genesis最新文献_第4页

Epigenetic programming of stochastic olfactory receptor choice 随机嗅觉受体选择的表观遗传编程

IF 1.5 4区生物学

genesis Pub Date : 2024-04-01 DOI: 10.1002/dvg.23593

Nusrath Yusuf, Kevin Monahan

引用次数: 0

Signaling mechanisms underlying activity-dependent integration of adult-born neurons in the mouse olfactory bulb 小鼠嗅球中成体神经元活动依赖性整合的信号机制

IF 1.5 4区生物学

genesis Pub Date : 2024-03-30 DOI: 10.1002/dvg.23595

Suyang Bao, Juan M. Romero, Benjamin D. W. Belfort, Benjamin R. Arenkiel

{"title":"Signaling mechanisms underlying activity-dependent integration of adult-born neurons in the mouse olfactory bulb","authors":"Suyang Bao, Juan M. Romero, Benjamin D. W. Belfort, Benjamin R. Arenkiel","doi":"10.1002/dvg.23595","DOIUrl":"10.1002/dvg.23595","url":null,"abstract":"<div>\u0000 \u0000 <p>Adult neurogenesis has fascinated the field of neuroscience for decades given the prospects of harnessing mechanisms that facilitate the rewiring and/or replacement of adult brain tissue. The subgranular zone of the hippocampus and the subventricular zone of the lateral ventricle are the two main areas in the brain that exhibit ongoing neurogenesis. Of these, adult-born neurons within the olfactory bulb have proven to be a powerful model for studying circuit plasticity, providing a broad and accessible avenue into neuron development, migration, and continued circuit integration within adult brain tissue. This review focuses on some of the recognized molecular and signaling mechanisms underlying activity-dependent adult-born neuron development. Notably, olfactory activity and behavioral states contribute to adult-born neuron plasticity through sensory and centrifugal inputs, in which calcium-dependent transcriptional programs, local translation, and neuropeptide signaling play important roles. This review also highlights areas of needed continued investigation to better understand the remarkable phenomenon of adult-born neuron integration.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140327245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data- and theory-driven approaches for understanding paths of epithelial–mesenchymal transition 以数据和理论为导向，了解上皮-间充质转化的路径。

IF 1.5 4区生物学

genesis Pub Date : 2024-03-29 DOI: 10.1002/dvg.23591

Tian Hong, Jianhua Xing

引用次数: 0

An oocyte-specific Cas9-expressing mouse for germline CRISPR/Cas9-mediated genome editing 用于生殖系 CRISPR/Cas9 介导的基因组编辑的卵母细胞特异性 Cas9 表达小鼠。

IF 1.5 4区生物学

genesis Pub Date : 2024-03-24 DOI: 10.1002/dvg.23589

Denise G. Lanza, Jianqiang Mao, Isabel Lorenzo, Lan Liao, John R. Seavitt, M. Cecilia Ljungberg, Elizabeth M. Simpson, Francesco J. DeMayo, Jason D. Heaney

{"title":"An oocyte-specific Cas9-expressing mouse for germline CRISPR/Cas9-mediated genome editing","authors":"Denise G. Lanza, Jianqiang Mao, Isabel Lorenzo, Lan Liao, John R. Seavitt, M. Cecilia Ljungberg, Elizabeth M. Simpson, Francesco J. DeMayo, Jason D. Heaney","doi":"10.1002/dvg.23589","DOIUrl":"10.1002/dvg.23589","url":null,"abstract":"<div>\u0000 \u0000 <p>Cas9 transgenes can be employed for genome editing in mouse zygotes. However, using transgenic instead of exogenous Cas9 to produce gene-edited animals creates unique issues including ill-defined transgene integration sites, the potential for prolonged Cas9 expression in transgenic embryos, and increased genotyping burden. To overcome these issues, we generated mice harboring an oocyte-specific, <i>Gdf9</i> promoter driven, Cas9 transgene (Gdf9-Cas9) targeted as a single copy into the <i>Hprt1</i> locus. The X-linked <i>Hprt1</i> locus was selected because it is a defined integration site that does not influence transgene expression, and breeding of transgenic males generates obligate transgenic females to serve as embryo donors. Using microinjections and electroporation to introduce sgRNAs into zygotes derived from transgenic dams, we demonstrate that Gdf9-Cas9 mediates genome editing as efficiently as exogenous Cas9 at several loci. We show that genome editing efficiency is independent of transgene inheritance, verifying that maternally derived Cas9 facilitates genome editing. We also show that paternal inheritance of Gdf9-Cas9 does not mediate genome editing, confirming that Gdf9-Cas9 is not expressed in embryos. Finally, we demonstrate that off-target mutagenesis is equally rare when using transgenic or exogenous Cas9. Together, these results show that the Gdf9-Cas9 transgene is a viable alternative to exogenous Cas9.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140208044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deficits in olfactory system neurogenesis in neurodevelopmental disorders 神经发育障碍中嗅觉系统神经发生的缺陷。

