Free Radical Research最新文献_第2页

The interplay between microRNAs and oxidative stress and its implications in respiratory diseases. microrna与氧化应激的相互作用及其在呼吸系统疾病中的意义。

IF 2.9 3区生物学

Free Radical Research Pub Date : 2025-08-01 Epub Date: 2025-09-27 DOI: 10.1080/10715762.2025.2564674

Pushpendra Kumar Namdeo, Sehal Mishra, Amritha Das, Rajnarayan R Tiwari, Rajasekaran Subbiah

引用次数: 0

Unveiling the anti-aging activities and mechanism of melibiose: insights from metabolomics and RT-qPCR analyses. 从代谢组学和RT-qPCR分析揭示蜜利二糖的抗衰老活性及其机制

IF 2.9 3区生物学

Free Radical Research Pub Date : 2025-08-01 Epub Date: 2025-08-26 DOI: 10.1080/10715762.2025.2551848

Xu Chen, Jiazheng Liang, Tianyu Zheng, Zhijun Su, Shaojun Yu, Han Yu, Bo Wang, Yuanyuan Zhang, Yonggang Liu

{"title":"Unveiling the anti-aging activities and mechanism of melibiose: insights from metabolomics and RT-qPCR analyses.","authors":"Xu Chen, Jiazheng Liang, Tianyu Zheng, Zhijun Su, Shaojun Yu, Han Yu, Bo Wang, Yuanyuan Zhang, Yonggang Liu","doi":"10.1080/10715762.2025.2551848","DOIUrl":"10.1080/10715762.2025.2551848","url":null,"abstract":"In this study, the anti-aging potential of melibiose was examined, and its molecular mechanism was elucidated using Caenorhabditis elegans as a model organism. The findings demonstrated that melibiose at concentrations of 100 μM, 150 μM, and 200 μM significantly increased nematode lifespan by 15.38%, 23.08%, and 30.77% respectively. Additionally, melibiose enhanced resistance against heat and oxidative stress, improved nematode motility, reduced lipofuscin and reactive oxygen species (ROS) accumulation, and increased antioxidant enzyme activity. Through the use of gene-deletion nematodes, transgenic nematodes, RT-qPCR, and metabolomics, it was determined that melibiose potentially exerts its effects through multiple pathways including the insulin signaling pathway (down-regulation of daf-2 and age-1, up-regulation of sod-3 and hsp-16.2), the AMP-activated protein kinase (AMPK) pathway (up-regulation of aak-2), and the JNK pathway (up-regulation of jnk-1). Activation of transcription factors DAF-16, SKN-1, and HSF-1 was observed, moreover, delaying the aging process by promoting autophagy (upregulation of lgg-1 and bec-1) and mitochondrial function (upregulation of hsp-6, hsp-60, and mev-1) to resist oxidative damage. And its anti-aging signature metabolites may be Carbimazole, 4-Hydroxy-2-oxoglutaric acid, and 1,4-Dithiothreitol.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"617-633"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Far-infrared radiation-mediated GPx-1/eNOS/ERK signaling contributes to the protective potential against methamphetamine-caused impairments in recognition memory in mice. 远红外辐射介导的GPx-1/eNOS/ERK信号对甲基苯丙胺引起的小鼠识别记忆损伤有保护作用。

IF 2.9 3区生物学

Free Radical Research Pub Date : 2025-08-01 Epub Date: 2025-08-30 DOI: 10.1080/10715762.2025.2551032

Eun-Joo Shin, Yoon Hee Chung, Bao Trong Nguyen, Naveen Sharma, Ngoc Kim Cuong Tran, Yen Nhi Doan Nguyen, Jung Hoon Park, Dae-Joong Kim, Toshitaka Nabeshima, Ji Hoon Jeong, Hyoung-Chun Kim

