genesis最新文献_第2页

RSG1 is required for cilia-dependent neural tube closure 纤毛依赖性神经管闭合需要 RSG1

IF 1.5 4区生物学

genesis Pub Date : 2024-05-09 DOI: 10.1002/dvg.23602

David Engelhardt, Amber Marean, David McKean, Juliette Petersen, Lee Niswander

引用次数: 0

A knock-in allele of Hand2 expressing Dre recombinase 表达 Dre 重组酶的 Hand2 基因敲入等位基因

IF 1.5 4区生物学

genesis Pub Date : 2024-05-04 DOI: 10.1002/dvg.23601

Nicholas W. Plummer, Kathleen G. Smith, Patricia Jensen

{"title":"A knock-in allele of Hand2 expressing Dre recombinase","authors":"Nicholas W. Plummer, Kathleen G. Smith, Patricia Jensen","doi":"10.1002/dvg.23601","DOIUrl":"https://doi.org/10.1002/dvg.23601","url":null,"abstract":"HAND2 is a basic helix–loop–helix transcription factor with diverse functions during development. To facilitate the investigation of genetic and functional diversity among Hand2-expressing cells in the mouse, we have generated Hand2Dre, a knock-in allele expressing Dre recombinase. To avoid disrupting Hand2 function, the Dre cDNA is inserted at the 3′ end of the Hand2 coding sequence following a viral 2A peptide. Hand2Dre homozygotes can therefore be used in complex crosses to increase the proportion of useful genotypes among offspring. Dre expression in mid-gestation Hand2Dre embryos is indistinguishable from wild-type Hand2 expression, and HandDre efficiently recombines rox target sites in vivo. In combination with existing Cre and Flp mouse lines, Hand2Dre will therefore extend the ability to perform genetic intersectional labeling, fate mapping, and functional manipulation of subpopulations of cells characterized by developmental expression of Hand2.","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23601","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Following the p63/Keratin5 basal cells in the sensory and non-sensory epithelia of the vomeronasal organ 绒毛器官感觉上皮和非感觉上皮中的 p63/Keratin5 基底细胞

IF 1.5 4区生物学

genesis Pub Date : 2024-04-26 DOI: 10.1002/dvg.23596

Noah M. LeFever, Raghu Ram Katreddi, Nikki M. Dolphin, Nick A. Mathias, Paolo E. Forni

{"title":"Following the p63/Keratin5 basal cells in the sensory and non-sensory epithelia of the vomeronasal organ","authors":"Noah M. LeFever, Raghu Ram Katreddi, Nikki M. Dolphin, Nick A. Mathias, Paolo E. Forni","doi":"10.1002/dvg.23596","DOIUrl":"https://doi.org/10.1002/dvg.23596","url":null,"abstract":"<div>\u0000 \u0000 The vomeronasal organ (VNO) is a part of the accessory olfactory system, which detects pheromones and chemical factors that trigger a spectrum of sexual and social behaviors. The vomeronasal epithelium (VNE) shares several features with the epithelium of the main olfactory epithelium (MOE). However, it is a distinct neuroepithelium populated by chemosensory neurons that differ from the olfactory sensory neurons in cellular structure, receptor expression, and connectivity. The vomeronasal organ of rodents comprises a sensory epithelium (SE) and a thin non-sensory epithelium (NSE) that morphologically resembles the respiratory epithelium. Sox2-positive cells have been previously identified as the stem cell population that gives rise to neuronal progenitors in MOE and VNE. In addition, the MOE also comprises p63 positive horizontal basal cells, a second pool of quiescent stem cells that become active in response to injury. Immunolabeling against the transcription factor p63, Keratin-5 (Krt5), Krt14, NrCAM, and Krt5Cre tracing experiments highlighted the existence of horizontal basal cells distributed along the basal lamina of SE of the VNO. Single cell sequencing and genetic lineage tracing suggest that the vomeronasal horizontal basal cells arise from basal progenitors at the boundary between the SE and NSE proximal to the marginal zones. Moreover, our experiments revealed that the NSE of rodents is, like the respiratory epithelium, a stratified epithelium where the p63/Krt5+ basal progenitor cells self-replicate and give rise to the apical columnar cells facing the lumen of the VNO.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140648199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ectopic expression in commonly used transgenic Drosophila GAL4 driver lines 常用转基因果蝇 GAL4 驱动系的异位表达