IF 1.5 4区生物学

genesis Pub Date : 2024-03-15 DOI: 10.1002/dvg.23590

Sean C. Sweat, Claire E. J. Cheetham

引用次数: 0

Molecular mechanisms of differentiation and class choice of olfactory sensory neurons 嗅觉神经元分化和类别选择的分子机制

IF 1.5 4区生物学

genesis Pub Date : 2024-03-07 DOI: 10.1002/dvg.23587

Junji Hirota

{"title":"Molecular mechanisms of differentiation and class choice of olfactory sensory neurons","authors":"Junji Hirota","doi":"10.1002/dvg.23587","DOIUrl":"10.1002/dvg.23587","url":null,"abstract":"<p>The sense of smell is intricately linked to essential animal behaviors necessary for individual survival and species preservation. During vertebrate evolution, odorant receptors (ORs), responsible for detecting odor molecules, have evolved to adapt to changing environments, transitioning from aquatic to terrestrial habitats and accommodating increasing complex chemical environments. These evolutionary pressures have given rise to the largest gene family in vertebrate genomes. Vertebrate ORs are phylogenetically divided into two major classes; class I and class II. Class I OR genes, initially identified in fish and frog, have persisted across vertebrate species. On the other hand, class II OR genes are unique to terrestrial animals, accounting for ~90% of mammalian OR genes. In mice, each olfactory sensory neuron (OSN) expresses a single functional allele of a single OR gene from either the class I or class II OR repertoire. This one neuron-one receptor rule is established through two sequential steps: specification of OR class and subsequent exclusive OR expression from the corresponding OR class. Consequently, OSNs acquire diverse neuronal identities during the process of OSN differentiation, enabling animals to detect a wide array of odor molecules. This review provides an overview of the OSN differentiation process through which OSN diversity is achieved, primarily using the mouse as a model animal.</p>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23587","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140060881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Image, Volume 62, Issue 1 封面图片，第 62 卷第 1 期

IF 1.5 4区生物学

genesis Pub Date : 2024-02-26 DOI: 10.1002/dvg.23588

Mingyi Zhang, Jifan Feng, Yue Li, Peter Z. Qin, Yang Chai

引用次数: 0

Activity-dependent formation of the topographic map and the critical period in the development of mammalian olfactory system 活动依赖性地形图的形成和哺乳动物嗅觉系统发育的关键时期

IF 1.5 4区生物学

genesis Pub Date : 2024-01-30 DOI: 10.1002/dvg.23586

Ai Fang, C. Ron Yu

引用次数: 0

Cover Image, Volume 61, Issue 6 封面图片，第 61 卷第 6 期

IF 2.4 4区生物学

genesis Pub Date : 2023-12-31 DOI: 10.1002/dvg.23583

引用次数: 0

The Prl3d1-Cre mouse line selectively induces the expression of Cre recombinase in parietal trophoblast giant cells Prl3d1-Cre 小鼠品系可选择性地诱导顶体滋养层巨细胞中 Cre 重组酶的表达

IF 1.5 4区生物学

genesis Pub Date : 2023-12-20 DOI: 10.1002/dvg.23585

Linqing Pan, Fuquan Zhu, Aochen Yu, Yuan Jiang, Dayu Wang, Minglian Zhou, Chao Jia, Yugui Cui, Lisha Tang, Huaiyun Tang, Juan Li

{"title":"The Prl3d1-Cre mouse line selectively induces the expression of Cre recombinase in parietal trophoblast giant cells","authors":"Linqing Pan, Fuquan Zhu, Aochen Yu, Yuan Jiang, Dayu Wang, Minglian Zhou, Chao Jia, Yugui Cui, Lisha Tang, Huaiyun Tang, Juan Li","doi":"10.1002/dvg.23585","DOIUrl":"10.1002/dvg.23585","url":null,"abstract":"<div>\u0000 \u0000 <p>The placenta plays a pivotal role in the maintenance of normal pregnancy, but how it forms, matures, and performs its function remains poorly understood. Here, we describe a novel mouse line (Prl3d1-iCre) that expresses iCre recombinase under the control of the endogenous <i>prl3d1</i> promoter. Prl3d1 has been proposed as a marker for distinguishing trophoblast giant cells (TGCs) from other trophoblast cells in the placenta. The in vivo efficiency and specificity of the Cre line were analyzed by interbreeding Prl3d1-iCre mice with B6-G/R reporter mice. Through anatomical studies of the placenta and other tissues of Prl3d1-iCre/+; B6-G/R mouse mice, we found that the tdTomato signal was expressed in parietal trophoblast giant cells (P-TGCs). Thus, we report a mouse line with ectopic Cre expression in P-TGCs, which provides a valuable tool for studying human pathological pregnancies caused by implantation failure or abnormal trophoblast secretion due to aberrant gene regulation.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138825592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0