genesis最新文献

{"title":"Women in tunicate research: Pioneers of the past and their present legacy","authors":"Marie L. Nydam,&nbsp;Mary Beth Saffo,&nbsp;Anna Di Gregorio","doi":"10.1002/dvg.23578","DOIUrl":"10.1002/dvg.23578","url":null,"abstract":"<p>The search for female scientists who pioneered the research on tunicates is hindered by the tradition of reporting only the first initials of authors' names on scientific publications using only the initials of their first names. While this practice has the theoretical merit of broadening the readership by preventing the possible bias that could be caused by the gender of the author(s) in some of the readers, it rendered the identification of female researchers active in, or before, the first half of the 20th century quite challenging. Sifting through several dozen electronic records, and with the help of references and/or quotes found online, we have stitched together the information that we were able to retrieve on the life of female scientists who authored some of the earliest publications on tunicates, and we have organized them in (approximate) chronological order. We have also compiled brief synopses of the findings of scientists active in the field of tunicate biology in more recent times, and organized them by subdiscipline.</p>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11649620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Searching for marine embryos, finding my path","authors":"Anna Di Gregorio","doi":"10.1002/dvg.23576","DOIUrl":"10.1002/dvg.23576","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138296287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation and characterization of Chd7-iCreERT2-tdTomato mice","authors":"Zi'ang Han,&nbsp;Ze Wang,&nbsp;Zhuxi Huang,&nbsp;Weijun Feng","doi":"10.1002/dvg.23575","DOIUrl":"10.1002/dvg.23575","url":null,"abstract":"<div>\u0000 \u0000 <p>Heterozygous mutation of <i>CHD7</i> gene causes a severe developmental disorder called CHARGE syndrome. In order to further explore the expression and function of <i>Chd7</i> in vivo, we generated a <i>Chd7-P2A-iCreERT2-P2A-tdTomato</i> (in short, <i>Chd7-CT-tdT</i>) knockin mouse line using the CRISPR/Cas9 technology. The specificity and efficiency of two knockin genetic elements were validated. The <i>Chd7-CT-tdT</i> reporter gene could accurately reflect both the dynamic expression pattern of endogenous <i>Chd7</i> during neurodevelopment and cell-type specific expression in the brain and eye. The recombination efficiency of <i>Chd7-CT-tdT</i> in postnatal cerebellum is very high. Moreover, lineage tracing experiment showed that <i>Chd7</i> is expressed in intestinal stem cells. In summary, the newly constructed <i>Chd7-CT-tdT</i> mouse line provide a useful tool to study the function of <i>Chd7</i>.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138292087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1st International Symposium on Women in Tunicate Biology: Meeting report","authors":"Anna Di Gregorio,&nbsp;Marie L. Nydam","doi":"10.1002/dvg.23571","DOIUrl":"10.1002/dvg.23571","url":null,"abstract":"&lt;p&gt;The 1st International Symposium on Women in Tunicate Biology was held online on March 28 and 29, 2023. This global symposium was attended by 45–50 researchers from countries including Austria, Brazil, India, Italy, Japan, New Zealand, Turkey, and the United States. Figure 1 is a photograph of some of the participants from the March 28th session.&lt;/p&gt;&lt;p&gt;The main goals of this symposium were honoring women who advanced the field of tunicate biology, sharing current research interests, promoting collaborations, inspiring and supporting new and aspiring independent investigators, and fostering inclusivity. The symposium started its first day with biographical presentations of women scientists who pioneered the field, followed by tributes to recently retired female ascidiologists. The second day was mainly dedicated to presentations on the research currently being conducted by female principal investigators. On the first day, Anna Di Gregorio gave an introduction on the general history of tunicate research and then highlighted women who were active in the 19th and 20th centuries: Gladys Amelia Anslow from the United States, an accomplished physicist and first woman to work with the cyclotron at the University of California at Berkeley, who studied the effect of copper ions on ascidian metamorphosis; Helga Henriette Lindel Zwillenberg from Germany, who obtained the first images of chromosomes in ascidians through a method that she perfected for these organisms; Nel Krijgsman, from the Netherlands, who studied the pacemakers of the &lt;i&gt;Ciona&lt;/i&gt; heart and the effects of different neurotransmitters on their function, and Winifred Parsons, who studied the transport of carbon dioxide in the blood of ascidians and other vertebrates during her residence at the Stazione Zoologica in Napoli (Naples), Italy.