{"title":"Unveiling the impact of DNA methylation machinery: Dnmt1 and Dnmt3a in orchestrating oocyte development and cellular homeostasis","authors":"Fatma Uysal, Gozde Sukur, Nazlican Bozdemir, Ozgur Cinar","doi":"10.1002/dvg.23579","DOIUrl":"10.1002/dvg.23579","url":null,"abstract":"<div>\u0000 \u0000 <p>DNA methylation can be considered the most prominent in controlling the gene expression responsible for the balance between cell proliferation and cell death. In this study, we aimed to analyze the distinct contributions of Dnmt1 and Dnmt3a enzymes in oocyte maturation, survival, autophagy, reactive oxygen species (ROS) production, and compensation capacity of Dnmt3b and Dnmt3l enzymes in mouse oocytes. Following confirming the suppression of Dnmt1or Dnmt3a through siRNA application, the assessment involved immunofluorescence staining for Dnmts, 5mC, p62, and ROS levels. Cell death rates showed a noticeable increase while oocyte maturation rates exhibited significant reduction. Global DNA methylation showed a decline, concomitant with elevated p62 and ROS levels upon Dnmt1 or Dnmt3a knockdown. Remarkably, silencing of Dnmt1 led to an upsurge in Dnmt3a expression, whereas Dnmt3a knockdown triggered an increase in Dnmt1 levels. Furthermore, Dnmt3l expression exhibited a notable decrease after silencing of either Dnmt1 or Dnmt3a, while Dnmt3b levels remained comparable between control and siRNA-treated groups. Collectively, this study underscores the pivotal roles of Dnmt1 and Dnmt3a in orchestrating various facets of oocyte development, encompassing maturation, survival, autophagy, and ROS production. These findings offer valuable insights into the intricate regulatory network governed by DNA methylation machinery within the context of oocyte physiology.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138177657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
genesisPub Date : 2023-11-20DOI: 10.1002/dvg.23574
Christina D. Cota
{"title":"Investigating cellular dynamics in tunicates","authors":"Christina D. Cota","doi":"10.1002/dvg.23574","DOIUrl":"10.1002/dvg.23574","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138177656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
genesisPub Date : 2023-11-16DOI: 10.1002/dvg.23580
Stephanie Keer, Karen M. Neilson, Helene Cousin, Himani D. Majumdar, Dominique Alfandari, Steven L. Klein, Sally A. Moody
{"title":"Bop1 is required to establish precursor domains of craniofacial tissues","authors":"Stephanie Keer, Karen M. Neilson, Helene Cousin, Himani D. Majumdar, Dominique Alfandari, Steven L. Klein, Sally A. Moody","doi":"10.1002/dvg.23580","DOIUrl":"10.1002/dvg.23580","url":null,"abstract":"<div>\u0000 \u0000 <p>Bop1 can promote cell proliferation and is a component of the Pes1-Bop1-WDR12 (PeBoW) complex that regulates ribosomal RNA processing and biogenesis. In embryos, however, <i>bop1</i> mRNA is highly enriched in the neural plate, cranial neural crest and placodes, and potentially may interact with Six1, which also is expressed in these tissues. Recent work demonstrated that during development, Bop1 is required for establishing the size of the tadpole brain, retina and cranial cartilages, as well as controlling neural tissue gene expression levels. Herein, we extend this work by assessing the effects of Bop1 knockdown at neural plate and larval stages. Loss of Bop1 expanded neural plate gene expression domains (<i>sox2</i>, <i>sox11</i>, <i>irx1</i>) and reduced neural crest (<i>foxd3</i>, <i>sox9</i>), placode (<i>six1</i>, <i>sox11</i>, <i>irx1</i>, <i>sox9</i>) and epidermal (<i>dlx5</i>) expression domains. At larval stages, Bop1 knockdown reduced the expression of several otic vesicle genes (<i>six1</i>, <i>pax2</i>, <i>irx1</i>, <i>sox9</i>, <i>dlx5</i>, <i>otx2</i>, <i>tbx1</i>) and branchial arch genes that are required for chondrogenesis (<i>sox9</i>, <i>tbx1</i>, <i>dlx5</i>). The latter was not the result of impaired neural crest migration. Together these observations indicate that Bop1 is a multifunctional protein that in addition to its well-known role in ribosomal biogenesis functions during early development to establish the craniofacial precursor domains.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136399934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
genesisPub Date : 2023-11-15DOI: 10.1002/dvg.23573
Anna Di Gregorio, Annamaria Locascio, Filomena Ristoratore, Antonietta Spagnuolo
{"title":"Women researchers in tunicate biology at the Stazione Zoologica Anton Dohrn in Napoli","authors":"Anna Di Gregorio, Annamaria Locascio, Filomena Ristoratore, Antonietta Spagnuolo","doi":"10.1002/dvg.23573","DOIUrl":"10.1002/dvg.23573","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134650249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
genesisPub Date : 2023-11-09DOI: 10.1002/dvg.23570
Kaoru S. Imai
{"title":"Gene regulatory networks in ascidian embryos","authors":"Kaoru S. Imai","doi":"10.1002/dvg.23570","DOIUrl":"10.1002/dvg.23570","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71523067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
genesisPub Date : 2023-11-09DOI: 10.1002/dvg.23567
Billie J. Swalla
{"title":"Reflections on forty years as a female tunicate researcher","authors":"Billie J. Swalla","doi":"10.1002/dvg.23567","DOIUrl":"10.1002/dvg.23567","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71523077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
genesisPub Date : 2023-11-09DOI: 10.1002/dvg.23568
Camille E. Tessier, Aurore M. M. Dupuy, Thomas Pelé, Philippe P. Juin, Jacqueline A. Lees, Vincent J. Guen
{"title":"EMT and primary ciliogenesis: For better or worse in sickness and in health","authors":"Camille E. Tessier, Aurore M. M. Dupuy, Thomas Pelé, Philippe P. Juin, Jacqueline A. Lees, Vincent J. Guen","doi":"10.1002/dvg.23568","DOIUrl":"10.1002/dvg.23568","url":null,"abstract":"<p>Epithelial-mesenchymal transition (EMT) and primary ciliogenesis are two cell-biological programs that are essential for development of multicellular organisms and whose abnormal regulation results in many diseases (i.e., developmental anomalies and cancers). Emerging studies suggest an intricate interplay between these two processes. Here, we discuss physiological and pathological contexts in which their interconnections promote normal development or disease progression. We describe underlying molecular mechanisms of the interplay and EMT/ciliary signaling axes that influence EMT-related processes (i.e., stemness, motility and invasion). Understanding the molecular and cellular mechanisms of the relationship between EMT and primary ciliogenesis may provide new insights in the etiology of diseases related to EMT and cilia dysfunction.</p>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23568","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72015806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
genesisPub Date : 2023-11-08DOI: 10.1002/dvg.23569
Filomena Ristoratore
{"title":"A journey with ascidians in the pigmentation world","authors":"Filomena Ristoratore","doi":"10.1002/dvg.23569","DOIUrl":"10.1002/dvg.23569","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71487824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}