genesis最新文献_第10页

Resolving the mechanisms underlying epithelial-to-mesenchymal transition of the lateral plate mesoderm 解析侧板中胚层上皮向间质转化的机制

IF 1.5 4区生物学

genesis Pub Date : 2023-07-13 DOI: 10.1002/dvg.23531

Axel H. Newton, Craig A. Smith

引用次数: 0

Generation and characterization of a Dkk4-Cre knock-in mouse line Dkk4-Cre 基因敲入小鼠品系的产生和特征描述

IF 1.5 4区生物学

genesis Pub Date : 2023-07-12 DOI: 10.1002/dvg.23532

Houda Khatif, Hisham Bazzi

{"title":"Generation and characterization of a Dkk4-Cre knock-in mouse line","authors":"Houda Khatif, Hisham Bazzi","doi":"10.1002/dvg.23532","DOIUrl":"10.1002/dvg.23532","url":null,"abstract":"Ectodermal appendages in mammals, such as teeth, mammary glands, sweat glands and hair follicles, are generated during embryogenesis through a series of mesenchymal–epithelial interactions. Canonical Wnt signaling and its inhibitors are implicated in the early steps of ectodermal appendage development and patterning. To study the activation dynamics of the Wnt target and inhibitor Dickkopf4 (Dkk4) in ectodermal appendages, we used CRSIPR/Cas9 to generate a Dkk4-Cre knock-in mouse (Mus musculus) line, where the Cre recombinase cDNA replaces the expression of endogenous Dkk4. Using Cre reporters, the Dkk4-Cre activity was evident at the prospective sites of ectodermal appendages, overlapping with the Dkk4 mRNA expression. Unexpectedly, a predominantly mesenchymal cell population in the embryo posterior also showed Dkk4-Cre activity. Lineage-tracing suggested that these cells are likely derived from a few Dkk4-Cre-expressing cells in the epiblast at early gastrulation. Finally, our analyses of Dkk4-Cre-expressing cells in developing hair follicle epithelial placodes revealed intra- and inter-placodal cellular heterogeneity, supporting emerging data on the positional and transcriptional cellular variability in placodes. Collectively, we propose the new Dkk4-Cre knock-in mouse line as a suitable model to study Wnt and DKK4 inhibitor dynamics in early mouse development and ectodermal appendage morphogenesis.","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23532","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10147566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tunicate Eco-Evo-Devo laboratory in IMS-METU 位于 IMS-METU 的 Tunicate Eco-Evo-Devo 实验室。

IF 2.4 4区生物学

genesis Pub Date : 2023-07-11 DOI: 10.1002/dvg.23536

Arzu Karahan

{"title":"Tunicate Eco-Evo-Devo laboratory in IMS-METU","authors":"Arzu Karahan","doi":"10.1002/dvg.23536","DOIUrl":"10.1002/dvg.23536","url":null,"abstract":"I completed my undergraduate education in Atatürk University, Education Faculty, Biology Department. Then pursued my graduate education at the Biology Department of Mersin University. Both my master's and PhD theses were on the biological and population genetics features of various fish species. My initial encounter with tunicates dates back to my Postdoc at Israel Oceanographic and Limnologic Research Institute (IOLR) in 2011, where I was working on a DNA barcoding project. During that time, the entire institute was actively engaged in research on tunicates, and discussions during lunchtime often revolved around this fascinating group of organisms. Prof. Rinkevich usually only spoke seriously about tunicate biology but 1 day he told me “You know Botryllus schlosseri is riding horse in Black Sea coasts of Turkiye.” I was totally surprised and was trying to understand the meaning of this comment from a scientific perspective. He then showed me the picture of a B. schlosseri colony attached to a seahorse. Following several more Postdoc experiences, I began working as a Principal Investigator at Institute of Marine Sciences, Middle East Technical University (IMS-METU) in 2017. Since then, my team and I have been working on tunicate biodiversity, evolutionary biology, genomics, DNA barcoding, metabarcoding, metabolomics, whole-body regeneration (WBR) and aging related pathways.","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23536","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9826504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel qPCR-based technique for identifying avian sex: An illustration within embryonic craniofacial bone 基于 qPCR 的鸟类性别鉴定新技术：胚胎颅面骨内的说明

