Food Bioscience最新文献

{"title":"Preparation, characterization, and solubility enhancement of octenyl succinate anhydride debranched starch/1-Octacosanol nanocomplex","authors":"Meiru Ji ,&nbsp;Baoxin Gu ,&nbsp;Junqing Han ,&nbsp;Lingjin Li ,&nbsp;Yahui Gao ,&nbsp;Li Cheng ,&nbsp;Zhengbiao Gu ,&nbsp;Yan Hong","doi":"10.1016/j.fbio.2025.106174","DOIUrl":"10.1016/j.fbio.2025.106174","url":null,"abstract":"<div><div>1-Octacosanol (1-Octa) is recognized worldwide as a functional anti-fatigue agent with additional anti-inflammatory and energy-boosting benefits. However, it has low bioavailability owing to the poor water solubility and instability in the gastrointestinal tract; this limits its use in food, medical, and health care products. To address this, we aimed to prepare a water-soluble octenyl succinate anhydride debranched starch (OSD)-Octa complex and investigate the effects of degree of substitution of OSD and reaction temperature on the drug loading, structure, and water solubility of the complex. The contact angle of free 1-Octa was 110.3°, which decreased to &lt;55° in the complex, and the solubility of 1-Octa in the complex ranged from 0.329 ± 0.008 to 1.532 ± 0.023 mg/mL, which indicated a notable increase in the aqueous solubility of 1-Octa. Furthermore, 1-Octa was found to penetrate the amylose helical cavity of OSD and form exohydrophilic and endohydrophobic nanoparticles with OSD through hydrophobic interaction. Finally, OSD-Octa effectively mitigated the degradation of 1-Octa under extreme pH conditions and in the presence of digestive enzymes compared to that of free 1-Octa. These findings suggest that the OSD system is an effective approach to enhancing the solubility and stability of hydrophobic functional factors.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106174"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A lateral flow assay based on aptamer for the detection of AFM1 in milk samples","authors":"Yiyao Wu ,&nbsp;Mingwei Qin ,&nbsp;Peifang Chen ,&nbsp;Yu Li ,&nbsp;Xiaodong Lu ,&nbsp;Anqi Sun ,&nbsp;Zhouping Wang ,&nbsp;Junsong Yang","doi":"10.1016/j.fbio.2025.106175","DOIUrl":"10.1016/j.fbio.2025.106175","url":null,"abstract":"<div><div>With the growth of the economy in recent years, the issue of food safety has gotten worse. Aflatoxin M₁ (AFM₁) is a highly toxic substance that poses a serious health risk and is widely distributed in milk and other dairy products. Biosensors are widely used in the field of food safety, but there is a lack of on-site methods to detect AFM₁. In this study, we propose for the first time an Apt-LFA based on AuNPs that can accurately detect AFM₁. The test strip's linear range was 0–500 ng/mL, and its detection limit was as low as 0.21 ng/mL. The test strip's recoveries in actual milk samples reached 92%–104.35% with excellent accuracy and stability. The results of this study have important practical application value, filling the gap of aptamer paper-based sensor technology in the field of AFM₁ detection and expanding the application scope of aptamers in AFM₁ detection. The test strips can be used for point-of-care testing (POCT). It is an important contribution to the development of food safety.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106175"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recyclable magnetic HNTs@MIPs-Based SERS sensors for selective, sensitive, and reliable detection of capsaicin for gutter oil discrimination","authors":"Junkui Li,&nbsp;Chen Li,&nbsp;Wang Guo,&nbsp;Yiqing Guo,&nbsp;Xiaobo Zou,&nbsp;Zongbao Sun","doi":"10.1016/j.fbio.2025.106179","DOIUrl":"10.1016/j.fbio.2025.106179","url":null,"abstract":"<div><div>Gutter oil, which poses a serious threat to human health and has generated widespread attention in recent years, is misused as cooking oil, creating a major food safety concern. Capsaicin has been identified as a promising indicator for the detection of gutter oil. Hence, in this study, we introduce a novel hybrid approach for capsaicin detection that combines magnetic molecular imprinting (MMIP) with surface-enhanced Raman scattering (SERS) technology to rapidly and accurately identify gutter oil. A magnetic molecularly imprinted polymer is developed with the incorporation of purified halloysite nanotubes, which