genesis最新文献_第5页

ATRX: From Chromatin Remodeling to Disease ATRX：从染色质重塑到疾病

IF 2.4 4区生物学

genesis Pub Date : 2025-11-12 DOI: 10.1002/dvg.70031

Mauro Magaña-Acosta, Viviana Valadez-Graham

引用次数: 0

NOLC1 as Key Regulator of NPR1 (Coding for NPRA) Transcription in Endothelial Senescence 内皮细胞衰老过程中NPR1（编码NPRA）转录的关键调控因子

IF 2.4 4区生物学

genesis Pub Date : 2025-11-06 DOI: 10.1002/dvg.70030

Cui Cui, Wanli Xiao, Jiankun Liu, Hongjin Zeng, Min Wang, You Han, Jiajia Li, Xiaolei Wei, Hongfei Liu, Bing Zhang

{"title":"NOLC1 as Key Regulator of NPR1 (Coding for NPRA) Transcription in Endothelial Senescence","authors":"Cui Cui, Wanli Xiao, Jiankun Liu, Hongjin Zeng, Min Wang, You Han, Jiajia Li, Xiaolei Wei, Hongfei Liu, Bing Zhang","doi":"10.1002/dvg.70030","DOIUrl":"10.1002/dvg.70030","url":null,"abstract":"<div>\u0000 \u0000 <p>Vascular endothelial cell senescence is closely associated with cardiovascular disease. The deficiency of natriuretic peptide receptor A (NPRA), encoded by the natriuretic peptide receptor 1 (<i>NPR1</i>) gene, contributes to vascular endothelial aging, but the cause of its reduced expression is unclear. In this study, we performed reverse chromatin immunoprecipitation (R-ChIP) to analyze the binding proteins of the <i>NPR1</i> promoter and found that nucleolar and coiled body phosphoprotein 1 (<i>NOLC1</i>) functions as a key regulator of NPRA transcription in endothelial cells. Similar to NPRA, NOLC1 is also expressed at reduced levels in senescent endothelial cells. Knockdown of NOLC1 decreased both NPRA mRNA and protein expression levels. Furthermore, inhibition of NOLC1 expression triggered cellular senescence hallmarks, including elevated p53/p21 levels, enhanced SA-β-gal activity, ROS accumulation, G0/G1 cell cycle arrest, and impaired migration. NPRA overexpression rescued senescence and dysfunction in <i>NOLC1</i>-deficient cells, restoring proliferative and migratory capacity. In conclusion, our findings demonstrate that nucleolar phosphoprotein NOLC1 is a key regulator of NPRA transcription in endothelial senescence.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Image, Volume 63, Issue 5 封面图片，第63卷，第5期

IF 2.4 4区生物学

genesis Pub Date : 2025-10-27 DOI: 10.1002/dvg.70029

Marie L. Nydam, Devon Gamble, Tal Gordon, Laurel S. Hiebert, Brenna Hutchings, Assunta Liberti, Jhimli Mondal, Kaylee Moody, Megan Olhasso, Sydney Popsuj, Atsuko Sato, Fatıma Nur Oğul Ünal, Anna Di Gregorio

引用次数: 0

The Second International Symposium on Women in Tunicate Biology 第二届女性被囊生物学国际研讨会

IF 2.4 4区生物学

genesis Pub Date : 2025-10-11 DOI: 10.1002/dvg.70028

Marie L. Nydam, Devon Gamble, Tal Gordon, Laurel S. Hiebert, Brenna Hutchings, Assunta Liberti, Jhimli Mondal, Kaylee Moody, Megan Olhasso, Sydney Popsuj, Atsuko Sato, Fatıma Nur Oğul Ünal, Anna Di Gregorio

引用次数: 0

Generation of Knock-In Syrian Hamsters via Zygote Microinjection Using CRISPR/Cas9 Genome Editing 使用CRISPR/Cas9基因组编辑通过受精卵显微注射产生敲入的叙利亚仓鼠

IF 2.4 4区生物学

genesis Pub Date : 2025-09-18 DOI: 10.1002/dvg.70027

Mayo Shigeta, Ken-ichi Inoue, Naoko Shimada, Alisa Tobe, Takaya Abe, Hiroshi Kiyonari

