Free Radical Research最新文献_第10页

Omaveloxolone ameliorates isoproterenol-induced pathological cardiac hypertrophy in mice. Omaveloxolone 可改善异丙肾上腺素诱导的小鼠病理性心肌肥大。

IF 3.3 3区生物学

Free Radical Research Pub Date : 2024-01-01 Epub Date: 2024-02-07 DOI: 10.1080/10715762.2023.2299359

Xianchao Li, Yang Wu, Yunzhao Yang, Yaohua Wu, Xi Yu, Wenjuan Hu

{"title":"Omaveloxolone ameliorates isoproterenol-induced pathological cardiac hypertrophy in mice.","authors":"Xianchao Li, Yang Wu, Yunzhao Yang, Yaohua Wu, Xi Yu, Wenjuan Hu","doi":"10.1080/10715762.2023.2299359","DOIUrl":"10.1080/10715762.2023.2299359","url":null,"abstract":"Nuclear factor erythroid 2-related factor 2 (Nrf2) is an important transcriptional regulator that plays a protective role against various cardiovascular diseases. Omaveloxolone is a newly discovered potent activator of Nrf2 that has a variety of cytoprotective functions. However, the potential role of omaveloxolone in the process of pathological cardiac hypertrophy and heart failure are still unknown. In this study, an isoproterenol (ISO)-induced pathological cardiac hypertrophy model was established to investigate the protective effect of omaveloxolone in vivo and in vitro. Our study first confirmed that omaveloxolone administration improved ISO-induced pathological cardiac hypertrophy in mice and neonatal cardiomyocytes. Omaveloxolone administration also diminished ISO-induced cardiac oxidative stress, inflammation and cardiomyocyte apoptosis. In addition, omaveloxolone administration activated the Nrf2 signaling pathway, and Nrf2 knockdown almost completely abolished the cardioprotective effect of omaveloxolone, indicated that the cardioprotective effect of omaveloxolone was directly related to the activation of the Nrf2 signaling. In summary, our study identified that omaveloxolone may be a promising therapeutic agent to mitigate pathological cardiac hypertrophy.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"57-68"},"PeriodicalIF":3.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in the antioxidant activity of metal-curcumin complexes: a combined computational and experimental review. 金属姜黄素复合物抗氧化活性的最新进展：计算与实验相结合的综述。

IF 3.3 3区生物学

Free Radical Research Pub Date : 2024-01-01 Epub Date: 2024-02-07 DOI: 10.1080/10715762.2023.2298857

Ankit Mittal, Mudita Nagpal, Vinod Kumar Vashistha, Richa Arora, Upasana Issar

引用次数: 0

Cemtirestat dimerization in liposomes and erythrocytes exposed to peroxyl radicals was reverted by thiol-disulfide exchange with GSH. 暴露于过氧自由基的脂质体和红细胞中的 Cemtirestat 二聚化可通过与 GSH 进行硫醇-二硫化物交换而恢复。

IF 3.3 3区生物学

Free Radical Research Pub Date : 2024-01-01 Epub Date: 2024-02-07 DOI: 10.1080/10715762.2023.2298852

