genesis最新文献_第2页

Meet Our Editorial Board—Genesis. An Interview With Yoh-suke Mukouyama, National Institutes of Health, Maryland, USA

IF 2.4 4区生物学

genesis Pub Date : 2025-01-24 DOI: 10.1002/dvg.70008

Yoh-suke Mukouyama, Paul Trevorrow

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Meet Our Editorial Board—Genesis. An Interview With, Sally Moody, The George Washington University School of Medicine and Health Sciences, USA 认识我们的编辑委员会--《创世纪》。专访美国乔治-华盛顿大学医学与健康科学学院莎莉-穆迪。

IF 2.4 4区生物学

genesis Pub Date : 2024-12-31 DOI: 10.1002/dvg.70000

Paul Trevorrow, Sally A. Moody

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Meet Our Editorial Board—Genesis. An Interview With Margot L. K. Williams, Baylor College of Medicine, Texas, USA 见见我们的编辑委员会——创世纪。采访美国德克萨斯州贝勒医学院的玛格特·l·k·威廉姆斯。

IF 2.4 4区生物学

genesis Pub Date : 2024-12-17 DOI: 10.1002/dvg.70001

Paul Trevorrow, Margot L. K. Williams

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Generation and Characterization of a TRIM21 Overexpressing Mouse Line TRIM21过表达小鼠品系的产生和特征描述

IF 2.4 4区生物学

genesis Pub Date : 2024-11-01 DOI: 10.1002/dvg.23616

Lisa M. Mehlmann, Tracy F. Uliasz, Siu-Pok Yee, Deborah Kaback, Katie M. Lowther

{"title":"Generation and Characterization of a TRIM21 Overexpressing Mouse Line","authors":"Lisa M. Mehlmann, Tracy F. Uliasz, Siu-Pok Yee, Deborah Kaback, Katie M. Lowther","doi":"10.1002/dvg.23616","DOIUrl":"10.1002/dvg.23616","url":null,"abstract":"Specific removal of a protein is a key to understanding its function. “Trim-Away” utilizes TRIM21, an antibody receptor and ubiquitin ligase, for acute and specific reduction of proteins. When TRIM21 is expressed in cells, introduction of a specific antibody causes rapid degradation of the targeted protein; however, TRIM21 is endogenously expressed in few cell types. We have generated a mouse line using CRISPR to insert a conditional overexpression cassette of TRIM21 into the safe harbor site, Rosa26. These conditionally-expressing mice can be bred to a wide variety of Cre mice to target cell-specific TRIM21 overexpression in different tissues. Zp3Cre mice expressed TRIM21 protein specifically in oocytes, whereas HprtCre mice expressed the protein globally. When TRIM21-overexpressing oocytes were microinjected with specific antibodies targeting either the IP3 receptor or SNAP23, these proteins were effectively degraded. In addition, cortical neural cells from globally-overexpressing TRIM21 mice showed a dramatic reduction in IP3 receptor protein within hours after electroporation of a specific antibody. These experiments confirm the effectiveness of the Trim-Away method for protein reduction. These mice should make a valuable addition to the broader research community, as a wide range of proteins and cell types can be studied using this method.","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23616","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142565116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and Transcriptional Targets of TGFβ-RII in Paracentrotus lividus Larval Skeletogenesis TGFβ-RII 在红腹角雉幼体骨骼发生过程中的表达和转录靶标