{"title":"Generation of Knock-In Syrian Hamsters via Zygote Microinjection Using CRISPR/Cas9 Genome Editing","authors":"Mayo Shigeta, Ken-ichi Inoue, Naoko Shimada, Alisa Tobe, Takaya Abe, Hiroshi Kiyonari","doi":"10.1002/dvg.70027","DOIUrl":"10.1002/dvg.70027","url":null,"abstract":"<p>Syrian hamsters (<i>Mesocricetus auratus</i>) have long served as valuable model organisms in diverse research fields such as oncology, immunology, and physiology owing to their unique biological and pathological characteristics. Although embryo manipulation techniques such as embryo collection, pronuclear microinjection, and embryo transfer have been established, gene knock-in (KI) hamsters have not yet been reported. Here, we report the successful generation of gene KI Syrian hamsters by microinjecting CRISPR/Cas9 components and plasmid DNA into pronuclear-stage zygotes. Targeted insertion of a DNA cassette up to 8 kb was achieved at the ROSA26 orthologous locus and other genomic sites. Importantly, we confirmed functional expression of a reporter cassette inserted at the ROSA26 site, providing evidence of transcriptional activity at this locus in Syrian hamsters. Furthermore, we demonstrated that frozen-thawed KI embryos could give rise to live offspring using a simplified freezing and thawing protocol originally developed for mice and rats. These results confirm the feasibility and applicability of advanced genome editing technologies in Syrian hamsters. These technological advancements enable the development of versatile KI models for applications such as gene expression monitoring and conditional mutagenesis, thereby expanding the utility of Syrian hamsters as model organisms, comparable to mice and rats.</p>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145082198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of Astrocyte-Selective Cre Driver Lines With Distinct Onsets of Recombination Activity During Development 在发育过程中具有不同重组活性的星形细胞选择性Cre驱动系的产生

IF 2.4 4区生物学

genesis Pub Date : 2025-09-02 DOI: 10.1002/dvg.70025

Yukina Izumi, Tomoya Nakatani, Harumi Takai, Minoru Kumai, Tsutomu Kamisako, Hiroyoshi Ishizaki, Yuichi Ono

{"title":"Generation of Astrocyte-Selective Cre Driver Lines With Distinct Onsets of Recombination Activity During Development","authors":"Yukina Izumi, Tomoya Nakatani, Harumi Takai, Minoru Kumai, Tsutomu Kamisako, Hiroyoshi Ishizaki, Yuichi Ono","doi":"10.1002/dvg.70025","DOIUrl":"https://doi.org/10.1002/dvg.70025","url":null,"abstract":"<div>\u0000 \u0000 <p>Astrocytes are a major glial cell type, playing multiple roles in the development, function, and pathogenesis of the brain. Accordingly, neuronal–astrocyte communication is an important research area. However, because these cell types share the same developmental origin, selective manipulation of each cell type is needed for precise mechanistic understanding. Here, we generated two new Cre driver lines for selective gene manipulation in astrocytes: Slc7a10-IRES-Cre and Aldh1l1-IRES-Cre. An internal ribosome entry site (IRES)-Cre cassette was knocked-in to the 3′-untranslated region of the solute carrier family 7 member 10 (<i>Slc7a10</i>) or aldehyde dehydrogenase 1 family member L1 (<i>Aldh1l1</i>) locus without disrupting gene function. The Slc7a10-IRES-Cre line underwent highly selective recombination in astrocytes of the brain, apart from choroid plexus epithelial cells. The onset of recombination began after completion of differentiation in the astrocyte lineage. By contrast, the Aldh1l1-IRES-Cre line began recombination during astrocyte differentiation at early postnatal stages. Some leaky expression was observed in the oligodendrocyte lineage, probably due to early onset of Cre expression in an uncommitted glial progenitor state. Together, the combination of the two deleter lines with distinct temporal Cre expression patterns serves as valuable tools to understand the development and function of astrocytes.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144927675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Cxcl15 Cre Recombinase Mouse Model Useful to Study Gland Development in the Uterus 一种用于研究子宫腺体发育的cxcl15cre重组酶小鼠模型

IF 2.4 4区生物学

genesis Pub Date : 2025-09-01 DOI: 10.1002/dvg.70026

Andrew M. Kelleher, Hong Im Kim, Greeshma Sai Bayammagari, Daniel J. Davis, Thomas E. Spencer

{"title":"A Cxcl15 Cre Recombinase Mouse Model Useful to Study Gland Development in the Uterus","authors":"Andrew M. Kelleher, Hong Im Kim, Greeshma Sai Bayammagari, Daniel J. Davis, Thomas E. Spencer","doi":"10.1002/dvg.70026","DOIUrl":"https://doi.org/10.1002/dvg.70026","url":null,"abstract":"<div>\u0000 \u0000 <p>The mammalian uterus contains glands in the endometrium that develop only or primarily after birth. In the mouse, endometrial glands govern post implantation pregnancy establishment via regulation of blastocyst implantation, stromal cell decidualization, and placental development. Here, we describe a new uterine glandular epithelium (GE) specific Cre recombinase mouse line that is useful to study endometrial gland development and function. Utilizing CRISPR-Cas9 genome editing, improved Cre recombinase (iCre) was inserted into the endogenous C-X-C motif chemokine ligand 15 (<i>Cxcl15</i>) gene. <i>Cxcl15</i> mRNA, Cxcl15 protein, and <i>Cxcl15-iCre</i> recombinase activity were specific to the developing GE of the uterus. <i>Cxcl15-iCre</i> mice were crossed with floxed <i>Foxa2</i> mice to conditionally delete Foxa2 specifically in the glands of the neonatal mouse uterus. This conditional deletion of Foxa2 in the developing neonatal uterus resulted in adult mice that lacked Foxa2 in the GE of the uterus, and the adult mice were infertile. The studies described here establish that <i>Cxcl15-iCre</i> mice are a valuable resource to elucidate and explore mechanisms regulating the development and function of glands in the uterus.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144923519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Perspectives on Mesenchymal Stem Cells as a Potential Treatment for Periodontal Diseases and Conditions 间充质干细胞作为牙周病和牙周病潜在治疗方法的研究进展

