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Generation and characterization of Chd7-iCreERT2-tdTomato mice Chd7-iCreERT2-tdTomato小鼠的产生和表征。
IF 1.5 4区 生物学
genesis Pub Date : 2023-11-22 DOI: 10.1002/dvg.23575
Zi'ang Han, Ze Wang, Zhuxi Huang, Weijun Feng
{"title":"Generation and characterization of Chd7-iCreERT2-tdTomato mice","authors":"Zi'ang Han,&nbsp;Ze Wang,&nbsp;Zhuxi Huang,&nbsp;Weijun Feng","doi":"10.1002/dvg.23575","DOIUrl":"10.1002/dvg.23575","url":null,"abstract":"<div>\u0000 \u0000 <p>Heterozygous mutation of <i>CHD7</i> gene causes a severe developmental disorder called CHARGE syndrome. In order to further explore the expression and function of <i>Chd7</i> in vivo, we generated a <i>Chd7-P2A-iCreERT2-P2A-tdTomato</i> (in short, <i>Chd7-CT-tdT</i>) knockin mouse line using the CRISPR/Cas9 technology. The specificity and efficiency of two knockin genetic elements were validated. The <i>Chd7-CT-tdT</i> reporter gene could accurately reflect both the dynamic expression pattern of endogenous <i>Chd7</i> during neurodevelopment and cell-type specific expression in the brain and eye. The recombination efficiency of <i>Chd7-CT-tdT</i> in postnatal cerebellum is very high. Moreover, lineage tracing experiment showed that <i>Chd7</i> is expressed in intestinal stem cells. In summary, the newly constructed <i>Chd7-CT-tdT</i> mouse line provide a useful tool to study the function of <i>Chd7</i>.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138292087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unveiling the impact of DNA methylation machinery: Dnmt1 and Dnmt3a in orchestrating oocyte development and cellular homeostasis 揭示DNA甲基化机制的影响:Dnmt1和Dnmt3a在协调卵母细胞发育和细胞稳态中的作用。
IF 1.5 4区 生物学
genesis Pub Date : 2023-11-20 DOI: 10.1002/dvg.23579
Fatma Uysal, Gozde Sukur, Nazlican Bozdemir, Ozgur Cinar
{"title":"Unveiling the impact of DNA methylation machinery: Dnmt1 and Dnmt3a in orchestrating oocyte development and cellular homeostasis","authors":"Fatma Uysal,&nbsp;Gozde Sukur,&nbsp;Nazlican Bozdemir,&nbsp;Ozgur Cinar","doi":"10.1002/dvg.23579","DOIUrl":"10.1002/dvg.23579","url":null,"abstract":"<div>\u0000 \u0000 <p>DNA methylation can be considered the most prominent in controlling the gene expression responsible for the balance between cell proliferation and cell death. In this study, we aimed to analyze the distinct contributions of Dnmt1 and Dnmt3a enzymes in oocyte maturation, survival, autophagy, reactive oxygen species (ROS) production, and compensation capacity of Dnmt3b and Dnmt3l enzymes in mouse oocytes. Following confirming the suppression of Dnmt1or Dnmt3a through siRNA application, the assessment involved immunofluorescence staining for Dnmts, 5mC, p62, and ROS levels. Cell death rates showed a noticeable increase while oocyte maturation rates exhibited significant reduction. Global DNA methylation showed a decline, concomitant with elevated p62 and ROS levels upon Dnmt1 or Dnmt3a knockdown. Remarkably, silencing of Dnmt1 led to an upsurge in Dnmt3a expression, whereas Dnmt3a knockdown triggered an increase in Dnmt1 levels. Furthermore, Dnmt3l expression exhibited a notable decrease after silencing of either Dnmt1 or Dnmt3a, while Dnmt3b levels remained comparable between control and siRNA-treated groups. Collectively, this study underscores the pivotal roles of Dnmt1 and Dnmt3a in orchestrating various facets of oocyte development, encompassing maturation, survival, autophagy, and ROS production. These findings offer valuable insights into the intricate regulatory network governed by DNA methylation machinery within the context of oocyte physiology.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138177657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bop1 is required to establish precursor domains of craniofacial tissues Bop1是颅面组织前体结构域建立所必需的。
IF 1.5 4区 生物学
genesis Pub Date : 2023-11-16 DOI: 10.1002/dvg.23580
Stephanie Keer, Karen M. Neilson, Helene Cousin, Himani D. Majumdar, Dominique Alfandari, Steven L. Klein, Sally A. Moody
