genesis最新文献 - Book学术

The Intersex Gene Is Crucial for Female Fertility and Embryonic Viability in the Red Flour Beetle, Tribolium castaneum 间性基因对红粉甲虫雌性生殖能力和胚胎存活至关重要

IF 2.4 4区生物学

genesis Pub Date : 2026-04-13 DOI: 10.1002/dvg.70051

Chhavi Choudhary, Siddhi Paliwal, Keshav Kumar Meghwanshi, Sundaram Shilpi, Jayendra Nath Shukla

{"title":"The Intersex Gene Is Crucial for Female Fertility and Embryonic Viability in the Red Flour Beetle, Tribolium castaneum","authors":"Chhavi Choudhary, Siddhi Paliwal, Keshav Kumar Meghwanshi, Sundaram Shilpi, Jayendra Nath Shukla","doi":"10.1002/dvg.70051","DOIUrl":"https://doi.org/10.1002/dvg.70051","url":null,"abstract":"<div>\u0000 \u0000 The intersex (ix) plays a pivotal role in the sex determination pathway of Drosophila melanogaster. The Drosophila Ix protein interacts with female-specific Doublesex protein to regulate female sexual differentiation. In this study, we functionally characterized the ix homologue (Tcix) in the red flour beetle, Tribolium castaneum. The Tcix gene comprises of four exons and three introns, and encodes a protein of 166 amino acids that contains a conserved ‘Mediator complex subunit 29’ (Med-29) domain. The pre-mRNA of Tcix splices in a non-sex-specific manner and is expressed across all the developmental stages of T. castaneum. RNA interference-mediated knockdown of Tcix in adult beetles led to a significant reduction in its expression in both sexes. Knockdown males did not show any phenotypic changes, but knockdown females displayed compromised ovarian development, resulting in reduced fecundity and failure of eggs to hatch. Interestingly, the expression of vitellogenin-1 (Vg-1), a key gene involved in oocyte development, was significantly downregulated in these females. Further, parental RNAi of Tcix demonstrated the failure in the hatching of eggs, despite no significant effects on the number of eggs laid. The phylogenetic analysis of Tcix revealed its close clustering with other coleopterans, which supports its evolutionary conservation. Overall, our study establishes the critical role of Tcix in female fertility and embryonic development in T. castaneum.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"64 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147668554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Intersex Gene Is Crucial for Female Fertility and Embryonic Viability in the Red Flour Beetle, Tribolium castaneum 间性基因对红粉甲虫雌性生殖能力和胚胎存活至关重要

IF 2.4 4区生物学

genesis Pub Date : 2026-04-13 DOI: 10.1002/dvg.70051

Chhavi Choudhary, Siddhi Paliwal, Keshav Kumar Meghwanshi, Sundaram Shilpi, Jayendra Nath Shukla

{"title":"The Intersex Gene Is Crucial for Female Fertility and Embryonic Viability in the Red Flour Beetle, Tribolium castaneum","authors":"Chhavi Choudhary, Siddhi Paliwal, Keshav Kumar Meghwanshi, Sundaram Shilpi, Jayendra Nath Shukla","doi":"10.1002/dvg.70051","DOIUrl":"https://doi.org/10.1002/dvg.70051","url":null,"abstract":"<div>\u0000 \u0000 The intersex (ix) plays a pivotal role in the sex determination pathway of Drosophila melanogaster. The Drosophila Ix protein interacts with female-specific Doublesex protein to regulate female sexual differentiation. In this study, we functionally characterized the ix homologue (Tcix) in the red flour beetle, Tribolium castaneum. The Tcix gene comprises of four exons and three introns, and encodes a protein of 166 amino acids that contains a conserved ‘Mediator complex subunit 29’ (Med-29) domain. The pre-mRNA of Tcix splices in a non-sex-specific manner and is expressed across all the developmental stages of T. castaneum. RNA interference-mediated knockdown of Tcix in adult beetles led to a significant reduction in its expression in both sexes. Knockdown males did not show any phenotypic changes, but knockdown females displayed compromised ovarian development, resulting in reduced fecundity and failure of eggs to hatch. Interestingly, the expression of vitellogenin-1 (Vg-1), a key gene involved in oocyte development, was significantly downregulated in these females. Further, parental RNAi of Tcix demonstrated the failure in the hatching of eggs, despite no significant effects on the number of eggs laid. The phylogenetic analysis of Tcix revealed its close clustering with other coleopterans, which supports its evolutionary conservation. Overall, our study establishes the critical role of Tcix in female fertility and embryonic development in T. castaneum.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"64 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147668557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metamorphosis and lncRNAs: A Close Relationship 变态和lncrna：密切的关系

