Free Radical Research最新文献_第2页

Dioscin induces ferroptosis to suppress the metastasis of gastric cancer through the SLC7A11/GPX4 axis. 薯蓣皂苷诱导铁下垂通过SLC7A11/GPX4轴抑制胃癌转移。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-05-01 Epub Date: 2025-06-13 DOI: 10.1080/10715762.2025.2515202

Doudou Lu, Ling Yuan, Zhaozhao Wang, Duojie Xu, Fandi Meng, Shumin Jia, Yahong Li, Weiqiang Li, Yi Nan

{"title":"Dioscin induces ferroptosis to suppress the metastasis of gastric cancer through the SLC7A11/GPX4 axis.","authors":"Doudou Lu, Ling Yuan, Zhaozhao Wang, Duojie Xu, Fandi Meng, Shumin Jia, Yahong Li, Weiqiang Li, Yi Nan","doi":"10.1080/10715762.2025.2515202","DOIUrl":"10.1080/10715762.2025.2515202","url":null,"abstract":"The prognosis of gastric cancer (GC) remains poor due to metastases and resistance to chemotherapy. Ferroptosis is a novel cell death regulation mode characterized by iron dependence and lipid peroxidation. Dioscin, a compound extracted from the Paris polyphylla rhizomes roots, has been shown to have an inhibitory effect on cancers. However, whether it induces ferroptosis to participate in anti-cancer metastasis remains unclear. The ability of gastric cancer cells to invade and migrate was evaluated by wound healing and transwell assays. Malondialdehyde (MDA), glutathione (GSH) assay kit, and dichlorofluorescin diacetate (DCFH-DA) fluorescent probes were used to detect ferroptosis in gastric cancer cells. The expression levels of glutathione peroxidase 4 (GPX4) and solute carrier family 7 member 11 (SLC7A11) were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot methods. The rescue assay was performed by adding Ferrostatin-1 (Fer-1) co-treatment to verify that Dioscin inhibited gastric cancer metastasis by participating in ferroptosis. Dioscin inhibited gastric cancer cells' wound healing, migration, and invasion process. In addition, Dioscin increased the level of reactive oxygen species (ROS) and MDA while decreasing GSH level, and induced ferroptosis of gastric cancer cells. Fer-1, an inhibitor of ferroptosis, could reverse the effect of Dioscin. In terms of mechanism, Dioscin induced ferroptosis through SLC7A11/GPX4 axis and was involved in the regulation of inhibiting metastasis of gastric cancer. These results suggested that Dioscin was involved in anti-cancer metastasis by inducing ferroptosis.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"426-441"},"PeriodicalIF":3.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenomethionine enhances transplant organ preservation by attenuating oxidative stress-induced proteolysis in rats. 硒代蛋氨酸通过减弱大鼠氧化应激诱导的蛋白水解而增强移植器官保存。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-05-01 Epub Date: 2025-06-11 DOI: 10.1080/10715762.2025.2516844

Paul Emir Hasuoka, Franco Tonelli, Leonardo Mariño-Repizo, Pablo Pacheco

{"title":"Selenomethionine enhances transplant organ preservation by attenuating oxidative stress-induced proteolysis in rats.","authors":"Paul Emir Hasuoka, Franco Tonelli, Leonardo Mariño-Repizo, Pablo Pacheco","doi":"10.1080/10715762.2025.2516844","DOIUrl":"10.1080/10715762.2025.2516844","url":null,"abstract":"Selenomethionine (SeMet) increases glutathione peroxidase (GPx) activity, a seleno-enzyme with an antioxidant function that counteracts reactive oxygen species (ROS). After ablation, transplant organs generate ROS during irrigation-reperfusion injury. GPx1 can be downregulated during hypoxia in ablated organs. ROS can oxidize proteins, inducing proteolysis, which compromises the transplant outcome. SeMet administration to living donors can decrease proteolysis in transplant organs, improving their preservation. Accordingly, SeMet was administered to rats for 7 days. After this period, the liver, heart, and kidneys were ablated, and proteins extracted at different postmortem intervals (PMI). Total protein analysis showed a lower protein concentration decrease in kidneys and heart from SeMet-supplemented rats after a 6 hs PMI. Molecular weight changes of proteins to proteolysis products (PPs) were studied by size exclusion chromatography (SEC). SeMet decreased PPs (<29.5 kDa) in the liver, kidneys, and heart. Specific analysis of GPx1 proteolysis by affinity chromatography coupled to inductively coupled plasma mass spectrometry (AF-ICP-MS) showed that SeMet administration decreased GPx1 proteolysis 24% in the liver and 16.8% in the heart. SeMet administration reduced the proteolysis velocity of GPx1 (VGPx1) in heart. SeMet administration to living donors for seven days decreased proteolysis in transplant organs, improving its conservation.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"442-451"},"PeriodicalIF":3.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial electron transport chain disruption and oxidative stress in lipopolysaccharide-induced cardiac dysfunction in rats and mice. 脂多糖诱导大鼠和小鼠心功能障碍的线粒体电子传递链断裂和氧化应激。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-05-01 Epub Date: 2025-05-15 DOI: 10.1080/10715762.2025.2503844

