genesis最新文献 - Book学术

The Application of scRNA-Seq in Heart Development and Regeneration scRNA-Seq在心脏发育和再生中的应用

IF 2.4 4区生物学

genesis Pub Date : 2025-04-29 DOI: 10.1002/dvg.70013

Junying Gao, Lindong Yu, Haoran Qi, Jun Qi, Zhaodi Zheng

引用次数: 0

Excluding the Genomic Location of Pax2 Regulatory Elements for the Developing Mouse Eye 排除发育中的小鼠眼睛Pax2调控元件的基因组位置

IF 2.4 4区生物学

genesis Pub Date : 2025-04-29 DOI: 10.1002/dvg.70016

Tzu-Hua Ho, Daniela Santamaria-Munoz, Hollin Hamelynck, Anna La Torre, Tom Glaser, Nadean L. Brown

引用次数: 0

Generation of Myeloid-Specific Bmal1 Knockout Mice and Identification of Bmal1-Regulated Ferroptosis in Macrophages 骨髓特异性Bmal1敲除小鼠的产生和巨噬细胞中Bmal1调控的铁凋亡的鉴定

IF 2.4 4区生物学

genesis Pub Date : 2025-04-08 DOI: 10.1002/dvg.70014

Qing Chen, Wenyi Wang, Weijun Fang, Lianhua Qin, Jie Wang, Xiaochen Huang, Sha Pan, Ruijuan Zheng

{"title":"Generation of Myeloid-Specific Bmal1 Knockout Mice and Identification of Bmal1-Regulated Ferroptosis in Macrophages","authors":"Qing Chen, Wenyi Wang, Weijun Fang, Lianhua Qin, Jie Wang, Xiaochen Huang, Sha Pan, Ruijuan Zheng","doi":"10.1002/dvg.70014","DOIUrl":"https://doi.org/10.1002/dvg.70014","url":null,"abstract":"<div>\u0000 \u0000 Circadian clocks have a fundamental role in many physiological processes. Bmal1 (basic helix–loop–helix ARNT like 1) is a central master circadian clock gene. The global Bmal1 knockout mice were shown to have a loss of circadian rhythms, acceleration of aging, and shortened life span. However, global Bmal1 knockout mice did not exactly reflect the Bmal1 function in specific cell or tissue types. To address the importance of circadian rhythms in macrophages, we generated myeloid-specific Bmal1 knockout mice. The efficacy of Bmal1 gene deletion in macrophages was identified at DNA, transcription, protein levels, and function. In contrast to global Bmal1 knockout mice, Bmal1flox/flox and Bmal1mye−/− mice did not exhibit aging phenotypes. However, the deletion of Bmal1 resulted in a loss of rhythmic expression of the circadian genes in macrophages. RNA-Seq revealed that Bmal1 regulated the expression of cell death-related genes in macrophages. Furthermore, these genes have been identified as clock-controlled genes in rhythmic cell models, and Bmal1 controlled the rhythmic expression of these genes in macrophages. Finally, Bmal1 inhibited RSL3-induced ferroptosis in macrophages through Phgdh. In summary, the model of myeloid-specific Bmal1 knockout mice was successfully constructed, providing a tool for the study of the roles of Bmal1 in macrophages and the peripheral circadian clock. Meanwhile, Bmal1 regulates ferroptosis in macrophages.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CACNA1G, A Heterotaxy Candidate Gene, Plays a Role in Ciliogenesis and Left-Right Patterning in Xenopus tropicalis CACNA1G基因在热带爪蟾纤毛发生和左右模式中的作用

IF 2.4 4区生物学

genesis Pub Date : 2025-02-26 DOI: 10.1002/dvg.70009

Valentyna Kostiuk, Rakib Kabir, Rashid Akbari, Amy Rushing, Delfina P. González, Angelina Kim, Ashley Kim, David Zenisek, Mustafa K. Khokha

{"title":"CACNA1G, A Heterotaxy Candidate Gene, Plays a Role in Ciliogenesis and Left-Right Patterning in Xenopus tropicalis","authors":"Valentyna Kostiuk, Rakib Kabir, Rashid Akbari, Amy Rushing, Delfina P. González, Angelina Kim, Ashley Kim, David Zenisek, Mustafa K. Khokha","doi":"10.1002/dvg.70009","DOIUrl":"https://doi.org/10.1002/dvg.70009","url":null,"abstract":"<div>\u0000 \u0000 Heterotaxy (HTX) is characterized by an abnormality in the organ arrangement along the Left-Right (LR) axis and is caused by the disruption of LR patterning in early development. LR asymmetry is critical for multiple organs. Specifically, proper LR patterning is crucial for cardiac function and is a cause of congenital heart disease (CHD). CACNA1G is a candidate gene identified in patients with CHD and HTX. This gene encodes a T-type, low-voltage-activated calcium channel and is a member of the Cav3.1 channel family. However, its function in cardiac or embryonic development remains unknown. Here, we show that abnormal cacna1g expression in Xenopus tropicalis recapitulates the HTX phenotype found in the patient cohort. By examining early LR patterning markers, including pitx2c and dand5, we discovered that both markers are expressed abnormally, suggesting that LR patterning is disrupted at the earliest stages of the LR signaling cascade. Since cilia have been described as key regulators of LR asymmetry, we checked the process of cilia formation in cacna1g crispants. The LR Organizer (LRO) contained reduced cilia quantity in the cacna1g crispants, which may explain the LR defects. In conclusion, the abnormal expression of cacna1g affects cilia in the LRO, leading to abnormal LR patterning and cardiac looping.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meet Our Editorial Board—Genesis. An Interview With Jun (Kelly) Liu, Cornell University, New York, USA 认识我们的编辑委员会--创世纪。美国纽约康奈尔大学刘俊（Kelly）访谈录

