{"title":"Chd7-iCreERT2-tdTomato小鼠的产生和表征。","authors":"Zi'ang Han, Ze Wang, Zhuxi Huang, Weijun Feng","doi":"10.1002/dvg.23575","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Heterozygous mutation of <i>CHD7</i> gene causes a severe developmental disorder called CHARGE syndrome. In order to further explore the expression and function of <i>Chd7</i> in vivo, we generated a <i>Chd7-P2A-iCreERT2-P2A-tdTomato</i> (in short, <i>Chd7-CT-tdT</i>) knockin mouse line using the CRISPR/Cas9 technology. The specificity and efficiency of two knockin genetic elements were validated. The <i>Chd7-CT-tdT</i> reporter gene could accurately reflect both the dynamic expression pattern of endogenous <i>Chd7</i> during neurodevelopment and cell-type specific expression in the brain and eye. The recombination efficiency of <i>Chd7-CT-tdT</i> in postnatal cerebellum is very high. Moreover, lineage tracing experiment showed that <i>Chd7</i> is expressed in intestinal stem cells. In summary, the newly constructed <i>Chd7-CT-tdT</i> mouse line provide a useful tool to study the function of <i>Chd7</i>.</p>\n </div>","PeriodicalId":12718,"journal":{"name":"genesis","volume":"62 1","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Generation and characterization of Chd7-iCreERT2-tdTomato mice\",\"authors\":\"Zi'ang Han, Ze Wang, Zhuxi Huang, Weijun Feng\",\"doi\":\"10.1002/dvg.23575\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Heterozygous mutation of <i>CHD7</i> gene causes a severe developmental disorder called CHARGE syndrome. In order to further explore the expression and function of <i>Chd7</i> in vivo, we generated a <i>Chd7-P2A-iCreERT2-P2A-tdTomato</i> (in short, <i>Chd7-CT-tdT</i>) knockin mouse line using the CRISPR/Cas9 technology. The specificity and efficiency of two knockin genetic elements were validated. The <i>Chd7-CT-tdT</i> reporter gene could accurately reflect both the dynamic expression pattern of endogenous <i>Chd7</i> during neurodevelopment and cell-type specific expression in the brain and eye. The recombination efficiency of <i>Chd7-CT-tdT</i> in postnatal cerebellum is very high. Moreover, lineage tracing experiment showed that <i>Chd7</i> is expressed in intestinal stem cells. In summary, the newly constructed <i>Chd7-CT-tdT</i> mouse line provide a useful tool to study the function of <i>Chd7</i>.</p>\\n </div>\",\"PeriodicalId\":12718,\"journal\":{\"name\":\"genesis\",\"volume\":\"62 1\",\"pages\":\"\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2023-11-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"genesis\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/dvg.23575\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"genesis","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/dvg.23575","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
Generation and characterization of Chd7-iCreERT2-tdTomato mice
Heterozygous mutation of CHD7 gene causes a severe developmental disorder called CHARGE syndrome. In order to further explore the expression and function of Chd7 in vivo, we generated a Chd7-P2A-iCreERT2-P2A-tdTomato (in short, Chd7-CT-tdT) knockin mouse line using the CRISPR/Cas9 technology. The specificity and efficiency of two knockin genetic elements were validated. The Chd7-CT-tdT reporter gene could accurately reflect both the dynamic expression pattern of endogenous Chd7 during neurodevelopment and cell-type specific expression in the brain and eye. The recombination efficiency of Chd7-CT-tdT in postnatal cerebellum is very high. Moreover, lineage tracing experiment showed that Chd7 is expressed in intestinal stem cells. In summary, the newly constructed Chd7-CT-tdT mouse line provide a useful tool to study the function of Chd7.
期刊介绍：
As of January 2000, Developmental Genetics was renamed and relaunched as genesis: The Journal of Genetics and Development, with a new scope and Editorial Board. The journal focuses on work that addresses the genetics of development and the fundamental mechanisms of embryological processes in animals and plants. With increased awareness of the interplay between genetics and evolutionary change, particularly during developmental processes, we encourage submission of manuscripts from all ecological niches. The expanded numbers of genomes for which sequencing is being completed will facilitate genetic and genomic examination of developmental issues, even if the model system does not fit the “classical genetic” mold. Therefore, we encourage submission of manuscripts from all species. Other areas of particular interest include: 1) the roles of epigenetics, microRNAs and environment on developmental processes; 2) genome-wide studies; 3) novel imaging techniques for the study of gene expression and cellular function; 4) comparative genetics and genomics and 5) animal models of human genetic and developmental disorders.
genesis presents reviews, full research articles, short research letters, and state-of-the-art technology reports that promote an understanding of the function of genes and the roles they play in complex developmental processes.
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