Agda Aline Pereira de Sousa, Leonardo da Silva Chaves, Heberty Tarso Facundo

{"title":"Mitochondrial electron transport chain disruption and oxidative stress in lipopolysaccharide-induced cardiac dysfunction in rats and mice.","authors":"Agda Aline Pereira de Sousa, Leonardo da Silva Chaves, Heberty Tarso Facundo","doi":"10.1080/10715762.2025.2503844","DOIUrl":"10.1080/10715762.2025.2503844","url":null,"abstract":"Sepsis, characterized by severe systemic inflammation and an excessive immune response to infection, is frequently triggered by bacterial endotoxins like lipopolysaccharide (LPS) from Gram-negative bacteria. Moreover, sepsis-induced cardiac dysfunction remains a leading cause of mortality. This study aims to elucidate the effects of LPS-induced cardiac injury on mitochondrial damage, oxidative stress, and subsequent cardiac dysfunction. LPS injections (in rats and mice) for three days (1.5 mg/kg) impacted the body weight and increased cardiac TNF-α. Additionally, it decreased mitochondrial complexes I and II activities while complexes III and IV remained unaffected. Disturbed in mitochondrial electron transport chain leads to an increase in reactive oxygen species (ROS). Indeed, LPS treatment significantly increased mitochondrial hydrogen peroxide production, reduced the activity of antioxidant enzymes catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase activity. This was accompanied by decreased mitochondrial and cytosolic sulfhydryl proteins and parallel increased cellular lipid peroxidation in the presence or absence of Fe2+. LPS-treated samples had increased glutathione s-transferase activity, which may be an attempt of the cell to remove toxic lipid peroxidation products. In a more acute Langendorff-perfused rat hearts, LPS infusion (0.5 μg/mL) induced a significant elevation in left ventricular end-diastolic pressure and a decrease in left ventricular developed pressure. These findings elucidate the harmful mitochondrial and oxidative effects of LPS in cardiac tissue and could help the development of targeted therapies to mitigate the adverse effects of sepsis-induced cardiac dysfunction.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"377-391"},"PeriodicalIF":3.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distal substituents effect of 4-oxo-TEMPO derivatives bearing a benzyl group at the 2-position on the reduction resistance toward ascorbate. 远端取代基2位含苄基的4-氧- tempo衍生物对抗坏血酸还原抗性的影响。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-05-01 Epub Date: 2025-05-16 DOI: 10.1080/10715762.2025.2503839

