genesis最新文献 - Book学术

Generation of Plexin-B1 Conditional Knockout Mouse With CRISPR/Cas9 Technology 用CRISPR/Cas9技术培养丛蛋白b1条件敲除小鼠

IF 2.4 4区生物学

genesis Pub Date : 2025-06-25 DOI: 10.1002/dvg.70019

Haofei Ni, Kevin Kelley, Ning Xie, Hongyan Zou, Roland H. Friedel

{"title":"Generation of Plexin-B1 Conditional Knockout Mouse With CRISPR/Cas9 Technology","authors":"Haofei Ni, Kevin Kelley, Ning Xie, Hongyan Zou, Roland H. Friedel","doi":"10.1002/dvg.70019","DOIUrl":"https://doi.org/10.1002/dvg.70019","url":null,"abstract":"<div>\u0000 \u0000 Plexins are axon guidance transmembrane receptors that control cytoskeleton and membrane dynamics in development and adult physiology. As plexins are expressed in multiple cell types in various tissues, floxed alleles that enable conditional deletion are needed to facilitate cell type-specific functional analysis. We report here the generation of a conditional floxed allele of Plexin-B1 (gene symbol Plxnb1) in mouse using CRISPR/Cas9 technology to insert two loxP sites flanking critical exons. Targeting reagents (Cas9 protein, sgRNAs, ssODNs) were delivered into single-cell embryos by electroporation. After screening a total of 128 mouse pups by PCR and Sanger sequencing, two mice were identified carrying both loxP sites in the targeted Plxnb1 locus (success rate ~ 1.6%). The usage of Alt-R modified ssODNs increased targeting frequencies at one loxP site, but not the other. We also tested homology directed repair (HDR) enhancer V2 reagent, but addition of the enhancer reduced the viability of mouse embryos. The Plxnb1flox allele was successfully transmitted through the germline in Mendelian ratios, and effective excision of the floxed region was confirmed by breeding with Cre recombinase strains.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Mechanism That Defines the Characteristic Neurogenesis Patterns in the Neural Plate Using Hes/her Genes During Early Vertebrate Development 在脊椎动物早期发育过程中，利用he /her基因确定神经板中特征性神经发生模式的遗传机制

IF 2.4 4区生物学

genesis Pub Date : 2025-06-02 DOI: 10.1002/dvg.70015

Takero Ohyanagi, Hiroki Tokizaki, Takehisa Sato, Momo Tsuruoka, Kyo Yamasu

{"title":"Genetic Mechanism That Defines the Characteristic Neurogenesis Patterns in the Neural Plate Using Hes/her Genes During Early Vertebrate Development","authors":"Takero Ohyanagi, Hiroki Tokizaki, Takehisa Sato, Momo Tsuruoka, Kyo Yamasu","doi":"10.1002/dvg.70015","DOIUrl":"https://doi.org/10.1002/dvg.70015","url":null,"abstract":"In the early zebrafish neural plate, proneural cluster domains are defined by surrounding neural progenitor pools (NPPs), generating primary neurogenesis patterns. In each NPP, several Notch-independent Hes/her-type genes are expressed in distinct manners. Previous knockdown (KD) experiments induced ectopic neurogenesis in NPPs where only the targeted her genes were expressed, with other her genes absent, suggesting cooperative functions of Notch-independent her genes. In this study, to overcome the inherent limitations in KD approaches, we knocked out (KO) three her genes, her3, her5, and her11, using genome editing techniques. The resulting mutants exhibited ectopic neurogenesis patterns at the end of gastrulation, similar to those observed in KD experiments. KOs of her5 and her11 induced ectopic neurogenesis around the midbrain-hindbrain boundary, whereas her3 KO led to ectopic neurogenesis in rhombomere 1/2 and r4. In these cases, the expression of other Notch-independent her genes was not affected, except for her11, whose expression depended on her5. Analyses of compound mutants revealed that their phenotypes were essentially the sum of those of individual her mutants, indicating independent suppression of neurogenesis by Notch-independent her genes. In conclusion, different Notch-independent her genes collectively define the characteristic pattern of primary neurogenesis in the neural plate.","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.70015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Review of Mitochondrial Complex I During Mammalian Oocyte Maturation 哺乳动物卵母细胞成熟过程中线粒体复合体I的研究综述