{"title":"Far-infrared radiation-mediated GPx-1/eNOS/ERK signaling contributes to the protective potential against methamphetamine-caused impairments in recognition memory in mice.","authors":"Eun-Joo Shin, Yoon Hee Chung, Bao Trong Nguyen, Naveen Sharma, Ngoc Kim Cuong Tran, Yen Nhi Doan Nguyen, Jung Hoon Park, Dae-Joong Kim, Toshitaka Nabeshima, Ji Hoon Jeong, Hyoung-Chun Kim","doi":"10.1080/10715762.2025.2551032","DOIUrl":"10.1080/10715762.2025.2551032","url":null,"abstract":"Far-infrared radiation (FIR) induces glutathione peroxidase-1 (GPx-1) expression and enhances microcirculation by upregulating endothelial nitric oxide synthase (eNOS). However, the role of eNOS in FIR-mediated neuroprotection remains unclear. Here, we investigated whether FIR upregulates eNOS and extracellular signal-regulated kinase (ERK) signaling to mitigate recognition memory impairment caused by methamphetamine (MA). FIR significantly reduced MA-induced oxidative stress, which was primarily associated with GPx-1 inhibition. FIR or genetic overexpression of GPx-1 (GPx-1 TG) in mice significantly attenuated the MA-induced reduction in phospho-eNOS (p-eNOS) and phospho-ERK (p-ERK). Triple-label immunostaining revealed colocalization of p-eNOS, p-ERK, and GPx-1 within the same cellular populations in the prefrontal cortex. In non-transgenic (non-TG) mice, FIR exposure improved MA-induced cholinergic and memory deficits. However, FIR did not provide additional cognitive benefits in GPx-1 TG mice, and the GPx-1 inhibitor mercaptosuccinate blocked FIR-mediated cholinergic effects. Inhibitors of eNOS (i.e. L-NAME) and ERK (i.e. U0126) also significantly blocked the FIR-driven memory-enhancing effects in non-TG mice. Unlike L-NAME, which inhibits phosphorylation of both eNOS and ERK, U0126 did not affect FIR-induced eNOS phosphorylation, suggesting that eNOS is an upstream molecule for ERK signaling. Our finding suggests that GPx-1 is an essential mediator of FIR-induced memory enhancement, and that FIR exposure attenuates MA-induced cognitive impairments via cholinergic upregulation associated with GPx-1/eNOS/ERK signaling.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"592-616"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redox modulation by a synthetic thiol compound reduces LPS-induced pro-inflammatory cytokine expression in macrophages via AP-1/NLRP3 axis and influences the crosstalk with endothelial cells. 合成巯基化合物的氧化还原调节通过AP-1/NLRP3轴降低lps诱导的巨噬细胞中促炎细胞因子的表达，并影响与内皮细胞的串扰。

IF 2.9 3区生物学

Free Radical Research Pub Date : 2025-05-01 Epub Date: 2025-07-11 DOI: 10.1080/10715762.2025.2529914

Sofia Masini, Michela Bruschi, Michele Menotta, Barbara Canonico, Mariele Montanari, Daniela Ligi, Francesca Monittola, Ferdinando Mannello, Giovanni Piersanti, Rita Crinelli, Mauro Magnani, Alessandra Fraternale