IF 1.5 4区生物学

genesis Pub Date : 2024-04-26 DOI: 10.1002/dvg.23600

Mattias Winant, Kurt Buhler, Patrick Callaerts

引用次数: 0

Insertion of a neomycin selection cassette in the Amigo1 locus alters gene expression in the olfactory epithelium leading to region-specific defects in olfactory receptor neuron development 在Amigo1基因座上插入新霉素选择盒会改变嗅上皮细胞的基因表达，导致嗅觉受体神经元发育的区域特异性缺陷

IF 1.5 4区生物学

genesis Pub Date : 2024-04-08 DOI: 10.1002/dvg.23594

Reesha Raja, Emilie Dumontier, Alina Phen, Jean-François Cloutier

{"title":"Insertion of a neomycin selection cassette in the Amigo1 locus alters gene expression in the olfactory epithelium leading to region-specific defects in olfactory receptor neuron development","authors":"Reesha Raja, Emilie Dumontier, Alina Phen, Jean-François Cloutier","doi":"10.1002/dvg.23594","DOIUrl":"https://doi.org/10.1002/dvg.23594","url":null,"abstract":"During development of the nervous system, neurons connect to one another in a precisely organized manner. Sensory systems provide a good example of this organization, whereby the composition of the outside world is represented in the brain by neuronal maps. Establishing correct patterns of neural circuitry is crucial, as inaccurate map formation can lead to severe disruptions in sensory processing. In rodents, olfactory stimuli modulate a wide variety of behaviors essential for survival. The formation of the olfactory glomerular map is dependent on molecular cues that guide olfactory receptor neuron axons to broad regions of the olfactory bulb and on cell adhesion molecules that promote axonal sorting into specific synaptic units in this structure. Here, we demonstrate that the cell adhesion molecule Amigo1 is expressed in a subpopulation of olfactory receptor neurons, and we investigate its role in the precise targeting of olfactory receptor neuron axons to the olfactory bulb using a genetic loss-of-function approach in mice. While ablation of Amigo1 did not lead to alterations in olfactory sensory neuron axonal targeting, our experiments revealed that the presence of a neomycin resistance selection cassette in the Amigo1 locus can lead to off-target effects that are not due to loss of Amigo1 expression, including unexpected altered gene expression in olfactory receptor neurons and reduced glomerular size in the ventral region of the olfactory bulb. Our results demonstrate that insertion of a neomycin selection cassette into the mouse genome can have specific deleterious effects on the development of the olfactory system and highlight the importance of removing antibiotic resistance cassettes from genetic loss-of-function mouse models when studying olfactory system development.","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23594","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140537942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Type 2 vomeronasal receptor-A4 subfamily: Potential predator sensors in mice 2 型绒毛受体-A4 亚家族：小鼠潜在的捕食者传感器

IF 1.5 4区生物学

genesis Pub Date : 2024-04-08 DOI: 10.1002/dvg.23597

Andrea Rocha, Quynh Anh Thi Nguyen, Sachiko Haga-Yamanaka

引用次数: 0

The biological characteristics of chicken embryo mesenchymal stem cells isolated from chorioallantoic membrane 从绒毛膜分离出的鸡胚间充质干细胞的生物学特性

