{"title":"Generation and characterization of Chd7-iCreERT2-tdTomato mice","authors":"Zi'ang Han, Ze Wang, Zhuxi Huang, Weijun Feng","doi":"10.1002/dvg.23575","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Heterozygous mutation of <i>CHD7</i> gene causes a severe developmental disorder called CHARGE syndrome. In order to further explore the expression and function of <i>Chd7</i> in vivo, we generated a <i>Chd7-P2A-iCreERT2-P2A-tdTomato</i> (in short, <i>Chd7-CT-tdT</i>) knockin mouse line using the CRISPR/Cas9 technology. The specificity and efficiency of two knockin genetic elements were validated. The <i>Chd7-CT-tdT</i> reporter gene could accurately reflect both the dynamic expression pattern of endogenous <i>Chd7</i> during neurodevelopment and cell-type specific expression in the brain and eye. The recombination efficiency of <i>Chd7-CT-tdT</i> in postnatal cerebellum is very high. Moreover, lineage tracing experiment showed that <i>Chd7</i> is expressed in intestinal stem cells. In summary, the newly constructed <i>Chd7-CT-tdT</i> mouse line provide a useful tool to study the function of <i>Chd7</i>.</p>\n </div>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/dvg.23575","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
Abstract
Heterozygous mutation of CHD7 gene causes a severe developmental disorder called CHARGE syndrome. In order to further explore the expression and function of Chd7 in vivo, we generated a Chd7-P2A-iCreERT2-P2A-tdTomato (in short, Chd7-CT-tdT) knockin mouse line using the CRISPR/Cas9 technology. The specificity and efficiency of two knockin genetic elements were validated. The Chd7-CT-tdT reporter gene could accurately reflect both the dynamic expression pattern of endogenous Chd7 during neurodevelopment and cell-type specific expression in the brain and eye. The recombination efficiency of Chd7-CT-tdT in postnatal cerebellum is very high. Moreover, lineage tracing experiment showed that Chd7 is expressed in intestinal stem cells. In summary, the newly constructed Chd7-CT-tdT mouse line provide a useful tool to study the function of Chd7.
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