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A New Targeted Transgenic Mouse Line for the Study of Protocadherin γC4 研究原钙粘蛋白γ - c4的新靶向转基因小鼠系
IF 2.4 4区 生物学
genesis Pub Date : 2025-02-09 DOI: 10.1002/dvg.70010
Cathy M. McLeod, Camille M. Hanes, Leah C. Fuller, Samjhana Bhandari, Hannah G. Lanthier, Robert W. Burgess, Joshua A. Weiner, Andrew M. Garrett
{"title":"A New Targeted Transgenic Mouse Line for the Study of Protocadherin γC4","authors":"Cathy M. McLeod,&nbsp;Camille M. Hanes,&nbsp;Leah C. Fuller,&nbsp;Samjhana Bhandari,&nbsp;Hannah G. Lanthier,&nbsp;Robert W. Burgess,&nbsp;Joshua A. Weiner,&nbsp;Andrew M. Garrett","doi":"10.1002/dvg.70010","DOIUrl":"https://doi.org/10.1002/dvg.70010","url":null,"abstract":"<div>\u0000 \u0000 <p>The γ-protocadherins (γ-Pcdhs) comprise 22 homophilic cell adhesion molecule isoforms, expressed from the <i>Pcdhg</i> gene cluster via promoter choice mechanisms that serve many crucial functions during neural development. Emerging evidence supports the hypothesis that distinct isoforms have unique functions. The γC4 isoform, which is expressed from the <i>Pcdhgc4</i> promoter and includes its unique variable exon, is the sole γ-Pcdh isoform essential for the postnatal survival in mice. Here we describe a new mouse line (<i>C4-GFP</i>) in which <i>Pcdhgc4</i> with a C-terminal GFP tag is expressed from the <i>Rosa26</i> locus following excision of a lox-Stop-lox cassette by Cre recombinase. We report that restricted expression of this transgene in the nervous system using <i>Nestin-Cre</i> is sufficient to rescue the neonatal lethality of mice mutant for <i>Pcdhgc4</i>. This new line will be a vital tool for dissecting mechanisms underlying the functions of this essential cell adhesion molecule gene, mutations in which have been associated with neurodevelopmental disorders in humans.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"63 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Meet Our Editorial Board—Genesis. An Interview With, Susan Mackem, National Cancer Institute, Maryland, USA 见见我们的编辑委员会——创世纪。采访美国马里兰州国家癌症研究所的苏珊·麦克姆。
IF 2.4 4区 生物学
genesis Pub Date : 2025-01-29 DOI: 10.1002/dvg.70011
Paul Trevorrow, Susan Mackem
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引用次数: 0
Meet Our Editorial Board—Genesis. An Interview With Yevgenya Grinblat, University of Wisconsin-Madison, Wisconsin, USA 见见我们的编辑委员会——创世纪。美国威斯康辛大学麦迪逊分校Yevgenya Grinblat访谈
IF 2.4 4区 生物学
genesis Pub Date : 2025-01-27 DOI: 10.1002/dvg.70005
Paul Trevorrow, Yevgenya Grinblat
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引用次数: 0
Meet Our Editorial Board—Genesis. An Interview With Thomas Schimmang, Institute for Biomedicine and Molecular Genetics, Valladolid, Spain 见见我们的编辑委员会——创世纪。采访托马斯·希曼，西班牙巴利亚多利德生物医学和分子遗传学研究所。
IF 2.4 4区 生物学
genesis Pub Date : 2025-01-27 DOI: 10.1002/dvg.70004
Paul Trevorrow, Thomas Schimmang
{"title":"Meet Our Editorial Board—Genesis. An Interview With Thomas Schimmang, Institute for Biomedicine and Molecular Genetics, Valladolid, Spain","authors":"Paul Trevorrow,&nbsp;Thomas Schimmang","doi":"10.1002/dvg.70004","DOIUrl":"10.1002/dvg.70004","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143048398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Meet Our Editorial Board—Genesis. An Interview With Paolo E. Forni, University at Albany, State University of New York, New York, USA 见见我们的编辑委员会——创世纪。采访美国纽约州立大学奥尔巴尼分校的保罗·e·福尼
IF 2.4 4区 生物学
genesis Pub Date : 2025-01-24 DOI: 10.1002/dvg.70002
Paul Trevorrow, Paolo E. Forni
{"title":"Meet Our Editorial Board—Genesis. An Interview With Paolo E. Forni, University at Albany, State University of New York, New York, USA","authors":"Paul Trevorrow,&nbsp;Paolo E. Forni","doi":"10.1002/dvg.70002","DOIUrl":"10.1002/dvg.70002","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143034681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Meet Our Editorial Board—Genesis. An Interview With Yoh-suke Mukouyama, National Institutes of Health, Maryland, USA 见见我们的编辑委员会——创世纪。访美国马里兰州国立卫生研究院的佑介·慕山
IF 2.4 4区 生物学
genesis Pub Date : 2025-01-24 DOI: 10.1002/dvg.70008
Yoh-suke Mukouyama, Paul Trevorrow
{"title":"Meet Our Editorial Board—Genesis. An Interview With Yoh-suke Mukouyama, National Institutes of Health, Maryland, USA","authors":"Yoh-suke Mukouyama,&nbsp;Paul Trevorrow","doi":"10.1002/dvg.70008","DOIUrl":"10.1002/dvg.70008","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143034682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Meet Our Editorial Board—Genesis. An Interview With, Sally Moody, The George Washington University School of Medicine and Health Sciences, USA 认识我们的编辑委员会--《创世纪》。专访美国乔治-华盛顿大学医学与健康科学学院莎莉-穆迪。
IF 2.4 4区 生物学
genesis Pub Date : 2024-12-31 DOI: 10.1002/dvg.70000
Paul Trevorrow, Sally A. Moody