{"title":"Redox modulation by a synthetic thiol compound reduces LPS-induced pro-inflammatory cytokine expression in macrophages via AP-1/NLRP3 axis and influences the crosstalk with endothelial cells.","authors":"Sofia Masini, Michela Bruschi, Michele Menotta, Barbara Canonico, Mariele Montanari, Daniela Ligi, Francesca Monittola, Ferdinando Mannello, Giovanni Piersanti, Rita Crinelli, Mauro Magnani, Alessandra Fraternale","doi":"10.1080/10715762.2025.2529914","DOIUrl":"10.1080/10715762.2025.2529914","url":null,"abstract":"Perturbation in redox status elicits multiple cellular pathways, including those involved in the inflammatory response. A thiol-based molecule (I-152), releasing N-acetyl-cysteine (NAC) and β-mercaptoethylamine (MEA), was exploited as a redox-modulating agent, and its effects on pro-inflammatory cytokine expression and secretion in lipopolysaccharide (LPS)-stimulated macrophages (MΦ) were investigated. I-152 inhibited cytokine gene expression as well as protein secretion of the most important inflammatory cytokines in three different MΦ models in vitro and ex vivo. It alleviated inflammation via the c-Jun/AP-1 and NF-κB signaling pathways, depending on the dose, and regulated NLRP3 inflammasome expression, leading to decreased IL-1β and IL-18 release and reduced pyroptotic cell death. Consequently, the influence of redox-modulated MΦ secretome on the crosstalk with endothelial cells was evaluated. Co-culture experiments between THP-1 MΦ, that had been pretreated with I-152 before LPS stimulation, and Human Vascular Endothelial Cells (HUVECs) showed reduced VCAM/ICAM expression in these cells in concomitance with a less oxidized and inflamed MΦ proteomic portrait. Overall, our findings suggest that I-152 redox modulation could target the AP-1/NLRP3 axis, affecting LPS-induced inflammation in MΦ and influencing HUVEC responses, revealing a complex and bidirectional interchange.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"487-505"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant properties of 34 alkaloids of natural origin: a density functional theory study. 34种天然生物碱的抗氧化性能：密度泛函理论研究。

IF 2.9 3区生物学

Free Radical Research Pub Date : 2025-05-01 Epub Date: 2025-07-31 DOI: 10.1080/10715762.2025.2539764

Andrey A Buglak, Taisiya A Telegina

引用次数: 0

Low-temperature plasma modulates seed germination through reactive oxygen species in dose-dependent manner. 低温等离子体通过活性氧以剂量依赖的方式调节种子萌发。

IF 2.9 3区生物学

Free Radical Research Pub Date : 2025-05-01 Epub Date: 2025-08-08 DOI: 10.1080/10715762.2025.2544800

Seungil Park, Bae Young Choi, You-Bin Seol, Jaewook Kim

{"title":"Low-temperature plasma modulates seed germination through reactive oxygen species in dose-dependent manner.","authors":"Seungil Park, Bae Young Choi, You-Bin Seol, Jaewook Kim","doi":"10.1080/10715762.2025.2544800","DOIUrl":"10.1080/10715762.2025.2544800","url":null,"abstract":"Atmospheric pressure low-temperature plasma treatment has been shown to enhance seed germination in various plant species. However, whether plasma treatment modulates seed dormancy status or affects the seed germination process remains unclear. Additionally, most studies have primarily focused on the positive effects of plasma on germination and growth, without addressing dose-dependent responses or underlying molecular mechanisms. To elucidate the effects of plasma treatment on seed germination at a molecular level, we analyzed the germination phenotype of fully ripened Arabidopsis thaliana seeds under germination-inhibitory conditions following plasma treatment. We observed that plasma treatment enhanced germination potential up to a critical threshold, beyond which prolonged treatment diminished the enhanced effect. Chemical staining assays identified that plasma treatment induced the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) at different time points. Machine-learning aided modeling revealed that ROS, rather than RNS, plays a key role in plasma-mediated germination induction. Furthermore, transcriptome analyses suggested candidate genes likely modulated by plasma treatment during seed germination, including glutathione and L-phenylalanine metabolism, abscisic acid signaling, and the tricarboxylic acid cycle. Our study provides the first molecular-level insights into how atmospheric pressure low-temperature plasma modulates seed germination.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"545-556"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urinary 8-oxo-7,8-dihydroguanosine as a potential biomarker for the prognosis of acute poisoning patients in the emergency Intensive-care unit: a prospective observational study. 尿8-氧-7,8-二氢鸟苷作为急诊重症监护病房急性中毒患者预后的潜在生物标志物：一项前瞻性观察研究

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-05-01 Epub Date: 2025-06-01 DOI: 10.1080/10715762.2025.2512463