Karin Nagahama, Toshihide Yamasaki, Kohei Sano, Takahiro Mukai

{"title":"Distal substituents effect of 4-oxo-TEMPO derivatives bearing a benzyl group at the 2-position on the reduction resistance toward ascorbate.","authors":"Karin Nagahama, Toshihide Yamasaki, Kohei Sano, Takahiro Mukai","doi":"10.1080/10715762.2025.2503839","DOIUrl":"10.1080/10715762.2025.2503839","url":null,"abstract":"Nitroxides, which have unpaired electrons, find diverse applications owing to their characteristic redox and radical chemistries. To fine-tune the properties of nitroxide compounds for various applications, we investigated the effect of distant substituents on their reactivity. We synthesized 4-oxo-2,2,6,6-tetramethylpiperidine-N-oxyl (4-oxo-TEMPO) derivatives with electron-donating or electron-withdrawing groups at the para position of the benzyl group attached to the 2-position. The reactivities of these compounds were evaluated by measuring their second-order reaction rate constants with ascorbate using ESR spectroscopy. Density functional theory (DFT) calculations for each compound revealed a correlation between the charge of the N-O moiety and the second-order reaction rate constant. Notably, even substituents positioned far from the nitroxide center significantly affected the reductive reactivity with ascorbate. These findings suggest that both proximal and distal structural modifications can be leveraged to fine-tune nitroxide properties, providing a basis for the rational design of nitroxides with tailored reactivities.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"369-376"},"PeriodicalIF":3.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Edaravone inhibits neuronal ferroptosis and alleviates acute Central nervous system injury induced by diquat via enhancement of METTL14-mediated m6A methylation of Aldh1l1. 依达拉奉通过增强mettl14介导的Aldh1l1的m6A甲基化，抑制diquat诱导的神经元铁吊并减轻急性中枢神经系统损伤。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-03-01 Epub Date: 2025-03-28 DOI: 10.1080/10715762.2025.2482774

Liaozhang Wu, Zunwei Luo, Fuli Luo, Tingting Huang, Yifang Cen, Guosheng Rao, Zhijie Zhao, Renyang Ou, Manhong Zhou

{"title":"Edaravone inhibits neuronal ferroptosis and alleviates acute Central nervous system injury induced by diquat via enhancement of METTL14-mediated m6A methylation of Aldh1l1.","authors":"Liaozhang Wu, Zunwei Luo, Fuli Luo, Tingting Huang, Yifang Cen, Guosheng Rao, Zhijie Zhao, Renyang Ou, Manhong Zhou","doi":"10.1080/10715762.2025.2482774","DOIUrl":"10.1080/10715762.2025.2482774","url":null,"abstract":"The biological effects of edaravone (Eda), a free radical scavenger, include anti-inflammatory, antioxidant, and neuroprotective qualities. Nevertheless, the function and potential mechanisms of Eda in central nervous system injury damage are still unknown. A rat model of acute diquat toxicity was constructed to observe the pathological changes in brain tissues after diquat administration. The changes of mitophagy and ferroptosis in PC12 cells were assessed to the protective activity of Eda. To assess the methylation levels of m6A RNA, the EpiQuik m6A RNA Methylation Quantification Kit was utilized. RIP, dual luciferase reporter assay and mRNA stability detection confirm the relationship between METTL14 and Aldh11l1. Knockdown and overexpression experiments were performed to determine the effects of METTL14 and Aldh1l1 on rats and PC12 cells stimulated with diquat under Eda treatment. Eda dramatically ameliorated diquat-induced central nervous system injury. Eda notably attenuated apoptosis, pro-inflammatory cytokines activation, and oxidative stress damage in diquat-induced rats. Eda significantly suppressed apoptosis, mitophagy and ferroptosis after diquat-stimulated PC12 cells. Mitophagy inhibitor Mdivi-1 reversed the induction of ferroptosis effects of diquat via decreased Fe2+ content and increased Ca2+ level. knockdown of METTL14 reversed the therapeutic effect of Eda on diquat-induced injury. Eda promoted METTL14-mediated Aldh1l1 m6A methylation and alleviates acute central nervous system injury induced by diquat in vivo and in vitro. Eda has a protective effect on diquat-induced nervous system injury, and its mechanism may be related to the activation of m6A modification of Aldh11l1 by METTL14 and the inhibition of mitophagy and.ferroptosis.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"274-288"},"PeriodicalIF":3.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FABP3 promotes cell apoptosis and oxidative stress by regulating ferroptosis in lens epithelial cells. FABP3通过调节晶状体上皮细胞铁下垂促进细胞凋亡和氧化应激。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-03-01 Epub Date: 2025-03-13 DOI: 10.1080/10715762.2025.2475390

