Food BiosciencePub Date : 2026-04-01Epub Date: 2026-02-09DOI: 10.1016/j.fbio.2026.108445
Yutong Hu , Ying Wang , Xiaohong Sun , Li Wang , Jiang Jiang
{"title":"Structural modification of mung bean protein isolate via heat–pH shifting and limited enzymatic hydrolysis: Enhancing gelation of additive-free yogurt","authors":"Yutong Hu , Ying Wang , Xiaohong Sun , Li Wang , Jiang Jiang","doi":"10.1016/j.fbio.2026.108445","DOIUrl":"10.1016/j.fbio.2026.108445","url":null,"abstract":"<div><div>Legume protein–based yogurts formulated without added fat or stabilizers typically exhibit poor gelation capacity and are prone to phase separation. To overcome this limitation and extend the applicability of pH-shifting in yogurt systems, a synergistic modification strategy for mung bean protein isolate (MBPI) was developed by integrating heat–alkali treatment (pH 10.5, 50 °C) with limited proteolysis using papain or bromelain. SDS–PAGE analysis showed that papain induced controlled hydrolysis while largely preserving the medium-molecular-weight protein fractions. Compared with native MBPI (solubility: 23.7 %), MBPI subjected to pH-shifting combined with papain treatment exhibited a pronounced increase in solubility (75.5 %) and formed gels with enhanced water-holding capacity (WHC, 78.6 %) and improved viscoelastic properties (G′/G″). The combined treatment slightly reduced gel hardness due to particle size reduction, resulting in a finer gel structure suitable for yogurt products. LF-NMR analysis further confirmed a more restricted water distribution within the modified gels, indicating the formation of a stable and cohesive gel network. Overall, these findings demonstrate an effective approach for alleviating textural limitations in plant-based yogurts and support the development of high-quality, clean-label fermented products.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"78 ","pages":"Article 108445"},"PeriodicalIF":5.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146161925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food BiosciencePub Date : 2026-04-01Epub Date: 2026-02-10DOI: 10.1016/j.fbio.2026.108462
YaXin Gu , Qingqiu Ye , Xin Feng , Junzhu Li , Zichen He , Ming Zhang , Yanling Hao , Zhengyuan Zhai
{"title":"Comparative genomics reveals that Bifidobacterium longum subsp. longum BZG4 alleviates constipation in mice via synergistic xylooligosaccharide","authors":"YaXin Gu , Qingqiu Ye , Xin Feng , Junzhu Li , Zichen He , Ming Zhang , Yanling Hao , Zhengyuan Zhai","doi":"10.1016/j.fbio.2026.108462","DOIUrl":"10.1016/j.fbio.2026.108462","url":null,"abstract":"<div><div>Constipation is a common gastrointestinal disorder that affects all age groups. Probiotics show efficacy in relieving constipation; however, their efficacy is highly strain-specific, and the genetic basis for this heterogeneity remains unclear. This study investigated the genetic determinants and underlying mechanisms of different <em>Bifidobacterium longum</em> subsp. <em>longum</em> strains in alleviating constipation. Among the three strains evaluated in loperamide-induced constipation mice, only BZG4 significantly alleviated constipation. Comparative genomic analysis revealed that a unique xylooligosaccharide (XOS) utilization cluster was only located in the chromosome in strain BZG4, which was further validated by its ability to grow with XOS as the sole carbon source. Synbiotic supplementation with BZG4 and XOS produced synergistic effects, evidenced by significant improvements in fecal water content, 5-h fecal pellet output, whole-gut transit time, and small intestinal propulsion rate. Mechanistically, the synbiotic BZG4-XOS alleviated constipation by modulating gut microbiota to increase the butyric acid level in feces, thereby restoring the integrity of the enteric nervous system, regulating the relevant neuromodulators and enhancing MUC2-dominated mucus and water secretion. These findings determined the strain-specific XOS cluster of BZG4 as a genetic basis for constipation relief and provided a genetic strategy for developing precision synbiotics targeting gastrointestinal motility disorders.