IF 1.5 4区生物学

genesis Pub Date : 2023-06-24 DOI: 10.1002/dvg.23530

Claire J. Houchen, Maria Bergman-Gonzalez, Erin E. Bumann

{"title":"A novel qPCR-based technique for identifying avian sex: An illustration within embryonic craniofacial bone","authors":"Claire J. Houchen, Maria Bergman-Gonzalez, Erin E. Bumann","doi":"10.1002/dvg.23530","DOIUrl":"10.1002/dvg.23530","url":null,"abstract":"Sex is a biological variable important to consider in all biomedical experiments. However, doing so in avian embryos can be challenging as sex can be morphologically indistinguishable. Unlike humans, female birds are the heterogametic sex with Z and W sex chromosomes. The female-specific W chromosome has previously been identified in chick using a species-specific polymerase chain reaction (PCR) technique. We developed a novel reverse transcription quantitative PCR (RT-qPCR) technique that amplifies the W chromosome gene histidine triad nucleotide-binding protein W (HINTW) in chick, quail, and duck. Accuracy of the HINTW RT-qPCR primer set was confirmed in all three species using species-specific PCR, including a novel quail-specific HINTW PCR primer set. Bone development-related gene expression was then analyzed by sex in embryonic lower jaws of duck and quail, as adult duck beak size is known to be sexually dimorphic while quail beak size is not. Trends toward sex differences were found in duck gene expression but not in quail, as expected. With these novel RT-qPCR and PCR embryo sexing methods, sex of chick, quail, and duck embryos can now be assessed by either/both RNA and DNA, which facilitates analysis of sex as a biological variable in studies using these model organisms.","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23530","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10053454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epithelial–mesenchymal plasticity in kidney fibrosis 肾脏纤维化中的上皮-间质可塑性

IF 1.5 4区生物学

genesis Pub Date : 2023-06-22 DOI: 10.1002/dvg.23529

Sudarat Hadpech, Visith Thongboonkerd

{"title":"Epithelial–mesenchymal plasticity in kidney fibrosis","authors":"Sudarat Hadpech, Visith Thongboonkerd","doi":"10.1002/dvg.23529","DOIUrl":"10.1002/dvg.23529","url":null,"abstract":"<div>\u0000 \u0000 Epithelial–mesenchymal transition (EMT) is an important biological process contributing to kidney fibrosis and chronic kidney disease. This process is characterized by decreased epithelial phenotypes/markers and increased mesenchymal phenotypes/markers. Tubular epithelial cells (TECs) are commonly susceptible to EMT by various stimuli, for example, transforming growth factor-β (TGF-β), cellular communication network factor 2, angiotensin-II, fibroblast growth factor-2, oncostatin M, matrix metalloproteinase-2, tissue plasminogen activator (t-PA), plasmin, interleukin-1β, and reactive oxygen species. Similarly, glomerular podocytes can undergo EMT via these stimuli and by high glucose condition in diabetic kidney disease. EMT of TECs and podocytes leads to tubulointerstitial fibrosis and glomerulosclerosis, respectively. Signaling pathways involved in EMT-mediated kidney fibrosis are diverse and complex. TGF-β1/Smad and Wnt/β-catenin pathways are the major venues triggering EMT in TECs and podocytes. These two pathways thus serve as the major therapeutic targets against EMT-mediated kidney fibrosis. To date, a number of EMT inhibitors have been identified and characterized. As expected, the majority of these EMT inhibitors affect TGF-β1/Smad and Wnt/β-catenin pathways. In addition to kidney fibrosis, these EMT-targeted antifibrotic inhibitors are expected to be effective for treatment against fibrosis in other organs/tissues.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9671422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding EMC foldopathies: Topogenesis deficits alter the neural crest 扩展EMC折叠病：形态发生缺陷改变神经嵴。

IF 1.5 4区生物学

genesis Pub Date : 2023-06-15 DOI: 10.1002/dvg.23520

Jonathan Marquez, Faiza Aslam, Mustafa K. Khokha

{"title":"Expanding EMC foldopathies: Topogenesis deficits alter the neural crest","authors":"Jonathan Marquez, Faiza Aslam, Mustafa K. Khokha","doi":"10.1002/dvg.23520","DOIUrl":"10.1002/dvg.23520","url":null,"abstract":"<div>\u0000 \u0000 The endoplasmic reticulum (ER) membrane protein complex (EMC) is essential for the insertion of a wide variety of transmembrane proteins into the plasma membrane across cell types. Each EMC is composed of Emc1-7, Emc10, and either Emc8 or Emc9. Recent human genetics studies have implicated variants in EMC genes as the basis for a group of human congenital diseases. The patient phenotypes are varied but appear to affect a subset of tissues more prominently than others. Namely, craniofacial development seems to be commonly affected. We previously developed an array of assays in Xenopus tropicalis to assess the effects of emc1 depletion on the neural crest, craniofacial cartilage, and neuromuscular function. We sought to extend this approach to additional EMC components identified in patients with congenital malformations. Through this approach, we determine that EMC9 and EMC10 are important for neural crest development and the development of craniofacial structures. The phenotypes observed in patients and our Xenopus model phenotypes similar to EMC1 loss of function likely due to a similar mechanism of dysfunction in transmembrane protein topogenesis.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23520","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10663659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meeting report: Third Franco-Japanese developmental biology meeting “New Frontiers in developmental biology: Celebrating the diversity of life” 会议报告：第三次法日发育生物学会议“发育生物学的新前沿：庆祝生命的多样性”。

