Food Bioscience最新文献

{"title":"A new nanosurface covalent organic framework probe for gold nanosol RRS/Abs detection of trace ammonium perfluorooctanoate","authors":"Chenguang Yi ,&nbsp;Aihui Liang ,&nbsp;Guiqing Wen ,&nbsp;Zhiliang Jiang","doi":"10.1016/j.fbio.2025.106162","DOIUrl":"10.1016/j.fbio.2025.106162","url":null,"abstract":"<div><div>Ammonium perfluorooctanoate (PFOA) is an important material, such as for manufacturing of non-stick cookware. It can pose a threat to human health. Developing a highly sensitive and selective sensing platform for PFOA has become a hot topic in the fields of ecological environment and human health. This study was synthesized a new recognition and nanocatalytic amplification difunctional nanopolyvinyl chloride-loaded covalent organic framework nanosurface molecularly imprinted probe (PVC@COF) by solvothermal procedure. It was found that PVC@COF strongly catalyzed the new nanogold indicator reaction of sodium formate-chlorauric acid. The generated gold nanoparticles (AuNP) showed intense resonance Rayleigh scattering (RRS) and surface plasmon resonance absorption (Abs) effects. When PFOA was added, the probes specifically recognized PFOA to form PVC@COF-PFOA conjugates with greater catalytic activity, more AuNP were generated and the RRS and Abs signals amplified linearly, thus realizing rapid, sensitive and selective quantitative analysis of PFOA. The linear detection ranges of RRS and Abs were 0.0025–0.05 and 0.002–0.05 μmol/L, and the detection limits were 1.09 and 1.43 nmol/L PFOA respectively. This work provides a new COF probe-RRS detection strategy for trace PFOA, and has certain application potential in food safety and environmental protection.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106162"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical and chemical properties and anti-apoptotic effects on C2C12 cells of hydrolyzed tuna (Katsuwonus pelamis) blood powder","authors":"Mantira Hawangjoo ,&nbsp;Panlekha Rungruang ,&nbsp;Morakot Sroyraya ,&nbsp;Pennapa Chonpathompikunlert ,&nbsp;Sittiruk Roytrakul ,&nbsp;Wanida Sukketsiri ,&nbsp;Wanwimol Klaypradit","doi":"10.1016/j.fbio.2025.106168","DOIUrl":"10.1016/j.fbio.2025.106168","url":null,"abstract":"<div><div>This study aimed to develop hydrolyzed tuna blood powders (HTBP) with potential anti-apoptotic and muscle recovery properties. HTBP was produced alcalase, trypsin, and pepsin at concentrations of 0.5%, 1.0%, and 1.5%. The physical and chemical properties of HTBP were evaluated. The results showed no significant difference in yield (<em>p</em> &gt; 0.05), with a range of 7.08% ± 0.78% to 9.26 % ± 1.74%. HTBP produced with alcalase at all concentrations yielded a higher amount of protein (76.86% ± 0.5%–79.1% ± 1.10%) compared to trypsin and pepsin. Glutamic acid, cysteine, and aspartic acid were identified as the main amino acids in HTBP. The water solubility index of all HTBP was high (&gt;90%). Antioxidant values increased with higher protein content. Hydrolyzed tuna blood with 0.5% alcalase (HTBPA) and 0.5% trypsin were selected for further investigation of their biological activities, assessed using C2C12 muscle cells under oxidative stress conditions induced by hydrogen peroxide (H₂O₂). In H₂O₂-induced C2C12 cells, HTBPA and HTBPT reduced nitric oxide (NO) secretion and intracellular reactive oxygen species (ROS) levels. Furthermore, HTBPA and HTBPT significantly decreased the percentage of apoptotic cells in these H₂O₂-induced C2C12 cells. They also inhibited H₂O₂-induced apoptosis by modulating apoptosis-related proteins, including NF-κB, Bax, and caspase-3. Additionally, HTBPA and HTBPT suppressed the phosphorylation levels of key signaling proteins such as Akt, ERK1/2, and p38, thereby contributing to enhanced cell survival. These findings suggest that hydrolyzing tuna blood, waste of the canned tuna industry, can create a valuable ingredient for promoting muscle health.