provide a large specific surface area. The resulting product, magnetic halloysite molecularly imprinted polymers (MHNTs@MIPs), serves as a specific isolation material for the enrichment of capsaicin from complex matrices. The Ag NPs@Si (silver nanoparticles deposited on a silicon wafer surface) and Au NPs (gold nanoparticles), known for their excellent signal enhancement capabilities, work synergistically as the SERS substrate. Substantially enhanced SERS peaks are observed at 1173 cm⁻¹, indicating the potential of this method for capsaicin detection and gutter oil identification. A strong log-linear relationship between SERS intensity and the logarithm of capsaicin concentration is established across a wide range of 0.05–100 μM, with a detection limit of 67.7 nM. Furthermore, for both simulated and real gutter oil samples, capsaicin recovery rates of 95.5%–110.3% and 96.3%–104.9% were achieved using the SERS sensor, demonstrating good consistency with certified high-performance liquid chromatography (HPLC) results. Therefore, this SERS sensor can be served as efficient method for detecting capsaicin and monitoring oil quality.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106179"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combinatorial radiation-induced mutagenesis of Bacillus licheniformis and comparative whole genome sequencing to elucidate mutagenic effects on protease production","authors":"Jamila A. Tuly ,&nbsp;Haile Ma ,&nbsp;Hossain M. Zabed ,&nbsp;Xianghui Qi ,&nbsp;Muhammad Asif ,&nbsp;Zhang Xinyan ,&nbsp;Garba Betchem ,&nbsp;Quaisie Janet","doi":"10.1016/j.fbio.2025.106163","DOIUrl":"10.1016/j.fbio.2025.106163","url":null,"abstract":"<div><div>This study demonstrates that sequential UV, pulse, and laser treatments are effective for inducing mutations in membrane proteins and enhancing the protease activity of <em>Bacillus licheniformis.</em> Of the six different treatment combinations tested, the sequential application of Laser, Pulse, and UV (LPU-2) provided the highest lethality rate (96.44 ± 1.97%) and was selected as the optimal combination for subsequent mutagenesis. Through high-throughput screening of the mutant library, 25 strains exhibiting more than 30% relative enzyme activity were selected for second-step screening. Among the strains, LPU-2.1 exhibited the highest protease activity (57.90 ± 0.45 U/mL) in shaking flask fermentation and maximum hydrolysis zone (3.24 cm). Finally, the whole genome resequencing of LPU-2.1 revealed significant genetic alterations, including 20.3 bp insertions in gene ctg_04128, which encodes a membrane protein (MprF). Compared to the wild strain, LPU-2.1 demonstrated a significant increase in protease activity, which was 68%. The genome of LPU-2.1 encoded a total of 4470 proteins, with 3174 sequences (70.91%) being annotated in the GO database. Further analysis in the KEGG platform indicated the active biosynthesis of lysine via L-Lysyl-tRNA in LPU-2.1 produced by enzyme 6.1.16 involved in the aminoacyl-tRNA biosynthesis pathway. These results disclose that the combinatorial irradiation of <em>B. licheniformis</em> caused a modification in the ABC transporter and the two-component systems, which might have impacted the protease production of the mutant.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106163"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical profiles and mechanisms insights into Liuwei Dihuang-based formulas in diabetic nephropathy treatment: An integrated transcriptomics with in vitro experiments","authors":"Yanxia Liu ,&nbsp;Anlei Yuan ,&nbsp;Zewen Wang ,&nbsp;Shijie Bi ,&nbsp;Zhenzhen Xu ,&nbsp;Chaoqun Liu ,&nbsp;Bin Yu ,&nbsp;Jiaye Tian ,&nbsp;Liansheng Qiao ,&nbsp;Zhaozhou Lin ,&nbsp;Jun Jia ,&nbsp;Yanling Zhang","doi":"10.1016/j.fbio.2025.106170","DOIUrl":"10.1016/j.fbio.2025.106170","url":null,"abstract":"<div><div>Liuwei Dihuang formula(LWDH) is widely accepted as a remedy for tonifying kidney due to its medicine and food dual nature. LWDH and its various derivative prescriptions(JWDHs) with edible herbs were used in the clinical treatment of diabetic nephropathy(DN). Although, the mechanisms of LWDH being increasingly clarified, mechanisms of various JWDHs remained largely unexplored. The purpose of this study is to