Lucia Kovacikova, Marta S Prnova, Pavol Bodo, Milan Stefek

{"title":"Cemtirestat dimerization in liposomes and erythrocytes exposed to peroxyl radicals was reverted by thiol-disulfide exchange with GSH.","authors":"Lucia Kovacikova, Marta S Prnova, Pavol Bodo, Milan Stefek","doi":"10.1080/10715762.2023.2298852","DOIUrl":"10.1080/10715762.2023.2298852","url":null,"abstract":"In the model system of DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) liposomes exposed to peroxyl radicals generated by the azoinitiator AAPH, cemtirestat (CMTI-SH) inhibited lipid peroxidation more efficiently than the natural antioxidant glutathione. In the concentrations 100 to 500 µM, both CMTI-SH and GSH induced distinct lag phases in the initial stages of lipid peroxidation yet GSH produced consistently shorter induction periods (about twice) than equimolar CMTI-SH. Moreover, concentration dependence of lipid peroxidation inhibition measured at the 80th minute, revealed about three times higher IC50 value for GSH compared to CMTI-SH. When the incubations prolonged till 180 min no further absorbance changes at 270 and 302 nm, respectively, occurred. After addition of the reducing agent tris(2-carboxyethyl)phosphine, the absorbance peak at 270 nm shifted back to 302 nm. These findings pointed to the presence of reducible CMTI-SH disulfide whose definite structure was confirmed by proving identity of TLC retention and spectral data with those of the synthesized CMTI disulfide. When CMTI-SH and GSH were present simultaneously in the liposomal incubations, the mixing effect on the induction period was synergistic rather than additive. This was explained by ability of GSH to reduce CMTI disulfide which was proved in separate experiments with an authentic CMTI disulfide prepared synthetically. This finding was also demonstrated by experiment with CMTI-disulfide to protect the erythrocytes against oxidative damage induced by peroxyl radicals. To conclude, CMTI-SH scavenges reactive oxygen species yielding CMTI disulfide while GSH maintains CMTI-SH in the reduced state. This finding was also demonstrated by experiment with CMTI-disulfide to protect the erythrocytes against oxidative damage induced by peroxyl radicals. CMTI-SH would thus represent the first line of the cellular defense against peroxyl radical mediated oxidative stress.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"1-10"},"PeriodicalIF":3.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bis(1-methylimidazol-2-yl) diselenide and its evaluation as a chemical radio-protector: role of kinetic rate constants for ROS scavenging and glutathione peroxidase like activity. 双（1-甲基咪唑-2-基）二硒化物及其作为化学放射保护剂的评估：清除 ROS 的动力学速率常数和谷胱甘肽过氧化物酶活性的作用。

IF 3.3 3区生物学

Free Radical Research Pub Date : 2024-01-01 Epub Date: 2024-02-07 DOI: 10.1080/10715762.2023.2299341

K Makhijani, L B Kumbhare, M Nayak, A Kunwar, B G Singh

{"title":"Bis(1-methylimidazol-2-yl) diselenide and its evaluation as a chemical radio-protector: role of kinetic rate constants for ROS scavenging and glutathione peroxidase like activity.","authors":"K Makhijani, L B Kumbhare, M Nayak, A Kunwar, B G Singh","doi":"10.1080/10715762.2023.2299341","DOIUrl":"10.1080/10715762.2023.2299341","url":null,"abstract":"Bis(1-methylimidazol-2-yl) diselenide (MeImSe), a derivative of selenoneine, has been examined for bimolecular rate constants for scavenging of various radiolytically and non-radiolytically generated reactive oxygen species (ROS). Further, its potential to show glutathione peroxidase (GPx)-like activity and to protect in vitro models of DNA and lipid against radiation induced strand breakage and lipid peroxidation, respectively were studied. The results confirmed that MeImSe scavenged all major short-lived (hydroxyl radical) and long-lived (peroxyl radical, carbonate radical, nitrogen dioxide radical, hypochlorite and hydrogen peroxide) oxidants involved in the radiation toxicity either directly or through GPx-like catalytic mechanism. The rate constants of MeImSe for these oxidants were found to be comparable to analogous sulfur and selenium-based compounds. The enzyme kinetics study established that MeImSe took part in the GPx cycle through the reductive pathway. Further, MeImSe inhibited the radiation induced DNA strand cleavage and lipid peroxidation with half maximal inhibitory concentration (IC50) of ∼ 60 μM and ∼100 μM, respectively. Interestingly, MeImSe treatment in the above concentration range (>100 μM) did not show any significant toxicity in normal human lung fibroblast (WI26) cells. The balance between efficacy and toxicity of MeImSe as a chemical radioprotector was attributed to the formation of less reactive intermediates during its oxidation/reduction reactions as evidenced from NMR studies.HighlightsMeImSe, a derivative of selenoneine protects DNA and lipid from radiation damageMeImSe scavenges all major short- and long-lived oxidants involved in radiation toxicityRate constants of MeImSe for ROS scavenging determined by pulse radiolysis techniqueFirst organoselenium compound reported to scavenge nitrogen dioxide radicalMeImSe exhibits GPx-like activity through reductive pathway.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"43-56"},"PeriodicalIF":3.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139073823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacological inhibition of SIRT-2 by AK-7 modulates redox status and apoptosis via regulating Nrf2 in an experimental model of chronic obstructive pulmonary disease: an invivo and insilico study 在慢性阻塞性肺病实验模型中，AK-7 对 SIRT-2 的药理抑制可通过调节 Nrf2 调节氧化还原状态和细胞凋亡：一项体内和体外研究

