源自胱氨酸的细胞外分泌半胱氨酸可调节 HepG2 细胞的氧化和亲电压力。

IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Free Radical Research Pub Date : 2024-05-01 Epub Date: 2024-05-11 DOI:10.1080/10715762.2024.2350524
Hanako Aoki, Yasuhiro Shinkai, Masahiro Akiyama, Satoshi Yamazaki, Motohiro Nishida, Yoshito Kumagai
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引用次数: 0

摘要

虽然已知半胱氨酸(CysSH)会输出到细胞外空间,但其生物学意义还不十分清楚。本研究利用稳定同位素标记的胱氨酸(CysSSCys)研究了细胞外 CysSH 的移动,胱氨酸被转运到细胞内并还原成 CysSH。将 HepG2 细胞暴露于 100 µM 稳定同位素标记的 CysSSCys 后 1 小时,细胞培养基中出现了 70 µM 标记的 CysSH。收集细胞介质并与过氧化氢(H2O2)或大气亲电物(如 1,2-萘醌、1,4-萘醌和 1,4-苯醌)一起培养后,细胞介质中的 CysSH 几乎被完全消耗。相反,在 HepG2 细胞暴露于 H2O2 长达 2 小时的过程中,细胞外的 CysSH 水平没有发生变化,这表明 CysSSCys/CysSH 在细胞系统中发生了氧化还原循环。在更换或不更换含有来自 HepG2 细胞的 CysSH 的细胞培养基的情况下进行的实验表明,培养基中的 CysSH 显著抑制了因暴露于 H2O2 和 1,2-萘醌而引起的细胞蛋白质氧化和亲电修饰。我们还研究了酶和/或抗氧化剂在细胞内将 CysSSCys 还原成 CysSH 的过程中的参与情况。这些结果提供了新的发现,即从 CysSSCys 派生的细胞外 CysSH 在氧化和亲电压力的调节中发挥作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Extracellularly secreted cysteine derived from cystine regulates oxidative and electrophilic stress in HepG2 cells.

While cysteine (CysSH) is known to be exported into the extracellular space, its biological significance is not well understood. The present study examined the movement of extracellular CysSH using stable isotope-labeled cystine (CysSSCys), which is transported into cells and reduced to CysSH. Exposure of HepG2 cells to 100 µM stable isotope-labeled CysSSCys resulted in 70 µM labeled CysSH in cell medium 1 h after CysSSCys exposure. When the cell medium was collected and incubated with either hydrogen peroxide (H2O2) or atmospheric electrophiles, such as 1,2-naphthoquinone, 1,4-naphthoquinone and 1,4-benzoquinone, CysSH in the cell medium was almost completely consumed. In contrast, extracellular levels of CysSH were unaltered during exposure of HepG2 cells to H2O2 for up to 2 h, suggesting redox cycling of CysSSCys/CysSH in the cell system. Experiments with and without changing cell medium containing CysSH from HepG2 cells revealed that oxidative and electrophilic modifications of cellular proteins, caused by exposure to H2O2 and 1,2-naphthoquinone, were significantly repressed by CysSH in the medium. We also examined participation of enzymes and/or antioxidants in intracellular reduction of CysSSCys to CysSH. These results provide new findings that extracellular CysSH derived from CysSSCys plays a role in the regulation of oxidative and electrophilic stress.

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来源期刊
Free Radical Research
Free Radical Research 生物-生化与分子生物学
CiteScore
6.70
自引率
0.00%
发文量
47
审稿时长
3 months
期刊介绍: Free Radical Research publishes high-quality research papers, hypotheses and reviews in free radicals and other reactive species in biological, clinical, environmental and other systems; redox signalling; antioxidants, including diet-derived antioxidants and other relevant aspects of human nutrition; and oxidative damage, mechanisms and measurement.
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