IF 2.4 4区生物学

genesis Pub Date : 2024-08-14 DOI: 10.1002/dvg.23614

Daniel Goloe, Tsvia Gildor, Smadar Ben-Tabou de-Leon

{"title":"Expression and Transcriptional Targets of TGFβ-RII in Paracentrotus lividus Larval Skeletogenesis","authors":"Daniel Goloe, Tsvia Gildor, Smadar Ben-Tabou de-Leon","doi":"10.1002/dvg.23614","DOIUrl":"10.1002/dvg.23614","url":null,"abstract":"Organisms from the five kingdoms of life use minerals to harden their tissues and make teeth, shells and skeletons, in the process of biomineralization. The sea urchin larval skeleton is an excellent system to study the biological regulation of biomineralization and its evolution. The gene regulatory network (GRN) that controls sea urchin skeletogenesis is known in great details and shows similarity to the GRN that controls vertebrates' vascularization while it is quite distinct from the GRN that drives vertebrates' bone formation. Yet, transforming growth factor beta (TGF-β) signaling regulates both sea urchin and vertebrates' skeletogenesis. Here, we study the upstream regulation and identify transcriptional targets of TGF-β in the Mediterranean Sea urchin species, Paracentrotus lividus. TGF-βRII is transiently active in the skeletogenic cells downstream of vascular endothelial growth factor (VEGF) signaling, in P. lividus. Continuous perturbation of TGF-βRII activity significantly impairs skeletal elongation and the expression of key skeletogenic genes. Perturbation of TGF-βRII after skeletal initiation leads to a delay in skeletal elongation and minor changes in gene expression. TGF-β targets are distinct from its transcriptional targets during vertebrates' bone formation, suggesting that the role of TGF-β in biomineralization in these two phyla results from convergent evolution.","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23614","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141977034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of an Armcx1 Conditional Knockout Mouse Armcx1 条件性基因敲除小鼠的产生

IF 2.4 4区生物学

genesis Pub Date : 2024-08-14 DOI: 10.1002/dvg.23615

Cora L. Bright, Howard M. Bomze, Mantu Bhaumik, Jeremy N. Kay, Romain Cartoni, Sidney M. Gospe III

{"title":"Generation of an Armcx1 Conditional Knockout Mouse","authors":"Cora L. Bright, Howard M. Bomze, Mantu Bhaumik, Jeremy N. Kay, Romain Cartoni, Sidney M. Gospe III","doi":"10.1002/dvg.23615","DOIUrl":"10.1002/dvg.23615","url":null,"abstract":"<div>\u0000 \u0000 Armadillo repeat-containing X-linked protein-1 (Armcx1) is a poorly characterized transmembrane protein that regulates mitochondrial transport in neurons. Its overexpression has been shown to induce neurite outgrowth in embryonic neurons and to promote retinal ganglion cell (RGC) survival and axonal regrowth in a mouse optic nerve crush model. In order to evaluate the functions of endogenous Armcx1 in vivo, we have created a conditional Armcx1 knockout mouse line in which the entire coding region of the Armcx1 gene is flanked by loxP sites. This Armcx1fl line was crossed with mouse strains in which Cre recombinase expression is driven by the promoters for β-actin and Six3, in order to achieve deletion of Armcx1 globally and in retinal neurons, respectively. Having confirmed deletion of the gene, we proceeded to characterize the abundance and morphology of RGCs in Armcx1 knockout mice aged to 15 months. Under normal physiological conditions, no evidence of aberrant retinal or optic nerve development or RGC degeneration was observed in these mice. The Armcx1fl mouse should be valuable for future studies investigating mitochondrial morphology and transport in the absence of Armcx1 and in determining the susceptibility of Armcx1-deficient neurons to degeneration in the setting of additional heritable or environmental stressors.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141977035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sox21 homeologs autoregulate expression levels to control progression through neurogenesis Sox21同源物通过自动调节表达水平来控制神经发生的进展。

