The Prl3d1-Cre mouse line selectively induces the expression of Cre recombinase in parietal trophoblast giant cells

IF 2.4 4区生物学 Q2 DEVELOPMENTAL BIOLOGY

genesis Pub Date : 2023-12-20 DOI:10.1002/dvg.23585

Linqing Pan, Fuquan Zhu, Aochen Yu, Yuan Jiang, Dayu Wang, Minglian Zhou, Chao Jia, Yugui Cui, Lisha Tang, Huaiyun Tang, Juan Li

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引用次数: 0

Abstract

The placenta plays a pivotal role in the maintenance of normal pregnancy, but how it forms, matures, and performs its function remains poorly understood. Here, we describe a novel mouse line (Prl3d1-iCre) that expresses iCre recombinase under the control of the endogenous prl3d1 promoter. Prl3d1 has been proposed as a marker for distinguishing trophoblast giant cells (TGCs) from other trophoblast cells in the placenta. The in vivo efficiency and specificity of the Cre line were analyzed by interbreeding Prl3d1-iCre mice with B6-G/R reporter mice. Through anatomical studies of the placenta and other tissues of Prl3d1-iCre/+; B6-G/R mouse mice, we found that the tdTomato signal was expressed in parietal trophoblast giant cells (P-TGCs). Thus, we report a mouse line with ectopic Cre expression in P-TGCs, which provides a valuable tool for studying human pathological pregnancies caused by implantation failure or abnormal trophoblast secretion due to aberrant gene regulation.

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Prl3d1-Cre 小鼠品系可选择性地诱导顶体滋养层巨细胞中 Cre 重组酶的表达

胎盘在维持正常妊娠中起着关键作用，但人们对胎盘如何形成、成熟和发挥其功能仍知之甚少。在这里，我们描述了一种新型小鼠品系（Prl3d1-iCre），该品系在内源性 prl3d1 启动子的控制下表达 iCre 重组酶。Prl3d1被认为是区分胎盘中滋养层巨细胞（TGC）和其他滋养层细胞的标志物。通过将 Prl3d1-iCre 小鼠与 B6-G/R 报告小鼠杂交，分析了 Cre 系的体内效率和特异性。通过对 Prl3d1-iCre/+; B6-G/R 小鼠胎盘和其他组织的解剖研究，我们发现tdTomato 信号在顶叶滋养层巨细胞（P-TGCs）中表达。因此，我们报告了一种在P-TGCs中异位表达Cre的小鼠品系，它为研究人类因植入失败或滋养层巨细胞分泌异常导致基因调控失常而引起的病理妊娠提供了一种有价值的工具。

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来源期刊

genesis 生物-发育生物学

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： As of January 2000, Developmental Genetics was renamed and relaunched as genesis: The Journal of Genetics and Development, with a new scope and Editorial Board. The journal focuses on work that addresses the genetics of development and the fundamental mechanisms of embryological processes in animals and plants. With increased awareness of the interplay between genetics and evolutionary change, particularly during developmental processes, we encourage submission of manuscripts from all ecological niches. The expanded numbers of genomes for which sequencing is being completed will facilitate genetic and genomic examination of developmental issues, even if the model system does not fit the “classical genetic” mold. Therefore, we encourage submission of manuscripts from all species. Other areas of particular interest include: 1) the roles of epigenetics, microRNAs and environment on developmental processes; 2) genome-wide studies; 3) novel imaging techniques for the study of gene expression and cellular function; 4) comparative genetics and genomics and 5) animal models of human genetic and developmental disorders. genesis presents reviews, full research articles, short research letters, and state-of-the-art technology reports that promote an understanding of the function of genes and the roles they play in complex developmental processes.