IF 3.3 3区生物学

Free Radical Research Pub Date : 2023-12-04 DOI: 10.1080/10715762.2023.2288999

Vandana Yadav, Vinita Pandey, Atul Srivastava, Sangita Singh, Subhashini

引用次数: 0

Molecular targets for anti-oxidative protection of açaí berry against diabetes myocardial ischemia/reperfusion injury. açaí浆果抗糖尿病心肌缺血/再灌注损伤的分子靶点

IF 3.3 3区生物学

Free Radical Research Pub Date : 2023-05-01 DOI: 10.1080/10715762.2023.2243032

Daniela Impellizzeri, Marika Cordaro, Rosalba Siracusa, Roberta Fusco, Alessio Filippo Peritore, Enrico Gugliandolo, Tiziana Genovese, Rosalia Crupi, Livia Interdonato, Maurizio Evangelista, Rosanna Di Paola, Salvatore Cuzzocrea, Ramona D'Amico

{"title":"Molecular targets for anti-oxidative protection of açaí berry against diabetes myocardial ischemia/reperfusion injury.","authors":"Daniela Impellizzeri, Marika Cordaro, Rosalba Siracusa, Roberta Fusco, Alessio Filippo Peritore, Enrico Gugliandolo, Tiziana Genovese, Rosalia Crupi, Livia Interdonato, Maurizio Evangelista, Rosanna Di Paola, Salvatore Cuzzocrea, Ramona D'Amico","doi":"10.1080/10715762.2023.2243032","DOIUrl":"https://doi.org/10.1080/10715762.2023.2243032","url":null,"abstract":"Myocardial ischemia/reperfusion injury (MIRI) is the principal cause of death and occurs after prolonged blockage of the coronary arteries. Diabetes represents one of the main factors aggravating myocardial injury. Restoring blood flow is the first intervention against a heart attack, although reperfusion process could cause additional damage, such as the overproduction of reacting oxygen species (ROS). In recent years, açaí berry has gained international attention as a functional food due to its antioxidant and anti-inflammatory properties; not only that but this fruit has shown glucose-lowering effects. Therefore, this study was designed to evaluate the cardioprotective effects of açaí berry on the inflammatory and oxidative responses associated with diabetic MIRI. Diabetes was induced in rats by a single intravenous inoculation of streptozotocin (60 mg/kg) and allowed to develop for 60 days. MIRI was induced by occlusion of the left anterior descending coronary artery for 30 min followed by 2 h of reperfusion. Açaí (200 mg/kg) was administered 5 min before the end of ischemia and 1 h after reperfusion. In this study, we clearly demonstrated that açaí treatment was able to reduce biomarkers of myocardial damage, infarct size, and apoptotic process. Moreover, açaí administrations reduced inflammatory and oxidative response, modulating Nf-kB and Nrf2 pathways. These results suggest that açai berry supplementation could represent a useful strategy for pathological events associated to MIRI.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":"57 5","pages":"339-352"},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10282833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fullerene C₆₀ reduces acute lung injury by suppressing oxidative stress-mediated DMBA-induced apoptosis and autophagy by regulation of cytochrome-C/caspase-3/beclin-1/IL-1α/HO-1/p53 signaling pathways in rats. Fullerene C60通过调控细胞色素c /caspase-3/beclin-1/IL-1α/HO-1/p53信号通路，抑制氧化应激诱导的大鼠细胞凋亡和自噬，从而减轻急性肺损伤。