{"title":"Bop1 is required to establish precursor domains of craniofacial tissues","authors":"Stephanie Keer,&nbsp;Karen M. Neilson,&nbsp;Helene Cousin,&nbsp;Himani D. Majumdar,&nbsp;Dominique Alfandari,&nbsp;Steven L. Klein,&nbsp;Sally A. Moody","doi":"10.1002/dvg.23580","DOIUrl":"10.1002/dvg.23580","url":null,"abstract":"<div>\u0000 \u0000 <p>Bop1 can promote cell proliferation and is a component of the Pes1-Bop1-WDR12 (PeBoW) complex that regulates ribosomal RNA processing and biogenesis. In embryos, however, <i>bop1</i> mRNA is highly enriched in the neural plate, cranial neural crest and placodes, and potentially may interact with Six1, which also is expressed in these tissues. Recent work demonstrated that during development, Bop1 is required for establishing the size of the tadpole brain, retina and cranial cartilages, as well as controlling neural tissue gene expression levels. Herein, we extend this work by assessing the effects of Bop1 knockdown at neural plate and larval stages. Loss of Bop1 expanded neural plate gene expression domains (<i>sox2</i>, <i>sox11</i>, <i>irx1</i>) and reduced neural crest (<i>foxd3</i>, <i>sox9</i>), placode (<i>six1</i>, <i>sox11</i>, <i>irx1</i>, <i>sox9</i>) and epidermal (<i>dlx5</i>) expression domains. At larval stages, Bop1 knockdown reduced the expression of several otic vesicle genes (<i>six1</i>, <i>pax2</i>, <i>irx1</i>, <i>sox9</i>, <i>dlx5</i>, <i>otx2</i>, <i>tbx1</i>) and branchial arch genes that are required for chondrogenesis (<i>sox9</i>, <i>tbx1</i>, <i>dlx5</i>). The latter was not the result of impaired neural crest migration. Together these observations indicate that Bop1 is a multifunctional protein that in addition to its well-known role in ribosomal biogenesis functions during early development to establish the craniofacial precursor domains.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136399934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
EMT and primary ciliogenesis: For better or worse in sickness and in health EMT和原发性纤毛生成：无论疾病和健康状况如何。
IF 1.5 4区 生物学
genesis Pub Date : 2023-11-09 DOI: 10.1002/dvg.23568
Camille E. Tessier, Aurore M. M. Dupuy, Thomas Pelé, Philippe P. Juin, Jacqueline A. Lees, Vincent J. Guen
{"title":"EMT and primary ciliogenesis: For better or worse in sickness and in health","authors":"Camille E. Tessier,&nbsp;Aurore M. M. Dupuy,&nbsp;Thomas Pelé,&nbsp;Philippe P. Juin,&nbsp;Jacqueline A. Lees,&nbsp;Vincent J. Guen","doi":"10.1002/dvg.23568","DOIUrl":"10.1002/dvg.23568","url":null,"abstract":"<p>Epithelial-mesenchymal transition (EMT) and primary ciliogenesis are two cell-biological programs that are essential for development of multicellular organisms and whose abnormal regulation results in many diseases (i.e., developmental anomalies and cancers). Emerging studies suggest an intricate interplay between these two processes. Here, we discuss physiological and pathological contexts in which their interconnections promote normal development or disease progression. We describe underlying molecular mechanisms of the interplay and EMT/ciliary signaling axes that influence EMT-related processes (i.e., stemness, motility and invasion). Understanding the molecular and cellular mechanisms of the relationship between EMT and primary ciliogenesis may provide new insights in the etiology of diseases related to EMT and cilia dysfunction.</p>","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23568","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72015806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Signals, grids, and geometry: In pursuit of understanding cell fate switches. 信号、网格和几何：追求对细胞命运开关的理解。
IF 2.4 4区 生物学
genesis Pub Date : 2023-11-01 Epub Date: 2023-09-15 DOI: 10.1002/dvg.23546
Clare Hudson