Qi Wang, Chunxiao Zhang, Bin Yu, Yanyan Zhang, Yuanyuan Guo

{"title":"FABP3 promotes cell apoptosis and oxidative stress by regulating ferroptosis in lens epithelial cells.","authors":"Qi Wang, Chunxiao Zhang, Bin Yu, Yanyan Zhang, Yuanyuan Guo","doi":"10.1080/10715762.2025.2475390","DOIUrl":"10.1080/10715762.2025.2475390","url":null,"abstract":"The purpose of this study is to investigate FABP3's biological function and potential mechanism in cataract. Treatment of H2O2 raised FABP3 expression. H2O2 decreased cell viability, enhanced apoptosis, promoted Bax and cleaved caspase-3 expression, inhibited Bcl-2 expression, enhanced the levels of IL-6, IL-1β, and TNF-α, raised MDA level, and decreased SOD and GSH levels in HLE-B3 cells. However, the effects of H2O2 on cell viability, apoptosis, inflammatory cytokines, and oxidative stress were reversed by FABP3 knockdown and aggravated by FABP3 overexpression. H2O2 increased the levels of lipid hydroperoxides and Fe2+, but reduced the expression of GPX4, SLC7A11, and Ferritin protein. Nevertheless, knockdown of FABP3 reversed the changes of lipid hydroperoxides, Fe2+, GPX4, SLC7A11, and Ferritin protein, and FABP3 overexpression caused the opposite results. In addition, the inhibition of FABP3 knockdown on cell apoptosis, inflammation, and oxidative stress was reversed by ferroptosis inducer (erastin), and the promotion of FABP3 overexpression on cell apoptosis, inflammation, and oxidative stress was reversed by ferroptosis inhibitor (Fer-1). Taken together, knockdown of FABP3 in lens epithelial cells treated with H2O2 restrained apoptosis, inflammation, and oxidative stress through regulating ferroptosis, suggesting that FABP3 might be a potential target for cataract treatment.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"250-261"},"PeriodicalIF":3.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Luteolin protects human ARPE-19 retinal pigment epithelium cells from blue light-induced phototoxicity through activation of Nrf2/Keap1 signaling. 木犀草素通过激活Nrf2/Keap1信号通路保护人ARPE-19视网膜色素上皮细胞免受蓝光诱导的光毒性。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-03-01 Epub Date: 2025-05-14 DOI: 10.1080/10715762.2025.2503832

Ryo Hayakawa, Takeshi Ishii, Taiki Fushimi, Yuki Kamei, Ai Yamaguchi, Kenji Sugimoto, Hitoshi Ashida, Mitsugu Akagawa

{"title":"Luteolin protects human ARPE-19 retinal pigment epithelium cells from blue light-induced phototoxicity through activation of Nrf2/Keap1 signaling.","authors":"Ryo Hayakawa, Takeshi Ishii, Taiki Fushimi, Yuki Kamei, Ai Yamaguchi, Kenji Sugimoto, Hitoshi Ashida, Mitsugu Akagawa","doi":"10.1080/10715762.2025.2503832","DOIUrl":"10.1080/10715762.2025.2503832","url":null,"abstract":"Age-related macular degeneration (AMD), a serious physical and mental health problem worldwide, is the leading cause of irreversible, severe vision impairment and loss in older people. AMD is associated with multiple risk factors, many of which are closely linked to increased oxidative stress. Some studies have suggested that long-term and excessive exposure to blue light may be a potential risk factor for the development or progression of AMD. Recently, we demonstrated that blue light irradiation caused oxidative stress in all-trans-retinal (atRAL)-exposed human ARPE-19 retinal pigment epithelium cells by generating singlet oxygen (1O2), leading to apoptotic cell death. Luteolin, a flavonoid found in various edible plants, has been reported to possess divergent health-promoting properties including anti-oxidative and chemopreventive effects by up-regulating anti-oxidative and phase II detoxifying enzymes through activation of Keap1/Nrf2 signaling. Herein, we verified the cytoprotective action of luteolin against blue light irradiation using atRAL-exposed ARPE-19 cells. Our results established that luteolin effectively prevented blue light-induced apoptosis of ARPE-19 cells by mitigating oxidative stress. We also confirmed that luteolin suppressed intracellular accumulation of 1O2 and formation of atRAL-derived lipofuscin by increased expression of heme oxygenase-1 and aldehyde dehydrogenase 1A1 through activation of Keap1/Nrf2 signaling. Furthermore, our data implied that the luteolin-provoked activation of Keap1/Nrf2 signaling might be due to covalent binding of luteolin o-quinone to the critical cysteinyl thiol in Keap1. The present results suggest that luteolin could be helpful in the prevention and amelioration of blue light-induced retinal degeneration, including AMD.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"356-368"},"PeriodicalIF":3.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cigarette smoke induces endoplasmic reticulum stress-associated mucus hypersecretion via orosomucoid 1-like protein 3 in airway epithelia. 香烟烟雾通过气道上皮口躯体样蛋白1- 3诱导内质网应激相关粘液高分泌。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-03-01 Epub Date: 2025-05-08 DOI: 10.1080/10715762.2025.2501019