explore the mechanisms of Guifu Dihuang(GFDH), Qiju Dihuang(QJDH), and Zhibai Dihuang(ZBDH) formulas in treating DN. Transcriptomics, including GO, KEGG, and GSEA, was used to identify potential targets and pathways, RT-qPCR for further validation. UPLC-MS/MS was utilized to uncover chemical profiling of GFDH, QJDH and ZBDH. Molecular docking and molecular dynamics simulations were conducted to screen components and assess the stability of drug-target binding. As results, GFDH, QJDH and ZBDH exhibited multi-target and multi-pathway characteristics in treating DN. They collectively regulated the CDKN1A/FOXO signaling pathway, and QJDH uniquely modulated the PGK1/HIF-1 signaling pathway. UPLC-MS/MS identified 72 components across GFDH, QJDH and ZBDH, with 45 common and 7 unique to QJDH. Molecular docking indicated that unique components in QJDH, including neochlorogenic acid, chlorogenic acid, naringenin, kaempferide, and oroxylin A, are potential agents for intervening DN through <em>PGK1</em>. Molecular dynamics simulation confirmed that neochlorogenic acid exhibited stable binding with <em>PGK1</em>, further supporting the validation of the unique mechanism of action of QJDH. This study highlights the diverse mechanisms of GFDH, QJDH, and ZBDH in combating DN, offering a theoretical basis for clinical use and a foundation for developing food therapy approaches.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106170"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative analyzing the molecular basis of Clerodendranthus spicatus aqueous extract in hyperuricemia nephropathy via network pharmacology and experimental validation","authors":"Chuang Li ,&nbsp;Danyao Lin ,&nbsp;Jianting Ouyang ,&nbsp;Junyi Liu ,&nbsp;Shouhai Wu ,&nbsp;Delun Li ,&nbsp;Fan Yang ,&nbsp;Haohao Quan ,&nbsp;Yimeng Li ,&nbsp;Qiaoru Wu ,&nbsp;Guirui Huang ,&nbsp;Wei Mao","doi":"10.1016/j.fbio.2025.106152","DOIUrl":"10.1016/j.fbio.2025.106152","url":null,"abstract":"<div><div>Clerodendranthus spicatus (CS) is recognized as a beneficial beverage for the management of hyperuricemia and urinary system irritation. However, the protective mechanisms of CS against hyperuricemia nephropathy (HN) remain insufficiently understood. This study aims to explore the molecular basis of CS against HN and to validate these findings <em>in vivo</em>. Using a rat model of HN, CS demonstrated anti-inflammatory and fibrotic mitigation effects, thereby preserving kidney function and improving the tubular pathological phenotypes associated with HN. Results from Liquid Chromatography Tandem Mass Spectrometry (UPLC-LC-MS/MS) analysis identified seven phenolic compounds from CS, including caffeic acid (CAA), danshensu (DSS), 3, 4-dihydroxybenzaldehyde (protocatechualdehyde, PCA), rosmarinic acid (RA), cichoric acid (CA), lithospermic acid (LA), and salvianolic acid B (Sal B). Network pharmacology analysis identified nine critical targets, including SRC, MAPK1, MAPK3, PIK3R1, PIK3CA, HRAS, EP300, GRB2, and RELA, that were predominantly enriched in pathways related to inflammation, fibrosis and cell cycle regulation. Molecular docking models demonstrated strong binding affinity between the critical targets and the phenolic compounds from CS. In the <em>in vivo</em> study, increased phosphorylation of MAPK, PI3K, and AKT were observed in the HN group. However, CS intervention resulted in their dephosphorylation. Elevated levels of EP300 were present in the dilated renal tubular epithelial cells of HN group, while its expression decreased following CS intervention. The HN group also exhibited decreased FOXO3 expression in renal tubule lesions, while CS restored FOXO3 expression. This study elucidates that multiple components of CS interact with the critical targets within the FOXO pathway, contributing to its therapeutic effects on HN-related pathological process.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106152"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of heat pretreatment and transglutaminase treatment on the gelling properties of pea protein","authors":"Suqi Yang ,&nbsp;Liling Tao ,&nbsp;Zihang Yan ,&nbsp;Kefan Ouyang ,&nbsp;Qin Zhang ,&nbsp;Yuanyuan Feng ,&nbsp;Qiang Zhao","doi":"10.1016/j.fbio.2025.106166","DOIUrl":"10.1016/j.fbio.2025.106166","url":null,"abstract":"<div><div>Pea proteins are widely used in the food industry due to their good gelling properties, and their gelling ability depends not only on the protein concentration but also on the coagulant and gelation conditions. In this study, we investigated the mutual cross-linking effect of pea proteins under three different preheating temperatures (60 °C, 80 °C, and 100 °C) and without preheating treatment, which was induced by different transglutaminase (TGase) additions (0%, 3%, 4%, and 5%). It has been found that both TGase and heating treatments can enhance the gelling ability of proteins, based on which, their combined effects on the gelling ability of pea proteins as an interesting modification strategy remains to be validated. The optimized results indicated that the mechanical strength of pea protein gel reached its peak when subjected to heating at 60 °C with the addition of 4% TGase, demonstrating superior gelling and water-holding capacity compared to the control group. Different temperature treatments and the addition of different levels of TGase improved the three-dimensional network structure of the pea protein gel thereby affecting the water presence state. This result opens up new possibilities and is of great significance for the application of TGase-induced pea protein gels in the food industry.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106166"},"PeriodicalIF":4.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A blockchain and IPFS-based system for monitoring the geographical authenticity of Codonopsis pilosula","authors":"Yazhi Yang ,&nbsp;Yanbo Song ,&nbsp;Yongpeng Duan ,&nbsp;Xuan Wang ,&nbsp;Xiaosa Duan ,&nbsp;Wenjuan Lu ,&nbsp;Qiqi Guo ,&nbsp;Zhenyu Liu","doi":"10.1016/j.fbio.2025.106091","DOIUrl":"10.1016/j.fbio.2025.106091","url":null,"abstract":"<div><div>The quality of Geo-authentic herbs is determined by unique growth conditions, such as soil composition and climate, which result in higher concentrations of trace elements, minerals, and secondary metabolites compared to common herbs. However, existing quality monitoring methods for authentic medicinal materials face issues such as limited monitoring dimensions, high data storage pressure, and insufficient traceability efficiency. To address these issues, this study proposes a novel Geo-authentic herbs quality monitoring method based on a “blockchain + InterPlanetary File System (IPFS)” architecture. This approach employs a distributed storage framework where large-scale data, including climate and soil conditions, are stored in IPFS, while blockchain records corresponding hash values, achieving authenticity verification while reducing on-chain storage pressure. To validate the effectiveness of the proposed method, we constructed a set of quality monitoring systems for Lu Codonopsis pilosula in Lingchuan County, Shanxi Province, China, covering the entire lifecycle from sowing to commercialization. Performance testing demonstrated that compared to traditional blockchain systems, the proposed system reduced node synchronization latency by approximately 37.51%, data growth rates by 50.82%, and storage space requirements by 49.82%. This study represents the first application of the “blockchain + IPFS” approach to Geo-authentic herbs quality monitoring, addressing gaps that traditional methods fail to cover. It provides a scalable solution for quality assurance and traceability in the authentic medicinal materials supply chain, offering a technological framework that supports sustainable development in the food and pharmaceutical industries.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106091"},"PeriodicalIF":4.