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106168"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced ergothioneine production in Pleurotus tuber-regium through submerged fermentation optimization and selenium-driven mechanistic insights","authors":"Lei Chen ,&nbsp;Zhuomin Zhang ,&nbsp;Zihan Guan ,&nbsp;Jing Sun ,&nbsp;Yanhui Sun ,&nbsp;Mengmeng Xu ,&nbsp;Ka-Hing Wong ,&nbsp;Guiyang Shi ,&nbsp;Zhongyang Ding","doi":"10.1016/j.fbio.2025.106146","DOIUrl":"10.1016/j.fbio.2025.106146","url":null,"abstract":"<div><div>Ergothioneine (EGT) production in <em>Pleurotus tuber-regium</em> was significantly enhanced through Na₂SeO₃-induced oxidative stress, in combination with optimized carbon sources and precursor amino acids. Initially, glucose and rhamnose (20 g/L each) as dual carbon sources increased EGT synthesis. Supplementation with 0.75 mM cysteine and 1 mM methionine further promoted EGT biosynthesis by providing essential precursors. Additionally, the introduction of 0.3 mM Na₂SeO₃ induced oxidative stress, leading to an EGT accumulation of 4.68 mg/g dry weight (DW) in the mycelium and a yield of 35.27 mg/L, representing a 4.25-fold and 162% increases compared to the control, respectively. RNA-Seq analysis revealed that selenium exposure triggered the upregulation of genes involved in antioxidant defense, reinforcing redox homeostasis and driving EGT accumulation. This study highlights the mechanistic role of selenium in modulating antioxidant pathways, offering a promising strategy for enhancing EGT production in the nutraceutical and biotechnological fields.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106146"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of collagen peptide-calcium chelate and its promotion of calcium absorption in vitro and in vivo","authors":"Yudong Wang ,&nbsp;Meiyi Chen ,&nbsp;Yuyu Liu ,&nbsp;Yang Hu ,&nbsp;Juan You ,&nbsp;Xiaobo Hu","doi":"10.1016/j.fbio.2025.106150","DOIUrl":"10.1016/j.fbio.2025.106150","url":null,"abstract":"<div><div>The aim of this study was to investigate the structure and stability of silver carp skin collagen peptide-calcium chelate (SSCPs-Ca) and evaluate its effectiveness in promoting calcium absorption. SSCPs were prepared by two-step enzymatic hydrolysis and chelated with calcium to form SSCPs-Ca. The structure of SSCPs-Ca was characterized by ultra-violet spectroscopy, fluorescence spectroscopy, particle size distribution, scanning electron microscopy and atomic force microscopy. Then explored the effects of temperature, lactose, sodium chloride and phosphate on its stability. The calcium uptake of SSCPs-Ca was evaluated using Caco-2 cell and zebrafish osteoporosis models. Compared to SSCPs, SSCPs-Ca exhibited notable improvements in particle size and adopted a stable, osteocalcin-like crystal structure. SSCPs-Ca exhibited enhanced electrostatic repulsion and thermal stability. SSCPs-Ca significantly enhanced calcium transport and absorption at the cellular level, outperforming CaCl₂. Unlike traditional supplements, its efficiency remained unaffected by dietary components such as lactose and NaCl. SSCPs-Ca operated independently of Vitamin D for calcium transport, emphasizing its innovative mechanism. SSCPs-Ca at a concentration of 125 μg/mL demonstrated superior efficacy in mitigating osteoporosis in zebrafish models, achieving comparable results to etidronate disodium at a concentration of 300 μg/mL. These findings established SSCPs-Ca as a groundbreaking calcium supplement with the potential to significantly improve bone health. By addressing calcium deficiency and associated risks with enhanced bioavailability and stability, SSCPs-Ca offered a promising alternative to traditional calcium supplements, paving the way for innovative strategies in managing calcium-related health challenges.