IF 2.4 4区生物学

genesis Pub Date : 2026-04-13 DOI: 10.1002/dvg.70052

H. Herrera-Orozco, H. A. Pérez-Mendoza

引用次数: 0

Metamorphosis and lncRNAs: A Close Relationship 变态和lncrna：密切的关系

IF 2.4 4区生物学

genesis Pub Date : 2026-04-13 DOI: 10.1002/dvg.70052

H. Herrera-Orozco, H. A. Pérez-Mendoza

引用次数: 0

Correction to “Roles of Osteoclasts in Alveolar Bone Remodeling” 对“破骨细胞在牙槽骨重塑中的作用”的修正。

IF 2.4 4区生物学

genesis Pub Date : 2026-03-26 DOI: 10.1002/dvg.70049

引用次数: 0

Generation of a Novel Col8a2P2A-CreERT2 Mouse Line Enables Targeted Genetic Manipulation of Corneal Endothelial Cells and Modeling of Endothelial Decompensation 一种新的Col8a2P2A-CreERT2小鼠系的产生使角膜内皮细胞的靶向遗传操作和内皮代偿的建模成为可能。

IF 2.4 4区生物学

genesis Pub Date : 2026-03-16 DOI: 10.1002/dvg.70047

Yong Yuan, Josiah Holt, Samuel Lorry, Yen-Chiao Wang, Yueh-Chiang Hu, Zhixing Ma, Adam Kaufman, Winston Kao, Diego G. Ogando, Chia-Yang Liu

{"title":"Generation of a Novel Col8a2P2A-CreERT2 Mouse Line Enables Targeted Genetic Manipulation of Corneal Endothelial Cells and Modeling of Endothelial Decompensation","authors":"Yong Yuan, Josiah Holt, Samuel Lorry, Yen-Chiao Wang, Yueh-Chiang Hu, Zhixing Ma, Adam Kaufman, Winston Kao, Diego G. Ogando, Chia-Yang Liu","doi":"10.1002/dvg.70047","DOIUrl":"10.1002/dvg.70047","url":null,"abstract":"The corneal endothelium is a monolayer of specialized cells that maintains stromal deturgescence and transparency, functions essential for vision. Despite its clinical importance, the developmental origins and homeostatic programs of the endothelium remain poorly understood, in part due to the lack of a lineage-specific genetic driver. To overcome this limitation, we generated a Col8a2P2A-CreERT2 knock-in mouse line that enables selective genetic manipulations of corneal endothelial cells. Cre activity was validated with reporter alleles and functional importance was assessed by conditional ablation of Col8a2+ cells in adulthood, with phenotypic outcomes evaluated by histology, immunofluorescence, and in vivo imaging. We found that Col8a2P2A-CreERT2 drives robust and specific recombination in corneal endothelial cells. Functional assays demonstrated that Col8a2+ cells contribute continuously to Descemet's membrane synthesis and are essential for maintaining endothelial integrity. Ablation disrupted endothelial density and barrier function, resulting in phenotypes resembling human endothelial dystrophies, including features of Fuchs' endothelial corneal dystrophy and posterior polymorphous corneal dystrophy. These findings identify Col8a2+ cells as indispensable regulators of endothelial development, homeostasis, and disease pathogenesis. The Col8a2P2A-CreERT2 line provides the first corneal endothelium–specific genetic driver, establishing a platform for mechanistic investigation and therapeutic discovery in endothelial disorders.","PeriodicalId":12718,"journal":{"name":"genesis","volume":"64 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12993115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147469771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Male Keratin 13-Cre Expression Causes Unanticipated Germline Deletion in Notch1 Floxed Mice 雄性角蛋白13-Cre表达导致Notch1 Floxed小鼠意外的种系缺失。

