Super-activated platelet lysate promotes mesenchymal stem cell proliferation, migration, and chondrogenic differentiation: an in vitro study.

IF 1.8 4区生物学 Q4 CELL BIOLOGY

Growth factors Pub Date : 2025-03-31 DOI:10.1080/08977194.2025.2484614

Chunxiang Liu, Tianqi Zhang, Lingqi Meng, Ihsan Ullah, Heng Li, Fuge Sui, Yi Zhang

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引用次数: 0

Abstract

Objective: This study aimed to investigate the effects of super-activated platelet lysate (sPL) on the proliferation, migration, and chondrogenic differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs).

Methods: Cell proliferation and migration assay, enzyme-linked immunosorbent analysis, RT-qPCR, chondrogenic differentiation, and Western blot were performed on sPL-treated hUCMSCs.

Results: The extraction of sPL from PRP yielded a significant release of growth factors. The proliferation of hUCMSCs was significantly enhanced by both 5% and 10% sPL, and the 5% sPL showed the most promising results. Additionally, 10% sPL demonstrated the strongest positive effect on hUCMSC migration. The 20% sPL group showed a significant decrease in inflammatory factor levels compared to the 0% sPL group when hIL-1β was added to the differentiation system. Notably, hUCMSCs in the 5% sPL, 10% sPL and 20% sPL groups displayed excellent chondrogenic differentiation.

Conclusion: In summary, sPL demonstrates the ability to promote stem cell proliferation, migration, and chondrogenic differentiation, while also suppressing inflammation.

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超活化血小板裂解液促进间充质干细胞增殖、迁移和软骨分化：一项体外研究。

目的：研究超活化血小板裂解液（sPL）对人脐带间充质干细胞（hUCMSCs）增殖、迁移和成软骨分化的影响。方法：采用细胞增殖和迁移实验、酶联免疫吸附分析、RT-qPCR、成软骨分化和Western blot等方法对spll处理的hUCMSCs进行检测。结果：从PRP中提取的sPL有明显的生长因子释放。5%和10%的sPL均能显著促进hUCMSCs的增殖，其中5%的sPL效果最好。此外，10% sPL对hUCMSC迁移的正向影响最强。当向分化系统中添加hIL-1β时，20% sPL组的炎症因子水平明显低于0% sPL组。值得注意的是，5% sPL、10% sPL和20% sPL组的hUCMSCs表现出良好的软骨分化。结论：综上所述，sPL具有促进干细胞增殖、迁移和软骨分化的能力，同时还能抑制炎症。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Growth factors 生物-内分泌学与代谢

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications