IF 1.5 4区生物学

genesis Pub Date : 2023-06-14 DOI: 10.1002/dvg.23527

Oginuma Masayuki, Anne-Cécile Reymann

{"title":"Meeting report: Third Franco-Japanese developmental biology meeting “New Frontiers in developmental biology: Celebrating the diversity of life”","authors":"Oginuma Masayuki, Anne-Cécile Reymann","doi":"10.1002/dvg.23527","DOIUrl":"10.1002/dvg.23527","url":null,"abstract":"The French and Japanese Developmental Biology Societies, teaming up with Human Frontier Science Program, were eager to meet back in person in November 2022 in the lovely city of Strasbourg. Top scientists in the developmental biology field from France and Japan, but also from United States, United Kingdom, Switzerland or Germany shared their exciting science during the 4 days of this meeting. Core fields of developmental biology such as morphogenesis, patterning, cell identity, and cell state transition, notably at the single cell level, were well represented, and a diversity of experimental models, including plants, animals, and other exotic organisms, as well as some in vitro cellular models, were covered. This event also extended the scope of classic scientific gatherings for two reasons. First the involvement of artists during the preparation of the event and on site. Second, part of the meeting was open for the general public through a series of outreach events, including a music and video presentation through projection mapping at Rohan palace, as well as public lectures.","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23527","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10351434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatio-temporal control of targeted gene expression in combination with CRISPR/Cas and Tet-On systems in Medaka 结合 CRISPR/Cas 和 Tet-On 系统对青鳉体内靶向基因表达进行时空控制

IF 1.5 4区生物学

genesis Pub Date : 2023-05-25 DOI: 10.1002/dvg.23519

Daichi Kayo, Sayaka Kimura, Touko Yamazaki, Kiyoshi Naruse, Hideaki Takeuchi, Satoshi Ansai

引用次数: 0

Histone acetyltransferases and histone deacetyl transferases play crucial role during oogenesis and early embryo development 组蛋白乙酰转移酶和组蛋白脱乙酰转移酶在卵子发生和胚胎早期发育过程中起着至关重要的作用。

IF 1.5 4区生物学

genesis Pub Date : 2023-05-25 DOI: 10.1002/dvg.23518

Nazlican Bozdemir, Fatma Uysal

{"title":"Histone acetyltransferases and histone deacetyl transferases play crucial role during oogenesis and early embryo development","authors":"Nazlican Bozdemir, Fatma Uysal","doi":"10.1002/dvg.23518","DOIUrl":"10.1002/dvg.23518","url":null,"abstract":"<div>\u0000 \u0000 Dynamic epigenetic regulation is critical for proper oogenesis and early embryo development. During oogenesis, fully grown germinal vesicle oocytes develop to mature Metaphase II oocytes which are ready for fertilization. Fertilized oocyte proliferates mitotically until blastocyst formation and the process is called early embryo development. Throughout oogenesis and early embryo development, spatio-temporal gene expression takes place, and this dynamic gene expression is controlled with the aid of epigenetics. Epigenetic means that gene expression can be altered without changing DNA itself. Epigenome is regulated through DNA methylation and histone modifications. While DNA methylation generally ends up with repression of gene expression, histone modifications can result in expression or repression depending on type of modification, type of histone protein and its specific residue. One of the modifications is histone acetylation which generally ends up with gene expression. Histone acetylation occurs through the addition of acetyl group onto amino terminal of the core histone proteins by histone acetyltransferases (HATs). Contrarily, histone deacetylation is associated with repression of gene expression, and it is catalyzed by histone deacetylases (HDACs). This review article focuses on what is known about alterations in the expression of HATs and HDACs and emphasizes importance of HATs and HDACs during oogenesis and early embryo development.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10298385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Cementum is key to periodontal tissue regeneration: A review on apatite microstructures for creation of novel cementum-based dental implants 牙骨质是牙周组织再生的关键：新型牙骨质基牙种植体的磷灰石微观结构综述。

IF 1.5 4区生物学

genesis Pub Date : 2023-04-17 DOI: 10.1002/dvg.23514

Mari M. Saito, Kazuo Onuma, Yasuo Yamakoshi

引用次数: 2

Generation of floxed Spag6l mice and disruption of the gene by crossing to a Hprt-Cre line 杂交Spag6l小鼠的产生和通过杂交到Hprt-Cre系对基因的破坏。

IF 1.5 4区生物学

genesis Pub Date : 2023-04-14 DOI: 10.1002/dvg.23512

Yonghong Man, Wei Li, Yi Tian Yap, Alivia Kearney, Siu-Pok Yee, Jerome F. Strauss III, Pamela Harding, Shizheng Song, Ling Zhang, Zhibing Zhang

{"title":"Generation of floxed Spag6l mice and disruption of the gene by crossing to a Hprt-Cre line","authors":"Yonghong Man, Wei Li, Yi Tian Yap, Alivia Kearney, Siu-Pok Yee, Jerome F. Strauss III, Pamela Harding, Shizheng Song, Ling Zhang, Zhibing Zhang","doi":"10.1002/dvg.23512","DOIUrl":"10.1002/dvg.23512","url":null,"abstract":"<div>\u0000 \u0000 Mouse sperm-associated antigen 6 like (SPAG6L) is an axoneme central apparatus protein, essential for the normal function of the ependymal cell and lung cilia, and sperm flagella. Accumulated evidence has disclosed multiple biological functions of SPAG6L, including ciliary/flagellar biogenesis and polarization, neurogenesis, and neuronal migration. Conventional Spag6l knockout mice died of hydrocephalus, which impedes further investigation of the function of the gene in vivo. To overcome the limitation of the short lifespan of conventional knockout mice, we developed a conditional allele by inserting two loxP sites in the genome flanking exon 3 of the Spag6l gene. By crossing the floxed Spag6l mice to a Hrpt-Cre line which expresses Cre recombinase ubiquitously in vivo, mutant mice that are missing SPAG6L globally were obtained. Homozygous mutant Spag6l mice showed normal appearance within the first week after birth, but reduced body size was observed after 1 week, and all developed hydrocephalus and died within 4 weeks of age. The phenotype mirrored that of the conventional Spag6l knockout mice. The newly established floxed Spag6l model provides a powerful tool to further investigate the role of the Spag6l gene in individual cell types and tissues.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10218432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterizing expression pattern of Six2Cre during mouse craniofacial development 表征小鼠颅面发育过程中Six2Cre的表达模式。

IF 1.5 4区生物学

genesis Pub Date : 2023-03-31 DOI: 10.1002/dvg.23516

Meenakshi Umar, Chunmin Dong, Fenglei He

引用次数: 0

A CRISPR/Cas9-engineered mouse carrying a conditional knockout allele for the early growth response-1 transcription factor 一种CRISPR/Cas9工程小鼠，携带早期生长应答-1转录因子的条件敲除等位基因。

IF 1.5 4区生物学

genesis Pub Date : 2023-03-22 DOI: 10.1002/dvg.23515

Vineet K. Maurya, Yan Ying, Denise G. Lanza, Jason D. Heaney, John P. Lydon

{"title":"A CRISPR/Cas9-engineered mouse carrying a conditional knockout allele for the early growth response-1 transcription factor","authors":"Vineet K. Maurya, Yan Ying, Denise G. Lanza, Jason D. Heaney, John P. Lydon","doi":"10.1002/dvg.23515","DOIUrl":"10.1002/dvg.23515","url":null,"abstract":"<div>\u0000 \u0000 Early growth response 1 (EGR1) mediates transcriptional programs that are indispensable for cell division, differentiation, and apoptosis in numerous physiologies and pathophysiologies. Whole-body EGR1 knockouts in mice (Egr1KO) have advanced our understanding of EGR1 function in an in vivo context. To extend the utility of the mouse to investigate EGR1 responses in a tissue- and/or cell-type-specific manner, we generated a mouse model in which exon 2 of the mouse Egr1 gene is floxed by CRISPR/Cas9 engineering. The floxed Egr1 alleles (Egr1f/f) are designed to enable spatiotemporal control of Cre-mediated EGR1 ablation in the mouse. To confirm that the Egr1f/f alleles can be abrogated using a Cre driver, we crossed the Egr1f/f mouse with a global Cre driver to generate the Egr1 conditional knockout (Egr1d/d) mouse in which EGR1 expression is ablated in all tissues. Genetic and protein analysis confirmed the absence of exon 2 and loss of EGR1 expression in the Egr1d/d mouse, respectively. Moreover, the Egr1d/d female exhibits overt reproductive phenotypes previously reported for the Egr1KO mouse. Therefore, studies described in this short technical report underscore the potential utility of the murine Egr1 floxed allele to further resolve EGR1 function at a tissue- and/or cell-type-specific level.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23515","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9857590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Generation of an OMgp allelic series in mice” 更正“在小鼠中产生OMgp等位基因系列”