IF 2.4 4区生物学

genesis Pub Date : 2026-03-16 DOI: 10.1002/dvg.70046

Robert J. Danaher, Vanessa Alves Handel, Lei Wang, Octavio A. Gonzalez

{"title":"Male Keratin 13-Cre Expression Causes Unanticipated Germline Deletion in Notch1 Floxed Mice","authors":"Robert J. Danaher, Vanessa Alves Handel, Lei Wang, Octavio A. Gonzalez","doi":"10.1002/dvg.70046","DOIUrl":"10.1002/dvg.70046","url":null,"abstract":"<div>\u0000 \u0000 Notch1 receptor activation plays an important role in epithelial homeostasis and immunoregulation; however, its role in oral mucosal immunity remains unknown. Testing the impact of epithelial Notch1 activation in oral innate responses, including antimicrobial proteins like phospholipase A2 group IIA (PLA2-IIA), requires epithelial-specific Notch1 knockout mice. Creating Balb/c; K13Cre; Notch1Flox/Flox mice was initiated by crossing C57BL/6; K13Cre; Notch1Flox/Flox mice (naturally deficient for PLA2-IIA), expressing Cre recombinase under the control of the Krt13 (keratin 13) promoter (K13Cre) and Notch1Flox/Flox alleles to PLA2-IIA expressing Balb/c mice to generate Balb/c:C57BL/6; K13Cre; Notch1Flox heterozygotes and to ultimately create Balb/c; K13Cre; Notch1Flox/Flox (Notch1 deficient) and Balb/c; Notch1Flox/Flox WT littermate controls. While all first-generation non-K13Cre expressing mice had the expected Notch1Flox heterozygote Notch1 genotype, surprisingly, all first-generation K13Cre expressing mice had an apparent K13Cre; Notch1 homozygote genotype, which is impossible when crossing Notch1 and Notch1Flox/Flox homozygotes. We found that the absence of the floxed Notch1 PCR amplicon resulted from recombination between the two LoxP sites flanking the forward Notch1 PCR primer, and the recombined Notch1 allele was present in the germline of first-generation congenic mice. Finally, we show that this unexpected recombination is avoided by using female K13Cre; Notch1Flox donors and is a cautionary reminder that individual promoters are expressed during spermiogenesis.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"64 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147469743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of BARX1 in Development and Disease BARX1在发育和疾病中的作用。

IF 2.4 4区生物学

genesis Pub Date : 2026-03-16 DOI: 10.1002/dvg.70045

Yiwen Ma, Xiaorui Li, Liping Xiao, Ying Wang, Wei Zheng, Guilian Liang, Tao Sun

引用次数: 0

A Gfrα3 Conditional Knockout Mouse Reveals a Requirement for GFRα3/RET Signaling in Cholecystokinin-Positive Enteroendocrine Cells Gfrα3条件敲除小鼠揭示胆囊收缩素阳性肠内分泌细胞对Gfrα3 /RET信号通路的需求

IF 2.4 4区生物学

genesis Pub Date : 2026-02-16 DOI: 10.1002/dvg.70043

Susan Simanjaya, Masato Kinoshita, Mukhamad Sunardi, Keisuke Ito, Masakazu Shinohara, Takaya Abe, Yuzo Kodama, Toshihiro Uesaka, Hideki Enomoto

{"title":"A Gfrα3 Conditional Knockout Mouse Reveals a Requirement for GFRα3/RET Signaling in Cholecystokinin-Positive Enteroendocrine Cells","authors":"Susan Simanjaya, Masato Kinoshita, Mukhamad Sunardi, Keisuke Ito, Masakazu Shinohara, Takaya Abe, Yuzo Kodama, Toshihiro Uesaka, Hideki Enomoto","doi":"10.1002/dvg.70043","DOIUrl":"10.1002/dvg.70043","url":null,"abstract":"<div>\u0000 \u0000 The receptor tyrosine kinase RET regulates the development of multiple neuronal populations through GFRα co-receptors and their cognate ligands. Recent work has shown that RET is also required for enteroendocrine cell (EEC) function; however, the specific GFRα receptor operating in EECs has remained unclear. Here, we identify GFRα3 as the predominant GFRα receptor expressed in EECs. To determine its cell-autonomous role, we generated a Gfrα3 conditional allele and inactivated the Gfrα3 gene specifically in the intestinal epithelium. These Gfrα3 IEcKO mice exhibited a selective reduction in the number of CCK+ EECs among multiple EEC subtypes. A comparative decrease in CCK+ EECs in Ret IEcKO mice suggests that RET/GFRα3 signaling is required for the development and/or maintenance of this EEC lineage. Although CCK+ EECs can indirectly influence glucose metabolism, Gfrα3 IEcKO mice showed no significant change in glucose tolerance under high-fat diet (HFD) conditions. In contrast, they exhibited modest lipid malabsorption on HFD. Together, these findings demonstrate that GFRα3 is implicated in the structure and function of CCK EECs and highlight the utility of the Gfrα3 conditional allele for dissecting the biology of GFRα3-expressing cells.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"64 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146208214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meet Our Editorial Board—Kin Ming Kwan, Chinese University of Hong Kong, Hong Kong, China 认识一下我们的编委——关建明，香港中文大学，中国香港。

IF 2.4 4区生物学

genesis Pub Date : 2026-02-13 DOI: 10.1002/dvg.70032

Kin Ming Kwan, Paul Trevorrow

引用次数: 0