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review on the neuropharmacological effects of Phoenix dactylifera and the potential role of its phenolic constituents","authors":"Md Mohaiminul Islam ,&nbsp;Razina Rouf ,&nbsp;Jamil A. Shilpi ,&nbsp;Bassem Yousef Sheikh ,&nbsp;Mohammad S. Mubarak ,&nbsp;Shaikh Jamal Uddin","doi":"10.1016/j.fbio.2025.106141","DOIUrl":"10.1016/j.fbio.2025.106141","url":null,"abstract":"<div><div>Nutraceuticals and natural products are increasingly popular for their therapeutic potential and long-standing role in traditional medicine<em>. Phoenix dactylifera</em>, or the date palm, is recognized for its nutritional and medicinal benefits and is gaining attention for its potential in neuropharmacological applications. The date palm's neuroprotective potential is linked to its high phenolic content, which supports central nervous system (CNS) health and possibly prevents neurodegenerative diseases. Date palm extracts modulate neurotransmitters like acetylcholine, dopamine, and GABA, enhancing memory, cognitive function, and pain relief. Additionally, the plant exhibits aphrodisiac properties and may reduce anxiety, shorten labor, and ease labor pain during pregnancy. Date palm phenolics combat oxidative stress and inhibit key inflammatory pathways by reducing pro-inflammatory cytokines like NF-κB, TNF-α, and IL-1β. It also suppresses the synthesis of eicosanoids, cyclooxygenase-2 (COX-2), and nitric oxide synthase (NOS), all involved in inflammation. However, challenges such as poor bioavailability, enzymatic degradation, and limited blood-brain barrier (BBB) permeability hinder their therapeutic potential. Advanced encapsulation technologies, including liposomes, nanoemulsions, and polymeric micelles, offer effective solutions by protecting phenolics, enhancing bioavailability, and enabling targeted delivery to the brain, amplifying their efficacy. The phenolic content of date palms varies across cultivars, influencing their therapeutic properties and highlighting the importance of cultivar-specific profiling for targeted applications. This review explores the neuropharmacological effects of <em>Phoenix dactylifera</em>, emphasizing its phenolic compounds' therapeutic potential and the role of encapsulation technologies in overcoming key bioavailability and delivery challenges for CNS health management.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106141"},"PeriodicalIF":4.8,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic insights into stress responses and adhesion markers of co-encapsulated Lactobacillus reuteri DPC16 with Cyclocarya paliurus leaf extracts during in vitro gastrointestinal tract simulation (GITS)","authors":"Li Ying Jessie Lau ,&nbsp;Siew Young Quek","doi":"10.1016/j.fbio.2025.106148","DOIUrl":"10.1016/j.fbio.2025.106148","url":null,"abstract":"<div><div>This study explores the mucin-adhesive properties and the proteomic changes in <em>L. reuteri</em> DPC16 co-encapsulated with <em>Cyclocarya paliurus</em> (CP) leaf extracts by hempseed protein isolate. The mucin adhesion ability of <em>L. reuteri</em> DPC16 was assessed and visualized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Novel microcapsules containing <em>L. reuteri</em> DPC16 and CP leaf extracts were subjected to sequential simulated gastrointestinal conditions, including gastric, duodenal, and ileal phases, with corresponding pH adjustments and digestive enzymes. Surface proteins from <em>L. reuteri</em> DPC16 were extracted at each stage, separated by SDS-PAGE, and analyzed using LC-ESI-MS/MS to identify and quantify differentially expressed proteins. Over 200 surface proteins were identified, with significant upregulation of stress-responsive proteins such as heat shock proteins and enzymes involved in cell wall synthesis. These, along with proteins associated with metabolic pathways, contributed to the stability and activity of the probiotic under gastrointestinal conditions. CP leaf extracts further enhanced oxidative stress resistance and modulated protein expression to boost probiotic efficacy. Notably, increased expression of mucosal adhesion-related proteins, including mucus-binding and moonlighting-binding proteins, suggested an enhanced ability of <em>L. reuteri</em> DPC16 to colonize the gut. Proteomic analysis provided comprehensive insights into the molecular dynamics of <em>L. reuteri</em> DPC16, uncovering its adaptive mechanisms and functional potential in diverse environments. These findings highlight the value of protein markers in validating the health-promoting properties of <em>L. reuteri</em> DPC16, supporting its application in functional foods and therapeutic supplements.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106148"},"PeriodicalIF":4.8,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143479852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}