IF 2.4 4区生物学

genesis Pub Date : 2025-06-02 DOI: 10.1002/dvg.70017

Nazlican Bozdemir, Ceren Cakir, Ulas Topcu, Fatma Uysal

{"title":"A Comprehensive Review of Mitochondrial Complex I During Mammalian Oocyte Maturation","authors":"Nazlican Bozdemir, Ceren Cakir, Ulas Topcu, Fatma Uysal","doi":"10.1002/dvg.70017","DOIUrl":"https://doi.org/10.1002/dvg.70017","url":null,"abstract":"This review provides a comprehensive overview of Complex I during mammalian oocyte maturation. Complex I (NADH:ubiquinone oxidoreductase) is a crucial member of the electron transport chain and serves two principal functions during oxidative phosphorylation: NADH oxidation and proton pumping. It is located at the inner mitochondrial membrane and consists of 14 core and 31 accessory subunits that are necessary for its function and assembly. Moreover, Complex I is the primary site of reactive oxygen species (ROS) production among the different tissues. In light of the literature, it has been demonstrated that ROS and oxidative stress are significantly important among the various factors that can affect oocyte maturation. Factors such as malnutrition, alcohol use, obesity, PCOS, aging, and smoking are some of the common causes of infertility. Each one of them causes disruption in the equilibrium of the body's redox system and related with oxidative stress. During oocyte maturation, excessive ROS levels are associated with chromosomal errors and developmental insufficiency. In addition, excess oxidative stress adversely affects embryo growth and development and may cause fetal embryopathies with damage to macromolecules in the cytoskeleton. At this particular juncture, Complex I plays a key role in determining ROS production and the success of the oocyte maturation. This review evaluates mitochondrial Complex I's function, structure, and its crucial role during oocyte maturation.","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.70017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zmym2 Alters Expression of Key Craniofacial Genes Zmym2改变颅面关键基因的表达

IF 2.4 4区生物学

genesis Pub Date : 2025-05-30 DOI: 10.1002/dvg.70018

Karyn Jourdeuil, Karen M. Neilson, Andre L. P. Tavares, Sally A. Moody

{"title":"Zmym2 Alters Expression of Key Craniofacial Genes","authors":"Karyn Jourdeuil, Karen M. Neilson, Andre L. P. Tavares, Sally A. Moody","doi":"10.1002/dvg.70018","DOIUrl":"https://doi.org/10.1002/dvg.70018","url":null,"abstract":"<div>\u0000 \u0000 To identify novel Six1-interacting proteins, we previously screened the fly interactome for Sine oculis-binding partners whose orthologues are also expressed in Xenopus embryos. We identified a zinc-finger MYM-containing protein—Zmym2—based on its sequence similarity in a few domains also found in the Drosophila and vertebrate Sine oculis-binding proteins (Sobp). Because recent studies established Zmym2 as a transcriptional repressor that interacts with Six4 during renal development, herein we assessed whether it interacts with Six1, can modify Six1's transcriptional activity, and is involved in cranial neural crest or placode gene expression. Although during early development Zmym2 is expressed in many of the same tissues as Six1 and contains several domains also found in Sobp, we did not detect any interaction by co-immunoprecipitation and did not detect any effect on Six1 + Eya1 transcriptional activity in cultured cells. Nonetheless, increasing the level of Zmym2 in embryos resulted in broader expression domains of neural border, neural tube and neural crest genes, and smaller placode gene domains. These results suggest that although Zmym2 is unlikely to be a bone fide Six1 interacting protein, it appears to indirectly antagonize Six1 function during cranial placode development, promoting neural plate and neural crest gene expression.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Application of scRNA-Seq in Heart Development and Regeneration scRNA-Seq在心脏发育和再生中的应用

IF 2.4 4区生物学

genesis Pub Date : 2025-04-29 DOI: 10.1002/dvg.70013

Junying Gao, Lindong Yu, Haoran Qi, Jun Qi, Zhaodi Zheng

引用次数: 0

Excluding the Genomic Location of Pax2 Regulatory Elements for the Developing Mouse Eye 排除发育中的小鼠眼睛Pax2调控元件的基因组位置

IF 2.4 4区生物学

genesis Pub Date : 2025-04-29 DOI: 10.1002/dvg.70016

Tzu-Hua Ho, Daniela Santamaria-Munoz, Hollin Hamelynck, Anna La Torre, Tom Glaser, Nadean L. Brown

引用次数: 0

Generation of Myeloid-Specific Bmal1 Knockout Mice and Identification of Bmal1-Regulated Ferroptosis in Macrophages 骨髓特异性Bmal1敲除小鼠的产生和巨噬细胞中Bmal1调控的铁凋亡的鉴定

IF 2.4 4区生物学

genesis Pub Date : 2025-04-08 DOI: 10.1002/dvg.70014

Qing Chen, Wenyi Wang, Weijun Fang, Lianhua Qin, Jie Wang, Xiaochen Huang, Sha Pan, Ruijuan Zheng