Ya-Min Dang, Chao-Jie Chen, Ya-Qing Ma, Hong-Lei Liu, Wei Wen, Jin-Hua Quan, Ren-Ai Xu, Jun Dong, Zhong-Qiu Lu, Jian-Ping Cai

{"title":"Urinary 8-oxo-7,8-dihydroguanosine as a potential biomarker for the prognosis of acute poisoning patients in the emergency Intensive-care unit: a prospective observational study.","authors":"Ya-Min Dang, Chao-Jie Chen, Ya-Qing Ma, Hong-Lei Liu, Wei Wen, Jin-Hua Quan, Ren-Ai Xu, Jun Dong, Zhong-Qiu Lu, Jian-Ping Cai","doi":"10.1080/10715762.2025.2512463","DOIUrl":"10.1080/10715762.2025.2512463","url":null,"abstract":"Acute poisoning remains a significant cause of admission to the emergency intensive-care unit (EICU). Despite a reduced mortality rate, attention is increasingly focusing on the impact of post-intensive-care syndrome (PICS) on readmission. Due to the significant role of oxidative stress (OS) in the pathological mechanisms of poisoning, 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) may hold great potential as biomarkers for OS biomarkers to evaluate the severity and prognosis of poisoning. Therefore, we investigated the longitudinal changes of urinary 8-oxoGuo levels during hospitalization in poisoned patients, their association with organ failure, and their predictive value for 30-day readmission risk. In total, 43 poisoning patients were enrolled from the EICU of the First Affiliated Hospital of Wenzhou Medical University between July 2021 and November 2022. The 30-day readmission rate was 18.6%. Group-based trajectory modeling (GBTM) was used to explore the potential trajectories of urinary OS markers and organ failure scores during hospitalization. Spearman's correlation analysis revealed a significant association between a high trajectory of 8-oxoGuo/creatinine (Cr) and the increased severity of overall organ failure, as well as respiratory and coagulation dysfunctions. Binary logistic regression analysis indicated that a high 8-oxoGuo/Cr trajectory, high respiratory failure score trajectory, and 8-oxoGuo/Cr values at three key points in disease progression (including admission, transfer from EICU, and discharge), along with 8-oxodGuo/Cr levels at admission, were all risk factors for 30-day readmission. The 8-oxoGuo/Cr value at discharge exhibited the best predictive performance. The urinary 8-oxoGuo/Cr ratio may serve as a potential biomarker for prognostic evaluations in patients with poisoning.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"392-408"},"PeriodicalIF":3.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144157859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pro-inflammatory properties of M1 phenotypes of human macrophages: prolongation of myeloperoxidase-mediated oxidative stress. 人巨噬细胞M1表型的促炎特性：延长髓过氧化物酶介导的氧化应激。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-05-01 Epub Date: 2025-06-18 DOI: 10.1080/10715762.2025.2519528

Maria D Yurkanova, Nastasia V Kosheleva, Arina A Teplova, Peter S Timashev, Irina I Vlasova