Rui Chen, Yuting Cui, Mary Sau-Man Ip, Judith Choi-Wo Mak

{"title":"Cigarette smoke induces endoplasmic reticulum stress-associated mucus hypersecretion via orosomucoid 1-like protein 3 in airway epithelia.","authors":"Rui Chen, Yuting Cui, Mary Sau-Man Ip, Judith Choi-Wo Mak","doi":"10.1080/10715762.2025.2501019","DOIUrl":"10.1080/10715762.2025.2501019","url":null,"abstract":"Apart from a strong association with childhood-onset asthma, orosomucoid 1-like protein 3 (ORMDL3), an endoplasmic reticulum (ER)-localized transmembrane protein, is also linked with chronic obstructive pulmonary disease (COPD), in which cigarette smoke (CS) is the crucial risk factor. Compared to healthy subjects, COPD patients had elevated ORMDL3 mRNA in well-differentiated primary human bronchial epithelial cells (HBECs). However, its role in COPD remains understudied. We, therefore, hypothesize that ORMDL3 may play an essential role in CS-induced chronic mucus hypersecretion and inflammation via activation of specific unfolded protein response (UPR) pathways under ER stress in primary HBECs. Gene silencing using siRNA for ORMDL3 was performed in submerged culture of primary HBECs before 24-h cigarette smoke medium (CSM) exposure. The mucin, inflammatory and mitochondrial markers, and the activation of the UPR pathways were evaluated. CSM triggered significant induction of ORMDL3 expression at both mRNA and protein level, which was significantly inhibited by silencing ORMDL3. In addition, ORMDL3 knockdown inhibited CSM-induced mucin MUC5AC mRNA and release of inflammatory marker interleukin (IL)-8. Silencing ORMDL3 reduced CSM-induced ER stress via inhibiting the activating transcription factor (ATF)6 and the inositol-requiring enzyme (IRE)1 of the UPR pathways. The involvement of ORMDL3 was demonstrated in mitochondrial dynamics via fusion protein Mfn2 and mitochondrial respiration after CSM stimulation. In conclusion, ORMDL3 is an inducible gene in mediating CS-induced activation of specific ATF6 and IRE1 pathways to regulate mucus hypersecretion and inflammation. Therefore, ORMDL3 may be a promising therapeutic target to treat smoking-associated mucus hypersecretion and inflammation in COPD.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"342-355"},"PeriodicalIF":3.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactive oxygen species-mediated cytotoxic and DNA-damaging mechanism of N⁴-hydroxycytidine, a metabolite of the COVID-19 therapeutic drug molnupiravir. n4 -羟基胞苷是COVID-19治疗药物莫诺匹拉韦的代谢物，其活性氧介导的细胞毒性和dna损伤机制

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-03-01 Epub Date: 2025-03-06 DOI: 10.1080/10715762.2025.2469738

Yurie Mori, Rinya Yogo, Hatasu Kobayashi, Hirotaka Katsuzaki, Yuichiro Hirao, Shinya Kato, Hirokazu Kotani, Shosuke Kawanishi, Mariko Murata, Shinji Oikawa