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"78 ","pages":"Article 108462"},"PeriodicalIF":5.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146162046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Targeted metabolomics reveals that phosphorylated Cyclocarya paliurus polysaccharides prevent dextran sulfate sodium-induced ulcerative colitis via modulating eicosanoid metabolism","authors":"Haibin Zhu , Xiangwen Pan , Mingyue Shen , Xianxiang Chen , Haiyan Zhao , Jianhua Xie","doi":"10.1016/j.fbio.2026.108460","DOIUrl":"10.1016/j.fbio.2026.108460","url":null,"abstract":"<div><div>Ulcerative colitis (UC) is a chronic inflammatory disease characterized by intestinal tract inflammation and mucosal barrier damage. Previous studies have demonstrated that phosphorylated <em>Cyclocarya paliurus</em> polysaccharide (P-CPs) can prevent UC symptoms, but the molecular mechanism involving eicosanoid metabolism in dextran sulfate sodium (DSS) -induced murine colitis remains unclear. P-CPs with varying substitution degrees (PL, PM, and PH; Degrees of substitution = 0.04, 0.10, and 0.18, respectively) were administered before DSS induction to analyze their preventive effects on UC and the mechanism of eicosanoid metabolism based on targeted metabolomics. The results revealed that P-CPs significantly suppressed the expression of pro-inflammatory cytokines interleukin-1β (IL-1β) and interferon-γ (IFN-γ) and promoted the secretion of anti-inflammatory cytokine interleukin-2 (IL-2). Metabolomic analysis showed that the production of pro-inflammatory mediators such as thromboxane B<sub>2</sub> (TXB<sub>2</sub>), prostaglandin D<sub>2</sub> (PGD<sub>2</sub>), and prostaglandin F<sub>2</sub>α (PGF<sub>2</sub>α) in arachidonic acid (AA) metabolism was inhibited, and the conversion of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) to anti-inflammatory mediators such as 8-hydroxyeicosapentaenoic acid (8-HEPE), 12-HEPE, and 15-HEPE was facilitated. Seven eicosanoic acid metabolites, 5-hydroxyeicosatetraenoic acid (5-HETE), 12-HETE, 20-HETE, PGD<sub>2</sub>, PGF<sub>2</sub>α, 5,6-epoxyeicosatrienoic acid (5,6-EET), and 12-HEPE, were considered to be physiologically important in attenuation of UC development in mice. These findings provided a metabolic-level theoretical basis for the preventive application of phosphorylated polysaccharides in inflammatory bowel disease.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"78 ","pages":"Article 108460"},"PeriodicalIF":5.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146192456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An infant-derived Bifidobacterium pseudocatenulatum DPU-MWF3-6 strain exhibits favorable in vitro functional properties and is well tolerated during early life in neonatal rats","authors":"Zhao Zhang, Yanchen Liu, Fanhua KONG, Yinglong Song, Yaowei Liu, Xiaomeng Wu, Guangqing Mu","doi":"10.1016/j.fbio.2026.108749","DOIUrl":"https://doi.org/10.1016/j.fbio.2026.108749","url":null,"abstract":"","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"79 1","pages":"108749-108749"},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food BiosciencePub Date : 2026-03-24DOI: 10.1016/j.fbio.2026.108727
Hailing Du, Zhen Du, Mengxin Ren, Yajing Wang, Zhexue Song, Zhili Xiong
{"title":"Rapid and comprehensive profiling of α-glucosidase and pancreatic lipase inhibitors in Eleutherococcus sessiliflorus fruit by AUF-UHPLC-Q-Orbitrap HRMS combined with molecular docking","authors":"Hailing Du, Zhen Du, Mengxin Ren, Yajing Wang, Zhexue Song, Zhili Xiong","doi":"10.1016/j.fbio.2026.108727","DOIUrl":"https://doi.org/10.1016/j.fbio.2026.108727","url":null,"abstract":"","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"79 1","pages":"108727-108727"},"PeriodicalIF":0.0,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}