IF 1.5 4区生物学

genesis Pub Date : 2023-03-20 DOI: 10.1002/dvg.23513

{"title":"Correction to “Generation of an OMgp allelic series in mice”","authors":"","doi":"10.1002/dvg.23513","DOIUrl":"10.1002/dvg.23513","url":null,"abstract":"\u0000 Lee, J.K., Case, L.C., Chan, A.F., Zhu, Y., Tessier-Lavigne, M. and Zheng, B. (2009), Generation of an OMgp allelic series in mice. Genesis, 47: 751–756. https://doi.org/10.1002/dvg.20557\u0000 In the originally published article, there are errors in the α-Tubulin controls on two Western blots presented in Figure 3a and Figure 4b. Two sets of gels, each in duplicate (a total of four gels), were run at the same time, and all were blotted sequentially with antibodies to OMgp and control α-Tubulin. While OMgp signals gave distinct patterns across different samples depending on the mutant conditions, control α-Tubulin signals gave similar patterns, which led to confusion and therefore errors in the figures in the specific lanes used for α-Tubulin controls. There were no errors for OMgp, which was the protein of interest. The authors have tracked the correct lanes for α-Tubulin controls, and the correct Western blot images for Figure 3a and Figure 4a, along with updated quantifications in Figure 3b and Figure 4b, is shown below. The figure legends remain the same for both figures except for the following: Representative data from one of 3–4 (instead of 3) independent sets of biological samples are shown. These errors had no impact on the scientific conclusions of the paper.We apologize for this error.","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23513","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9158641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using a modified piggyBac transposon-combined Cre/loxP system to produce selectable reporter-free transgenic bovine mammary epithelial cells for somatic cell nuclear transfer 利用改良的piggyBac转座子结合Cre/loxP系统制备可选择的无报告基因转基因牛乳腺上皮细胞用于体细胞核移植

IF 1.5 4区生物学

genesis Pub Date : 2023-02-07 DOI: 10.1002/dvg.23510

Guangdong Hu, Meijun Song, Yan Wang, Kexing Hao, Jing Wang, Yong Zhang

{"title":"Using a modified piggyBac transposon-combined Cre/loxP system to produce selectable reporter-free transgenic bovine mammary epithelial cells for somatic cell nuclear transfer","authors":"Guangdong Hu, Meijun Song, Yan Wang, Kexing Hao, Jing Wang, Yong Zhang","doi":"10.1002/dvg.23510","DOIUrl":"10.1002/dvg.23510","url":null,"abstract":"<div>\u0000 \u0000 Transposon systems are widely used for genetic engineering in various model organisms. PiggyBac (PB) has recently been confirmed to have highly efficient transposition in the mouse germ line and mammalian cell lines. In this study, we used a modified PB transposon system mediated by PB transposase (PBase) mRNA carrying the human lactoferrin gene driven by bovine β-casein promoter to transfect bovine mammary epithelial cells (BMECs), and the selectable reporter in two stable transgenic BMEC clones was removed using cell-permeant Cre recombinase. These reporter-free transgenic BMECs were used as donor cells for somatic cell nuclear transfer (SCNT) and exhibited a competence of SCNT embryos similar to stable transgenic BMECs and nontransgenic BMECs. The comprehensive information from this study provided a modified approach using an altered PB transposon system mediated by PBase mRNA in vitro and combined with the Cre/loxP system to produce transgenic and selectable reporter-free donor nuclei for SCNT. Consequently, the production of safe bovine mammary bioreactors can be promoted.\u0000 </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10279794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization 一株Ddx4-iCre转基因株系的产生及性状分析

IF 1.5 4区生物学

genesis Pub Date : 2023-01-24 DOI: 10.1002/dvg.23511

Guillaume Burnet, Chun-Wei Allen Feng, Ka Man Fiona Cheung, Josephine Bowles, Cassy M. Spiller

{"title":"Generation and characterization of a Ddx4-iCre transgenic line for deletion in the germline beginning at genital ridge colonization","authors":"Guillaume Burnet, Chun-Wei Allen Feng, Ka Man Fiona Cheung, Josephine Bowles, Cassy M. Spiller","doi":"10.1002/dvg.23511","DOIUrl":"10.1002/dvg.23511","url":null,"abstract":"Germline-specific Cre lines are useful for analyses of primordial germ cell, spermatogonial and oogonial development, but also for whole-body deletions when transmitted through subsequent generations. Several germ cell specific Cre mouse strains exist, with various degrees of specificity, efficiency, and temporal activation. Here, we describe the CRISPR/Cas9 targeted insertion of an improved Cre (iCre) sequence in-frame at the 3′ end of the Ddx4 locus to generate the Ddx4-P2A-iCre allele. Our functional assessment of this new allele, designated Ddx4iCreJoBo, reveals that Cre activity begins in PGCs from at least E10.5, and that it achieves higher efficiency for early gonadal (E10.5–12.5) germline deletion when compared to the inducible Oct4CreERT2 line. We found the Ddx4iCreJoBo allele to be hypomorphic for Ddx4 expression and homozygous males, but not females, were infertile. Using two reporter lines (R26RLacZ and R26RtdTomato) and a floxed gene of interest (Criptoflox) we found ectopic activity in multiple organs; global recombination (a common feature of germline Cre alleles) varies from 10 to 100%, depending on the particular floxed allele. There is a strong maternal effect, and therefore it is preferable for Ddx4iCreJoBo to be inherited from the male parent if ubiquitous deletion is not desired. With these limitations considered, we describe the Ddx4iCreJoBo line as useful for germline studies in which early gonadal deletion is required.","PeriodicalId":12718,"journal":{"name":"genesis","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvg.23511","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9202511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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