{"title":"Pro-inflammatory properties of M1 phenotypes of human macrophages: prolongation of myeloperoxidase-mediated oxidative stress.","authors":"Maria D Yurkanova, Nastasia V Kosheleva, Arina A Teplova, Peter S Timashev, Irina I Vlasova","doi":"10.1080/10715762.2025.2519528","DOIUrl":"10.1080/10715762.2025.2519528","url":null,"abstract":"Macrophages and neutrophils are the main immune cells of the acute stage of inflammation. Upon their activation, membrane-bound NADPH oxidase produces superoxide anion radical, which is converted to H2O2 by superoxide dismutase (SOD). In this study, we compared the production of hydrogen peroxide by two phenotypes of pro-inflammatory human M1 macrophages and neutrophils activated with phorbol-12-myristate 13-acetate. Macrophages were obtained from blood monocytes (monocyte-derived macrophages (MDM)) differentiated into MDM using GM- or M-CSF growth factors and polarized into the M1 state, receiving GM_M1, M_M1, respectively. The total level of H2O2 production measured in the presence of horseradish peroxidase differed significantly between two types of macrophages. Only GM_M1 macrophages had a level of H2O2 production comparable to neutrophils. GM_M1 appear at the site of inflammation after neutrophils, they continue the work of neutrophils in creating a pro-inflammatory environment: they produce several times more H2O2 and pro-inflammatory cytokines than M_M1, which arrive at inflammatory site later. Upon activation, MDM_M1 formed big blot-like and smaller dense spheroid-like aggregates. Activated neutrophils secrete the enzyme myeloperoxidase (MPO), which synthesizes the very potent oxidant hypochlorous acid (HOCl) only in the presence of H2O2. Neutrophils are short lived cells, MPO can use H2O2 produced by activated cultured MDM to synthesize HOCl at physiologically relevant concentrations to prolong oxidative stress.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"452-461"},"PeriodicalIF":3.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilization of the nitroxyl radical TEMPOL to assess scavenging activities of lipid-soluble antioxidants against radicals initiated by the thermal decomposition of 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN) in ethanol. 利用硝基自由基TEMPOL评价脂溶性抗氧化剂对2,2'-偶氮唑（2,4-二甲基戊腈）（AMVN）在乙醇中热分解引发的自由基的清除能力。

IF 2.9 3区生物学

Free Radical Research Pub Date : 2025-05-01 Epub Date: 2025-07-22 DOI: 10.1080/10715762.2025.2531978

Keizo Takeshita, Ayaka Segawa, Kurumi Tokunaga, Ayaka Inamori, Ayako Matsuo, Yuhei Ohta, Shoko Okazaki

{"title":"Utilization of the nitroxyl radical TEMPOL to assess scavenging activities of lipid-soluble antioxidants against radicals initiated by the thermal decomposition of 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN) in ethanol.","authors":"Keizo Takeshita, Ayaka Segawa, Kurumi Tokunaga, Ayaka Inamori, Ayako Matsuo, Yuhei Ohta, Shoko Okazaki","doi":"10.1080/10715762.2025.2531978","DOIUrl":"10.1080/10715762.2025.2531978","url":null,"abstract":"To develop a simple and sensitive method for assessing the radical-scavenging activity of lipophilic antioxidants, the decay of the electron spin resonance (ESR) signal of 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl radical (TEMPOL) was investigated as an indicator of radical reactions. The ESR signal of TEMPOL was decreased in ethanol, but not in acetonitrile, by the pyrolysis of 2,2'-azobis(2,4-dimethylvaleronitrile). Signal decay in ethanol was suppressed by degassing and did not occur in the presence of the spin trapping agent 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO). Spin trapping with DEPMPO showed the formation of peroxyl, alkoxyl, and alkyl radical adducts during the reaction, with peroxyl radical adducts decreasing in the presence of TEMPOL. These results indicate that TEMPOL signal decay occurs by reactions involving ethanol-derived peroxyl radicals. The TEMPOL signal decay was remarkably inhibited by 0.01 mmol/L 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), which is lower than the Trolox concentration used in the ESR-spin trapping method reported previously. Inhibition by methyl gallate was markedly stronger than Trolox, while the effects of 2,6-di-tert-butyl-p-cresol and resveratrol were minor. The order of inhibition of TEMPOL signal decay by antioxidants correlated to some extent with the order of suppression of peroxyl radical adduct formation by DEPMPO spin trapping. Therefore, the peroxyl radical-scavenging activities of lipid-soluble antioxidants may be evaluated with high sensitivity by examining the inhibitory activity of TEMPOL decay caused by radical reactions in ethanol. The measurement time was less than 5 min.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"506-517"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of sinensetin against oxidative stress damage induced by AAPH in the brain-gut. 青肠素对AAPH诱导的脑-肠氧化应激损伤的保护作用。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-05-01 Epub Date: 2025-06-10 DOI: 10.1080/10715762.2025.2514799

Tingting Jin, Menghui He, Na Li, Ying He, Feng He

引用次数: 0