IF 2.4 4区生物学

genesis Pub Date : 2025-02-26 DOI: 10.1002/dvg.70006

Jun (Kelly) Liu, Paul Trevorrow

引用次数: 0

Meet Our Editorial Board—Genesis: An Interview With Eric Bellefroid, University Libre de Bruxelles, Bruxelles, Belgium 见见我们的编辑委员会——《创世纪》：采访比利时布鲁塞尔自由大学的埃里克·贝勒弗罗伊德

IF 2.4 4区生物学

genesis Pub Date : 2025-02-26 DOI: 10.1002/dvg.70003

Paul Trevorrow, Eric Bellefroid

引用次数: 0

Meet Our Editorial Board—Genesis. An Interview With, Mark Lewandoski, National Cancer Institute, Maryland, USA 见见我们的编辑委员会——创世纪。采访马克·莱万多斯基，美国马里兰州国家癌症研究所

IF 2.4 4区生物学

genesis Pub Date : 2025-02-26 DOI: 10.1002/dvg.70007

Mark Lewandoski, Paul Trevorrow

引用次数: 0

Unraveling the Mechanisms That Regulate Osteoclast Differentiation: A Review of Current Advances 揭示调节破骨细胞分化的机制：综述当前进展

IF 2.4 4区生物学

genesis Pub Date : 2025-02-17 DOI: 10.1002/dvg.70012

Sai Zhang, Meng Gao, Shuzhe Song, Tongdan Zhao, Bianhua Zhou, Hongwei Wang, Weishun Tian, Wenpeng Zhao, Jing Zhao

引用次数: 0

A New Targeted Transgenic Mouse Line for the Study of Protocadherin γC4 研究原钙粘蛋白γ - c4的新靶向转基因小鼠系

IF 2.4 4区生物学

genesis Pub Date : 2025-02-09 DOI: 10.1002/dvg.70010

Cathy M. McLeod, Camille M. Hanes, Leah C. Fuller, Samjhana Bhandari, Hannah G. Lanthier, Robert W. Burgess, Joshua A. Weiner, Andrew M. Garrett

{"title":"A New Targeted Transgenic Mouse Line for the Study of Protocadherin γC4","authors":"Cathy M. McLeod, Camille M. Hanes, Leah C. Fuller, Samjhana Bhandari, Hannah G. Lanthier, Robert W. Burgess, Joshua A. Weiner, Andrew M. Garrett","doi":"10.1002/dvg.70010","DOIUrl":"https://doi.org/10.1002/dvg.70010","url":null,"abstract":"<div>\u0000 \u0000 The γ-protocadherins (γ-Pcdhs) comprise 22 homophilic cell adhesion molecule isoforms, expressed from the Pcdhg gene cluster via promoter choice mechanisms that serve many crucial functions during neural development. Emerging evidence supports the hypothesis that distinct isoforms have unique functions. The γC4 isoform, which is expressed from the Pcdhgc4 promoter and includes its unique variable exon, is the sole γ-Pcdh isoform essential for the postnatal survival in mice. Here we describe a new mouse line (C4-GFP) in which Pcdhgc4 with a C-terminal GFP tag is expressed from the Rosa26 locus following excision of a lox-Stop-lox cassette by Cre recombinase. We report that restricted expression of this transgene in the nervous system using Nestin-Cre is sufficient to rescue the neonatal lethality of mice mutant for Pcdhgc4. This new line will be a vital tool for dissecting mechanisms underlying the functions of this essential cell adhesion molecule gene, mutations in which have been associated with neurodevelopmental disorders in humans.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meet Our Editorial Board—Genesis. An Interview With, Susan Mackem, National Cancer Institute, Maryland, USA 见见我们的编辑委员会——创世纪。采访美国马里兰州国家癌症研究所的苏珊·麦克姆。

IF 2.4 4区生物学

genesis Pub Date : 2025-01-29 DOI: 10.1002/dvg.70011

Paul Trevorrow, Susan Mackem

引用次数: 0