54 patients with IBS-NAFLD. The potential molecular mechanism of SGJYJP formula was investigated by network pharmacology. Animal models were constructed to explore the related mechanism. From clinical studies, the total effective rate of patients in SGJYJP group was significantly higher than that in pinaverium group. The protein expression of TGFB1 was declined in IBS-NAFLD rats, together with the increased expression of PTGS2 and TNF, which was abolished by SGJYJP treatment. SGJYJP significantly reduced the expression of TNF signalling related molecules of TRAF2, caspase-8, and elevated the expression of Bcl-xl in IBS-NAFLD animal models. SGJYJP may exert therapeutic effect on IBS-NAFLD by targeting PTGS2, TGFB1, and TNF genes and TNF signalling.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"1-19"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression of DDX49 in breast cancer and its mechanism regulating the proliferation and metastasis of breast cancer cells. DDX49在乳腺癌中的表达及其调控乳腺癌细胞增殖转移的机制
IF 1.8 4区 生物学
Growth factors Pub Date : 2025-02-01 Epub Date: 2025-04-03 DOI: 10.1080/08977194.2025.2484007
Yuanbin Wang, Lijun Yang, Xiangli Li, Qing Yang, Ruimin Ma, Zhihao Wu
{"title":"Expression of DDX49 in breast cancer and its mechanism regulating the proliferation and metastasis of breast cancer cells.","authors":"Yuanbin Wang, Lijun Yang, Xiangli Li, Qing Yang, Ruimin Ma, Zhihao Wu","doi":"10.1080/08977194.2025.2484007","DOIUrl":"10.1080/08977194.2025.2484007","url":null,"abstract":"<p><p>DEAD-box RNA helicase (DDX) is linked to the invasion, drug resistance, proliferation, and epithelial-mesenchymal transition of tumour cells. This study examined the potential mechanisms of DDX49 in breast cancer. The expression of DDX49 in breast cancer tissues and cells was evaluated. The effects of DDX49 on proliferation, invasion, migration and apoptosis of breast cancer cells were evaluated. The expression of proteins associated with the JAK/STAT pathway was examined. A xenograft tumour model was established. DDX49 expression is elevated in breast cancer tissues and cell lines. shDDX49 suppressed the ability of breast cancer cells to proliferate, invade, and migrate, but promoted apoptosis. Conversely, overexpression of DDX49 exerted an opposite effect. The activation of the JAK-STAT signalling pathway is inhibited by the shDDX49. shDDX49 efficiently inhibits tumour growth in mice with breast cancer. shDDX49 may hinder the growth and spread of breast cancer cells by inhibiting the JAK-STAT pathway.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"45-55"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Super-activated platelet lysate promotes mesenchymal stem cell proliferation, migration, and chondrogenic differentiation: an in vitro study. 超活化血小板裂解液促进间充质干细胞增殖、迁移和软骨分化:一项体外研究。
IF 1.8 4区 生物学
Growth factors Pub Date : 2025-02-01 Epub Date: 2025-03-31 DOI: 10.1080/08977194.2025.2484614
Chunxiang Liu, Tianqi Zhang, Lingqi Meng, Ihsan Ullah, Heng Li, Fuge Sui, Yi Zhang
{"title":"Super-activated platelet lysate promotes mesenchymal stem cell proliferation, migration, and chondrogenic differentiation: an in vitro study.","authors":"Chunxiang Liu, Tianqi Zhang, Lingqi Meng, Ihsan Ullah, Heng Li, Fuge Sui, Yi Zhang","doi":"10.1080/08977194.2025.2484614","DOIUrl":"10.1080/08977194.2025.2484614","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the effects of super-activated platelet lysate (sPL) on the proliferation, migration, and chondrogenic differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs).</p><p><strong>Methods: </strong>Cell proliferation and migration assay, enzyme-linked immunosorbent analysis, RT-qPCR, chondrogenic differentiation, and Western blot were performed on sPL-treated hUCMSCs.</p><p><strong>Results: </strong>The extraction of sPL from PRP yielded a significant release of growth factors. The proliferation of hUCMSCs was significantly enhanced by both 5% and 10% sPL, and the 5% sPL showed the most promising results. Additionally, 10% sPL demonstrated the strongest positive effect on hUCMSC migration. The 20% sPL group showed a significant decrease in inflammatory factor levels compared to the 0% sPL group when hIL-1β was added to the differentiation system. Notably, hUCMSCs in the 5% sPL, 10% sPL and 20% sPL groups displayed excellent chondrogenic differentiation.</p><p><strong>Conclusion: </strong>In summary, sPL demonstrates the ability to promote stem cell proliferation, migration, and chondrogenic differentiation, while also suppressing inflammation.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":" ","pages":"56-68"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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