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106150"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143479944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral administration of alpha-ketoglutarate partially ameliorates Cadmium-compromised BMSC mobilization and cranial bone repair","authors":"Yuna Ji ,&nbsp;Pengchao Zheng ,&nbsp;Xinchen Wang ,&nbsp;Renjie Hu ,&nbsp;Huigen Luo ,&nbsp;Baoshan Xu","doi":"10.1016/j.fbio.2025.106169","DOIUrl":"10.1016/j.fbio.2025.106169","url":null,"abstract":"<div><div>Cadmium (Cd) is a widespread environmental heavy metal pollutant known to disrupt bone metabolism, causing significant health concerns. Cd exposure impairs osteogenesis in primary bone marrow-derived mesenchymal stem cells (BMSCs) through mechanisms involving oxidative stress and NF-κB inflammation signaling. However, the underlying effects and mechanisms by which Cd hampers bone defect repair remain largely unknown. Additionally, alpha-ketoglutarate (AKG) is a dietary supplement as an antioxidant to potentially improve healthy longevity. In this study, we used a rat model to investigate the toxicology mechanisms of Cd to suppress bone repair and identify orally administrated AKG as a potential therapeutic intervention. Herein, we demonstrated that Cd inhibited BMSC and vascular endothelial cell motility, markedly reducing lamellipodia formation and cell migration. Cd also disrupted cytoskeletal reorganization and cell adhesion in BMSCs. Moreover, our data indicated that Cd induced abnormal expression of RhoA protein and suppressed the Notch1 pathway. <em>In vivo</em> studies further revealed that Cd significantly impaired cranial bone defect repair, tissue healing, and the formation of new vascular structures. These findings suggested that Cd interfered with BMSC migration and homing to bone defect sites, obstructing bone repair and healing. Intriguingly, AKG partially restored Cd-impaired BMSC mobilization and exhibited partial efficacy in ameliorating cranial bone defect repair compromised by Cd. Our study uncovered a novel mechanism by which Cd exposure impeded bone repair and presented AKG administration as a potential approach to mitigate Cd-induced bone tissue damage. This work may broaden our current understanding of Cd-induced bone disorders.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106169"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143479943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the efficacy of modified plant vaccine antigens in animal immunization: A systematic review","authors":"Tatyane Martins Cirilo ,&nbsp;Ana Laura Grossi de Oliveira ,&nbsp;Jordânia Costa Pinto ,&nbsp;José Bryan da Rocha Rihs ,&nbsp;Ana Cristina Loiola Ruas ,&nbsp;Williane Fernanda Siqueira ,&nbsp;Joseane Camilla de Castro ,&nbsp;Nathalia Sernizon Guimarães ,&nbsp;Ramayana Morais de Medeiros Brito ,&nbsp;Lilian Lacerda Bueno ,&nbsp;Thomas J. Cleij ,&nbsp;Joni Esrom Lima ,&nbsp;Ricardo Toshio Fujiwara","doi":"10.1016/j.fbio.2025.106178","DOIUrl":"10.1016/j.fbio.2025.106178","url":null,"abstract":"<div><h3>Background</h3><div>Edible vaccines produced in genetically modified plants represent a novel approach to immunization, to express antigens capable of eliciting immune responses at mucosal surfaces. This method provides a potential cost-effective solution for vaccine delivery, particularly beneficial in resource-limited settings.</div></div><div><h3>Aim</h3><div>This review aims to assess the efficacy of edible vaccines derived from transgenic plants in eliciting immune responses in animal models against various infectious and parasitic diseases.</div></div><div><h3>Methods</h3><div>A comprehensive search of PubMed, Embase, and Scopus up to June 2024 identified 138 references. Following Cochrane guidelines, PRISMA standards, and the CAMARADES risk of bias checklist, 16 studies met the inclusion criteria and focused on edible vaccines for animal immunization.</div></div><div><h3>Results</h3><div>The findings indicate that edible vaccines can effectively elicit both humoral and mucosal immune responses in animals. For instance, transgenic plants expressing antigens for hepatitis B and human papillomavirus demonstrated high efficacy, with significant increases in antigen-specific antibody levels post-immunization. Studies on bacterial diseases using LT-B antigens from <em>Escherichia coli</em> expressed in corn showed robust, sustained antibody responses. Additionally, for parasitic diseases, transgenic rice expressing <em>Ascaris</em> antigen reduced larval burden, indicating protective efficacy. However, variations in immune response depending on plant type and antigen stability highlight the need for further optimization to achieve consistent efficacy across different diseases.