&lt;/p&gt;&lt;p&gt;There were three talks honoring Italian women researchers. Fiorenza De Bernardi presented a tribute to Giuseppina Ortolani, who gained international recognition for her lineage-tracing experiments in solitary ascidians and trained numerous students who later became leaders in various fields of tunicate biology. Lucia Manni presented on her mentor Giovanna Zaniolo, another exceptionally talented experimentalist who pioneered studies of regeneration, allorecognition, and aging in colonial ascidians. Filomena Ristoratore and Annamaria Locascio described the research interests and accomplishments of recently retired women scientists from the Stazione Zoologica in Napoli: Margherita Branno, Anna Palumbo, Rosaria De Santis, and Elisabetta Tosti. Then, Megan Wilson introduced Beryl Brewin, a taxonomist and ecologist from New Zealand who worked for nearly 30 years at the University of Otago. Brewin described more than 80 ascidian genera and species from Australia and New Zealand and made a large financial donation in her will to support the Portobello Marine Laboratory. Megan also presented her lab's research, discussing in particular the insights her group ","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11649619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138177655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the impact of DNA methylation machinery: Dnmt1 and Dnmt3a in orchestrating oocyte development and cellular homeostasis","authors":"Fatma Uysal,&nbsp;Gozde Sukur,&nbsp;Nazlican Bozdemir,&nbsp;Ozgur Cinar","doi":"10.1002/dvg.23579","DOIUrl":"10.1002/dvg.23579","url":null,"abstract":"<div>\u0000 \u0000 <p>DNA methylation can be considered the most prominent in controlling the gene expression responsible for the balance between cell proliferation and cell death. In this study, we aimed to analyze the distinct contributions of Dnmt1 and Dnmt3a enzymes in oocyte maturation, survival, autophagy, reactive oxygen species (ROS) production, and compensation capacity of Dnmt3b and Dnmt3l enzymes in mouse oocytes. Following confirming the suppression of Dnmt1or Dnmt3a through siRNA application, the assessment involved immunofluorescence staining for Dnmts, 5mC, p62, and ROS levels. Cell death rates showed a noticeable increase while oocyte maturation rates exhibited significant reduction. Global DNA methylation showed a decline, concomitant with elevated p62 and ROS levels upon Dnmt1 or Dnmt3a knockdown. Remarkably, silencing of Dnmt1 led to an upsurge in Dnmt3a expression, whereas Dnmt3a knockdown triggered an increase in Dnmt1 levels. Furthermore, Dnmt3l expression exhibited a notable decrease after silencing of either Dnmt1 or Dnmt3a, while Dnmt3b levels remained comparable between control and siRNA-treated groups. Collectively, this study underscores the pivotal roles of Dnmt1 and Dnmt3a in orchestrating various facets of oocyte development, encompassing maturation, survival, autophagy, and ROS production. These findings offer valuable insights into the intricate regulatory network governed by DNA methylation machinery within the context of oocyte physiology.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138177657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating cellular dynamics in tunicates","authors":"Christina D. Cota","doi":"10.1002/dvg.23574","DOIUrl":"10.1002/dvg.23574","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138177656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bop1 is required to establish precursor domains of craniofacial tissues","authors":"Stephanie Keer,&nbsp;Karen M. Neilson,&nbsp;Helene Cousin,&nbsp;Himani D. Majumdar,&nbsp;Dominique Alfandari,&nbsp;Steven L. Klein,&nbsp;Sally A. Moody","doi":"10.1002/dvg.23580","DOIUrl":"10.1002/dvg.23580","url":null,"abstract":"<div>\u0000 \u0000 <p>Bop1 can promote cell proliferation and is a component of the Pes1-Bop1-WDR12 (PeBoW) complex that regulates ribosomal RNA processing and biogenesis. In embryos, however, <i>bop1</i> mRNA is highly enriched in the neural plate, cranial neural crest and placodes, and potentially may interact with Six1, which also is expressed in these tissues. Recent work demonstrated that during development, Bop1 is required for establishing the size of the tadpole brain, retina and cranial cartilages, as well as controlling neural tissue gene expression levels. Herein, we extend this work by assessing the effects of Bop1 knockdown at neural plate and larval stages. Loss of Bop1 expanded neural plate gene expression domains (<i>sox2</i>, <i>sox11</i>, <i>irx1</i>) and reduced neural crest (<i>foxd3</i>, <i>sox9</i>), placode (<i>six1</i>, <i>sox11</i>, <i>irx1</i>, <i>sox9</i>) and epidermal (<i>dlx5</i>) expression domains. At larval stages, Bop1 knockdown reduced the expression of several otic vesicle genes (<i>six1</i>, <i>pax2</i>, <i>irx1</i>, <i>sox9</i>, <i>dlx5</i>, <i>otx2</i>, <i>tbx1</i>) and branchial arch genes that are required for chondrogenesis (<i>sox9</i>, <i>tbx1</i>, <i>dlx5</i>). The latter was not the result of impaired neural crest migration. Together these observations indicate that Bop1 is a multifunctional protein that in addition to its well-known role in ribosomal biogenesis functions during early development to establish the craniofacial precursor domains.</p>\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136399934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Our motto: Ciona is beautiful!","authors":"Antonietta Spagnuolo,&nbsp;Nietta","doi":"10.1002/dvg.23564","DOIUrl":"10.1002/dvg.23564","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136399935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Women researchers in tunicate biology at the Stazione Zoologica Anton Dohrn in Napoli","authors":"Anna Di Gregorio,&nbsp;Annamaria Locascio,&nbsp;Filomena Ristoratore,&nbsp;Antonietta Spagnuolo","doi":"10.1002/dvg.23573","DOIUrl":"10.1002/dvg.23573","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134650249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene regulatory networks in ascidian embryos","authors":"Kaoru S. Imai","doi":"10.1002/dvg.23570","DOIUrl":"10.1002/dvg.23570","url":null,"abstract":"","PeriodicalId":12718,"journal":{"name":"genesis","volume":"61 6","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71523067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}