{"title":"Generation of Myeloid-Specific Bmal1 Knockout Mice and Identification of Bmal1-Regulated Ferroptosis in Macrophages","authors":"Qing Chen, Wenyi Wang, Weijun Fang, Lianhua Qin, Jie Wang, Xiaochen Huang, Sha Pan, Ruijuan Zheng","doi":"10.1002/dvg.70014","DOIUrl":"https://doi.org/10.1002/dvg.70014","url":null,"abstract":"<div>\u0000 \u0000 Circadian clocks have a fundamental role in many physiological processes. Bmal1 (basic helix–loop–helix ARNT like 1) is a central master circadian clock gene. The global Bmal1 knockout mice were shown to have a loss of circadian rhythms, acceleration of aging, and shortened life span. However, global Bmal1 knockout mice did not exactly reflect the Bmal1 function in specific cell or tissue types. To address the importance of circadian rhythms in macrophages, we generated myeloid-specific Bmal1 knockout mice. The efficacy of Bmal1 gene deletion in macrophages was identified at DNA, transcription, protein levels, and function. In contrast to global Bmal1 knockout mice, Bmal1flox/flox and Bmal1mye−/− mice did not exhibit aging phenotypes. However, the deletion of Bmal1 resulted in a loss of rhythmic expression of the circadian genes in macrophages. RNA-Seq revealed that Bmal1 regulated the expression of cell death-related genes in macrophages. Furthermore, these genes have been identified as clock-controlled genes in rhythmic cell models, and Bmal1 controlled the rhythmic expression of these genes in macrophages. Finally, Bmal1 inhibited RSL3-induced ferroptosis in macrophages through Phgdh. In summary, the model of myeloid-specific Bmal1 knockout mice was successfully constructed, providing a tool for the study of the roles of Bmal1 in macrophages and the peripheral circadian clock. Meanwhile, Bmal1 regulates ferroptosis in macrophages.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CACNA1G, A Heterotaxy Candidate Gene, Plays a Role in Ciliogenesis and Left-Right Patterning in Xenopus tropicalis CACNA1G基因在热带爪蟾纤毛发生和左右模式中的作用

IF 2.4 4区生物学

genesis Pub Date : 2025-02-26 DOI: 10.1002/dvg.70009

Valentyna Kostiuk, Rakib Kabir, Rashid Akbari, Amy Rushing, Delfina P. González, Angelina Kim, Ashley Kim, David Zenisek, Mustafa K. Khokha

{"title":"CACNA1G, A Heterotaxy Candidate Gene, Plays a Role in Ciliogenesis and Left-Right Patterning in Xenopus tropicalis","authors":"Valentyna Kostiuk, Rakib Kabir, Rashid Akbari, Amy Rushing, Delfina P. González, Angelina Kim, Ashley Kim, David Zenisek, Mustafa K. Khokha","doi":"10.1002/dvg.70009","DOIUrl":"https://doi.org/10.1002/dvg.70009","url":null,"abstract":"<div>\u0000 \u0000 Heterotaxy (HTX) is characterized by an abnormality in the organ arrangement along the Left-Right (LR) axis and is caused by the disruption of LR patterning in early development. LR asymmetry is critical for multiple organs. Specifically, proper LR patterning is crucial for cardiac function and is a cause of congenital heart disease (CHD). CACNA1G is a candidate gene identified in patients with CHD and HTX. This gene encodes a T-type, low-voltage-activated calcium channel and is a member of the Cav3.1 channel family. However, its function in cardiac or embryonic development remains unknown. Here, we show that abnormal cacna1g expression in Xenopus tropicalis recapitulates the HTX phenotype found in the patient cohort. By examining early LR patterning markers, including pitx2c and dand5, we discovered that both markers are expressed abnormally, suggesting that LR patterning is disrupted at the earliest stages of the LR signaling cascade. Since cilia have been described as key regulators of LR asymmetry, we checked the process of cilia formation in cacna1g crispants. The LR Organizer (LRO) contained reduced cilia quantity in the cacna1g crispants, which may explain the LR defects. In conclusion, the abnormal expression of cacna1g affects cilia in the LRO, leading to abnormal LR patterning and cardiac looping.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meet Our Editorial Board—Genesis. An Interview With Jun (Kelly) Liu, Cornell University, New York, USA 认识我们的编辑委员会--创世纪。美国纽约康奈尔大学刘俊（Kelly）访谈录

IF 2.4 4区生物学

genesis Pub Date : 2025-02-26 DOI: 10.1002/dvg.70006

Jun (Kelly) Liu, Paul Trevorrow

引用次数: 0

Meet Our Editorial Board—Genesis: An Interview With Eric Bellefroid, University Libre de Bruxelles, Bruxelles, Belgium 见见我们的编辑委员会——《创世纪》：采访比利时布鲁塞尔自由大学的埃里克·贝勒弗罗伊德

IF 2.4 4区生物学

genesis Pub Date : 2025-02-26 DOI: 10.1002/dvg.70003

Paul Trevorrow, Eric Bellefroid

引用次数: 0