</div></div><div><h3>Conclusion</h3><div>Edible vaccines from antigen-expressing plants show promise as a viable alternative to traditional methods, with strong immunogenicity in animal models. Further research is needed to address variability and optimize production for human use, potentially improving vaccine access in underserved regions.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106178"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A CYP450 monooxygenase MaCYP82C169 discovered from mulberry leaves catalyzes the methyl oxidation reaction in 1-deoxynojirimycin biosynthesis","authors":"Jiahe Fan ,&nbsp;Yangzhen Liao ,&nbsp;Yaqin Zhao ,&nbsp;Jingqiong Wan ,&nbsp;Yuan Wei ,&nbsp;Zhen Ouyang","doi":"10.1016/j.fbio.2025.106177","DOIUrl":"10.1016/j.fbio.2025.106177","url":null,"abstract":"<div><div>1-Deoxynojirimycin (DNJ), the primary active compound in mulberry leaves, is a potent α-glucosidase inhibitor with notable hypoglycaemic properties. As a polyhydroxypiperidine alkaloid, DNJ contains four hydroxyl groups (3-OH, 4-OH, 5-OH, and 7-OH) in its structure. The upstream biosynthetic pathway of DNJ from lysine to 2-methylpiperidine was previously elucidated, and the first hydroxylase and its hydroxylation site (4-OH) in the downstream pathway were excavated. However, the enzymes involved in hydroxylating other positions remain undefined. Based on the transcriptome data of mulberry leaves, this work identified another CYP450 enzyme involved in the hydroxylation process of DNJ biosynthesis pathway. Yeast whole-cell biocatalytic assays and <em>in vitro</em> enzymatic reactions demonstrated that MaCYP82C169 regioselectively hydroxylates the C-7 position of (<em>R</em>)-2-methylpiperidine, producing (<em>S</em>)-2-hydroxymethylpiperidine. Overexpression and gene-silencing experiments further validated MaCYP82C169's regulatory role in DNJ biosynthesis. Phylogenetic analysis classified MaCYP82C169 within the CYP82C subfamily. Furthermore, molecular docking and site-directed mutagenesis experiments revealed that the G389 residue of MaCYP82C169 is critical for its catalytic activity of (<em>R</em>)-2-methylpiperidine. This study advances the understanding of DNJ biosynthesis by identifying key hydroxylase and confirming the sequence of methylation followed by hydroxylation. These findings provide a foundation for completing the analysis of the DNJ biosynthetic pathway.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106177"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the stability and antimicrobial activity of emulsions loaded with thymol for the post-harvest sanitization of lettuce (Lactuca sativa L)","authors":"Daniel Lucino Silva dos Santos ,&nbsp;Jeniffer Ferreira de Miranda ,&nbsp;Rodney Alexandre Ferreira Rodrigues ,&nbsp;Ana Silvia Prata ,&nbsp;Nathália Cristina Cirone Silva","doi":"10.1016/j.fbio.2025.106176","DOIUrl":"10.1016/j.fbio.2025.106176","url":null,"abstract":"<div><div>Thymol is a monoterpene with broad antimicrobial activity and great potential for use in applications against pathogens and spoiling agents, as it causes changes in the permeability of microorganism cell walls. However, its low solubility in water can alter its bioavailability and antimicrobial effectiveness. The aim of this study was to evaluate the physicochemical and antimicrobial stability of emulsions made up of thymol (standard reference substance SRS-thymol) and <em>Lippia sidoides</em> essential oil obtained by primary emulsification and high energy shear methods and stored at temperatures of 5 and 25 °C for 30 days. The most stable emulsions were obtained by the high-energy method and showed a range of −28.26 to −24.63 mV for Zeta potential (ZP); 0.22 to 0.41 for dispersion index (PDI) and drop sizes of 145.46–193.40 nm. The treatment with the lowest Minimum Inhibitory Concentration (MIC) values was against the <em>Staphylococcus aureus</em> cocktail, being 0.50–1 mg/mL and 1–2 mg/mL for the SRS-thymol and <em>L. sidoides</em> essential oil emulsions, respectively. The application of the emulsions showed reductions of up to 3.5 logs of CFU/mL in lettuce samples by the challenge test with <em>Salmonella</em> Typhimurium, <em>S. aureus</em> and <em>Escherichia coli</em>. However, treatments with higher concentrations (2 × MIC) cause side effects related to changes in the texture and color of the leaves after 15 min of sanitization.