{"title":"Meet Our Editorial Board—Genesis. An Interview With, Sally Moody, The George Washington University School of Medicine and Health Sciences, USA","authors":"Paul Trevorrow,&nbsp;Sally A. Moody","doi":"10.1002/dvg.70000","DOIUrl":"10.1002/dvg.70000","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Meet Our Editorial Board—Genesis. An Interview With Margot L. K. Williams, Baylor College of Medicine, Texas, USA 见见我们的编辑委员会——创世纪。采访美国德克萨斯州贝勒医学院的玛格特·l·k·威廉姆斯。
IF 2.4 4区 生物学
genesis Pub Date : 2024-12-17 DOI: 10.1002/dvg.70001
Paul Trevorrow, Margot L. K. Williams
{"title":"Meet Our Editorial Board—Genesis. An Interview With Margot L. K. Williams, Baylor College of Medicine, Texas, USA","authors":"Paul Trevorrow,&nbsp;Margot L. K. Williams","doi":"10.1002/dvg.70001","DOIUrl":"10.1002/dvg.70001","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Generation and Characterization of a TRIM21 Overexpressing Mouse Line TRIM21过表达小鼠品系的产生和特征描述
IF 2.4 4区 生物学
genesis Pub Date : 2024-11-01 DOI: 10.1002/dvg.23616
Lisa M. Mehlmann, Tracy F. Uliasz, Siu-Pok Yee, Deborah Kaback, Katie M. Lowther
{"title":"Generation and Characterization of a TRIM21 Overexpressing Mouse Line","authors":"Lisa M. Mehlmann,&nbsp;Tracy F. Uliasz,&nbsp;Siu-Pok Yee,&nbsp;Deborah Kaback,&nbsp;Katie M. Lowther","doi":"10.1002/dvg.23616","DOIUrl":"10.1002/dvg.23616","url":null,"abstract":"<p>Specific removal of a protein is a key to understanding its function. “Trim-Away” utilizes TRIM21, an antibody receptor and ubiquitin ligase, for acute and specific reduction of proteins. When TRIM21 is expressed in cells, introduction of a specific antibody causes rapid degradation of the targeted protein; however, TRIM21 is endogenously expressed in few cell types. We have generated a mouse line using CRISPR to insert a conditional overexpression cassette of TRIM21 into the safe harbor site, <i>Rosa26</i>. These conditionally-expressing mice can be bred to a wide variety of <i>Cre</i> mice to target cell-specific TRIM21 overexpression in different tissues. <i>Zp3</i><sup><i>Cre</i></sup> mice expressed TRIM21 protein specifically in oocytes, whereas <i>Hprt</i><sup><i>Cre</i></sup> mice expressed the protein globally. When TRIM21-overexpressing oocytes were microinjected with specific antibodies targeting either the IP<sub>3</sub> receptor or SNAP23, these proteins were effectively degraded. In addition, cortical neural cells from globally-overexpressing TRIM21 mice showed a dramatic reduction in IP<sub>3</sub> receptor protein within hours after electroporation of a specific antibody. These experiments confirm the effectiveness of the Trim-Away method for protein reduction. These mice should make a valuable addition to the broader research community, as a wide range of proteins and cell types can be studied using this method.</p>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23616","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142565116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression and Transcriptional Targets of TGFβ-RII in Paracentrotus lividus Larval Skeletogenesis TGFβ-RII 在红腹角雉幼体骨骼发生过程中的表达和转录靶标
IF 2.4 4区 生物学
genesis Pub Date : 2024-08-14 DOI: 10.1002/dvg.23614
Daniel Goloe, Tsvia Gildor, Smadar Ben-Tabou de-Leon
{"title":"Expression and Transcriptional Targets of TGFβ-RII in Paracentrotus lividus Larval Skeletogenesis","authors":"Daniel Goloe,&nbsp;Tsvia Gildor,&nbsp;Smadar Ben-Tabou de-Leon","doi":"10.1002/dvg.23614","DOIUrl":"10.1002/dvg.23614","url":null,"abstract":"<p>Organisms from the five kingdoms of life use minerals to harden their tissues and make teeth, shells and skeletons, in the process of biomineralization. The sea urchin larval skeleton is an excellent system to study the biological regulation of biomineralization and its evolution. The gene regulatory network (GRN) that controls sea urchin skeletogenesis is known in great details and shows similarity to the GRN that controls vertebrates' vascularization while it is quite distinct from the GRN that drives vertebrates' bone formation. Yet, transforming growth factor beta (TGF-β) signaling regulates both sea urchin and vertebrates' skeletogenesis. Here, we study the upstream regulation and identify transcriptional targets of TGF-β in the Mediterranean Sea urchin species, <i>Paracentrotus lividus.</i> TGF-βRII is transiently active in the skeletogenic cells downstream of vascular endothelial growth factor (VEGF) signaling, in <i>P. lividus</i>. Continuous perturbation of TGF-βRII activity significantly impairs skeletal elongation and the expression of key skeletogenic genes. Perturbation of TGF-βRII after skeletal initiation leads to a delay in skeletal elongation and minor changes in gene expression. TGF-β targets are distinct from its transcriptional targets during vertebrates' bone formation, suggesting that the role of TGF-β in biomineralization in these two phyla results from convergent evolution.</p>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23614","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141977034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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