IF 2.4 4区生物学

genesis Pub Date : 2025-08-21 DOI: 10.1002/dvg.70024

Shuyu Cai, Cong Li, Biaowen Wei, Ziyu Ye, Qin Mao, Wanxiang Ye, Mingdeng Rong, Jincheng Zeng

{"title":"Current Perspectives on Mesenchymal Stem Cells as a Potential Treatment for Periodontal Diseases and Conditions","authors":"Shuyu Cai, Cong Li, Biaowen Wei, Ziyu Ye, Qin Mao, Wanxiang Ye, Mingdeng Rong, Jincheng Zeng","doi":"10.1002/dvg.70024","DOIUrl":"https://doi.org/10.1002/dvg.70024","url":null,"abstract":"<p>Periodontal diseases, including periodontitis and gingivitis, constitute a major global health burden, affecting over 1 billion people worldwide. These conditions typically initiate in adulthood and progress chronically, often exhibiting severe manifestations. The socioeconomic impact is particularly acute in low- and middle-income countries, where limited healthcare access exacerbates disease outcomes. Although conventional treatments provide symptomatic relief, they often fail to achieve complete tissue regeneration due to the complex pathophysiology of periodontal destruction. Mesenchymal stem cells (MSCs) have emerged as a transformative therapeutic strategy, demonstrating unique capabilities for immunomodulation, anti-inflammatory effects, and multipotent differentiation. Preclinical studies have documented MSC-mediated regeneration of periodontal ligaments, alveolar bone, and cementum through paracrine signaling and direct tissue integration. Clinical trials further substantiate their potential to improve key outcomes, including clinical attachment levels and probing depth reduction. However, five critical challenges require resolution for successful translation: (1) cellular source standardization, (2) mechanistic understanding of long-term efficacy, (3) safety and immunological profiling, (4) ethical and economic barriers, and (5) clinical translation barriers. This review systematically evaluates current evidence on MSC-based periodontal regeneration, analyzes these translational challenges, and provides strategic guidance for future research. By integrating fundamental science with clinical perspectives, this work advances the development of reliable MSC therapies for periodontal regeneration.</p>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.70024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression Patterns of sfrp Genes During Regeneration in the Holothurian Eupentacta fraudatrix sfrp基因在假海棠再生过程中的表达模式

IF 2.4 4区生物学

genesis Pub Date : 2025-07-29 DOI: 10.1002/dvg.70023

Konstantin Sadriev

引用次数: 0

Development of a Hepatocyte-Specific Temporal Genetic Mouse Model Using Albumin Promoter-Driven FlpER2 Expression 利用白蛋白启动子驱动的FlpER2表达建立肝细胞特异性时间遗传小鼠模型

IF 2.4 4区生物学

genesis Pub Date : 2025-07-09 DOI: 10.1002/dvg.70022

Bin Wang, Jiale Wang, Yang Liu, Gordon He, Yasmin Jahan-Mihan, Yuxi Wang, Jie Hu, Audrey Li, Faheem Muhammad, Yan Bi, Baoan Ji

{"title":"Development of a Hepatocyte-Specific Temporal Genetic Mouse Model Using Albumin Promoter-Driven FlpER2 Expression","authors":"Bin Wang, Jiale Wang, Yang Liu, Gordon He, Yasmin Jahan-Mihan, Yuxi Wang, Jie Hu, Audrey Li, Faheem Muhammad, Yan Bi, Baoan Ji","doi":"10.1002/dvg.70022","DOIUrl":"https://doi.org/10.1002/dvg.70022","url":null,"abstract":"<div>\u0000 \u0000 <p>Tissue-specific gene manipulation using Cre/loxP or Flp/frt recombination systems is a cornerstone of genetically engineered mouse models. In this study, we aim to develop a novel hepatocyte-specific, tamoxifen-inducible Flp mouse line. BAC (bacterial artificial chromosome)-Alb(albumin)-FlpER2(estrogen receptor ligan binding domain) was developed by inserting IRES-FlpER2 cDNA between the translation stop codon and 3′-UTR of the mouse albumin gene in a bacterial artificial chromosome. Upon tamoxifen induction in mice crossed with reporter lines, western blotting, immunohistochemistry, immunofluorescence staining, and X-gal staining (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside Staining) were used to verify the recombination efficiency and specificity of this mouse model. Recombination was highly efficient and specific in hepatocytes, with no recombination detected in intrahepatic cholangiocytes or other organs in this mouse model. We generated a new tamoxifen-induced hepatocyte-specific mouse model with highly efficient recombination specifically in hepatocytes, and this model can be used to generate tumor model lines.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0