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106176"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of fermented feed on volatile flavor of duck meat through fatty acid metabolism pathways in gut microbiota and muscle","authors":"Ligen Xu ,&nbsp;Tingting Mao ,&nbsp;Chunqing Jiang ,&nbsp;Zhaoxia Cai ,&nbsp;Tao Zeng ,&nbsp;Yong Tian ,&nbsp;Weifeng Feng ,&nbsp;Lizhi Lu ,&nbsp;Wenwu Xu","doi":"10.1016/j.fbio.2025.106186","DOIUrl":"10.1016/j.fbio.2025.106186","url":null,"abstract":"<div><div>Fermented feed (FF) effectively safeguards animal health as an antibiotic alternative. Despite its growing use, research exploring its impact on the volatile flavor compounds of duck meat remains limited. To address this gap, this study aimed to uncover the fundamental mechanisms underlying FF's influence on these compounds through multi-omics technologies. FF led to significant differences in volatile flavor compounds and free fatty acids (FFAs) between groups. Precursor substances of five key flavor compounds were associated with these differential FFAs, with both cecal microbiota and muscle tissue sequencing highlighting lipid metabolism pathways. In the differential lipid metabolism pathways, key genes were identified that are involved in the regulation of intramuscular fat deposition and fatty acid composition. This research marks a crucial step in assessing antibiotic alternatives' impact on the meat flavor.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106186"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mixed fermentation of citrus peel pomace with Trichoderma koningii, Aspergillus oryzae and Lactobacillus casei: Process optimization, antioxidant activities and non-targeted metabolomics analysis","authors":"Hui Yu ,&nbsp;Xian Xu ,&nbsp;Junxiong Hao ,&nbsp;Xiaoxia Zuo ,&nbsp;Jing Wang ,&nbsp;Liqin Zhu ,&nbsp;Ming Chen ,&nbsp;Yongmei Lyu ,&nbsp;Zhihong Yan ,&nbsp;Yonggen Shen ,&nbsp;Zhipeng Cai","doi":"10.1016/j.fbio.2025.106180","DOIUrl":"10.1016/j.fbio.2025.106180","url":null,"abstract":"<div><div>Citrus peel pomace is a waste product after industrial processing, but it is rich in various active ingredients. In this study, a mixture of <em>Trichoderma koningii</em>, <em>Aspergillus oryzae</em> and <em>Lactobacillus casei</em> was used for the fermentation of navel orange peel pomace. The effects of strain ratio, inoculum amount, and fermentation temperature on the total phenolic content (TPC) of navel orange peel pomace were investigated via single-factor test and orthogonal test, and the best process was obtained. The antioxidant activity and non-targeted metabolomics analysis of orange peel pomace by mixed fermentation were performed. The optimal process for mixed fermentation of navel orange peel pomace was identified as follows: inoculation ratio of <em>Trichoderma koningii</em>, <em>Aspergillus oryzae</em>, and <em>Lactobacillus casei</em> was 1:5:7, inoculum amount was 6%, and fermentation temperature was 30 °C. Compared to unfermented navel orange peel pomace, mixed fermentation significantly increased the TPC and antioxidant capacity of navel orange peel pomace, especially in ABTS and FRAP. The non-targeted metabolomics identified 458 differential metabolites, including phenols, flavonoids, terpenoids, alkaloids, phenylpropanoids analogs. Pathway analysis was also performed, and 35 key pathways were identified. The correlation analysis showed that a number of metabolites, such as pinoresinol, gentisic acid, quercetin 3-galactoside 7-rhamnoside, stearidonic acid, quercetin 3-lathyroside, (r) -carvone, were strongly and positively correlated with TPC and antioxidant capacity. To sum up, mixed fermentation could improve the TPC and antioxidant capacity of navel orange peel pomace.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106180"},"PeriodicalIF":4.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}