IF 3.3 3区生物学

Free Radical Research Pub Date : 2023-05-01 DOI: 10.1080/10715762.2023.2247555

Seda Beyaz, Abdullah Aslan, Ozlem Gok, Ibrahim Hanifi Ozercan, Can Ali Agca

{"title":"Fullerene C60 reduces acute lung injury by suppressing oxidative stress-mediated DMBA-induced apoptosis and autophagy by regulation of cytochrome-C/caspase-3/beclin-1/IL-1α/HO-1/p53 signaling pathways in rats.","authors":"Seda Beyaz, Abdullah Aslan, Ozlem Gok, Ibrahim Hanifi Ozercan, Can Ali Agca","doi":"10.1080/10715762.2023.2247555","DOIUrl":"https://doi.org/10.1080/10715762.2023.2247555","url":null,"abstract":"The objective of this study was to evaluate the effect of fullerene C60 nanoparticles against 7,12-dimethylbenz[a]anthracene (DMBA)-induced lung tissue damage in rats. 60 Wistar albino (8 weeks old) female rats were assigned into four groups: Control Group (C), Fullerene C60, DMBA, and Fullerene C60+DMBA. The rats in the DMBA and Fullerene C60+DMBA groups were administered DMBA (45 mg/kg bw, oral gavage). The rats in Fullerene C60, and Fullerene C60+DMBA groups were administered with Fullerene C60 (1.7 mg/kg bw, oral gavage). Expression levels of cytochrome-C, caspase-3, beclin-1, IL-1α, HO-1 and p53 proteins in lung tissue were determined by western blotting, lipid peroxidation malondialdehyde (MDA) analyzes, glutathione (GSH), glutathione peroxidase (GSH-Px), catalase activity (CAT) and total protein levels were determined by spectrophotometer. In addition, lung tissues were evaluated by histopathologically. Fullerene C60 reduced the increasing of MDA and IL-1α protein expression levels and attenuated histopathological changes in lung. Moreover, fullerene C60 enhanced the protein expression of cytochrome-C, caspase-3, beclin-1, HO-1, and p53, which were decreased in the DMBA group. Fullerene C60 has strong biological activity that it might be an effective approach for lung damage.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":"57 5","pages":"373-383"},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10285589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Germacrone protects renal tubular cells against ferroptotic death and ROS release by re-activating mitophagy in diabetic nephropathy. Germacrone通过重新激活糖尿病肾病中的线粒体自噬来保护肾小管细胞免受脱铁性死亡和ROS释放。

IF 3.3 3区生物学

Free Radical Research Pub Date : 2023-05-01 Epub Date: 2023-12-26 DOI: 10.1080/10715762.2023.2277143

Yunguang Wang, Xinxin He, Mengjiao Xue, Wenbo Sun, Qiang He, Juan Jin

{"title":"Germacrone protects renal tubular cells against ferroptotic death and ROS release by re-activating mitophagy in diabetic nephropathy.","authors":"Yunguang Wang, Xinxin He, Mengjiao Xue, Wenbo Sun, Qiang He, Juan Jin","doi":"10.1080/10715762.2023.2277143","DOIUrl":"10.1080/10715762.2023.2277143","url":null,"abstract":"Mitophagy is a critical intracellular event during the progression of diabetic nephropathy (DN). Our previous study demonstrated that germacrone has anti-ferroptotic properties and is a potential therapeutic agent for DN. However, the relationship among germacrone, mitophagy, and ferroptosis in DN remains unclear. In this study, the data confirmed that germacrone ameliorates high glucose (HG)-induced ferroptosis through limiting Fe (2+) content and lipid reactive oxygen species (ROS) accumulation in human kidney 2 (HK-2) cells. Germacrone reversed HG-mediated inhibition of mitophagy. Mitophagy inhibition and anabatic mitochondrial ROS abrogate germacrone-mediated protective effects against ferroptotic death, resulting in the subsequent activation of mitochondrial DNA (mtDNA) cytosolic leakage-induced stimulator of interferon response CGAMP interactor 1 (STING) signaling. The combination of a mitochondrial ROS antagonist and germacrone acts synergistically to alleviate the ferroptotic death of tubular cells and DN symptoms. In summary, germacrone ameliorated ferroptotic death in tubular cells by reactivating mitophagy and inhibiting mtDNA-STING signaling in DN. This study provides a novel insight into germacrone-mediated protection against DN progression and further confirms that antioxidant pharmacological strategies facilitate the treatment of DN.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"413-429"},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66783753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between serum superoxide dismutase and thyroid function in obese patients after Laparoscopic sleeve gastrectomy. 腹腔镜袖状胃切除术后肥胖患者血清超氧化物歧化酶与甲状腺功能的关系。

IF 3.3 3区生物学

Free Radical Research Pub Date : 2023-05-01 Epub Date: 2023-12-26 DOI: 10.1080/10715762.2023.2265054

Hui You, Xin Wen, Xingchun Wang, Cuiling Zhu, Manna Zhang, Le Bu, Haibing Chen, Chunjun Sheng, Shen Qu