{"title":"Signals, grids, and geometry: In pursuit of understanding cell fate switches.","authors":"Clare Hudson","doi":"10.1002/dvg.23546","DOIUrl":"10.1002/dvg.23546","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10264138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tunicate Eco-Evo-Devo laboratory in IMS-METU. 位于 IMS-METU 的 Tunicate Eco-Evo-Devo 实验室。
IF 2.4 4区 生物学
genesis Pub Date : 2023-11-01 Epub Date: 2023-07-11 DOI: 10.1002/dvg.23536
Arzu Karahan
{"title":"Tunicate Eco-Evo-Devo laboratory in IMS-METU.","authors":"Arzu Karahan","doi":"10.1002/dvg.23536","DOIUrl":"10.1002/dvg.23536","url":null,"abstract":"<p><p>I completed my undergraduate education in Atatürk University, Education Faculty, Biology Department. Then pursued my graduate education at the Biology Department of Mersin University. Both my master's and PhD theses were on the biological and population genetics features of various fish species. My initial encounter with tunicates dates back to my Postdoc at Israel Oceanographic and Limnologic Research Institute (IOLR) in 2011, where I was working on a DNA barcoding project. During that time, the entire institute was actively engaged in research on tunicates, and discussions during lunchtime often revolved around this fascinating group of organisms. Prof. Rinkevich usually only spoke seriously about tunicate biology but 1 day he told me \"You know Botryllus schlosseri is riding horse in Black Sea coasts of Turkiye.\" I was totally surprised and was trying to understand the meaning of this comment from a scientific perspective. He then showed me the picture of a B. schlosseri colony attached to a seahorse. Following several more Postdoc experiences, I began working as a Principal Investigator at Institute of Marine Sciences, Middle East Technical University (IMS-METU) in 2017. Since then, my team and I have been working on tunicate biodiversity, evolutionary biology, genomics, DNA barcoding, metabarcoding, metabolomics, whole-body regeneration (WBR) and aging related pathways.</p>","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9826504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Beryl Iris Brewin (1910-1999)-Accomplished New Zealand scientist and promoter of marine science. Beryl Iris Brewin（1910-1999 年）--杰出的新西兰科学家和海洋科学促进者。
IF 2.4 4区 生物学
genesis Pub Date : 2023-11-01 Epub Date: 2023-08-30 DOI: 10.1002/dvg.23533
Megan J Wilson
{"title":"Beryl Iris Brewin (1910-1999)-Accomplished New Zealand scientist and promoter of marine science.","authors":"Megan J Wilson","doi":"10.1002/dvg.23533","DOIUrl":"10.1002/dvg.23533","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10476950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Building hearts for undergraduate research with tunicates. 用石斑鱼为本科生的研究工作凝聚人心。
IF 2.4 4区 生物学
genesis Pub Date : 2023-11-01 Epub Date: 2023-08-16 DOI: 10.1002/dvg.23535
Heather J Evans Anderson
{"title":"Building hearts for undergraduate research with tunicates.","authors":"Heather J Evans Anderson","doi":"10.1002/dvg.23535","DOIUrl":"10.1002/dvg.23535","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10006532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regulatory mechanisms for sperm chemotaxis and flagellar motility. 精子趋化和鞭毛运动的调控机制
IF 2.4 4区 生物学
genesis Pub Date : 2023-11-01 Epub Date: 2023-09-12 DOI: 10.1002/dvg.23549
Kogiku Shiba
{"title":"Regulatory mechanisms for sperm chemotaxis and flagellar motility.","authors":"Kogiku Shiba","doi":"10.1002/dvg.23549","DOIUrl":"10.1002/dvg.23549","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10572166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The molecular basis of ascidian whole body regeneration. 腹足类全身再生的分子基础。
IF 2.4 4区 生物学
genesis Pub Date : 2023-11-01 Epub Date: 2023-07-14 DOI: 10.1002/dvg.23537
Megan J Wilson
{"title":"The molecular basis of ascidian whole body regeneration.","authors":"Megan J Wilson","doi":"10.1002/dvg.23537","DOIUrl":"10.1002/dvg.23537","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10029937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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