从代谢组学和RT-qPCR分析揭示蜜利二糖的抗衰老活性及其机制

IF 2.9 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xu Chen, Jiazheng Liang, Tianyu Zheng, Zhijun Su, Shaojun Yu, Han Yu, Bo Wang, Yuanyuan Zhang, Yonggang Liu
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引用次数: 0

摘要

本研究以秀丽隐杆线虫为模型生物,探讨了蜜利二糖的抗衰老潜力,并阐明了其分子机制。结果表明,浓度为100 μM、150 μM和200 μM的蜜利二糖可使线虫的寿命分别延长15.38%、23.08%和30.77%。此外,蜜利二糖增强了线虫对高温和氧化应激的抵抗力,改善了线虫的运动能力,减少了脂褐素和活性氧(ROS)的积累,提高了抗氧化酶的活性。通过基因缺失线虫、转基因线虫、RT-qPCR和代谢组学研究,确定美利二糖可能通过胰岛素信号通路(下调daf-2和年龄-1,上调sod-3和hsp-16.2)、amp活化蛋白激酶(AMPK)通路(上调aak-2)、JNK通路(上调JNK -1)等多种途径发挥作用。此外,转录因子DAF-16、SKN-1和HSF-1被激活,并通过促进自噬(上调gg-1和bec1)和线粒体功能(上调hsp-6、hsp-60和mev-1)来延缓衰老过程,以抵抗氧化损伤。其抗衰老的标志性代谢物可能是咔马唑、4-羟基-2-氧戊二酸和1,4-二硫苏糖醇。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unveiling the Anti-aging Activities and Mechanism of Melibiose: Insights from Metabolomics and RT-qPCR Analyses.

In this study, the anti-aging potential of melibiose was examined, and its molecular mechanism was elucidated using Caenorhabditis elegans as a model organism. The findings demonstrated that melibiose at concentrations of 100 μM, 150 μM, and 200 μM significantly increased nematode lifespan by 15.38%, 23.08%, and 30.77% respectively. Additionally, melibiose enhanced resistance against heat and oxidative stress, improved nematode motility, reduced lipofuscin and reactive oxygen species (ROS) accumulation, and increased antioxidant enzyme activity. Through the use of gene-deletion nematodes, transgenic nematodes, RT-qPCR, and metabolomics, it was determined that melibiose potentially exerts its effects through multiple pathways including the insulin signaling pathway (down-regulation of daf-2 and age-1, up-regulation of sod-3 and hsp-16.2), the AMP-activated protein kinase (AMPK) pathway (up-regulation of aak-2), and the JNK pathway (up-regulation of jnk-1). Activation of transcription factors DAF-16, SKN-1, and HSF-1 was observed, moreover, delaying the aging process by promoting autophagy (upregulation of lgg-1 and bec-1) and mitochondrial function (upregulation of hsp-6, hsp-60, and mev-1) to resist oxidative damage. And its anti-aging signature metabolites may be Carbimazole, 4-Hydroxy-2-oxoglutaric acid, and 1,4-Dithiothreitol.

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来源期刊
Free Radical Research
Free Radical Research 生物-生化与分子生物学
CiteScore
6.70
自引率
0.00%
发文量
47
审稿时长
3 months
期刊介绍: Free Radical Research publishes high-quality research papers, hypotheses and reviews in free radicals and other reactive species in biological, clinical, environmental and other systems; redox signalling; antioxidants, including diet-derived antioxidants and other relevant aspects of human nutrition; and oxidative damage, mechanisms and measurement.
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