{"title":"The relationship between serum superoxide dismutase and thyroid function in obese patients after Laparoscopic sleeve gastrectomy.","authors":"Hui You, Xin Wen, Xingchun Wang, Cuiling Zhu, Manna Zhang, Le Bu, Haibing Chen, Chunjun Sheng, Shen Qu","doi":"10.1080/10715762.2023.2265054","DOIUrl":"10.1080/10715762.2023.2265054","url":null,"abstract":"To investigate the cross-sectional and longitudinal correlation between serum superoxide dismutase (SOD) levels and thyroid function with obesity before and after laparoscopic sleeve gastrectomy (LSG). Patients with morbid obesity (n = 219, 112 males and 107 females) who underwent LSG were selected and they were subdivided into normal levels of SOD (NSOD, n = 112) and high levels of SOD (HSOD, n = 107) according to the median value of SOD levels (183 U/mL). SOD and thyroid hormones were measured and compared at baseline, 3, 6, and 12 months after LSG. The HSOD group had lower body mass index (BMI), total thyroxine (TT4), and thyroid-stimulating hormone (TSH) than the NSOD group (p < 0.001, p = 0.031, p < 0.001, respectively). However, they had higher free triiodothyronine (FT3) and free thyroxine (FT4) (p = 0.019 and p = 0.017, respectively). SOD was significantly negatively associated with TSH and positively associated with FT4. Of all the patients, 22.31% (NSOD: 66.67%; HSOD: 33.33%) had subclinical hypothyroidism (SH), and there were lower SOD levels in the SH group. Preoperative SOD was a protective factor for SH. After LSG, SOD and FT4 levels were increased at 12 months after LSG, however, TSH, FT3, total triiodothyronine (TT3) and TT4 levels decreased compared to the preoperative levels at 3, 6, and 12 months in the SH group. Postoperative changes in FT4 and TT4 levels correlated with changes in SOD levels. SOD, which is correlated with thyroid hormones, protects against SH in patients with obesity. The improvement in thyroid function with SH after LSG may be related to increased SOD levels.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"395-403"},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the nature of the chelated metal on the photodynamic activity of metalloporphyrins. 螯合金属的性质对金属卟啉光动力学活性的影响。

IF 3.3 3区生物学

Free Radical Research Pub Date : 2023-05-01 Epub Date: 2023-12-26 DOI: 10.1080/10715762.2023.2288997

Ghadeer Abbas, Fatemah Alibrahim, Rawan Kankouni, Sara Al-Belushi, Dalal A Al-Mutairi, Artak Tovmasyan, Ines Batinic-Haberle, Ludmil Benov

{"title":"Effect of the nature of the chelated metal on the photodynamic activity of metalloporphyrins.","authors":"Ghadeer Abbas, Fatemah Alibrahim, Rawan Kankouni, Sara Al-Belushi, Dalal A Al-Mutairi, Artak Tovmasyan, Ines Batinic-Haberle, Ludmil Benov","doi":"10.1080/10715762.2023.2288997","DOIUrl":"10.1080/10715762.2023.2288997","url":null,"abstract":"Coordination of metal ions by the tetrapyrrolic macrocyclic ring of porphyrin-based photosensitizers (PSs) affects their photophysical properties and consequently, their photodynamic activity. Diamagnetic metals increase the singlet oxygen quantum yield while paramagnetic metals have the opposite effect. Since singlet oxygen is considered the main cell-damaging species in photodynamic therapy (PDT), the nature of the chelated cation would directly affect PDT efficacy. This expectation, however, is not always supported by experimental results and numerous exceptions have been reported. Understanding the effect of the chelated metal is hindered because different chelators were used. The aim of this work was to investigate the effect of the nature of chelated cation on the photophysical and photodynamic properties of metalloporphyrins, using the same tetrapyrrole core as a chelator of Ag(II), Cu(II), Fe(III), In(III), Mn(III), or Zn(II). Results demonstrated that with the exception of Ag(II), all paramagnetic metalloporphyrins were inefficient as generators of singlet oxygen and did not act as PSs. In contrast, the coordination of diamagnetic ions produced highly efficient PSs. The unexpected photodynamic activity of the Ag(II)-containing porphyrin was attributed to reduction of the chelated Ag(II) to Ag(I) or to demetallation of the complex, caused by cellular reductants and/or by exposure to light. Our results indicate that in biological systems, where PSs localize to various organelles and are subjected to the action of enzymes, reactive metabolites, and reducing or oxidizing agents, their physicochemical and photosensitizing properties change. Consequently, the photophysical properties alone cannot predict the anticancer efficacy of a PS.","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":" ","pages":"487-499"},"PeriodicalIF":3.3,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138458964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0