{"title":"Reactive oxygen species-mediated cytotoxic and DNA-damaging mechanism of N4-hydroxycytidine, a metabolite of the COVID-19 therapeutic drug molnupiravir.","authors":"Yurie Mori, Rinya Yogo, Hatasu Kobayashi, Hirotaka Katsuzaki, Yuichiro Hirao, Shinya Kato, Hirokazu Kotani, Shosuke Kawanishi, Mariko Murata, Shinji Oikawa","doi":"10.1080/10715762.2025.2469738","DOIUrl":"10.1080/10715762.2025.2469738","url":null,"abstract":"Molnupiravir is a prodrug of the antiviral ribonucleoside analogue N4-hydroxycytidine (NHC), for use in the treatment of coronavirus disease 2019 (COVID-19). However, it is generally considered that NHC-triphosphate is incorporated into the host genome to induce mutations. In our previous preliminary report, we proposed oxidative DNA damage by NHC via cytidine deaminase (CDA)-mediated ROS formation. In the present study, we investigated cell viability using the HL-60 human leukemia cell line and its H2O2-resistant clone, HP100 cells. The survival rate was significantly reduced in HL-60 cells treated with NHC, but not in HP100 cells. LC-MS analysis revealed that uridine formation occurred from CDA-treated NHC, suggesting that CDA metabolizes NHC to uridine and hydroxylamine. We clarified mechanisms of CDA-mediated reactive oxygen species (ROS) generation and DNA damage by NHC using isolated DNA. CDA-treated NHC induced DNA damage in the presence of Cu(II). The DNA damage was enhanced by NADH addition and piperidine treatment. CDA-treated NHC and Cu(II) caused piperidine-labile sites at thymine, cytosine, and guanine, and the DNA cleavage pattern was similar to that of hydroxylamine. Catalase and bathocuproine inhibited the DNA damage, indicating the involvement of H2O2 and Cu(I). An indicator of oxidative DNA damage, 8-oxo-7,8-dihydro-2'-deoxyguanosine formation by CDA-treated NHC, was lower under hypoxic conditions than under normal conditions. Therefore, hydroxylamine, possibly produced from NHC treated with CDA, could induce metal-dependent H2O2 generation during the redox reactions, suggesting that oxidative DNA damage induced by ROS plays an important role in molnupiravir-related cytotoxicity and mutagenicity.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"205-214"},"PeriodicalIF":3.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examining the prognostic and clinicopathological significance of GPX4 in human cancers: a meta-analysis. GPX4在人类癌症中的预后和临床病理意义：一项荟萃分析。

IF 3.6 3区生物学

Free Radical Research Pub Date : 2025-03-01 Epub Date: 2025-03-06 DOI: 10.1080/10715762.2025.2475153

Hao Wu, Xiting Liao, Wusixian Huang, Huai Hu, Lan Lan, Qianlei Yang, Yan An

{"title":"Examining the prognostic and clinicopathological significance of GPX4 in human cancers: a meta-analysis.","authors":"Hao Wu, Xiting Liao, Wusixian Huang, Huai Hu, Lan Lan, Qianlei Yang, Yan An","doi":"10.1080/10715762.2025.2475153","DOIUrl":"10.1080/10715762.2025.2475153","url":null,"abstract":"Elevated levels of the enzyme GPX4 have been detected in tumor tissues, which may play a role in cancer progression. We did a meta-analysis of eight studies encompassing 1180 individuals to evaluate the importance of GPX4 in cancer, particularly in terms of prognosis and clinicopathological characteristics. Research results indicate that higher levels of GPX4 were linked to worse overall survival (OS) (HR = 1.47 [95%CI = 1.18-1.76], p < .001). Elevated levels of GPX4 were linked to lymph node invasion (OR.69 [95% CI.44-1.10], p =.12), metastasis (OR 1.58 [95% CI.97-2.55], p =.06, p <.0001), and advanced clinical stage III-IV (OR.82 [95% CI.70-.96], p =.001). A sensitivity study revealed that the general findings were constant across all levels of impact intensity. The findings of this meta-analysis suggest that increased GPX4 levels are not only correlated with reduced overall survival rates for patients with tumors but it also offers valuable insights regarding the clinical traits of tumor malignancy and metastasis. Based on these connections, GPX4 has the potential to serve as a biomarker for tumor detection, prognosis, and targeted therapy.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"239-249"},"PeriodicalIF":3.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0