IF 1.5 4区生物学

genesis Pub Date : 2024-04-08 DOI: 10.1002/dvg.23592

Yue Wu, Yunan Wang, Weijun Gan, Wei Jiang

{"title":"The biological characteristics of chicken embryo mesenchymal stem cells isolated from chorioallantoic membrane","authors":"Yue Wu, Yunan Wang, Weijun Gan, Wei Jiang","doi":"10.1002/dvg.23592","DOIUrl":"https://doi.org/10.1002/dvg.23592","url":null,"abstract":"<div>\u0000 \u0000 Mesenchymal stem cells (MSCs) derived from fetal membranes (FMs) have the potential to exhibit immunosuppression, improve blood flow, and increase capillary density during transplantation. In the field of medicine, opening up new avenues for disease treatment. Chicken embryo chorioallantoic membrane (CAM), as an important component of avian species FM structure, has become a stable tissue engineering material in vivo angiogenesis, drug delivery, and toxicology studies. Although it has been confirmed that chorionic mesenchymal stem cells (Ch-MSCs) can be isolated from the outer chorionic layer of FM, little is known about the biological characteristics of MSCs derived from chorionic mesodermal matrix of chicken embryos. Therefore, we evaluated the characteristics of MSCs isolated from chorionic tissues of chicken embryos, including cell proliferation ability, stem cell surface antigen, genetic stability, and in vitro differentiation potential. Ch-MSCs exhibited a broad spindle shaped appearance and could stably maintain diploid karyotype proliferation to passage 15 in vitro. Spindle cells were positive for multifunctional markers of MSCs (CD29, CD44, CD73, CD90, CD105, CD166, OCT4, and NANOG), while hematopoietic cell surface marker CD34, panleukocyte marker CD45, and epithelial cell marker CK19 were negative. In addition, chicken Ch-MSC was induced to differentiate into four types of mesodermal cells in vitro, including osteoblasts, chondrocytes, adipocytes, and myoblasts. Therefore, the differentiation potential of chicken Ch-MSC in vitro may have great potential in tissue engineering. In conclusion, chicken Ch-MSCs may be an excellent model cell for stem cell regenerative medicine and chorionic tissue engineering.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140537941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic programming of stochastic olfactory receptor choice 随机嗅觉受体选择的表观遗传编程

IF 1.5 4区生物学

genesis Pub Date : 2024-04-01 DOI: 10.1002/dvg.23593

Nusrath Yusuf, Kevin Monahan

引用次数: 0

Signaling mechanisms underlying activity-dependent integration of adult-born neurons in the mouse olfactory bulb 小鼠嗅球中成体神经元活动依赖性整合的信号机制

IF 1.5 4区生物学

genesis Pub Date : 2024-03-30 DOI: 10.1002/dvg.23595

Suyang Bao, Juan M. Romero, Benjamin D. W. Belfort, Benjamin R. Arenkiel

{"title":"Signaling mechanisms underlying activity-dependent integration of adult-born neurons in the mouse olfactory bulb","authors":"Suyang Bao, Juan M. Romero, Benjamin D. W. Belfort, Benjamin R. Arenkiel","doi":"10.1002/dvg.23595","DOIUrl":"10.1002/dvg.23595","url":null,"abstract":"<div>\u0000 \u0000 Adult neurogenesis has fascinated the field of neuroscience for decades given the prospects of harnessing mechanisms that facilitate the rewiring and/or replacement of adult brain tissue. The subgranular zone of the hippocampus and the subventricular zone of the lateral ventricle are the two main areas in the brain that exhibit ongoing neurogenesis. Of these, adult-born neurons within the olfactory bulb have proven to be a powerful model for studying circuit plasticity, providing a broad and accessible avenue into neuron development, migration, and continued circuit integration within adult brain tissue. This review focuses on some of the recognized molecular and signaling mechanisms underlying activity-dependent adult-born neuron development. Notably, olfactory activity and behavioral states contribute to adult-born neuron plasticity through sensory and centrifugal inputs, in which calcium-dependent transcriptional programs, local translation, and neuropeptide signaling play important roles. This review also highlights areas of needed continued investigation to better understand the remarkable phenomenon of adult-born neuron integration.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140327245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data- and theory-driven approaches for understanding paths of epithelial–mesenchymal transition 以数据和理论为导向，了解上皮-间充质转化的路径。

IF 1.5 4区生物学

genesis Pub Date : 2024-03-29 DOI: 10.1002/dvg.23591

Tian Hong, Jianhua Xing

引用次数: 0