IF 2.4 4区生物学

genesis Pub Date : 2024-07-26 DOI: 10.1002/dvg.23612

Dillon L. Damuth, Doreen D. Cunningham, Elena M. Silva

{"title":"Sox21 homeologs autoregulate expression levels to control progression through neurogenesis","authors":"Dillon L. Damuth, Doreen D. Cunningham, Elena M. Silva","doi":"10.1002/dvg.23612","DOIUrl":"10.1002/dvg.23612","url":null,"abstract":"<div>\u0000 \u0000 The SRY HMG box transcription factor Sox21 plays multiple critical roles in neurogenesis, with its function dependent on concentration and developmental stage. In the allotetraploid Xenopus laevis, there are two homeologs of sox21, namely sox21.S and sox21.L. Previous studies focused on Sox21.S, but its amino acid sequence is divergent, lacking conserved poly-A stretches and bearing more similarity with ancestral homologs. In contrast, Sox21.L shares higher sequence similarity with mouse and chick Sox21. To determine if Sox21.S and Sox21.L have distinct functions, we conducted gain and loss-of-function studies in Xenopus embryos. Our studies revealed that Sox21.S and Sox21.L are functionally redundant, but Sox21.L is more effective at driving changes than Sox21.S. These results also support our earlier findings in ectodermal explants, demonstrating that Sox21 function is dose-dependent. While Sox21 is necessary for primary neuron formation, high levels prevent their formation. Strikingly, these proteins autoregulate, with high levels of Sox21.L reducing sox21.S and sox21.L mRNA levels, and decreased Sox21.S promoting increased expression of sox21.L. Our findings shed light on the intricate concentration-dependent roles of Sox21 homeologs in Xenopus neurogenesis.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141761971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two decades on: Special issue on olfaction celebrating Axel and Buck's Nobel Prize 二十年后：庆祝阿克塞尔和巴克获得诺贝尔奖的嗅觉特刊。

IF 2.4 4区生物学

genesis Pub Date : 2024-07-26 DOI: 10.1002/dvg.23613

Paolo E. Forni, C. Ron Yu

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The influence of olfactory experience on the birthrates of olfactory sensory neurons with specific odorant receptor identities 嗅觉经验对具有特定气味受体特征的嗅觉神经元出生率的影响

IF 1.5 4区生物学

genesis Pub Date : 2024-06-18 DOI: 10.1002/dvg.23611

Karlin E. Rufenacht, Alexa J. Asson, Kawsar Hossain, Stephen W. Santoro

{"title":"The influence of olfactory experience on the birthrates of olfactory sensory neurons with specific odorant receptor identities","authors":"Karlin E. Rufenacht, Alexa J. Asson, Kawsar Hossain, Stephen W. Santoro","doi":"10.1002/dvg.23611","DOIUrl":"10.1002/dvg.23611","url":null,"abstract":"<div>\u0000 \u0000 Olfactory sensory neurons (OSNs) are one of a few neuron types that are generated continuously throughout life in mammals. The persistence of olfactory sensory neurogenesis beyond early development has long been thought to function simply to replace neurons that are lost or damaged through exposure to environmental insults. The possibility that olfactory sensory neurogenesis may also serve an adaptive function has received relatively little consideration, largely due to the assumption that the generation of new OSNs is stochastic with respect to OSN subtype, as defined by the single odorant receptor gene that each neural precursor stochastically chooses for expression out of hundreds of possibilities. Accordingly, the relative birthrates of different OSN subtypes are predicted to be constant and impervious to olfactory experience. This assumption has been called into question, however, by evidence that the birthrates of specific OSN subtypes can be selectively altered by manipulating olfactory experience through olfactory deprivation, enrichment, and conditioning paradigms. Moreover, studies of recovery of the OSN population following injury provide further evidence that olfactory sensory neurogenesis may not be strictly stochastic with respect to subtype. Here we review this evidence and consider mechanistic and functional implications of the prospect that specific olfactory experiences can regulate olfactory sensory neurogenesis rates in a subtype-selective manner.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141421537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of odorant receptors during olfactory glomerular map formation 嗅觉肾小球图形成过程中气味受体的作用

IF 1.5 4区生物学

genesis Pub Date : 2024-06-14 DOI: 10.1002/dvg.23610

Ai Nakashima, Haruki Takeuchi

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