IF 1.5 4区生物学

genesis Pub Date : 2023-06-14 DOI: 10.1002/dvg.23527

Oginuma Masayuki, Anne-Cécile Reymann

{"title":"Meeting report: Third Franco-Japanese developmental biology meeting “New Frontiers in developmental biology: Celebrating the diversity of life”","authors":"Oginuma Masayuki, Anne-Cécile Reymann","doi":"10.1002/dvg.23527","DOIUrl":"10.1002/dvg.23527","url":null,"abstract":"The French and Japanese Developmental Biology Societies, teaming up with Human Frontier Science Program, were eager to meet back in person in November 2022 in the lovely city of Strasbourg. Top scientists in the developmental biology field from France and Japan, but also from United States, United Kingdom, Switzerland or Germany shared their exciting science during the 4 days of this meeting. Core fields of developmental biology such as morphogenesis, patterning, cell identity, and cell state transition, notably at the single cell level, were well represented, and a diversity of experimental models, including plants, animals, and other exotic organisms, as well as some in vitro cellular models, were covered. This event also extended the scope of classic scientific gatherings for two reasons. First the involvement of artists during the preparation of the event and on site. Second, part of the meeting was open for the general public through a series of outreach events, including a music and video presentation through projection mapping at Rohan palace, as well as public lectures.","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23527","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10351434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatio-temporal control of targeted gene expression in combination with CRISPR/Cas and Tet-On systems in Medaka 结合 CRISPR/Cas 和 Tet-On 系统对青鳉体内靶向基因表达进行时空控制

IF 1.5 4区生物学

genesis Pub Date : 2023-05-25 DOI: 10.1002/dvg.23519

Daichi Kayo, Sayaka Kimura, Touko Yamazaki, Kiyoshi Naruse, Hideaki Takeuchi, Satoshi Ansai

引用次数: 0

Histone acetyltransferases and histone deacetyl transferases play crucial role during oogenesis and early embryo development 组蛋白乙酰转移酶和组蛋白脱乙酰转移酶在卵子发生和胚胎早期发育过程中起着至关重要的作用。

IF 1.5 4区生物学

genesis Pub Date : 2023-05-25 DOI: 10.1002/dvg.23518

Nazlican Bozdemir, Fatma Uysal

{"title":"Histone acetyltransferases and histone deacetyl transferases play crucial role during oogenesis and early embryo development","authors":"Nazlican Bozdemir, Fatma Uysal","doi":"10.1002/dvg.23518","DOIUrl":"10.1002/dvg.23518","url":null,"abstract":"<div>\u0000 \u0000 Dynamic epigenetic regulation is critical for proper oogenesis and early embryo development. During oogenesis, fully grown germinal vesicle oocytes develop to mature Metaphase II oocytes which are ready for fertilization. Fertilized oocyte proliferates mitotically until blastocyst formation and the process is called early embryo development. Throughout oogenesis and early embryo development, spatio-temporal gene expression takes place, and this dynamic gene expression is controlled with the aid of epigenetics. Epigenetic means that gene expression can be altered without changing DNA itself. Epigenome is regulated through DNA methylation and histone modifications. While DNA methylation generally ends up with repression of gene expression, histone modifications can result in expression or repression depending on type of modification, type of histone protein and its specific residue. One of the modifications is histone acetylation which generally ends up with gene expression. Histone acetylation occurs through the addition of acetyl group onto amino terminal of the core histone proteins by histone acetyltransferases (HATs). Contrarily, histone deacetylation is associated with repression of gene expression, and it is catalyzed by histone deacetylases (HDACs). This review article focuses on what is known about alterations in the expression of HATs and HDACs and emphasizes importance of HATs and HDACs during oogenesis and early embryo development.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10298385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Cementum is key to periodontal tissue regeneration: A review on apatite microstructures for creation of novel cementum-based dental implants 牙骨质是牙周组织再生的关键：新型牙骨质基牙种植体的磷灰石微观结构综述。

IF 1.5 4区生物学

genesis Pub Date : 2023-04-17 DOI: 10.1002/dvg.23514

Mari M. Saito, Kazuo Onuma, Yasuo Yamakoshi

引用次数: 2