Xu Chen, Jiazheng Liang, Tianyu Zheng, Zhijun Su, Shaojun Yu, Han Yu, Bo Wang, Yuanyuan Zhang, Yonggang Liu
{"title":"从代谢组学和RT-qPCR分析揭示蜜利二糖的抗衰老活性及其机制","authors":"Xu Chen, Jiazheng Liang, Tianyu Zheng, Zhijun Su, Shaojun Yu, Han Yu, Bo Wang, Yuanyuan Zhang, Yonggang Liu","doi":"10.1080/10715762.2025.2551848","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, the anti-aging potential of melibiose was examined, and its molecular mechanism was elucidated using <i>Caenorhabditis elegans</i> as a model organism. The findings demonstrated that melibiose at concentrations of 100 μM, 150 μM, and 200 μM significantly increased nematode lifespan by 15.38%, 23.08%, and 30.77% respectively. Additionally, melibiose enhanced resistance against heat and oxidative stress, improved nematode motility, reduced lipofuscin and reactive oxygen species (ROS) accumulation, and increased antioxidant enzyme activity. Through the use of gene-deletion nematodes, transgenic nematodes, RT-qPCR, and metabolomics, it was determined that melibiose potentially exerts its effects through multiple pathways including the insulin signaling pathway (down-regulation of <i>daf-2</i> and <i>age-1</i>, up-regulation of <i>sod-3</i> and <i>hsp-16.2</i>), the AMP-activated protein kinase (AMPK) pathway (up-regulation of <i>aak-2</i>), and the JNK pathway (up-regulation of <i>jnk-1</i>). Activation of transcription factors DAF-16, SKN-1, and HSF-1 was observed, moreover, delaying the aging process by promoting autophagy (upregulation of <i>lgg-1</i> and <i>bec-1</i>) and mitochondrial function (upregulation of <i>hsp-6</i>, <i>hsp-60</i>, and mev-1) to resist oxidative damage. And its anti-aging signature metabolites may be Carbimazole, 4-Hydroxy-2-oxoglutaric acid, and 1,4-Dithiothreitol.</p>","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"1-17"},"PeriodicalIF":2.9000,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unveiling the Anti-aging Activities and Mechanism of Melibiose: Insights from Metabolomics and RT-qPCR Analyses.\",\"authors\":\"Xu Chen, Jiazheng Liang, Tianyu Zheng, Zhijun Su, Shaojun Yu, Han Yu, Bo Wang, Yuanyuan Zhang, Yonggang Liu\",\"doi\":\"10.1080/10715762.2025.2551848\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In this study, the anti-aging potential of melibiose was examined, and its molecular mechanism was elucidated using <i>Caenorhabditis elegans</i> as a model organism. The findings demonstrated that melibiose at concentrations of 100 μM, 150 μM, and 200 μM significantly increased nematode lifespan by 15.38%, 23.08%, and 30.77% respectively. Additionally, melibiose enhanced resistance against heat and oxidative stress, improved nematode motility, reduced lipofuscin and reactive oxygen species (ROS) accumulation, and increased antioxidant enzyme activity. Through the use of gene-deletion nematodes, transgenic nematodes, RT-qPCR, and metabolomics, it was determined that melibiose potentially exerts its effects through multiple pathways including the insulin signaling pathway (down-regulation of <i>daf-2</i> and <i>age-1</i>, up-regulation of <i>sod-3</i> and <i>hsp-16.2</i>), the AMP-activated protein kinase (AMPK) pathway (up-regulation of <i>aak-2</i>), and the JNK pathway (up-regulation of <i>jnk-1</i>). Activation of transcription factors DAF-16, SKN-1, and HSF-1 was observed, moreover, delaying the aging process by promoting autophagy (upregulation of <i>lgg-1</i> and <i>bec-1</i>) and mitochondrial function (upregulation of <i>hsp-6</i>, <i>hsp-60</i>, and mev-1) to resist oxidative damage. And its anti-aging signature metabolites may be Carbimazole, 4-Hydroxy-2-oxoglutaric acid, and 1,4-Dithiothreitol.</p>\",\"PeriodicalId\":12411,\"journal\":{\"name\":\"Free Radical Research\",\"volume\":\" \",\"pages\":\"1-17\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-08-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Free Radical Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/10715762.2025.2551848\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Free Radical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/10715762.2025.2551848","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Unveiling the Anti-aging Activities and Mechanism of Melibiose: Insights from Metabolomics and RT-qPCR Analyses.
In this study, the anti-aging potential of melibiose was examined, and its molecular mechanism was elucidated using Caenorhabditis elegans as a model organism. The findings demonstrated that melibiose at concentrations of 100 μM, 150 μM, and 200 μM significantly increased nematode lifespan by 15.38%, 23.08%, and 30.77% respectively. Additionally, melibiose enhanced resistance against heat and oxidative stress, improved nematode motility, reduced lipofuscin and reactive oxygen species (ROS) accumulation, and increased antioxidant enzyme activity. Through the use of gene-deletion nematodes, transgenic nematodes, RT-qPCR, and metabolomics, it was determined that melibiose potentially exerts its effects through multiple pathways including the insulin signaling pathway (down-regulation of daf-2 and age-1, up-regulation of sod-3 and hsp-16.2), the AMP-activated protein kinase (AMPK) pathway (up-regulation of aak-2), and the JNK pathway (up-regulation of jnk-1). Activation of transcription factors DAF-16, SKN-1, and HSF-1 was observed, moreover, delaying the aging process by promoting autophagy (upregulation of lgg-1 and bec-1) and mitochondrial function (upregulation of hsp-6, hsp-60, and mev-1) to resist oxidative damage. And its anti-aging signature metabolites may be Carbimazole, 4-Hydroxy-2-oxoglutaric acid, and 1,4-Dithiothreitol.
期刊介绍:
Free Radical Research publishes high-quality research papers, hypotheses and reviews in free radicals and other reactive species in biological, clinical, environmental and other systems; redox signalling; antioxidants, including diet-derived antioxidants and other relevant aspects of human nutrition; and oxidative damage, mechanisms and measurement.